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Sylvaine Camous - One of the best experts on this subject based on the ideXlab platform.
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maternal nutrition during pregnancy affects testicular and bone development glucose metabolism and response to overnutrition in weaned horses up to two years
PLOS ONE, 2017Co-Authors: Morgane Robles, Camille Gautier, Luis Mendoza, Pauline Peugnet, Cédric Dubois, Michèle Dahirel, Jean-philippe Lejeune, Isabelle Caudron, Isabelle Guenon, Sylvaine CamousAbstract:Introduction Pregnant mares and post-weaning foals are often fed concentrates rich in soluble carbohydrates, together with forage. Recent studies suggest that the use of concentrates is linked to alterations of metabolism and the development of osteochondrosis in foals. The aim of this study was to determine if broodmare diet during gestation affects metabolism, osteoarticular status and growth of yearlings overfed from 20 to 24 months of age and/or sexual maturity in prepubertal Colts. Material and methods Twenty-four saddlebred mares were fed forage only (n = 12, group F) or cracked barley and forage (n = 12, group B) from mid-gestation until foaling. Colts were gelded at 12 months of age. Between 20 and 24 months of age, all yearlings were overfed (+140% of requirements) using an automatic concentrate feeder. Offspring were monitored for growth between 6 and 24 months of age, glucose homeostasis was evaluated via modified frequently sampled intra veinous glucose tolerance test (FSIGT) at 19 and 24 months of age and osteoarticular status was investigated using radiographic examinations at 24 months of age. The structure and function of testicles from prepubertal Colts were analyzed using stereology and RT-qPCR. Results Post-weaning weight growth was not different between groups. Testicular maturation was delayed in F Colts compared to B Colts at 12 months of age. From 19 months of age, the cannon bone was wider in B vs F yearlings. F yearlings were more insulin resistant at 19 months compared to B yearlings but B yearlings were affected more severely by overnutrition with reduced insulin sensitivity. The osteoarticular status at 24 months of age was not different between groups. Conclusion In conclusion, nutritional management of the pregnant broodmare and the growing foal may affect sexual maturity of Colts and the metabolism of foals until 24 months of age. These effects may be deleterious for reproductive and sportive performances in older horses.
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Maternal nutrition during pregnancy affects testicular and bone development, glucose metabolism and response to overnutrition in weaned horses up to two years
PLoS ONE, 2017Co-Authors: Morgane Robles, Camille Gautier, Luis Mendoza, Pauline Peugnet, Cédric Dubois, Michèle Dahirel, Jean-philippe Lejeune, Isabelle Caudron, Isabelle Guenon, Sylvaine CamousAbstract:Pregnant mares and post-weaning foals are often fed concentrates rich in soluble carbohydrates, together with forage. Recent studies suggest that the use of concentrates is linked to alterations of metabolism and the development of osteochondrosis in foals. The aim of this study was to determine if broodmare diet during gestation affects metabolism, osteoarticular status and growth of yearlings overfed from 20 to 24 months of age and/or sexual maturity in prepubertal Colts. Twenty-four saddlebred mares were fed forage only (n = 12, group F) or cracked barley and forage (n = 12, group B) from mid-gestation until foaling. Colts were gelded at 12 months of age. Between 20 and 24 months of age, all yearlings were overfed (+140% of requirements) using an automatic concentrate feeder. Offspring were monitored for growth between 6 and 24 months of age, glucose homeostasis was evaluated via modified frequently sampled intra veinous glucose tolerance test (FSIGT) at 19 and 24 months of age and osteoarticular status was investigated using radiographic examinations at 24 months of age. The structure and function of testicles from prepubertal Colts were analyzed using stereology and RT-qPCR. Post-weaning weight growth was not different between groups. Testicular maturation was delayed in F Colts compared to B Colts at 12 months of age. From 19 months of age, the cannon bone was wider in B vs F yearlings. F yearlings were more insulin resistant at 19 months compared to B yearlings but B yearlings were affected more severely by overnutrition with reduced insulin sensitivity. The osteoarticular status at 24 months of age was not different between groups. In conclusion, nutritional management of the pregnant broodmare and the growing foal may affect sexual maturity of Colts and the metabolism of foals until 24 months of age. These effects may be deleterious for reproductive and sportive performances in older horses.
