The Experts below are selected from a list of 39546 Experts worldwide ranked by ideXlab platform
Lynn Vanhaecke - One of the best experts on this subject based on the ideXlab platform.
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untargeted metabolomics reveals elevated l carnitine metabolism in pig and rat Colon Tissue following red versus white meat intake
2021Co-Authors: Caroline Rombouts, Lieven Van Meulebroek, Margot De Spiegeleer, Sophie Goethals, Thomas Van Hecke, Stefaan De Smet, Winnok H De Vos, Lynn VanhaeckeAbstract:Scope The consumption of red and processed meat, and not white meat, has been associated with the development of various Western diseases such as colorectal cancer and type 2 diabetes. This work aimed at unravelling novel meat-associated mechanisms that are involved in disease development. Methods and results A non-hypothesis driven strategy of untargeted metabolomics was applied to assess Colon Tissue from rats (fed a high dose of beef versus white meat) and from pigs (fed red/processed meat versus white meat), receiving a realistic human background diet. An increased carnitine metabolism was observed, which was reflected by higher levels of acylcarnitines and 3-dehydroxycarnitine (rats and pigs) and trimethylamine-N-oxide (rats). While 3-dehydroxycarnitine was higher in HT29 cells, incubated with Colonic beef digests, acylcarnitine levels were reduced. This suggested an altered response from Colon cancer cell line towards meat-induced oxidative stress. Moreover, metabolic differences between rat and pigs were observed in N-glycolylneuraminic acid incorporation, prostaglandin and fatty acid synthesis. Conclusion This study demonstrated elevated (acyl)carnitine metabolism in Colon Tissue of animals that followed a red meat-based diet, providing mechanistic insights that may aid in explaining the nutritional-physiological correlation between red/processed meat and Western diseases. This article is protected by copyright. All rights reserved.
Winnok H De Vos - One of the best experts on this subject based on the ideXlab platform.
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untargeted metabolomics reveals elevated l carnitine metabolism in pig and rat Colon Tissue following red versus white meat intake
2021Co-Authors: Caroline Rombouts, Lieven Van Meulebroek, Margot De Spiegeleer, Sophie Goethals, Thomas Van Hecke, Stefaan De Smet, Winnok H De Vos, Lynn VanhaeckeAbstract:Scope The consumption of red and processed meat, and not white meat, has been associated with the development of various Western diseases such as colorectal cancer and type 2 diabetes. This work aimed at unravelling novel meat-associated mechanisms that are involved in disease development. Methods and results A non-hypothesis driven strategy of untargeted metabolomics was applied to assess Colon Tissue from rats (fed a high dose of beef versus white meat) and from pigs (fed red/processed meat versus white meat), receiving a realistic human background diet. An increased carnitine metabolism was observed, which was reflected by higher levels of acylcarnitines and 3-dehydroxycarnitine (rats and pigs) and trimethylamine-N-oxide (rats). While 3-dehydroxycarnitine was higher in HT29 cells, incubated with Colonic beef digests, acylcarnitine levels were reduced. This suggested an altered response from Colon cancer cell line towards meat-induced oxidative stress. Moreover, metabolic differences between rat and pigs were observed in N-glycolylneuraminic acid incorporation, prostaglandin and fatty acid synthesis. Conclusion This study demonstrated elevated (acyl)carnitine metabolism in Colon Tissue of animals that followed a red meat-based diet, providing mechanistic insights that may aid in explaining the nutritional-physiological correlation between red/processed meat and Western diseases. This article is protected by copyright. All rights reserved.
Caroline Rombouts - One of the best experts on this subject based on the ideXlab platform.
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untargeted metabolomics reveals elevated l carnitine metabolism in pig and rat Colon Tissue following red versus white meat intake
2021Co-Authors: Caroline Rombouts, Lieven Van Meulebroek, Margot De Spiegeleer, Sophie Goethals, Thomas Van Hecke, Stefaan De Smet, Winnok H De Vos, Lynn VanhaeckeAbstract:Scope The consumption of red and processed meat, and not white meat, has been associated with the development of various Western diseases such as colorectal cancer and type 2 diabetes. This work aimed at unravelling novel meat-associated mechanisms that are involved in disease development. Methods and results A non-hypothesis driven strategy of untargeted metabolomics was applied to assess Colon Tissue from rats (fed a high dose of beef versus white meat) and from pigs (fed red/processed meat versus white meat), receiving a realistic human background diet. An increased carnitine metabolism was observed, which was reflected by higher levels of acylcarnitines and 3-dehydroxycarnitine (rats and pigs) and trimethylamine-N-oxide (rats). While 3-dehydroxycarnitine was higher in HT29 cells, incubated with Colonic beef digests, acylcarnitine levels were reduced. This suggested an altered response from Colon cancer cell line towards meat-induced oxidative stress. Moreover, metabolic differences between rat and pigs were observed in N-glycolylneuraminic acid incorporation, prostaglandin and fatty acid synthesis. Conclusion This study demonstrated elevated (acyl)carnitine metabolism in Colon Tissue of animals that followed a red meat-based diet, providing mechanistic insights that may aid in explaining the nutritional-physiological correlation between red/processed meat and Western diseases. This article is protected by copyright. All rights reserved.
