The Experts below are selected from a list of 54 Experts worldwide ranked by ideXlab platform
W B Van Leeuwen - One of the best experts on this subject based on the ideXlab platform.
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detection of Colon Flora in peritoneal drain fluid after colorectal surgery can rt pcr play a role in diagnosing anastomotic leakage
Iranian journal of microbiology, 2009Co-Authors: Niels Komen, M C Morsink, S Beiboer, A Miggelbrink, Paul Willemsen, E Van Der Harst, Johan F Lange, W B Van LeeuwenAbstract:Background and objectives: A semi-quantitative Real-Time PCR strategy was developed to identify indicator organisms for an indication on anastomotic leakage in peritoneal drainage fluid, Escherichia coli and Enterococcus faecalis. Materials and Methods: The analytical performance of the amplification method was validated with 10 culture-positive and 7 culture negative peritoneal drain fluid samples, obtained from 9 different patients with a colorectal anastomosis. Results: Real-Time PCR results were fully concordant with the microbiological culture results. However, among the culture negative samples, four false-positive RT-PCR results were found. All false-positives originated from a single patient with a surgical site infection. This may indicate an elevated sensitivity of the RT-PCR method. Conclusion: The results showed that the semi-quantitative RT-PCR method has the clear potential to be useful as a powerful tool in early detection of anastomotic leakage.
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detection of Colon Flora in peritoneal drain fluid after colorectal surgery can rt pcr play a role in diagnosing anastomotic leakage
Journal of Microbiological Methods, 2009Co-Authors: Niels Komen, M C Morsink, S Beiboer, A Miggelbrink, Paul Willemsen, E Van Der Harst, Johan F Lange, W B Van LeeuwenAbstract:A semi-quantitative Real-Time PCR strategy was developed to identify potential indicator organisms for anastomotic leakage in peritoneal drainage fluid, Escherichia coli and Enterococcus faecalis. The analytical performance of the amplification method was validated with 10 culture-positive and 7 culture-negative peritoneal drain fluid samples, obtained from 9 different patients with a colorectal anastomosis. Real-Time PCR results were fully concordant with the microbiological culture results. However, among the culture-negative samples, four false-positive RT-PCR results were found. All false-positives originated from a single patient with a surgical site infection. This may indicate an elevated sensitivity of the RT-PCR method. The results showed that the semi-quantitative RT-PCR method has a clear potential to be useful as a powerful tool in early detection of anastomotic leakage.
Niels Komen - One of the best experts on this subject based on the ideXlab platform.
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detection of Colon Flora in peritoneal drain fluid after colorectal surgery can rt pcr play a role in diagnosing anastomotic leakage
Iranian journal of microbiology, 2009Co-Authors: Niels Komen, M C Morsink, S Beiboer, A Miggelbrink, Paul Willemsen, E Van Der Harst, Johan F Lange, W B Van LeeuwenAbstract:Background and objectives: A semi-quantitative Real-Time PCR strategy was developed to identify indicator organisms for an indication on anastomotic leakage in peritoneal drainage fluid, Escherichia coli and Enterococcus faecalis. Materials and Methods: The analytical performance of the amplification method was validated with 10 culture-positive and 7 culture negative peritoneal drain fluid samples, obtained from 9 different patients with a colorectal anastomosis. Results: Real-Time PCR results were fully concordant with the microbiological culture results. However, among the culture negative samples, four false-positive RT-PCR results were found. All false-positives originated from a single patient with a surgical site infection. This may indicate an elevated sensitivity of the RT-PCR method. Conclusion: The results showed that the semi-quantitative RT-PCR method has the clear potential to be useful as a powerful tool in early detection of anastomotic leakage.
