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Katsuyoshi Matsunami - One of the best experts on this subject based on the ideXlab platform.
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Anti-Aging Properties of Curcuma Heynena Valeton & Zipj: A Scientific Approach to its use in Javanese tradition
Elsevier B.V., 2018Co-Authors: Idha Kusumawati, Kresma Oky Kurniawan, Subhan Rullyansyah, Eka Pramyrtha Hestianah, Tri Anggono Prijo, Retno Widyowati, Juni Ekowati, Suprapto Maat, Katsuyoshi MatsunamiAbstract:Ethnopharmacological Relevance: Temu Giring, The Rhizome Of Curcuma Heyneana, is native to tropical regions, especially in Indonesia. It is traditionally used as a skin care, for cosmetic and body freshness, in Javanese and Balinese women, and has many other bioactivities such as antioxidant, anticancer and antiinflammatory. Aim of the study: The purpose of this study was to determine the antiaging activity of C. heyneana to prove its traditional use. Materials and methods: The antioxidant activity was determined using the DPPH free radical method, and anti- aging activity was examined using in vitro assays such as tyrosinase Inhibitor and Collagenase Inhibitor. In vivo tests were performed by observing histomorphologic changes in rat skin exposed to Ultraviolet (UV) rays. The total curcuminoid contents and chromatographic profiles were determined by Thin Layer Chromatography (TLC) – densitometry. Results: In all in vitro assays, all of the extracts showed a dose-dependent manner in the final concentration range from 62.5 to 500 µg/mL for DPPH assay and 31.25 to 250 µg/mL for tyrosinase inhibition and Collagenase inhibition assay. Curcuminoid (CUR), the active principle of Curcuma genus shows antioxidant, tyrosinase in- hibitor and Collagenase Inhibitor activity greater than all C. Heyneana extracts. The in vivo assay results showed that the topical application of the crude extract of C. Heyneana produced significant improvement effects on the UV-induced skin structure damage. The total CUR content was correlated with the anti-aging activity of Curcuma Heyneana. Conclusions: The results show that C. Heyneana contains antioxidant compounds and has potent anti-aging ac- tivity, indicating that it can be used as an anti-aging drug candidate or as a phyto-cosmeceutical. Keywords: Curcuma Anti aging Antioxidants, Collagenase Inhibitor Tyrosinase Inhibitor Rat skin histomorphologic change
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Anti-aging properties of Curcuma heyneana Valeton & Zipj: A scientific approach to its use in Javanese tradition.
Journal of ethnopharmacology, 2018Co-Authors: Idha Kusumawati, Kresma Oky Kurniawan, Subhan Rullyansyah, Eka Pramyrtha Hestianah, Tri Anggono Prijo, Retno Widyowati, Juni Ekowati, Suprapto Maat, Katsuyoshi MatsunamiAbstract:Abstract Ethnopharmacological relevance Temu giring, the rhizome of Curcuma heyneana Valeton & Zipj (C. heyneana), is native to tropical regions, especially in Indonesia. It is traditionally used as a skin care, for cosmetic and body freshness, in Javanese and Balinese women, and has many other bioactivities such as antioxidant, anticancer and antiinflammatory. Aim of the study The purpose of this study was to determine the antiaging activity of C. heyneana to prove its traditional use. Materials and methods The antioxidant activity was determined using the DPPH free radical method, and anti-aging activity was examined using in vitro assays such as tyrosinase Inhibitor and Collagenase Inhibitor. In vivo tests were performed by observing histomorphologic changes in rat skin exposed to Ultraviolet (UV) rays. The total curcuminoid contents and chromatographic profiles were determined by Thin Layer Chromatography (TLC) – densitometry. Results In all in vitro assays, all of the extracts showed a dose-dependent manner in the final concentration range from 62.5 to 500 µg/mL for DPPH assay and 31.25 to 250 µg/mL for tyrosinase inhibition and Collagenase inhibition assay. Curcuminoid (CUR), the active principle of Curcuma genus shows antioxidant, tyrosinase Inhibitor and Collagenase Inhibitor activity greater than all C. heyneana extracts. The in vivo assay results showed that the topical application of the crude extract of C. heyneana produced significant improvement effects on the UV-induced skin structure damage. The total CUR content was correlated with the anti-aging activity of Curcuma heyneana. Conclusions The results show that C. heyneana contains antioxidant compounds and has potent anti-aging activity, indicating that it can be used as an anti-aging drug candidate or as a phyto-cosmeceutical.
Idha Kusumawati - One of the best experts on this subject based on the ideXlab platform.