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Primers sequences used for amplification of equine products in RT-qPCR assays in testicles of prepubertal Colts.
2017Co-Authors: Morgane Robles, Camille Gautier, Luis Mendoza, Pauline Peugnet, Cédric Dubois, Michèle Dahirel, Jean-philippe Lejeune, Isabelle Caudron, Isabelle Guenon, Sylvaine CamousAbstract:Primers sequences used for amplification of equine products in RT-qPCR assays in testicles of prepubertal Colts.
Franci Demsar - One of the best experts on this subject based on the ideXlab platform.
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dependence of blood clot lysis on the mode of transport of urokinase into the clot a magnetic resonance imaging study in vitro
Thrombosis and Haemostasis, 1991Co-Authors: Aleš Blinc, Gorazd Planinsic, Orest Jarh, Gojmir Lahajnar, Aleksander Zidanšek, Dušan Keber, Franci DemsarAbstract:: Magnetic resonance imaging was employed to study the dependence of clot lysing patterns on two different modes of transport of urokinase into whole blood clots. In one group of clots (nonperfused clots, n1 = 10), access of urokinase to the fibrin network was possible by diffusion only, whereas in the other group (perfused clots, n2 = 10) bulk flow of plasma containing urokinase was instituted through occlusive clots by a pressure difference of 3.7 kPa (37 cm H2O) across 3 cm long clots with a diameter of 4 mm. It was determined separately that this pressure difference resulted in a volume flow rate of 5.05 +/- 2.4 x 10(-2) ml/min through occlusive clots. Perfused clots diminished in size significantly in comparison to nonperfused ones already after 20 min (p less than 0.005). Linear regression analysis of two-dimensional clot sizes measured by MRI showed that the rate of lysis was more than 50-times faster in the perfused group in comparison to the nonperfused group. It was concluded that penetration of the thrombolytic agent into clots by perfusion is much more effective than by diffusion. Our results might have some implications for understanding the differences in lysis of arterial and venous thrombi.
Wrana Aleksander - One of the best experts on this subject based on the ideXlab platform.
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Laboratory pull-out tests on fully grouted rock bolts and cable bolts: results and lessons learned
Journal of Rock Mechanics and Geotechnical Engineering, 2017Co-Authors: Isabelle Thenevin, Laura Blanco Martin, Faouzi Hadj Hassen, Jacques Schleifer, Zbigniew Lubosik, Wrana AleksanderAbstract:Laboratory pull-out tests were conducted on the following rock bolts and cable bolts: steel rebars, smooth steel bars, fiberglass reinforced polymer threaded bolts, flexible cable bolts, IR5/IN special cable bolts and Mini-cage cable bolts. The diameter of the tested bolts was between 16 mm and 26 mm. The bolts were grouted in a sandstone sample using resin or cement grouts. The tests were conducted under either constant radial stiffness or constant confining pressure boundary conditions applied on the outer surface of the rock sample. In most tests, the rate of displacement was about 0.02 mm/s. The tests were performed using a pull-out bench that allows testing a wide range of parameters. This paper provides an extensive database of laboratory pull-out test results and confirms the influence of the confining pressure and the embedment length on the pull-out response (rock bolts and cable bolts). It also highlights the sensitivity of the results to the operating conditions and to the behaviour of the sample as a whole, which cannot be neglected when the test results are used to assess the bolt-grout or the grout-rock interface.
Songfeng Li - One of the best experts on this subject based on the ideXlab platform.