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detection of Colon Flora in peritoneal drain fluid after colorectal surgery can rt pcr play a role in diagnosing anastomotic leakage
Journal of Microbiological Methods, 2009Co-Authors: Niels Komen, M C Morsink, S Beiboer, A Miggelbrink, Paul Willemsen, E Van Der Harst, Johan F Lange, W B Van LeeuwenAbstract:A semi-quantitative Real-Time PCR strategy was developed to identify potential indicator organisms for anastomotic leakage in peritoneal drainage fluid, Escherichia coli and Enterococcus faecalis. The analytical performance of the amplification method was validated with 10 culture-positive and 7 culture-negative peritoneal drain fluid samples, obtained from 9 different patients with a colorectal anastomosis. Real-Time PCR results were fully concordant with the microbiological culture results. However, among the culture-negative samples, four false-positive RT-PCR results were found. All false-positives originated from a single patient with a surgical site infection. This may indicate an elevated sensitivity of the RT-PCR method. The results showed that the semi-quantitative RT-PCR method has a clear potential to be useful as a powerful tool in early detection of anastomotic leakage.
Johan F Lange - One of the best experts on this subject based on the ideXlab platform.
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detection of Colon Flora in peritoneal drain fluid after colorectal surgery can rt pcr play a role in diagnosing anastomotic leakage
Iranian journal of microbiology, 2009Co-Authors: Niels Komen, M C Morsink, S Beiboer, A Miggelbrink, Paul Willemsen, E Van Der Harst, Johan F Lange, W B Van LeeuwenAbstract:Background and objectives: A semi-quantitative Real-Time PCR strategy was developed to identify indicator organisms for an indication on anastomotic leakage in peritoneal drainage fluid, Escherichia coli and Enterococcus faecalis. Materials and Methods: The analytical performance of the amplification method was validated with 10 culture-positive and 7 culture negative peritoneal drain fluid samples, obtained from 9 different patients with a colorectal anastomosis. Results: Real-Time PCR results were fully concordant with the microbiological culture results. However, among the culture negative samples, four false-positive RT-PCR results were found. All false-positives originated from a single patient with a surgical site infection. This may indicate an elevated sensitivity of the RT-PCR method. Conclusion: The results showed that the semi-quantitative RT-PCR method has the clear potential to be useful as a powerful tool in early detection of anastomotic leakage.
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detection of Colon Flora in peritoneal drain fluid after colorectal surgery can rt pcr play a role in diagnosing anastomotic leakage
Journal of Microbiological Methods, 2009Co-Authors: Niels Komen, M C Morsink, S Beiboer, A Miggelbrink, Paul Willemsen, E Van Der Harst, Johan F Lange, W B Van LeeuwenAbstract:A semi-quantitative Real-Time PCR strategy was developed to identify potential indicator organisms for anastomotic leakage in peritoneal drainage fluid, Escherichia coli and Enterococcus faecalis. The analytical performance of the amplification method was validated with 10 culture-positive and 7 culture-negative peritoneal drain fluid samples, obtained from 9 different patients with a colorectal anastomosis. Real-Time PCR results were fully concordant with the microbiological culture results. However, among the culture-negative samples, four false-positive RT-PCR results were found. All false-positives originated from a single patient with a surgical site infection. This may indicate an elevated sensitivity of the RT-PCR method. The results showed that the semi-quantitative RT-PCR method has a clear potential to be useful as a powerful tool in early detection of anastomotic leakage.
E Van Der Harst - One of the best experts on this subject based on the ideXlab platform.
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detection of Colon Flora in peritoneal drain fluid after colorectal surgery can rt pcr play a role in diagnosing anastomotic leakage
Iranian journal of microbiology, 2009Co-Authors: Niels Komen, M C Morsink, S Beiboer, A Miggelbrink, Paul Willemsen, E Van Der Harst, Johan F Lange, W B Van LeeuwenAbstract:Background and objectives: A semi-quantitative Real-Time PCR strategy was developed to identify indicator organisms for an indication on anastomotic leakage in peritoneal drainage fluid, Escherichia coli and Enterococcus faecalis. Materials and Methods: The analytical performance of the amplification method was validated with 10 culture-positive and 7 culture negative peritoneal drain fluid samples, obtained from 9 different patients with a colorectal anastomosis. Results: Real-Time PCR results were fully concordant with the microbiological culture results. However, among the culture negative samples, four false-positive RT-PCR results were found. All false-positives originated from a single patient with a surgical site infection. This may indicate an elevated sensitivity of the RT-PCR method. Conclusion: The results showed that the semi-quantitative RT-PCR method has the clear potential to be useful as a powerful tool in early detection of anastomotic leakage.
