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Roy A Dalmo - One of the best experts on this subject based on the ideXlab platform.
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the ontogeny of Complement Component C3 in the spotted wolffish anarhichas minor olafsen
Fish & Shellfish Immunology, 2005Co-Authors: Terje Ellingsen, Cato Strand, Eirik Monsen, Jarl Bogwald, Roy A DalmoAbstract:Abstract Eggs and larvae from the spotted wolffish ( Anarhichas minor Olafsen) were analysed to study the ontogeny of the Complement Component C3. By Western blotting experiments, the results revealed that C3 was present already in unfertilised eggs and that there was a decrease in C3 content towards hatch, whereas the results using reverse transcription–polymerase chain-reaction showed a continuous increase of C3 mRNA content towards hatch. These findings indicate a transfer of maternal Complement Component C3 to the offspring where the content of C3 decreases and the level of C3 mRNA increases towards hatch. Immunohistochemical experiments revealed that only the liver of spotted wolffish larvae contained Complement Component C3, suggesting that the liver is the main site of production.
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the spotted wolffish anarhichas minor olafsen Complement Component C3 isolation characterisation and tissue distribution
Fish & Shellfish Immunology, 2003Co-Authors: Torill K Abelseth, Terje Ellingsen, Jarl Bogwald, Sigrun Espelid, Klara Stensvag, Randi Nygaard, Roy A DalmoAbstract:Abstract The Complement Component C3 was isolated from spotted wolffish (Anarhichas minor Olafsen) serum by polyethylene glycol precipitation, anion exchange chromatography and gel filtration. Silver staining in SDS-PAGE and rabbit anti-wolffish C3 antiserum used in Western blotting revealed that spotted wolffish C3 contains two polypeptide chains, Mr65 and 115 kDa, respectively. The high molecular weight α-chain of the C3 incorporated 14C-methylamine suggesting that it contained a reactive thioester group. The deduced amino acid sequence, after screening a liver cDNA expression library, showed that the wolffish C3 contained key amino acids for binding C3 convertase, factor H, I and properdin. Also, high degree of homology to other vertebrate C3 was found in the β–α junction site. Phylogenetic tree analysis indicated that the Japanese flounder and spotted wolffish that belong to order pleuronectiformes and perciformes, respectively, are phylogenetically close species. Immunohistochemical experiments showed that liver hepatocytes and blood contained C3, and in situ hybridisation experiments revealed that liver hepatocytes expressed C3.
Terje Ellingsen - One of the best experts on this subject based on the ideXlab platform.
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the ontogeny of Complement Component C3 in the spotted wolffish anarhichas minor olafsen
Fish & Shellfish Immunology, 2005Co-Authors: Terje Ellingsen, Cato Strand, Eirik Monsen, Jarl Bogwald, Roy A DalmoAbstract:Abstract Eggs and larvae from the spotted wolffish ( Anarhichas minor Olafsen) were analysed to study the ontogeny of the Complement Component C3. By Western blotting experiments, the results revealed that C3 was present already in unfertilised eggs and that there was a decrease in C3 content towards hatch, whereas the results using reverse transcription–polymerase chain-reaction showed a continuous increase of C3 mRNA content towards hatch. These findings indicate a transfer of maternal Complement Component C3 to the offspring where the content of C3 decreases and the level of C3 mRNA increases towards hatch. Immunohistochemical experiments revealed that only the liver of spotted wolffish larvae contained Complement Component C3, suggesting that the liver is the main site of production.