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Anti-Aging Properties of Curcuma Heynena Valeton & Zipj: A Scientific Approach to its use in Javanese tradition
Elsevier B.V., 2018Co-Authors: Idha Kusumawati, Kresma Oky Kurniawan, Subhan Rullyansyah, Eka Pramyrtha Hestianah, Tri Anggono Prijo, Retno Widyowati, Juni Ekowati, Suprapto Maat, Katsuyoshi MatsunamiAbstract:Ethnopharmacological Relevance: Temu Giring, The Rhizome Of Curcuma Heyneana, is native to tropical regions, especially in Indonesia. It is traditionally used as a skin care, for cosmetic and body freshness, in Javanese and Balinese women, and has many other bioactivities such as antioxidant, anticancer and antiinflammatory. Aim of the study: The purpose of this study was to determine the antiaging activity of C. heyneana to prove its traditional use. Materials and methods: The antioxidant activity was determined using the DPPH free radical method, and anti- aging activity was examined using in vitro assays such as tyrosinase Inhibitor and Collagenase Inhibitor. In vivo tests were performed by observing histomorphologic changes in rat skin exposed to Ultraviolet (UV) rays. The total curcuminoid contents and chromatographic profiles were determined by Thin Layer Chromatography (TLC) – densitometry. Results: In all in vitro assays, all of the extracts showed a dose-dependent manner in the final concentration range from 62.5 to 500 µg/mL for DPPH assay and 31.25 to 250 µg/mL for tyrosinase inhibition and Collagenase inhibition assay. Curcuminoid (CUR), the active principle of Curcuma genus shows antioxidant, tyrosinase in- hibitor and Collagenase Inhibitor activity greater than all C. Heyneana extracts. The in vivo assay results showed that the topical application of the crude extract of C. Heyneana produced significant improvement effects on the UV-induced skin structure damage. The total CUR content was correlated with the anti-aging activity of Curcuma Heyneana. Conclusions: The results show that C. Heyneana contains antioxidant compounds and has potent anti-aging ac- tivity, indicating that it can be used as an anti-aging drug candidate or as a phyto-cosmeceutical. Keywords: Curcuma Anti aging Antioxidants, Collagenase Inhibitor Tyrosinase Inhibitor Rat skin histomorphologic change
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Anti-aging properties of Curcuma heyneana Valeton & Zipj: A scientific approach to its use in Javanese tradition.
Journal of ethnopharmacology, 2018Co-Authors: Idha Kusumawati, Kresma Oky Kurniawan, Subhan Rullyansyah, Eka Pramyrtha Hestianah, Tri Anggono Prijo, Retno Widyowati, Juni Ekowati, Suprapto Maat, Katsuyoshi MatsunamiAbstract:Abstract Ethnopharmacological relevance Temu giring, the rhizome of Curcuma heyneana Valeton & Zipj (C. heyneana), is native to tropical regions, especially in Indonesia. It is traditionally used as a skin care, for cosmetic and body freshness, in Javanese and Balinese women, and has many other bioactivities such as antioxidant, anticancer and antiinflammatory. Aim of the study The purpose of this study was to determine the antiaging activity of C. heyneana to prove its traditional use. Materials and methods The antioxidant activity was determined using the DPPH free radical method, and anti-aging activity was examined using in vitro assays such as tyrosinase Inhibitor and Collagenase Inhibitor. In vivo tests were performed by observing histomorphologic changes in rat skin exposed to Ultraviolet (UV) rays. The total curcuminoid contents and chromatographic profiles were determined by Thin Layer Chromatography (TLC) – densitometry. Results In all in vitro assays, all of the extracts showed a dose-dependent manner in the final concentration range from 62.5 to 500 µg/mL for DPPH assay and 31.25 to 250 µg/mL for tyrosinase inhibition and Collagenase inhibition assay. Curcuminoid (CUR), the active principle of Curcuma genus shows antioxidant, tyrosinase Inhibitor and Collagenase Inhibitor activity greater than all C. heyneana extracts. The in vivo assay results showed that the topical application of the crude extract of C. heyneana produced significant improvement effects on the UV-induced skin structure damage. The total CUR content was correlated with the anti-aging activity of Curcuma heyneana. Conclusions The results show that C. heyneana contains antioxidant compounds and has potent anti-aging activity, indicating that it can be used as an anti-aging drug candidate or as a phyto-cosmeceutical.
