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Greg G Goss - One of the best experts on this subject based on the ideXlab platform.
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microtubule dependent relocation of branchial v h atpase to the basolateral membrane in the pacific spiny dogfish squalus acanthias a role in base secretion
The Journal of Experimental Biology, 2006Co-Authors: Martin Tresguerres, Scott K Parks, Fumi Katoh, Greg G GossAbstract:We have previously shown that continuous intravenous infusion of NaHCO3 for 24 h ( approximately 1000 micromol kg(-1) h(-1)) results in the relocation of V-H+-ATPase from the cytoplasm to the basolateral membrane in the gills of the Pacific dogfish. To further investigate this putative base-secretive process we performed similar experiments with the addition of Colchicine, an inhibitor of cytoskeleton-dependent cellular trafficking processes. Blood pH and plasma total CO2 were significantly higher in the Colchicines-treated, HCO3- -infused fish compared with fish infused with HCO3- alone. The effect of Colchicine was highest after 24 h of infusion (8.33+/-0.06 vs 8.02+/-0.03 pH units, 15.72+/-3.29 vs 6.74+/-1.34 mmol CO2 l(-1), N=5). Immunohistochemistry and western blotting confirmed that Colchicine blocked the transit of V-H+-ATPase to the basolateral membrane. Furthermore, western blotting analyses from whole gill and cell membrane samples suggest that the short-term (6 h) response to alkaline stress consists of relocation of V-H+-ATPases already present in the cell to the basolateral membrane, while in the longer term (24 h) there is both relocation of preexistent enzyme and upregulation in the synthesis of new units. Our results strongly suggest that cellular relocation of V-H+-ATPase is necessary for enhanced HCO3- secretion across the gills of the Pacific dogfish.
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microtubule dependent relocation of branchial v h atpase to the basolateral membrane in the pacific spiny dogfish squalus acanthias a role in base secretion
The Journal of Experimental Biology, 2006Co-Authors: Martin Tresguerres, Scott K Parks, Fumi Katoh, Greg G GossAbstract:SUMMARY We have previously shown that continuous intravenous infusion of NaHCO 3 for 24 h (∼1000 μmol kg -1 h -1 ) results in the relocation of V-H + -ATPase from the cytoplasm to the basolateral membrane in the gills of the Pacific dogfish. To further investigate this putative base-secretive process we performed similar experiments with the addition of Colchicine, an inhibitor of cytoskeleton-dependent cellular trafficking processes. Blood pH and plasma total CO 2 were significantly higher in the Colchicines-treated, HCO 3 - -infused fish compared with fish infused with HCO 3 - alone. The effect of Colchicine was highest after 24 h of infusion (8.33±0.06 vs 8.02±0.03 pH units, 15.72±3.29 vs 6.74±1.34 mmol CO 2 l -1 , N =5). Immunohistochemistry and western blotting confirmed that Colchicine blocked the transit of V-H + -ATPase to the basolateral membrane. Furthermore, western blotting analyses from whole gill and cell membrane samples suggest that the short-term (6 h) response to alkaline stress consists of relocation of V-H + -ATPases already present in the cell to the basolateral membrane, while in the longer term (24 h) there is both relocation of preexistent enzyme and upregulation in the synthesis of new units. Our results strongly suggest that cellular relocation of V-H + -ATPase is necessary for enhanced HCO 3 - secretion across the gills of the Pacific dogfish.
Martin Tresguerres - One of the best experts on this subject based on the ideXlab platform.