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the spotted wolffish anarhichas minor olafsen Complement Component C3 isolation characterisation and tissue distribution
Fish & Shellfish Immunology, 2003Co-Authors: Torill K Abelseth, Terje Ellingsen, Jarl Bogwald, Sigrun Espelid, Klara Stensvag, Randi Nygaard, Roy A DalmoAbstract:Abstract The Complement Component C3 was isolated from spotted wolffish (Anarhichas minor Olafsen) serum by polyethylene glycol precipitation, anion exchange chromatography and gel filtration. Silver staining in SDS-PAGE and rabbit anti-wolffish C3 antiserum used in Western blotting revealed that spotted wolffish C3 contains two polypeptide chains, Mr65 and 115 kDa, respectively. The high molecular weight α-chain of the C3 incorporated 14C-methylamine suggesting that it contained a reactive thioester group. The deduced amino acid sequence, after screening a liver cDNA expression library, showed that the wolffish C3 contained key amino acids for binding C3 convertase, factor H, I and properdin. Also, high degree of homology to other vertebrate C3 was found in the β–α junction site. Phylogenetic tree analysis indicated that the Japanese flounder and spotted wolffish that belong to order pleuronectiformes and perciformes, respectively, are phylogenetically close species. Immunohistochemical experiments showed that liver hepatocytes and blood contained C3, and in situ hybridisation experiments revealed that liver hepatocytes expressed C3.
Jarl Bogwald - One of the best experts on this subject based on the ideXlab platform.
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the ontogeny of Complement Component C3 in the spotted wolffish anarhichas minor olafsen
Fish & Shellfish Immunology, 2005Co-Authors: Terje Ellingsen, Cato Strand, Eirik Monsen, Jarl Bogwald, Roy A DalmoAbstract:Abstract Eggs and larvae from the spotted wolffish ( Anarhichas minor Olafsen) were analysed to study the ontogeny of the Complement Component C3. By Western blotting experiments, the results revealed that C3 was present already in unfertilised eggs and that there was a decrease in C3 content towards hatch, whereas the results using reverse transcription–polymerase chain-reaction showed a continuous increase of C3 mRNA content towards hatch. These findings indicate a transfer of maternal Complement Component C3 to the offspring where the content of C3 decreases and the level of C3 mRNA increases towards hatch. Immunohistochemical experiments revealed that only the liver of spotted wolffish larvae contained Complement Component C3, suggesting that the liver is the main site of production.
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the spotted wolffish anarhichas minor olafsen Complement Component C3 isolation characterisation and tissue distribution
Fish & Shellfish Immunology, 2003Co-Authors: Torill K Abelseth, Terje Ellingsen, Jarl Bogwald, Sigrun Espelid, Klara Stensvag, Randi Nygaard, Roy A DalmoAbstract:Abstract The Complement Component C3 was isolated from spotted wolffish (Anarhichas minor Olafsen) serum by polyethylene glycol precipitation, anion exchange chromatography and gel filtration. Silver staining in SDS-PAGE and rabbit anti-wolffish C3 antiserum used in Western blotting revealed that spotted wolffish C3 contains two polypeptide chains, Mr65 and 115 kDa, respectively. The high molecular weight α-chain of the C3 incorporated 14C-methylamine suggesting that it contained a reactive thioester group. The deduced amino acid sequence, after screening a liver cDNA expression library, showed that the wolffish C3 contained key amino acids for binding C3 convertase, factor H, I and properdin. Also, high degree of homology to other vertebrate C3 was found in the β–α junction site. Phylogenetic tree analysis indicated that the Japanese flounder and spotted wolffish that belong to order pleuronectiformes and perciformes, respectively, are phylogenetically close species. Immunohistochemical experiments showed that liver hepatocytes and blood contained C3, and in situ hybridisation experiments revealed that liver hepatocytes expressed C3.
Sigrun Espelid - One of the best experts on this subject based on the ideXlab platform.
