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Reiner Babiel - One of the best experts on this subject based on the ideXlab platform.

  • evaluation of an upgraded version of the roche cobas ampliprep cobas taqman hiv 1 test for hiv 1 load quantification
    Journal of Clinical Microbiology, 2010
    Co-Authors: Florence Damond, Veronique Avettandfenoel, Gilles Collin, Benedicte Roquebert, Jeanchristophe Plantier, A Ganon, Dorothea Sizmann, Reiner Babiel, Joachim Glaubitz, Marielaure Chaix
    Abstract:

    We evaluated the performance of the prototype Cobas AmpliPrep/Cobas TaqMan HIV-1 test, version 2.0, using prospective and archived clinical samples initially underquantitated by the Cobas AmpliPrep/Cobas TaqMan HIV-1 test. The performance of the new test was significantly improved, and the majority of the underquantitation observed with the first-version test was eliminated.

  • fully automated quantification of human immunodeficiency virus hiv type 1 rna in human plasma by the cobas ampliprep cobas taqman system
    Journal of Clinical Virology, 2007
    Co-Authors: Wolfram Schumacher, Edmund Frick, Mirko Kauselmann, Viola Maierhoyle, Reinier Van Der Vliet, Reiner Babiel
    Abstract:

    Abstract Background HIV-1 RNA is a key parameter for reliable diagnosis and treatment of HIV-1 infection. The determination of HIV-1 RNA reduces the pre-seroconversion period in the diagnosis of HIV-1 infection and supports clinical management of HIV-1-infected patients. Objectives and study design The COBAS ® AmpliPrep/COBAS ® TaqMan ® HIV-1 Test combines automated extraction of total nucleic acids on the COBAS ® AmpliPrep Instrument with real-time PCR on the COBAS ® TaqMan ® Analyzer, thus greatly reducing hands-on time during sample preparation and amplification/detection. The test was evaluated for sensitivity, dynamic range, precision, subtype inclusivity, interfering substances, diagnostic and analytical specificity, as well as correlation with three other commercial tests for HIV-1 RNA quantification. Results The COBAS ® AmpliPrep/COBAS ® TaqMan ® HIV-1 Test demonstrated an assay sensitivity of 40 copies/mL, a greater than 5 log 10 measuring range of 40–1.0E+07 copies/mL (1.6–7.0 log 10 ) and a reliable determination of HIV-1 group M and N subtypes in EDTA plasma. Quantification results were highly correlated with those obtained by the COBAS ® AMPLICOR ® HIV-1 MONITOR Test v1.5, the COBAS ® AmpliPrep/COBAS ® AMPLICOR ® HIV-1 MONITOR Test v1.5 and the VERSANT ® HIV-1 RNA 3.0 assay. Conclusions The COBAS ® AmpliPrep/COBAS ® TaqMan ® HIV-1 Test excellently satisfies the requirements for reliable quantification of HIV-1 RNA in clinical specimens by a broad linear measuring range and a fully automated quantification procedure. It is highly appropriate for therapy monitoring and routine management of HIV-1 infection.

  • fully automated quantification of hepatitis c virus hcv rna in human plasma and human serum by the cobas ampliprep cobas taqman system
    Journal of Clinical Virology, 2007
    Co-Authors: Dorothea Sizmann, Claudia Boeck, Juergen Boelter, Daniela Fischer, Marion Miethke, Stefan Nicolaus, Markus Zadak, Reiner Babiel
    Abstract:

    Abstract Background HCV RNA is commonly recognized as key parameter for reliable diagnosis and treatment monitoring of HCV infection. Determination of blood HCV RNA concentrations reduces the pre-seroconversion period in the diagnosis of HCV infection and supports management of interferon alpha-based therapies of chronic HCV infection. Objectives and study design The COBAS ® AmpliPrep/COBAS ® TaqMan ® HCV Test combines automated extraction of nucleic acids on the COBAS ® AmpliPrep Instrument with real-time PCR on the COBAS ® TaqMan ® Analyzer, thus greatly reducing hands-on time during sample preparation and amplification/detection. The test, which is calibrated to the 1st International HCV WHO Standard, was evaluated for sensitivity, dynamic range, precision, matrix equivalence, genotype inclusivity, interfering substances, diagnostic and analytical specificity, as well as for correlation with two other commercial tests for HCV RNA quantification. Results The COBAS ® AmpliPrep/COBAS ® TaqMan ® HCV Test demonstrated a >6-log dynamic range of 43–6.90E+7 IU/mL, a sensitivity (95% hit rate) of at least 15 IU/mL for HCV WHO Standard and a comparable quantification of genotypes 1–6. HCV quantification results were in good correlation with those obtained by the COBAS ® AMPLICOR ® HCV MONITOR Test v2.0 and the VERSANT ® HCV RNA 3.0 test. Conclusions The fully automated COBAS ® AmpliPrep/COBAS ® TaqMan ® HCV Test excellently accomplishes the requirements for highly sensitive detection and reliable quantification of HCV in clinical samples and thus improves therapy monitoring and management of HCV infection.