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microtubule dependent relocation of branchial v h atpase to the basolateral membrane in the pacific spiny dogfish squalus acanthias a role in base secretion
The Journal of Experimental Biology, 2006Co-Authors: Martin Tresguerres, Scott K Parks, Fumi Katoh, Greg G GossAbstract:We have previously shown that continuous intravenous infusion of NaHCO3 for 24 h ( approximately 1000 micromol kg(-1) h(-1)) results in the relocation of V-H+-ATPase from the cytoplasm to the basolateral membrane in the gills of the Pacific dogfish. To further investigate this putative base-secretive process we performed similar experiments with the addition of Colchicine, an inhibitor of cytoskeleton-dependent cellular trafficking processes. Blood pH and plasma total CO2 were significantly higher in the Colchicines-treated, HCO3- -infused fish compared with fish infused with HCO3- alone. The effect of Colchicine was highest after 24 h of infusion (8.33+/-0.06 vs 8.02+/-0.03 pH units, 15.72+/-3.29 vs 6.74+/-1.34 mmol CO2 l(-1), N=5). Immunohistochemistry and western blotting confirmed that Colchicine blocked the transit of V-H+-ATPase to the basolateral membrane. Furthermore, western blotting analyses from whole gill and cell membrane samples suggest that the short-term (6 h) response to alkaline stress consists of relocation of V-H+-ATPases already present in the cell to the basolateral membrane, while in the longer term (24 h) there is both relocation of preexistent enzyme and upregulation in the synthesis of new units. Our results strongly suggest that cellular relocation of V-H+-ATPase is necessary for enhanced HCO3- secretion across the gills of the Pacific dogfish.
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microtubule dependent relocation of branchial v h atpase to the basolateral membrane in the pacific spiny dogfish squalus acanthias a role in base secretion
The Journal of Experimental Biology, 2006Co-Authors: Martin Tresguerres, Scott K Parks, Fumi Katoh, Greg G GossAbstract:SUMMARY We have previously shown that continuous intravenous infusion of NaHCO 3 for 24 h (∼1000 μmol kg -1 h -1 ) results in the relocation of V-H + -ATPase from the cytoplasm to the basolateral membrane in the gills of the Pacific dogfish. To further investigate this putative base-secretive process we performed similar experiments with the addition of Colchicine, an inhibitor of cytoskeleton-dependent cellular trafficking processes. Blood pH and plasma total CO 2 were significantly higher in the Colchicines-treated, HCO 3 - -infused fish compared with fish infused with HCO 3 - alone. The effect of Colchicine was highest after 24 h of infusion (8.33±0.06 vs 8.02±0.03 pH units, 15.72±3.29 vs 6.74±1.34 mmol CO 2 l -1 , N =5). Immunohistochemistry and western blotting confirmed that Colchicine blocked the transit of V-H + -ATPase to the basolateral membrane. Furthermore, western blotting analyses from whole gill and cell membrane samples suggest that the short-term (6 h) response to alkaline stress consists of relocation of V-H + -ATPases already present in the cell to the basolateral membrane, while in the longer term (24 h) there is both relocation of preexistent enzyme and upregulation in the synthesis of new units. Our results strongly suggest that cellular relocation of V-H + -ATPase is necessary for enhanced HCO 3 - secretion across the gills of the Pacific dogfish.
Jean Michel Scherrmann - One of the best experts on this subject based on the ideXlab platform.
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Colchicine nonresponsiveness in familial mediterranean fever: clinical, genetic, pharmacokinetic, and socioeconomic characterization
Seminars in Arthritis and Rheumatism, 2004Co-Authors: Merav Lidar, Elisabeth Niel, Ruth Gershoni-baruch, Yael Shinar, Jean Michel Scherrmann, Angela Chetrit, Pnina Langevitz, Avi LivnehAbstract:Abstract Objectives To identify the ethnic, clinical, genetic, and pharmacokinetic correlates of Colchicine treatment failure in patients with familial Mediterranean fever (FMF). Methods Fifty-nine FMF patients, unresponsive to a daily dose of ≥2 mg Colchicine, were compared with 51 Colchicine-responsive patients by clinical, demographic, and socioeconomic assessment, FMF gene (MEditerranean FeVer [ MEFV ]) mutation and serum amyloid A1 ( SAA1 ) gene polymorphism analysis, and plasma and white blood cell Colchicine level determination. Results Colchicine responders and nonresponders were comparable with respect to gender, age, duration and onset of the disease, and various demographic parameters. The 2 cohorts were found to carry mainly the M694V MEFV mutation and had a similar number of homozygotes or compound heterozygotes. Predominance of the α/β alleles of SAA1 and comparable plasma and polymorphonuclear Colchicine concentrations characterized both groups. Nonresponders were from lower socioeconomic backgrounds, had less education, and a more severe form of disease. A statistically significant 2-fold elevation of Colchicine concentration in the mononuclear cells (MNC) of responders was found. Conclusions Colchicine treatment failure in FMF is associated with inadequate Colchicine MNC concentration, probably resulting from a genetic defect unrelated to the underlying FMF.
