The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform

Kerry Peter Mahon - One of the best experts on this subject based on the ideXlab platform.

  • Combinatorial Approach to determine functional group effects on lipidoid mediated sirna delivery
    Bioconjugate Chemistry, 2010
    Co-Authors: Kerry Peter Mahon, Kevin T Love, Kathryn A Whitehead, Akin Akinc, Elizaveta S Leshchiner, Ignaty Leshchiner, Robert Langer, Dustin Anderson
    Abstract:

    The application of RNA interference (RNAi), either in the clinic or laboratory, requires safe and effective delivery methods. Here we develop a Combinatorial Approach to synthesize a library of delivery vectors based on two lipid-like substrates with known siRNA delivery capabilities. Members of this library have a mixture of lipid-like tails and feature appendages containing hydroxyl, carbamate, ether or amine functional groups as well as variations in alkyl chain length and branching. Using a luciferase reporter system in HeLa cells, we study the relationship between lipid chemical modification and delivery performance in vitro. The impact of the functional group was shown to vary depending on the overall amine content and tail number of the delivery vector. Additionally, in vivo performance was evaluated using a Factor VII knockdown assay. Two library members, each containing ether groups, were found to knock down the target protein at levels comparable to the parent delivery vector. These results demonstrate that small chemical changes to the delivery vector impact knockdown efficiency and cell viability both in vitro and in vivo. The work described here identifies new materials for siRNA delivery, as well as provides new insight into the parameters for optimized chemical makeup of lipid-like siRNA delivery materials.

  • Combinatorial Approach to determine functional group effects on lipidoid mediated sirna delivery
    Bioconjugate Chemistry, 2010
    Co-Authors: Kerry Peter Mahon, Kevin T Love, Kathryn A Whitehead, Akin Akinc, Elizaveta S Leshchiner, Ignaty Leshchiner, Robert Langer, June Qin, Daniel G Anderson
    Abstract:

    The application of RNA interference (RNAi), either in the clinic or in the laboratory, requires safe and effective delivery methods. Here, we develop a Combinatorial Approach to synthesize a library of delivery vectors based on two lipid-like substrates with known siRNA delivery capabilities. Members of this library have a mixture of lipid-like tails and feature appendages containing hydroxyl, carbamate, ether, or amine functional groups as well as variations in alkyl chain length and branching. Using a luciferase reporter system in HeLa cells, we studied the relationship between lipid chemical modification and delivery performance in vitro. The impact of the functional group was shown to vary depending on the overall amine content and tail number of the delivery vector. Additionally, in vivo performance was evaluated using a Factor VII knockdown assay. Two library members, each containing ether groups, were found to knock down the target protein at levels comparable to those of the parent delivery vector. These results demonstrate that small chemical changes to the delivery vector impact knockdown efficiency and cell viability both in vitro and in vivo. The work described here identifies new materials for siRNA delivery and provides new insight into the parameters for optimized chemical makeup of lipid-like siRNA delivery materials.

Kostya Ostrikov - One of the best experts on this subject based on the ideXlab platform.

  • effect of elemental composition and size on electron confinement in self assembled sic quantum dots a Combinatorial Approach
    Journal of Applied Physics, 2009
    Co-Authors: Das A Arulsamy, Amanda E Rider, Qijin Cheng, S Xu, Kostya Ostrikov
    Abstract:

    A high level of control over quantum dot (QD) properties such as size and composition during fabrication is required to precisely tune the eventual electronic properties of the QD. Nanoscale synthesis efforts and theoretical studies of electronic properties are traditionally treated quite separately. In this paper, a Combinatorial Approach has been taken to relate the process synthesis parameters and the electron confinement properties of the QDs. First, hybrid numerical calculations with different influx parameters for Si1−xCx QDs were carried out to simulate the changes in carbon content x and size. Second, the ionization energy theory was applied to understand the electronic properties of Si1−xCx QDs. Third, stoichiometric (x=0.5) silicon carbide QDs were grown by means of inductively coupled plasma-assisted rf magnetron sputtering. Finally, the effect of QD size and elemental composition were then incorporated in the ionization energy theory to explain the evolution of the Si1−xCx photoluminescence sp...

  • effect of elemental composition and size on electron confinement in self assembled sic quantum dots a Combinatorial Approach
    Science & Engineering Faculty, 2009
    Co-Authors: Das A Arulsamy, Amanda E Rider, Qijin Cheng, S Xu, Kostya Ostrikov
    Abstract:

    A high level of control over quantum dot (QD) properties such as size and composition during fabrication is required to precisely tune the eventual electronic properties of the QD. Nanoscale synthesis efforts and theoretical studies of electronic properties are traditionally treated quite separately. In this paper, a Combinatorial Approach has been taken to relate the process synthesis parameters and the electron confinement properties of the QDs. First, hybrid numerical calculations with different influx parameters for Si1-x Cx QDs were carried out to simulate the changes in carbon content x and size. Second, the ionization energy theory was applied to understand the electronic properties of Si1-x Cx QDs. Third, stoichiometric (x=0.5) silicon carbide QDs were grown by means of inductively coupled plasma-assisted rf magnetron sputtering. Finally, the effect of QD size and elemental composition were then incorporated in the ionization energy theory to explain the evolution of the Si1-x Cx photoluminescence spectra. These results are important for the development of deterministic synthesis Approaches of self-assembled nanoscale quantum confinement structures.

Daniel G Anderson - One of the best experts on this subject based on the ideXlab platform.

