The Experts below are selected from a list of 61263 Experts worldwide ranked by ideXlab platform
Jianfa Wang - One of the best experts on this subject based on the ideXlab platform.
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Characterization of Gut Microbiota in Prenatal Cold Stress Offspring Rats by 16S rRNA Sequencing
Animals : an open access journal from MDPI, 2020Co-Authors: Jiasan Zheng, Jianfa Wang, Tingting Zhu, Lipeng Wang, Lian ShuaiAbstract:Our previous study showed a reduction of anxiety-like behavior in offspring rats suffered from prenatal Cold Stress; whether this was related to changes in the offspring gut microbiota is unclear. To obtain the evidence for the role of the gut microbiota in prenatal Cold Stress offspring, 16S rRNA sequencing technology was used. Male and female offspring rat feces were collected from a room temperature group and a prenatal Cold Stress group (n ≥ 8) for microbial DNA extraction, followed by 16S rRNA sequencing. The results indicated that prenatal Cold Stress could change the offspring's gut microbiota composition. Prenatal Cold Stress significantly upregulates Lactobacillus, Lactobacillus_gasseri, Bacteroides, and Bacteroides-acidifaciens in female offspring, whereas prenatal Cold Stress significantly reduced Lachnospiraceae and Prevotellaceae in male offspring. These data showed the characterization of gut microbiota in prenatal Cold Stress offspring rats, and these data suggest that microbiological intervention in the future can potentially prevent the negative effects caused by Cold Stress to animals.
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Effects of prenatal Cold Stress on maternal serum metabolomics in rats
Life sciences, 2020Co-Authors: Shuai Lian, Di Wang, Xueheng Guo, Huanmin Yang, Jianfa WangAbstract:Our previous studies have shown that prenatal Cold Stress leads to placental inflammatory response and induces anxiety-like behavior reduced in offspring rats. However, the role and mechanisms by which prenatal Cold Stress affects offspring remain unclear. The aim of this study was to determine the metabolic profiles from the maternal serum and helpful in understanding the role and mechanisms by which prenatal Cold Stress affects the offspring. In this study, liquid chromatography-mass spectrometry (LC/MS) was used to analyze serum metabolites, and PCA, PLS-DA, and OPLS-DA were performed to analyze changes in metabolites in the maternal serum after Cold Stress of 3 or 7 days. The results showed that 19 metabolites in the CS (Cold Stress 7 days)-NS (control) group and 23 metabolites in the CT (Cold Stress 3 days)-NT (control) group were significantly altered. These metabolites were mainly associated with unsaturated fatty acid synthesis, and arachidonic acid, linoleic acid, and glutamine and glutamate metabolism. The data indicated that prenatal Cold Stress not only affected the maternal neuroendocrine system, but also affected the immune system, and lipid and amino acid metabolism. These results further supported the findings of our previous studies on the effects of prenatal Cold Stress on the mother and offspring. A more comprehensive understanding of these data may lead to maternal intervention that can reverse the damage of prenatal Stressors.
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Response of the maternal hypothalamus to Cold Stress during late pregnancy in rats
Brain research, 2019Co-Authors: Di Wang, Huanmin Yang, Jianfa Wang, Hai Wang, Jingru Guo, Shuai LianAbstract:Maternal Stress is a key risk factor in the development of offspring. We previously identified prenatal Cold Stress-induced anxiety-like behavior reduced in the offspring of rats along with negative feedback regulation from the maternal hippocampus on the hypothalamic-pituitary-adrenal (HPA) axis during prenatal Cold Stress. However, the precise function of the maternal hypothalamus response to Cold Stress during late pregnancy in rats has not yet been determined. Therefore, we examined proteins in the hypothalamus that respond to aldosterone, neurodevelopment, inflammation and apoptosis. Our results show that prenatal Cold Stress induced the expression of mineralocorticoid receptors (MR) and 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2), suggesting prenatal Cold Stress may promote the elevation of aldosterone levels in the hypothalamus. Remarkably, increased expression of brain derived neurotrophic factor (BDNF) helped to replenish intracellular peptidergic stores and ensure homeostatic balance during prenatal Cold Stress. Furthermore, prenatal Cold Stress reduced the expression of c-Fos via STAT3 and ERK1/2 pathways in the hypothalamus. Moreover, prenatal Cold Stress induced NF-κB phosphorylation at Ser536, then promoted the expression of inducible nitric oxide synthase (iNOS) and induced an apoptosis-related protein response. Together, this study confirms that changes in the maternal hypothalamus during Cold Stress in late pregnancy are directly reflective of the response of the HPA to Cold Stress and demonstrates how the hypothalamus coordinates Cold Stress. We suggest mechanisms which might explain how these states might be linked with an abnormal Stress response.
Shuai Lian - One of the best experts on this subject based on the ideXlab platform.