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Brief report: treatment of severe Colchicine overdose with Colchicine-specific Fab fragments.
The New England journal of medicine, 1995Co-Authors: Jean Michel Scherrmann, Frédéric J. Baud, A. Sabouraud, Eric Vicaut, P. Taboulet, Jean Lang, Chantal Bismuth, Jean Marc RouziouxAbstract:The use of Colchicine is limited by its toxicity,1 and Colchicine overdose is associated with a high mortality rate. Patients with early hemodynamic collapse due to Colchicine overdose have particularly poor prognoses,2,3 and there has been no effective treatment for this complication of severe Colchicine intoxication.4 Colchicine binds reversibly to tubulin,5 and Colchicine-specific antibodies have been shown to restore the activity of tubulin in vitro.6,7 Colchicine-specific active immunization in rabbits and passive immunization in mice with substoichiometric doses of goat Colchicine-specific antibodies and their Fab fragments have been shown to prevent or reverse acute Colchicine poisoning.8–11 These . . .
Fumi Katoh - One of the best experts on this subject based on the ideXlab platform.
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microtubule dependent relocation of branchial v h atpase to the basolateral membrane in the pacific spiny dogfish squalus acanthias a role in base secretion
The Journal of Experimental Biology, 2006Co-Authors: Martin Tresguerres, Scott K Parks, Fumi Katoh, Greg G GossAbstract:We have previously shown that continuous intravenous infusion of NaHCO3 for 24 h ( approximately 1000 micromol kg(-1) h(-1)) results in the relocation of V-H+-ATPase from the cytoplasm to the basolateral membrane in the gills of the Pacific dogfish. To further investigate this putative base-secretive process we performed similar experiments with the addition of Colchicine, an inhibitor of cytoskeleton-dependent cellular trafficking processes. Blood pH and plasma total CO2 were significantly higher in the Colchicines-treated, HCO3- -infused fish compared with fish infused with HCO3- alone. The effect of Colchicine was highest after 24 h of infusion (8.33+/-0.06 vs 8.02+/-0.03 pH units, 15.72+/-3.29 vs 6.74+/-1.34 mmol CO2 l(-1), N=5). Immunohistochemistry and western blotting confirmed that Colchicine blocked the transit of V-H+-ATPase to the basolateral membrane. Furthermore, western blotting analyses from whole gill and cell membrane samples suggest that the short-term (6 h) response to alkaline stress consists of relocation of V-H+-ATPases already present in the cell to the basolateral membrane, while in the longer term (24 h) there is both relocation of preexistent enzyme and upregulation in the synthesis of new units. Our results strongly suggest that cellular relocation of V-H+-ATPase is necessary for enhanced HCO3- secretion across the gills of the Pacific dogfish.
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microtubule dependent relocation of branchial v h atpase to the basolateral membrane in the pacific spiny dogfish squalus acanthias a role in base secretion
The Journal of Experimental Biology, 2006Co-Authors: Martin Tresguerres, Scott K Parks, Fumi Katoh, Greg G GossAbstract:SUMMARY We have previously shown that continuous intravenous infusion of NaHCO 3 for 24 h (∼1000 μmol kg -1 h -1 ) results in the relocation of V-H + -ATPase from the cytoplasm to the basolateral membrane in the gills of the Pacific dogfish. To further investigate this putative base-secretive process we performed similar experiments with the addition of Colchicine, an inhibitor of cytoskeleton-dependent cellular trafficking processes. Blood pH and plasma total CO 2 were significantly higher in the Colchicines-treated, HCO 3 - -infused fish compared with fish infused with HCO 3 - alone. The effect of Colchicine was highest after 24 h of infusion (8.33±0.06 vs 8.02±0.03 pH units, 15.72±3.29 vs 6.74±1.34 mmol CO 2 l -1 , N =5). Immunohistochemistry and western blotting confirmed that Colchicine blocked the transit of V-H + -ATPase to the basolateral membrane. Furthermore, western blotting analyses from whole gill and cell membrane samples suggest that the short-term (6 h) response to alkaline stress consists of relocation of V-H + -ATPases already present in the cell to the basolateral membrane, while in the longer term (24 h) there is both relocation of preexistent enzyme and upregulation in the synthesis of new units. Our results strongly suggest that cellular relocation of V-H + -ATPase is necessary for enhanced HCO 3 - secretion across the gills of the Pacific dogfish.