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itraq based proteomics investigation of aqueous humor from patients with coats disease
PLOS ONE, 2016Co-Authors: Qiong Yang, Xudong Song, Hai Lu, Songfeng LiAbstract:BACKGROUND: Coats' disease is an uncommon form of retinal telangiectasis, and the identification of novel proteins that contribute to the development of Coats' disease is useful for improving treatment efficacy. Proteomic techniques have been used to study many eye diseases; however, few studies have used proteomics to study the development of Coats' disease. METHODS: Isobaric tagging for relative and absolute protein quantification (iTRAQ) was employed to screen differentially expressed proteins (DEPs) in the aqueous humor (AH) between stage 3A patients (n = 8), stage 3B patients (n = 14), stage 4 patients (n = 2) and control patients (n = 20). Differentially co-expressed proteins (DCPs) were present in all three stages of Coats' disease and were considered disease-specific proteins. These proteins were further analyzed using Gene Ontology (GO) functional annotations. RESULTS: A total of 819 proteins were identified in the AH, 222 of which were significantly differentially expressed (fold change > 2 and P < 0.05) in the samples from at least one stage of Coats' disease. Of the DEPs, 46 were found among all three stages of Coats' disease and the controls; therefore, they were considered Coats' disease-specific proteins (DCPs). A GO classification analysis indicated that the DCPs were closely related to structural molecule activity, cell adhesion molecule binding and receptor binding. Western blotting confirmed the expression levels of haptoglobin and apolipoprotein C-I were significantly up-regulated in Coats' disease. CONCLUSIONS: The 46 Coats' disease-specific proteins may provide additional insights into the mechanism of Coats' disease and represent potential biomarkers for identifying individuals with Coats' disease.
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itraq based proteomics investigation of aqueous humor from patients with coats disease
PLOS ONE, 2016Co-Authors: Qiong Yang, Xudong Song, Hai Lu, Songfeng LiAbstract:BACKGROUND: Coats' disease is an uncommon form of retinal telangiectasis, and the identification of novel proteins that contribute to the development of Coats' disease is useful for improving treatment efficacy. Proteomic techniques have been used to study many eye diseases; however, few studies have used proteomics to study the development of Coats' disease. METHODS: Isobaric tagging for relative and absolute protein quantification (iTRAQ) was employed to screen differentially expressed proteins (DEPs) in the aqueous humor (AH) between stage 3A patients (n = 8), stage 3B patients (n = 14), stage 4 patients (n = 2) and control patients (n = 20). Differentially co-expressed proteins (DCPs) were present in all three stages of Coats' disease and were considered disease-specific proteins. These proteins were further analyzed using Gene Ontology (GO) functional annotations. RESULTS: A total of 819 proteins were identified in the AH, 222 of which were significantly differentially expressed (fold change > 2 and P < 0.05) in the samples from at least one stage of Coats' disease. Of the DEPs, 46 were found among all three stages of Coats' disease and the controls; therefore, they were considered Coats' disease-specific proteins (DCPs). A GO classification analysis indicated that the DCPs were closely related to structural molecule activity, cell adhesion molecule binding and receptor binding. Western blotting confirmed the expression levels of haptoglobin and apolipoprotein C-I were significantly up-regulated in Coats' disease. CONCLUSIONS: The 46 Coats' disease-specific proteins may provide additional insights into the mechanism of Coats' disease and represent potential biomarkers for identifying individuals with Coats' disease.
Morgane Robles - One of the best experts on this subject based on the ideXlab platform.