Cornelia M Ulrich - One of the best experts on this subject based on the ideXlab platform.
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genotype based gene expression in Colon Tissue prediction accuracy and relationship with the prognosis of colorectal cancer patients
2020Co-Authors: Heike Deutelmoser, Justo Lorenzo Bermejo, Axel Benner, Korbinian Weigl, Hanla A Park, Mariam Haffa, Esther Herpel, Martin Schneider, Cornelia M UlrichAbstract:Colorectal cancer (CRC) survival has environmental and inherited components. The expression of specific genes can be inferred based on individual genotypes-so called expression quantitative trait loci. In this study, we used the PrediXcan method to predict gene expression in normal Colon Tissue using individual genotype data from 91 CRC patients and examined the correlation ρ between predicted and measured gene expression levels. Out of 5434 predicted genes, 58% showed a negative ρ value and only 16% presented a ρ higher than 0.10. We subsequently investigated the association between genotype-based gene expression in Colon Tissue for genes with ρ > 0.10 and survival of 4436 CRC patients. We identified an inverse association between the predicted expression of ARID3B and CRC-specific survival for patients with a body mass index greater than or equal to 30 kg/m2 (HR (hazard ratio) = 0.66 for an expression higher vs. lower than the median, p = 0.005). This association was validated using genotype and clinical data from the UK Biobank (HR = 0.74, p = 0.04). In addition to the identification of ARID3B expression in normal Colon Tissue as a candidate prognostic biomarker for obese CRC patients, our study illustrates the challenges of genotype-based prediction of gene expression, and the advantage of reassessing the prediction accuracy in a subset of the study population using measured gene expression data.
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senescence associated Tissue microenvironment promotes Colon cancer formation through the secretory factor gdf15
2019Co-Authors: Yuna Guo, Cornelia M Ulrich, Jessica L Ayers, Kelly T Carter, Ting Wang, Sean K Maden, Darwin Edmond, Polly Newcomb P, William M GradyAbstract:The risk of colorectal cancer (CRC) varies between people, and the cellular mechanisms mediating the differences in risk are largely unknown. Senescence has been implicated as a causative cellular mechanism for many diseases, including cancer, and may affect the risk for CRC. Senescent fibroblasts that accumulate in Tissues secondary to aging and oxidative stress have been shown to promote cancer formation via a senescence-associated secretory phenotype (SASP). In this study, we assessed the role of senescence and the SASP in CRC formation. Using primary human Colon Tissue, we found an accumulation of senescent fibroblasts in normal Tissues from individuals with advanced adenomas or carcinomas in comparison with individuals with no polyps or CRC. In in vitro and ex vivo model systems, we induced senescence using oxidative stress in Colon fibroblasts and demonstrated that the senescent fibroblasts secrete GDF15 as an essential SASP factor that promotes cell proliferation, migration, and invasion in Colon adenoma and CRC cell lines as well as primary Colon organoids via the MAPK and PI3K signaling pathways. In addition, we observed increased mRNA expression of GDF15 in primary normal Colon Tissue from people at increased risk for CRC in comparison with average risk individuals. These findings implicate the importance of a senescence-associated Tissue microenvironment and the secretory factor GDF15 in promoting CRC formation.
Stefaan De Smet - One of the best experts on this subject based on the ideXlab platform.
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untargeted metabolomics reveals elevated l carnitine metabolism in pig and rat Colon Tissue following red versus white meat intake
2021Co-Authors: Caroline Rombouts, Lieven Van Meulebroek, Margot De Spiegeleer, Sophie Goethals, Thomas Van Hecke, Stefaan De Smet, Winnok H De Vos, Lynn VanhaeckeAbstract:Scope The consumption of red and processed meat, and not white meat, has been associated with the development of various Western diseases such as colorectal cancer and type 2 diabetes. This work aimed at unravelling novel meat-associated mechanisms that are involved in disease development. Methods and results A non-hypothesis driven strategy of untargeted metabolomics was applied to assess Colon Tissue from rats (fed a high dose of beef versus white meat) and from pigs (fed red/processed meat versus white meat), receiving a realistic human background diet. An increased carnitine metabolism was observed, which was reflected by higher levels of acylcarnitines and 3-dehydroxycarnitine (rats and pigs) and trimethylamine-N-oxide (rats). While 3-dehydroxycarnitine was higher in HT29 cells, incubated with Colonic beef digests, acylcarnitine levels were reduced. This suggested an altered response from Colon cancer cell line towards meat-induced oxidative stress. Moreover, metabolic differences between rat and pigs were observed in N-glycolylneuraminic acid incorporation, prostaglandin and fatty acid synthesis. Conclusion This study demonstrated elevated (acyl)carnitine metabolism in Colon Tissue of animals that followed a red meat-based diet, providing mechanistic insights that may aid in explaining the nutritional-physiological correlation between red/processed meat and Western diseases. This article is protected by copyright. All rights reserved.