Scott K Parks - One of the best experts on this subject based on the ideXlab platform.
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microtubule dependent relocation of branchial v h atpase to the basolateral membrane in the pacific spiny dogfish squalus acanthias a role in base secretion
The Journal of Experimental Biology, 2006Co-Authors: Martin Tresguerres, Scott K Parks, Fumi Katoh, Greg G GossAbstract:We have previously shown that continuous intravenous infusion of NaHCO3 for 24 h ( approximately 1000 micromol kg(-1) h(-1)) results in the relocation of V-H+-ATPase from the cytoplasm to the basolateral membrane in the gills of the Pacific dogfish. To further investigate this putative base-secretive process we performed similar experiments with the addition of Colchicine, an inhibitor of cytoskeleton-dependent cellular trafficking processes. Blood pH and plasma total CO2 were significantly higher in the Colchicines-treated, HCO3- -infused fish compared with fish infused with HCO3- alone. The effect of Colchicine was highest after 24 h of infusion (8.33+/-0.06 vs 8.02+/-0.03 pH units, 15.72+/-3.29 vs 6.74+/-1.34 mmol CO2 l(-1), N=5). Immunohistochemistry and western blotting confirmed that Colchicine blocked the transit of V-H+-ATPase to the basolateral membrane. Furthermore, western blotting analyses from whole gill and cell membrane samples suggest that the short-term (6 h) response to alkaline stress consists of relocation of V-H+-ATPases already present in the cell to the basolateral membrane, while in the longer term (24 h) there is both relocation of preexistent enzyme and upregulation in the synthesis of new units. Our results strongly suggest that cellular relocation of V-H+-ATPase is necessary for enhanced HCO3- secretion across the gills of the Pacific dogfish.
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microtubule dependent relocation of branchial v h atpase to the basolateral membrane in the pacific spiny dogfish squalus acanthias a role in base secretion
The Journal of Experimental Biology, 2006Co-Authors: Martin Tresguerres, Scott K Parks, Fumi Katoh, Greg G GossAbstract:SUMMARY We have previously shown that continuous intravenous infusion of NaHCO 3 for 24 h (∼1000 μmol kg -1 h -1 ) results in the relocation of V-H + -ATPase from the cytoplasm to the basolateral membrane in the gills of the Pacific dogfish. To further investigate this putative base-secretive process we performed similar experiments with the addition of Colchicine, an inhibitor of cytoskeleton-dependent cellular trafficking processes. Blood pH and plasma total CO 2 were significantly higher in the Colchicines-treated, HCO 3 - -infused fish compared with fish infused with HCO 3 - alone. The effect of Colchicine was highest after 24 h of infusion (8.33±0.06 vs 8.02±0.03 pH units, 15.72±3.29 vs 6.74±1.34 mmol CO 2 l -1 , N =5). Immunohistochemistry and western blotting confirmed that Colchicine blocked the transit of V-H + -ATPase to the basolateral membrane. Furthermore, western blotting analyses from whole gill and cell membrane samples suggest that the short-term (6 h) response to alkaline stress consists of relocation of V-H + -ATPases already present in the cell to the basolateral membrane, while in the longer term (24 h) there is both relocation of preexistent enzyme and upregulation in the synthesis of new units. Our results strongly suggest that cellular relocation of V-H + -ATPase is necessary for enhanced HCO 3 - secretion across the gills of the Pacific dogfish.