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maternal nutrition during pregnancy affects testicular and bone development glucose metabolism and response to overnutrition in weaned horses up to two years
PLOS ONE, 2017Co-Authors: Morgane Robles, Camille Gautier, Luis Mendoza, Pauline Peugnet, Cédric Dubois, Michèle Dahirel, Jean-philippe Lejeune, Isabelle Caudron, Isabelle Guenon, Sylvaine CamousAbstract:Introduction Pregnant mares and post-weaning foals are often fed concentrates rich in soluble carbohydrates, together with forage. Recent studies suggest that the use of concentrates is linked to alterations of metabolism and the development of osteochondrosis in foals. The aim of this study was to determine if broodmare diet during gestation affects metabolism, osteoarticular status and growth of yearlings overfed from 20 to 24 months of age and/or sexual maturity in prepubertal Colts. Material and methods Twenty-four saddlebred mares were fed forage only (n = 12, group F) or cracked barley and forage (n = 12, group B) from mid-gestation until foaling. Colts were gelded at 12 months of age. Between 20 and 24 months of age, all yearlings were overfed (+140% of requirements) using an automatic concentrate feeder. Offspring were monitored for growth between 6 and 24 months of age, glucose homeostasis was evaluated via modified frequently sampled intra veinous glucose tolerance test (FSIGT) at 19 and 24 months of age and osteoarticular status was investigated using radiographic examinations at 24 months of age. The structure and function of testicles from prepubertal Colts were analyzed using stereology and RT-qPCR. Results Post-weaning weight growth was not different between groups. Testicular maturation was delayed in F Colts compared to B Colts at 12 months of age. From 19 months of age, the cannon bone was wider in B vs F yearlings. F yearlings were more insulin resistant at 19 months compared to B yearlings but B yearlings were affected more severely by overnutrition with reduced insulin sensitivity. The osteoarticular status at 24 months of age was not different between groups. Conclusion In conclusion, nutritional management of the pregnant broodmare and the growing foal may affect sexual maturity of Colts and the metabolism of foals until 24 months of age. These effects may be deleterious for reproductive and sportive performances in older horses.
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Maternal nutrition during pregnancy affects testicular and bone development, glucose metabolism and response to overnutrition in weaned horses up to two years
PLoS ONE, 2017Co-Authors: Morgane Robles, Camille Gautier, Luis Mendoza, Pauline Peugnet, Cédric Dubois, Michèle Dahirel, Jean-philippe Lejeune, Isabelle Caudron, Isabelle Guenon, Sylvaine CamousAbstract:Pregnant mares and post-weaning foals are often fed concentrates rich in soluble carbohydrates, together with forage. Recent studies suggest that the use of concentrates is linked to alterations of metabolism and the development of osteochondrosis in foals. The aim of this study was to determine if broodmare diet during gestation affects metabolism, osteoarticular status and growth of yearlings overfed from 20 to 24 months of age and/or sexual maturity in prepubertal Colts. Twenty-four saddlebred mares were fed forage only (n = 12, group F) or cracked barley and forage (n = 12, group B) from mid-gestation until foaling. Colts were gelded at 12 months of age. Between 20 and 24 months of age, all yearlings were overfed (+140% of requirements) using an automatic concentrate feeder. Offspring were monitored for growth between 6 and 24 months of age, glucose homeostasis was evaluated via modified frequently sampled intra veinous glucose tolerance test (FSIGT) at 19 and 24 months of age and osteoarticular status was investigated using radiographic examinations at 24 months of age. The structure and function of testicles from prepubertal Colts were analyzed using stereology and RT-qPCR. Post-weaning weight growth was not different between groups. Testicular maturation was delayed in F Colts compared to B Colts at 12 months of age. From 19 months of age, the cannon bone was wider in B vs F yearlings. F yearlings were more insulin resistant at 19 months compared to B yearlings but B yearlings were affected more severely by overnutrition with reduced insulin sensitivity. The osteoarticular status at 24 months of age was not different between groups. In conclusion, nutritional management of the pregnant broodmare and the growing foal may affect sexual maturity of Colts and the metabolism of foals until 24 months of age. These effects may be deleterious for reproductive and sportive performances in older horses.
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Primers sequences used for amplification of equine products in RT-qPCR assays in testicles of prepubertal Colts.
2017Co-Authors: Morgane Robles, Camille Gautier, Luis Mendoza, Pauline Peugnet, Cédric Dubois, Michèle Dahirel, Jean-philippe Lejeune, Isabelle Caudron, Isabelle Guenon, Sylvaine CamousAbstract:Primers sequences used for amplification of equine products in RT-qPCR assays in testicles of prepubertal Colts.