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Effects of prenatal Cold Stress on maternal serum metabolomics in rats
Life sciences, 2020Co-Authors: Shuai Lian, Di Wang, Xueheng Guo, Huanmin Yang, Jianfa WangAbstract:Our previous studies have shown that prenatal Cold Stress leads to placental inflammatory response and induces anxiety-like behavior reduced in offspring rats. However, the role and mechanisms by which prenatal Cold Stress affects offspring remain unclear. The aim of this study was to determine the metabolic profiles from the maternal serum and helpful in understanding the role and mechanisms by which prenatal Cold Stress affects the offspring. In this study, liquid chromatography-mass spectrometry (LC/MS) was used to analyze serum metabolites, and PCA, PLS-DA, and OPLS-DA were performed to analyze changes in metabolites in the maternal serum after Cold Stress of 3 or 7 days. The results showed that 19 metabolites in the CS (Cold Stress 7 days)-NS (control) group and 23 metabolites in the CT (Cold Stress 3 days)-NT (control) group were significantly altered. These metabolites were mainly associated with unsaturated fatty acid synthesis, and arachidonic acid, linoleic acid, and glutamine and glutamate metabolism. The data indicated that prenatal Cold Stress not only affected the maternal neuroendocrine system, but also affected the immune system, and lipid and amino acid metabolism. These results further supported the findings of our previous studies on the effects of prenatal Cold Stress on the mother and offspring. A more comprehensive understanding of these data may lead to maternal intervention that can reverse the damage of prenatal Stressors.
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Response of the maternal hypothalamus to Cold Stress during late pregnancy in rats
Brain research, 2019Co-Authors: Di Wang, Huanmin Yang, Jianfa Wang, Hai Wang, Jingru Guo, Shuai LianAbstract:Maternal Stress is a key risk factor in the development of offspring. We previously identified prenatal Cold Stress-induced anxiety-like behavior reduced in the offspring of rats along with negative feedback regulation from the maternal hippocampus on the hypothalamic-pituitary-adrenal (HPA) axis during prenatal Cold Stress. However, the precise function of the maternal hypothalamus response to Cold Stress during late pregnancy in rats has not yet been determined. Therefore, we examined proteins in the hypothalamus that respond to aldosterone, neurodevelopment, inflammation and apoptosis. Our results show that prenatal Cold Stress induced the expression of mineralocorticoid receptors (MR) and 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2), suggesting prenatal Cold Stress may promote the elevation of aldosterone levels in the hypothalamus. Remarkably, increased expression of brain derived neurotrophic factor (BDNF) helped to replenish intracellular peptidergic stores and ensure homeostatic balance during prenatal Cold Stress. Furthermore, prenatal Cold Stress reduced the expression of c-Fos via STAT3 and ERK1/2 pathways in the hypothalamus. Moreover, prenatal Cold Stress induced NF-κB phosphorylation at Ser536, then promoted the expression of inducible nitric oxide synthase (iNOS) and induced an apoptosis-related protein response. Together, this study confirms that changes in the maternal hypothalamus during Cold Stress in late pregnancy are directly reflective of the response of the HPA to Cold Stress and demonstrates how the hypothalamus coordinates Cold Stress. We suggest mechanisms which might explain how these states might be linked with an abnormal Stress response.
Jinyuan Liu - One of the best experts on this subject based on the ideXlab platform.
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a proteomic analysis of Cold Stress responses in rice seedlings
Proteomics, 2005Co-Authors: Suxia Cui, Fang Huang, Jie Wang, Yongsheng Cheng, Jinyuan LiuAbstract:Using proteomic analysis, an investigation aimed at a better understanding of the molecular adaptation mechanisms of Cold Stress was carried out in rice (Oryza sativa). The seedlings were exposed to a progressively low temperature Stress treatment from normal temperature to 15, 10, and 5°C. Proteins were extracted from the leaves collected from both control and Stressed seedlings. By fractionation, approximately 1700 protein spots were separated and visualized on CBB-stained 2-D gels. Sixty protein spots were found to be up-regulated in responding to the progressively low temperature Stress and displayed different dynamic patterns. As an initial work, 41 of these proteins were identified using MALDI-TOF MS or ESI/MS/MS. These Cold responsive proteins, besides two proteins of unknown function, include four factors of protein biosynthesis, four molecular chaperones, two proteases, and eight enzymes involved in biosynthesis of cell wall components, seven antioxidative/detoxifying enzymes, and proteins linked to energy pathway, as well as a protein involved in signal transduction. The functional proteomes illuminate the facts, at least in plant cell, that protein quality control mediated by chaperones and proteases and enhancement of cell wall components play important roles in tolerance to Cold Stress. Using TargetP program, the subcellular localization of the identified proteins was analyzed. Proteins (43.9%) were predicted to be located in the chloroplasts, implying that chloroplast proteome is virtually subjective to Cold Stress. The physiological implications, revealed from the experimental data, are discussed in context of a complex metabolic network in plant cells responsive to Cold Stress.