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Complement Component C3 transcription in atlantic halibut hippoglossus hippoglossus l larvae
Fish & Shellfish Immunology, 2006Co-Authors: Sigrun Lange, S H Bambir, Alister W Dodds, T J Bowden, I R Bricknell, Sigrun Espelid, B MagnadóttirAbstract:The Complement systems of fish are well developed and play an important role in the innate immune response. Complement C3 is the central protein of all three activation pathways and is the major opsonin of the Complement system and essential for the generation of the membrane attack complex. A 1548 bp part of Complement Component C3 was isolated from a halibut liver cDNA library by immunoscreening. The deduced amino acid sequence showed that this part of halibut C3 contained key amino acids for factor H, I and properdin binding as well as two N-glycosylation sites. Digoxigenine labelled mRNA probes were synthesised and the transcription of C3 was monitored in three larval stages at 206, 430 and 1000° d (30, 50 and 99 days post hatching), by in situ hybridisation. C3 mRNA was detected in muscle, liver, brain, chondrocytes, spinal cord, eye, intestines, oesophagus and kidney. These findings are in accordance with a former immunohistochemical study on halibut C3 protein ontogeny, indicating that C3 is indeed locally expressed in many organs from the youngest stages on. Complement may thus be linked to the formation and generation of different organs during development and play an important role in the early immune response of halibut larvae.
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the spotted wolffish anarhichas minor olafsen Complement Component C3 isolation characterisation and tissue distribution
Fish & Shellfish Immunology, 2003Co-Authors: Torill K Abelseth, Terje Ellingsen, Jarl Bogwald, Sigrun Espelid, Klara Stensvag, Randi Nygaard, Roy A DalmoAbstract:Abstract The Complement Component C3 was isolated from spotted wolffish (Anarhichas minor Olafsen) serum by polyethylene glycol precipitation, anion exchange chromatography and gel filtration. Silver staining in SDS-PAGE and rabbit anti-wolffish C3 antiserum used in Western blotting revealed that spotted wolffish C3 contains two polypeptide chains, Mr65 and 115 kDa, respectively. The high molecular weight α-chain of the C3 incorporated 14C-methylamine suggesting that it contained a reactive thioester group. The deduced amino acid sequence, after screening a liver cDNA expression library, showed that the wolffish C3 contained key amino acids for binding C3 convertase, factor H, I and properdin. Also, high degree of homology to other vertebrate C3 was found in the β–α junction site. Phylogenetic tree analysis indicated that the Japanese flounder and spotted wolffish that belong to order pleuronectiformes and perciformes, respectively, are phylogenetically close species. Immunohistochemical experiments showed that liver hepatocytes and blood contained C3, and in situ hybridisation experiments revealed that liver hepatocytes expressed C3.
Torill K Abelseth - One of the best experts on this subject based on the ideXlab platform.
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the spotted wolffish anarhichas minor olafsen Complement Component C3 isolation characterisation and tissue distribution
Fish & Shellfish Immunology, 2003Co-Authors: Torill K Abelseth, Terje Ellingsen, Jarl Bogwald, Sigrun Espelid, Klara Stensvag, Randi Nygaard, Roy A DalmoAbstract:Abstract The Complement Component C3 was isolated from spotted wolffish (Anarhichas minor Olafsen) serum by polyethylene glycol precipitation, anion exchange chromatography and gel filtration. Silver staining in SDS-PAGE and rabbit anti-wolffish C3 antiserum used in Western blotting revealed that spotted wolffish C3 contains two polypeptide chains, Mr65 and 115 kDa, respectively. The high molecular weight α-chain of the C3 incorporated 14C-methylamine suggesting that it contained a reactive thioester group. The deduced amino acid sequence, after screening a liver cDNA expression library, showed that the wolffish C3 contained key amino acids for binding C3 convertase, factor H, I and properdin. Also, high degree of homology to other vertebrate C3 was found in the β–α junction site. Phylogenetic tree analysis indicated that the Japanese flounder and spotted wolffish that belong to order pleuronectiformes and perciformes, respectively, are phylogenetically close species. Immunohistochemical experiments showed that liver hepatocytes and blood contained C3, and in situ hybridisation experiments revealed that liver hepatocytes expressed C3.