Dorothea Sizmann - One of the best experts on this subject based on the ideXlab platform.

  • evaluation of an upgraded version of the roche cobas ampliprep cobas taqman hiv 1 test for hiv 1 load quantification
    Journal of Clinical Microbiology, 2010
    Co-Authors: Florence Damond, Veronique Avettandfenoel, Gilles Collin, Benedicte Roquebert, Jeanchristophe Plantier, A Ganon, Dorothea Sizmann, Reiner Babiel, Joachim Glaubitz, Marielaure Chaix
    Abstract:

    We evaluated the performance of the prototype Cobas AmpliPrep/Cobas TaqMan HIV-1 test, version 2.0, using prospective and archived clinical samples initially underquantitated by the Cobas AmpliPrep/Cobas TaqMan HIV-1 test. The performance of the new test was significantly improved, and the majority of the underquantitation observed with the first-version test was eliminated.

  • fully automated quantification of hepatitis c virus hcv rna in human plasma and human serum by the cobas ampliprep cobas taqman system
    Journal of Clinical Virology, 2007
    Co-Authors: Dorothea Sizmann, Claudia Boeck, Juergen Boelter, Daniela Fischer, Marion Miethke, Stefan Nicolaus, Markus Zadak, Reiner Babiel
    Abstract:

    Abstract Background HCV RNA is commonly recognized as key parameter for reliable diagnosis and treatment monitoring of HCV infection. Determination of blood HCV RNA concentrations reduces the pre-seroconversion period in the diagnosis of HCV infection and supports management of interferon alpha-based therapies of chronic HCV infection. Objectives and study design The COBAS ® AmpliPrep/COBAS ® TaqMan ® HCV Test combines automated extraction of nucleic acids on the COBAS ® AmpliPrep Instrument with real-time PCR on the COBAS ® TaqMan ® Analyzer, thus greatly reducing hands-on time during sample preparation and amplification/detection. The test, which is calibrated to the 1st International HCV WHO Standard, was evaluated for sensitivity, dynamic range, precision, matrix equivalence, genotype inclusivity, interfering substances, diagnostic and analytical specificity, as well as for correlation with two other commercial tests for HCV RNA quantification. Results The COBAS ® AmpliPrep/COBAS ® TaqMan ® HCV Test demonstrated a >6-log dynamic range of 43–6.90E+7 IU/mL, a sensitivity (95% hit rate) of at least 15 IU/mL for HCV WHO Standard and a comparable quantification of genotypes 1–6. HCV quantification results were in good correlation with those obtained by the COBAS ® AMPLICOR ® HCV MONITOR Test v2.0 and the VERSANT ® HCV RNA 3.0 test. Conclusions The fully automated COBAS ® AmpliPrep/COBAS ® TaqMan ® HCV Test excellently accomplishes the requirements for highly sensitive detection and reliable quantification of HCV in clinical samples and thus improves therapy monitoring and management of HCV infection.

Marielaure Chaix - One of the best experts on this subject based on the ideXlab platform.

Daniel Cook - One of the best experts on this subject based on the ideXlab platform.

  • The Effect of Co-Administration of Death Camas (Zigadenus spp.) and Low Larkspur (Delphinium spp.) in Cattle
    Toxins, 2016
    Co-Authors: Kevin D. Welch, Dale R. Gardner, Clinton A. Stonecipher, Benedict T. Green, James A. Pfister, Daniel Cook
    Abstract:

    In many rangeland settings, there is more than one potential poisonous plant. Two poisonous plants that are often found growing simultaneously in the same location in North American rangelands are death camas (Zigadenus spp.) and low larkspur (Delphinium spp.). The objective of this study was to determine if co-administration of death camas would exacerbate the toxicity of low larkspur in cattle. Cattle dosed with 2.0 g of death camas/kg BW showed slight frothing and lethargy, whereas cattle dosed with both death camas and low larkspur showed increased clinical signs of poisoning. Although qualitative differences in clinical signs of intoxication in cattle co-treated with death camas and low larkspur were observed, there were not any significant quantitative differences in heart rate or exercise-induced muscle fatigue. Co-treatment with death camas and low larkspur did not affect the serum zygacine kinetics, however, there was a difference in the larkspur alkaloid kinetics in the co-exposure group. Overall, the results from this study suggest that co-exposure to death camas and low larkspur is not significantly more toxic to cattle than exposure to the plants individually. The results from this study increase our knowledge and understanding regarding the acute toxicity of death camas and low larkspur in cattle.