Setsuko Komatsu - One of the best experts on this subject based on the ideXlab platform.
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Proteomic analysis of rice seedlings during Cold Stress
Proteomics, 2007Co-Authors: Makoto Hashimoto, Setsuko KomatsuAbstract:Low temperature is one of the important environmental changes that affect plant growth and agricultural production. To investigate the responses of rice to Cold Stress, changes in protein expression were analyzed using a proteomic approach. Two-week-old rice seedlings were exposed to 5 degrees C for 48 h, then total crude proteins were extracted from leaf blades, leaf sheaths and roots, separated by 2-DE and stained with CBB. Of the 250-400 protein spots from each organ, 39 proteins changed in abundance after Cold Stress, with 19 proteins increasing, and 20 proteins decreasing. In leaf blades, it was difficult to detect the changes in Stress-responsive proteins due to the presence of an abundant protein, ribulose bisphosphate carboxylase/oxygenase large subunit (RuBisCO LSU), which accounted for about 50% of the total proteins. To overcome this problem, an antibody-affinity column was prepared to trap RuBisCO LSU, and the remaining proteins in the flow through from the column were subsequently separated using 2-DE. As a result, slight changes in Stress responsive proteins were clearly displayed, and four proteins were newly detected after Cold Stress. From identified proteins, it was concluded that proteins related to energy metabolism were up-regulated, and defense-related proteins were down-regulated in leaf blades, by Cold Stress. These results suggest that energy production is activated in the chilling environment; furthermore, Stress-related proteins are rapidly up-regulated, while defense-related proteins disappear, under long-term Cold Stress.
Di Wang - One of the best experts on this subject based on the ideXlab platform.
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Effects of prenatal Cold Stress on maternal serum metabolomics in rats
Life sciences, 2020Co-Authors: Shuai Lian, Di Wang, Xueheng Guo, Huanmin Yang, Jianfa WangAbstract:Our previous studies have shown that prenatal Cold Stress leads to placental inflammatory response and induces anxiety-like behavior reduced in offspring rats. However, the role and mechanisms by which prenatal Cold Stress affects offspring remain unclear. The aim of this study was to determine the metabolic profiles from the maternal serum and helpful in understanding the role and mechanisms by which prenatal Cold Stress affects the offspring. In this study, liquid chromatography-mass spectrometry (LC/MS) was used to analyze serum metabolites, and PCA, PLS-DA, and OPLS-DA were performed to analyze changes in metabolites in the maternal serum after Cold Stress of 3 or 7 days. The results showed that 19 metabolites in the CS (Cold Stress 7 days)-NS (control) group and 23 metabolites in the CT (Cold Stress 3 days)-NT (control) group were significantly altered. These metabolites were mainly associated with unsaturated fatty acid synthesis, and arachidonic acid, linoleic acid, and glutamine and glutamate metabolism. The data indicated that prenatal Cold Stress not only affected the maternal neuroendocrine system, but also affected the immune system, and lipid and amino acid metabolism. These results further supported the findings of our previous studies on the effects of prenatal Cold Stress on the mother and offspring. A more comprehensive understanding of these data may lead to maternal intervention that can reverse the damage of prenatal Stressors.
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Response of the maternal hypothalamus to Cold Stress during late pregnancy in rats
Brain research, 2019Co-Authors: Di Wang, Huanmin Yang, Jianfa Wang, Hai Wang, Jingru Guo, Shuai LianAbstract:Maternal Stress is a key risk factor in the development of offspring. We previously identified prenatal Cold Stress-induced anxiety-like behavior reduced in the offspring of rats along with negative feedback regulation from the maternal hippocampus on the hypothalamic-pituitary-adrenal (HPA) axis during prenatal Cold Stress. However, the precise function of the maternal hypothalamus response to Cold Stress during late pregnancy in rats has not yet been determined. Therefore, we examined proteins in the hypothalamus that respond to aldosterone, neurodevelopment, inflammation and apoptosis. Our results show that prenatal Cold Stress induced the expression of mineralocorticoid receptors (MR) and 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2), suggesting prenatal Cold Stress may promote the elevation of aldosterone levels in the hypothalamus. Remarkably, increased expression of brain derived neurotrophic factor (BDNF) helped to replenish intracellular peptidergic stores and ensure homeostatic balance during prenatal Cold Stress. Furthermore, prenatal Cold Stress reduced the expression of c-Fos via STAT3 and ERK1/2 pathways in the hypothalamus. Moreover, prenatal Cold Stress induced NF-κB phosphorylation at Ser536, then promoted the expression of inducible nitric oxide synthase (iNOS) and induced an apoptosis-related protein response. Together, this study confirms that changes in the maternal hypothalamus during Cold Stress in late pregnancy are directly reflective of the response of the HPA to Cold Stress and demonstrates how the hypothalamus coordinates Cold Stress. We suggest mechanisms which might explain how these states might be linked with an abnormal Stress response.