G. Murphy - One of the best experts on this subject based on the ideXlab platform.
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tissue Inhibitor of metalloproteinase 1 messenger rna expression is enhanced relative to interstitial Collagenase messenger rna in experimental liver injury and fibrosis
Hepatology, 1996Co-Authors: John P. Iredale, Rc Benyon, M J P Arthur, W F Ferris, R Alcolado, Paul J Winwood, N Clark, G. MurphyAbstract:Liver fibrosis results from a relative imbalance between synthesis and degradation of matrix proteins. We have previously described release of the protein Collagenase Inhibitor, tissue Inhibitor of metalloproteinase-1 (TIMP-1), by culture-activated human hepatic stellate cells (HSCs). In this study, we have investigated the relative expression of TIMP-1 and interstitial Collagenase in culture-activated rat HSCs and rat models of liver injury and fibrosis. The complementary DNA (cDNA) for rat TIMP-1 was obtained by homology polymerase chain reaction (PCR) and sequenced. By Northern analysis using this probe, TIMP-1 messenger RNA (mRNA) expression was up-regulated with HSC activation by culture on plastic as defined by cellular expression of procollagen-1. Interstitial Collagenase mRNA was expressed in early 1. Interstitial Collagenase mRNA was expressed in early culture (<4 days) but became undetectable in more activated cells (7-21 days). By activity assay of serum-free cell-conditioned media, TIMP-1 was found to be released in increasingly concentrations with duration of culture on plastic. Expression of TIMP-1 interstitial Collagenase, and procollagen-1 mRNAs were studied in rat models of biliary and parenchymal injury (bile duct ligation and CC14 administration) by ribonuclease protein assay. TIMP-1 mRNA expression was increased at 6, 24 hours, and 3 days after bile duct ligation and was also shown to rise in acute CC14 liver injury and remain elevated as the liver became fibrotic. TIMP-1 expression preceded procollagen-1 expression in both models. In contrasts, interstitial Collagenase mRNA levels remained similar to control values throughout both models of liver injury. Total cellular RNA from hepatocytes, HSCs, and kupffer cells freshly isolated from livers after acute CC14 injury was subjected to Northern analysis. TIMP-1 transcripts were observed in nonparenchymal cells only. We suggest that increased expression of TIMP-1 relative to interstitial Collagenase by HSCs may promote progression of liver fibrosis in these rat models by preventing degradation of secreted collagens.
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Tissue Inhibitor of Metalloproteinase-1 and Interstitial Collagenase Expression in Autoimmune Chronic Active Hepatitis and Activated Human Hepatic Lipocytes
Clinical science (London England : 1979), 1995Co-Authors: John P. Iredale, S. Goddard, G. Murphy, Rc Benyon, Michael J. P. ArthurAbstract:1. Activated hepatic lipocytes are central to the pathogenesis of liver fibrosis as the principal source of both interstitial collagens and matrix-degrading metalloproteinases. In progressive fibrosis there is a failure to degrade interstitial collagens with a reported decrease in Collagenase activity. In these studies we investigate expression of the potent Collagenase Inhibitor, tissue Inhibitor of metalloproteinase-1, and interstitial Collagenase in end-stage autoimmune chronic active hepatitis and activated human hepatic lipocytes in culture. 2. Messenger RNA transcripts for interstitial Collagenase and tissue Inhibitor of metalloproteinase-1 in explanted human liver were quantified by ribonuclease protection assay and densitometric analysis. This indicated that tissue Inhibitor of metalloproteinase-1 and interstitial Collagenase expression in autoimmune chronic active hepatitis were also co-ordinately up-regulated. 3. Using Northern analysis of RNA from human lipocytes in primary culture on plastic, mRNA for interstitial Collagenase could not be detected in unstimulated cells but was present after stimulation with tumour necrosis factor alpha. Tissue Inhibitor of metalloproteinase-1 mRNA was present in unstimulated lipocytes and up-regulated fivefold in response to tumour necrosis factor alpha. Using activity assay of serum-free conditioned media, interstitial Collagenase could not be detected in unstimulated primary cultures, primary cultures stimulated with tumour necrosis factor alpha or transforming growth factor beta-1 ( n = 3 and n = 4 respectively) or in passaged lipocytes ( n = 6). In contrast, free tissue Inhibitor of metalloproteinase-1 activity was present in unstimulated and passaged cultures and this was increased in response to tumour necrosis factor alpha and transforming growth factor beta-1. 4. These data indicate that for culture-activated lipocytes there is a net inhibition of matrix degradation mediated by excess tissue Inhibitor of metalloproteinase-1 expression relative to interstitial Collagenase. In autoimmune chronic active hepatitis, our messenger RNA studies demonstrate a similar up-regulation of tissue Inhibitor of metalloproteinase-1 in addition to interstitial Collagenase. This may contribute to the accumulation of interstitial collagens and progression of liver fibrosis in this disease.