  • The effect of low larkspur (Delphinium spp.) co-administration on the acute toxicity of death camas (Zigadenus spp.) in sheep.
    Toxicon : official journal of the International Society on Toxinology, 2013
    Co-Authors: Dale R. Gardner, Clinton A. Stonecipher, James A. Pfister, Kip E. Panter, Daniel Cook
    Abstract:

    In most cases where livestock are poisoned by plants in a range setting, there is more than one potential poisonous plant in the same area. Two poisonous plants that are often found growing simultaneously in the same location are death camas (Zigadenus spp.) and low larkspur (Delphinium spp.). Sheep are known to be susceptible to death camas poisoning while they are thought to be resistant to larkspur. The objective of this study was to determine if co-administration of low larkspur would exacerbate the toxicity of death camas in sheep. A dose finding study was performed to find a dose of death camas that caused minimal clinical signs of poisoning. Sheep were observed for clinical signs of poisoning as well as changes in heart rate and muscle fatigue. Sheep dosed with 1.14 g of death camas per kg BW showed slight frothing and lethargy, whereas sheep dosed with death camas and low larkspur showed slightly more noticeable clinical signs of poisoning. Sheep dosed with only low larkspur, at 7.8 g/kg BW, showed no signs of poisoning. Although we observed a qualitative difference in clinical signs of intoxication in sheep co-treated with death camas and low larkspur we did not detect any quantitative differences in heart rate, exercise-induced muscle fatigue, or differences in serum zygacine kinetics. Consequently, the results from this study suggest that low larkspur does not affect the toxicity of death camas in sheep. The results from this study increase knowledge and understanding regarding the acute toxicity of death camas and low larkspur in sheep. As combined intoxications are most likely common, this information will be useful in further developing management recommendations for ranchers and in designing additional experiments to study the toxicity of death camas to other livestock species.

J De Keyser - One of the best experts on this subject based on the ideXlab platform.

  • abundant molecular oxygen in the coma of comet 67p churyumov gerasimenko
    Nature, 2015
    Co-Authors: Andre Bieler, Kathrin Altwegg, H Balsiger, A Barnun, Jeanjacques Berthelier, P Bochsler, Christelle Briois, Ursina Calmonte, Michael R Combi, J De Keyser
    Abstract:

    The composition of the neutral gas Comas of most comets is dominated by H2O,CO and CO2, typically comprising as much as 95 per cent of the total gas density1. In addition, cometary Comas have been found to contain a rich array of other molecules, including sulfuric compounds and complex hydrocarbons. Molecular oxygen (O2), however, despite its detection on other icy bodies such as the moons of Jupiter and Saturn2,3, has remained undetected in cometary Comas. Here we report in situ measurement of O2 in the coma of comet 67P/Churyumov–Gerasimenko, with local abundances ranging from one per cent to ten per cent relative to H2O and with a mean value of 3.8060.85 per cent. Our observations indicate that the O2/H2O ratio is isotropic in the coma and does not change systematically with heliocentric distance. This suggests that primordial O2 was incorporated into the nucleus during the comet’s formation, which is unexpected given the low upper limits from remote sensing observations4. Current Solar System formation models do not predict conditions that would allow this to occur.

  • Abundant molecular oxygen in the coma of comet 67P/Churyumov–Gerasimenko
    Nature, 2015
    Co-Authors: Andre Bieler, Kathrin Altwegg, H Balsiger, Jeanjacques Berthelier, P Bochsler, Christelle Briois, Ursina Calmonte, Michael R Combi, Akiva Bar-nun, J De Keyser
    Abstract:

    The composition of the neutral gas Comas of most comets is dominated by H2O,CO and CO2, typically comprising as much as 95 per cent of the total gas density1. In addition, cometary Comas have been found to contain a rich array of other molecules, including sulfuric compounds and complex hydrocarbons. Molecular oxygen (O2), however, despite its detection on other icy bodies such as the moons of Jupiter and Saturn2,3, has remained undetected in cometary Comas. Here we report in situ measurement of O2 in the coma of comet 67P/Churyumov–Gerasimenko, with local abundances ranging from one per cent to ten per cent relative to H2O and with a mean value of 3.8060.85 per cent. Our observations indicate that the O2/H2O ratio is isotropic in the coma and does not change systematically with heliocentric distance. This suggests that primordial O2 was incorporated into the nucleus during the comet’s formation, which is unexpected given the low upper limits from remote sensing observations4. Current Solar System formation models do not predict conditions that would allow this to occur.