  • Combinatorial Approach to determine functional group effects on lipidoid mediated sirna delivery
    Bioconjugate Chemistry, 2010
    Co-Authors: Kerry Peter Mahon, Kevin T Love, Kathryn A Whitehead, Akin Akinc, Elizaveta S Leshchiner, Ignaty Leshchiner, Robert Langer, June Qin, Daniel G Anderson
    Abstract:

    The application of RNA interference (RNAi), either in the clinic or in the laboratory, requires safe and effective delivery methods. Here, we develop a Combinatorial Approach to synthesize a library of delivery vectors based on two lipid-like substrates with known siRNA delivery capabilities. Members of this library have a mixture of lipid-like tails and feature appendages containing hydroxyl, carbamate, ether, or amine functional groups as well as variations in alkyl chain length and branching. Using a luciferase reporter system in HeLa cells, we studied the relationship between lipid chemical modification and delivery performance in vitro. The impact of the functional group was shown to vary depending on the overall amine content and tail number of the delivery vector. Additionally, in vivo performance was evaluated using a Factor VII knockdown assay. Two library members, each containing ether groups, were found to knock down the target protein at levels comparable to those of the parent delivery vector. These results demonstrate that small chemical changes to the delivery vector impact knockdown efficiency and cell viability both in vitro and in vivo. The work described here identifies new materials for siRNA delivery and provides new insight into the parameters for optimized chemical makeup of lipid-like siRNA delivery materials.

Dustin Anderson - One of the best experts on this subject based on the ideXlab platform.

  • Combinatorial Approach to determine functional group effects on lipidoid mediated sirna delivery
    Bioconjugate Chemistry, 2010
    Co-Authors: Kerry Peter Mahon, Kevin T Love, Kathryn A Whitehead, Akin Akinc, Elizaveta S Leshchiner, Ignaty Leshchiner, Robert Langer, Dustin Anderson
    Abstract:

    The application of RNA interference (RNAi), either in the clinic or laboratory, requires safe and effective delivery methods. Here we develop a Combinatorial Approach to synthesize a library of delivery vectors based on two lipid-like substrates with known siRNA delivery capabilities. Members of this library have a mixture of lipid-like tails and feature appendages containing hydroxyl, carbamate, ether or amine functional groups as well as variations in alkyl chain length and branching. Using a luciferase reporter system in HeLa cells, we study the relationship between lipid chemical modification and delivery performance in vitro. The impact of the functional group was shown to vary depending on the overall amine content and tail number of the delivery vector. Additionally, in vivo performance was evaluated using a Factor VII knockdown assay. Two library members, each containing ether groups, were found to knock down the target protein at levels comparable to the parent delivery vector. These results demonstrate that small chemical changes to the delivery vector impact knockdown efficiency and cell viability both in vitro and in vivo. The work described here identifies new materials for siRNA delivery, as well as provides new insight into the parameters for optimized chemical makeup of lipid-like siRNA delivery materials.

Elizaveta S Leshchiner - One of the best experts on this subject based on the ideXlab platform.

  • Combinatorial Approach to determine functional group effects on lipidoid mediated sirna delivery
    Bioconjugate Chemistry, 2010
    Co-Authors: Kerry Peter Mahon, Kevin T Love, Kathryn A Whitehead, Akin Akinc, Elizaveta S Leshchiner, Ignaty Leshchiner, Robert Langer, Dustin Anderson
    Abstract:

    The application of RNA interference (RNAi), either in the clinic or laboratory, requires safe and effective delivery methods. Here we develop a Combinatorial Approach to synthesize a library of delivery vectors based on two lipid-like substrates with known siRNA delivery capabilities. Members of this library have a mixture of lipid-like tails and feature appendages containing hydroxyl, carbamate, ether or amine functional groups as well as variations in alkyl chain length and branching. Using a luciferase reporter system in HeLa cells, we study the relationship between lipid chemical modification and delivery performance in vitro. The impact of the functional group was shown to vary depending on the overall amine content and tail number of the delivery vector. Additionally, in vivo performance was evaluated using a Factor VII knockdown assay. Two library members, each containing ether groups, were found to knock down the target protein at levels comparable to the parent delivery vector. These results demonstrate that small chemical changes to the delivery vector impact knockdown efficiency and cell viability both in vitro and in vivo. The work described here identifies new materials for siRNA delivery, as well as provides new insight into the parameters for optimized chemical makeup of lipid-like siRNA delivery materials.

  • Combinatorial Approach to determine functional group effects on lipidoid mediated sirna delivery
    Bioconjugate Chemistry, 2010
    Co-Authors: Kerry Peter Mahon, Kevin T Love, Kathryn A Whitehead, Akin Akinc, Elizaveta S Leshchiner, Ignaty Leshchiner, Robert Langer, June Qin, Daniel G Anderson
    Abstract:

    The application of RNA interference (RNAi), either in the clinic or in the laboratory, requires safe and effective delivery methods. Here, we develop a Combinatorial Approach to synthesize a library of delivery vectors based on two lipid-like substrates with known siRNA delivery capabilities. Members of this library have a mixture of lipid-like tails and feature appendages containing hydroxyl, carbamate, ether, or amine functional groups as well as variations in alkyl chain length and branching. Using a luciferase reporter system in HeLa cells, we studied the relationship between lipid chemical modification and delivery performance in vitro. The impact of the functional group was shown to vary depending on the overall amine content and tail number of the delivery vector. Additionally, in vivo performance was evaluated using a Factor VII knockdown assay. Two library members, each containing ether groups, were found to knock down the target protein at levels comparable to those of the parent delivery vector. These results demonstrate that small chemical changes to the delivery vector impact knockdown efficiency and cell viability both in vitro and in vivo. The work described here identifies new materials for siRNA delivery and provides new insight into the parameters for optimized chemical makeup of lipid-like siRNA delivery materials.