Subhan Rullyansyah - One of the best experts on this subject based on the ideXlab platform.
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Anti-Aging Properties of Curcuma Heynena Valeton & Zipj: A Scientific Approach to its use in Javanese tradition
Elsevier B.V., 2018Co-Authors: Idha Kusumawati, Kresma Oky Kurniawan, Subhan Rullyansyah, Eka Pramyrtha Hestianah, Tri Anggono Prijo, Retno Widyowati, Juni Ekowati, Suprapto Maat, Katsuyoshi MatsunamiAbstract:Ethnopharmacological Relevance: Temu Giring, The Rhizome Of Curcuma Heyneana, is native to tropical regions, especially in Indonesia. It is traditionally used as a skin care, for cosmetic and body freshness, in Javanese and Balinese women, and has many other bioactivities such as antioxidant, anticancer and antiinflammatory. Aim of the study: The purpose of this study was to determine the antiaging activity of C. heyneana to prove its traditional use. Materials and methods: The antioxidant activity was determined using the DPPH free radical method, and anti- aging activity was examined using in vitro assays such as tyrosinase Inhibitor and Collagenase Inhibitor. In vivo tests were performed by observing histomorphologic changes in rat skin exposed to Ultraviolet (UV) rays. The total curcuminoid contents and chromatographic profiles were determined by Thin Layer Chromatography (TLC) – densitometry. Results: In all in vitro assays, all of the extracts showed a dose-dependent manner in the final concentration range from 62.5 to 500 µg/mL for DPPH assay and 31.25 to 250 µg/mL for tyrosinase inhibition and Collagenase inhibition assay. Curcuminoid (CUR), the active principle of Curcuma genus shows antioxidant, tyrosinase in- hibitor and Collagenase Inhibitor activity greater than all C. Heyneana extracts. The in vivo assay results showed that the topical application of the crude extract of C. Heyneana produced significant improvement effects on the UV-induced skin structure damage. The total CUR content was correlated with the anti-aging activity of Curcuma Heyneana. Conclusions: The results show that C. Heyneana contains antioxidant compounds and has potent anti-aging ac- tivity, indicating that it can be used as an anti-aging drug candidate or as a phyto-cosmeceutical. Keywords: Curcuma Anti aging Antioxidants, Collagenase Inhibitor Tyrosinase Inhibitor Rat skin histomorphologic change
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Anti-aging properties of Curcuma heyneana Valeton & Zipj: A scientific approach to its use in Javanese tradition.
Journal of ethnopharmacology, 2018Co-Authors: Idha Kusumawati, Kresma Oky Kurniawan, Subhan Rullyansyah, Eka Pramyrtha Hestianah, Tri Anggono Prijo, Retno Widyowati, Juni Ekowati, Suprapto Maat, Katsuyoshi MatsunamiAbstract:Abstract Ethnopharmacological relevance Temu giring, the rhizome of Curcuma heyneana Valeton & Zipj (C. heyneana), is native to tropical regions, especially in Indonesia. It is traditionally used as a skin care, for cosmetic and body freshness, in Javanese and Balinese women, and has many other bioactivities such as antioxidant, anticancer and antiinflammatory. Aim of the study The purpose of this study was to determine the antiaging activity of C. heyneana to prove its traditional use. Materials and methods The antioxidant activity was determined using the DPPH free radical method, and anti-aging activity was examined using in vitro assays such as tyrosinase Inhibitor and Collagenase Inhibitor. In vivo tests were performed by observing histomorphologic changes in rat skin exposed to Ultraviolet (UV) rays. The total curcuminoid contents and chromatographic profiles were determined by Thin Layer Chromatography (TLC) – densitometry. Results In all in vitro assays, all of the extracts showed a dose-dependent manner in the final concentration range from 62.5 to 500 µg/mL for DPPH assay and 31.25 to 250 µg/mL for tyrosinase inhibition and Collagenase inhibition assay. Curcuminoid (CUR), the active principle of Curcuma genus shows antioxidant, tyrosinase Inhibitor and Collagenase Inhibitor activity greater than all C. heyneana extracts. The in vivo assay results showed that the topical application of the crude extract of C. heyneana produced significant improvement effects on the UV-induced skin structure damage. The total CUR content was correlated with the anti-aging activity of Curcuma heyneana. Conclusions The results show that C. heyneana contains antioxidant compounds and has potent anti-aging activity, indicating that it can be used as an anti-aging drug candidate or as a phyto-cosmeceutical.