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detection of Colon Flora in peritoneal drain fluid after colorectal surgery can rt pcr play a role in diagnosing anastomotic leakage
Journal of Microbiological Methods, 2009Co-Authors: Niels Komen, M C Morsink, S Beiboer, A Miggelbrink, Paul Willemsen, E Van Der Harst, Johan F Lange, W B Van LeeuwenAbstract:A semi-quantitative Real-Time PCR strategy was developed to identify potential indicator organisms for anastomotic leakage in peritoneal drainage fluid, Escherichia coli and Enterococcus faecalis. The analytical performance of the amplification method was validated with 10 culture-positive and 7 culture-negative peritoneal drain fluid samples, obtained from 9 different patients with a colorectal anastomosis. Real-Time PCR results were fully concordant with the microbiological culture results. However, among the culture-negative samples, four false-positive RT-PCR results were found. All false-positives originated from a single patient with a surgical site infection. This may indicate an elevated sensitivity of the RT-PCR method. The results showed that the semi-quantitative RT-PCR method has a clear potential to be useful as a powerful tool in early detection of anastomotic leakage.
Paul Willemsen - One of the best experts on this subject based on the ideXlab platform.
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detection of Colon Flora in peritoneal drain fluid after colorectal surgery can rt pcr play a role in diagnosing anastomotic leakage
Iranian journal of microbiology, 2009Co-Authors: Niels Komen, M C Morsink, S Beiboer, A Miggelbrink, Paul Willemsen, E Van Der Harst, Johan F Lange, W B Van LeeuwenAbstract:Background and objectives: A semi-quantitative Real-Time PCR strategy was developed to identify indicator organisms for an indication on anastomotic leakage in peritoneal drainage fluid, Escherichia coli and Enterococcus faecalis. Materials and Methods: The analytical performance of the amplification method was validated with 10 culture-positive and 7 culture negative peritoneal drain fluid samples, obtained from 9 different patients with a colorectal anastomosis. Results: Real-Time PCR results were fully concordant with the microbiological culture results. However, among the culture negative samples, four false-positive RT-PCR results were found. All false-positives originated from a single patient with a surgical site infection. This may indicate an elevated sensitivity of the RT-PCR method. Conclusion: The results showed that the semi-quantitative RT-PCR method has the clear potential to be useful as a powerful tool in early detection of anastomotic leakage.
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detection of Colon Flora in peritoneal drain fluid after colorectal surgery can rt pcr play a role in diagnosing anastomotic leakage
Journal of Microbiological Methods, 2009Co-Authors: Niels Komen, M C Morsink, S Beiboer, A Miggelbrink, Paul Willemsen, E Van Der Harst, Johan F Lange, W B Van LeeuwenAbstract:A semi-quantitative Real-Time PCR strategy was developed to identify potential indicator organisms for anastomotic leakage in peritoneal drainage fluid, Escherichia coli and Enterococcus faecalis. The analytical performance of the amplification method was validated with 10 culture-positive and 7 culture-negative peritoneal drain fluid samples, obtained from 9 different patients with a colorectal anastomosis. Real-Time PCR results were fully concordant with the microbiological culture results. However, among the culture-negative samples, four false-positive RT-PCR results were found. All false-positives originated from a single patient with a surgical site infection. This may indicate an elevated sensitivity of the RT-PCR method. The results showed that the semi-quantitative RT-PCR method has a clear potential to be useful as a powerful tool in early detection of anastomotic leakage.