Suprapto Maat - One of the best experts on this subject based on the ideXlab platform.
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Anti-Aging Properties of Curcuma Heynena Valeton & Zipj: A Scientific Approach to its use in Javanese tradition
Elsevier B.V., 2018Co-Authors: Idha Kusumawati, Kresma Oky Kurniawan, Subhan Rullyansyah, Eka Pramyrtha Hestianah, Tri Anggono Prijo, Retno Widyowati, Juni Ekowati, Suprapto Maat, Katsuyoshi MatsunamiAbstract:Ethnopharmacological Relevance: Temu Giring, The Rhizome Of Curcuma Heyneana, is native to tropical regions, especially in Indonesia. It is traditionally used as a skin care, for cosmetic and body freshness, in Javanese and Balinese women, and has many other bioactivities such as antioxidant, anticancer and antiinflammatory. Aim of the study: The purpose of this study was to determine the antiaging activity of C. heyneana to prove its traditional use. Materials and methods: The antioxidant activity was determined using the DPPH free radical method, and anti- aging activity was examined using in vitro assays such as tyrosinase Inhibitor and Collagenase Inhibitor. In vivo tests were performed by observing histomorphologic changes in rat skin exposed to Ultraviolet (UV) rays. The total curcuminoid contents and chromatographic profiles were determined by Thin Layer Chromatography (TLC) – densitometry. Results: In all in vitro assays, all of the extracts showed a dose-dependent manner in the final concentration range from 62.5 to 500 µg/mL for DPPH assay and 31.25 to 250 µg/mL for tyrosinase inhibition and Collagenase inhibition assay. Curcuminoid (CUR), the active principle of Curcuma genus shows antioxidant, tyrosinase in- hibitor and Collagenase Inhibitor activity greater than all C. Heyneana extracts. The in vivo assay results showed that the topical application of the crude extract of C. Heyneana produced significant improvement effects on the UV-induced skin structure damage. The total CUR content was correlated with the anti-aging activity of Curcuma Heyneana. Conclusions: The results show that C. Heyneana contains antioxidant compounds and has potent anti-aging ac- tivity, indicating that it can be used as an anti-aging drug candidate or as a phyto-cosmeceutical. Keywords: Curcuma Anti aging Antioxidants, Collagenase Inhibitor Tyrosinase Inhibitor Rat skin histomorphologic change
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Anti-aging properties of Curcuma heyneana Valeton & Zipj: A scientific approach to its use in Javanese tradition.
Journal of ethnopharmacology, 2018Co-Authors: Idha Kusumawati, Kresma Oky Kurniawan, Subhan Rullyansyah, Eka Pramyrtha Hestianah, Tri Anggono Prijo, Retno Widyowati, Juni Ekowati, Suprapto Maat, Katsuyoshi MatsunamiAbstract:Abstract Ethnopharmacological relevance Temu giring, the rhizome of Curcuma heyneana Valeton & Zipj (C. heyneana), is native to tropical regions, especially in Indonesia. It is traditionally used as a skin care, for cosmetic and body freshness, in Javanese and Balinese women, and has many other bioactivities such as antioxidant, anticancer and antiinflammatory. Aim of the study The purpose of this study was to determine the antiaging activity of C. heyneana to prove its traditional use. Materials and methods The antioxidant activity was determined using the DPPH free radical method, and anti-aging activity was examined using in vitro assays such as tyrosinase Inhibitor and Collagenase Inhibitor. In vivo tests were performed by observing histomorphologic changes in rat skin exposed to Ultraviolet (UV) rays. The total curcuminoid contents and chromatographic profiles were determined by Thin Layer Chromatography (TLC) – densitometry. Results In all in vitro assays, all of the extracts showed a dose-dependent manner in the final concentration range from 62.5 to 500 µg/mL for DPPH assay and 31.25 to 250 µg/mL for tyrosinase inhibition and Collagenase inhibition assay. Curcuminoid (CUR), the active principle of Curcuma genus shows antioxidant, tyrosinase Inhibitor and Collagenase Inhibitor activity greater than all C. heyneana extracts. The in vivo assay results showed that the topical application of the crude extract of C. heyneana produced significant improvement effects on the UV-induced skin structure damage. The total CUR content was correlated with the anti-aging activity of Curcuma heyneana. Conclusions The results show that C. heyneana contains antioxidant compounds and has potent anti-aging activity, indicating that it can be used as an anti-aging drug candidate or as a phyto-cosmeceutical.