The Experts below are selected from a list of 1842 Experts worldwide ranked by ideXlab platform
John N Galgiani - One of the best experts on this subject based on the ideXlab platform.
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a quantitative enzyme linked immunoassay elisa to approximate Complement Fixing Antibody titers in serum from patients with coccidioidomycosis
Diagnostic Microbiology and Infectious Disease, 2021Co-Authors: Tao Peng, Yue Zong, Michael Dl Johnson, Sanjay V Menghani, Maria L Lewis, John N GalgianiAbstract:Abstract Coccidioidomycosis is most frequently diagnosed serologically, and the quantitative test for Complement-Fixing antibodies is considered prognostically useful. Because Complement-Fixing Antibody testing is complex, labor-intensive, and poorly standardized, an enzyme-linked immunoassay (ELISA) alternative would be attractive. In this report, we restrict the Complement-Fixing, Antibody-binding domain to a 200-amino-acid recombinant peptide of the known antigen. Over-lapping truncations of this peptide do not bind Complement-Fixing antibodies, suggesting that the responsible epitope(s) are conformational. Further, anchoring the antigenic peptide to the ELISA plate by means of a C-terminal biotin-mimic peptide tag instead of allowing the peptide to randomly adhere to the plastic plate improves sensitivity of Antibody detection by 1–2 logs in different sera. The newly developed ELISA shows a significant quantitative correlation with Complement-Fixing Antibody titers. This ELISA shows potential as the basis for a new quantitative assay for coccidioidal antibodies.
Tao Peng - One of the best experts on this subject based on the ideXlab platform.
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a quantitative enzyme linked immunoassay elisa to approximate Complement Fixing Antibody titers in serum from patients with coccidioidomycosis
Diagnostic Microbiology and Infectious Disease, 2021Co-Authors: Tao Peng, Yue Zong, Michael Dl Johnson, Sanjay V Menghani, Maria L Lewis, John N GalgianiAbstract:Abstract Coccidioidomycosis is most frequently diagnosed serologically, and the quantitative test for Complement-Fixing antibodies is considered prognostically useful. Because Complement-Fixing Antibody testing is complex, labor-intensive, and poorly standardized, an enzyme-linked immunoassay (ELISA) alternative would be attractive. In this report, we restrict the Complement-Fixing, Antibody-binding domain to a 200-amino-acid recombinant peptide of the known antigen. Over-lapping truncations of this peptide do not bind Complement-Fixing antibodies, suggesting that the responsible epitope(s) are conformational. Further, anchoring the antigenic peptide to the ELISA plate by means of a C-terminal biotin-mimic peptide tag instead of allowing the peptide to randomly adhere to the plastic plate improves sensitivity of Antibody detection by 1–2 logs in different sera. The newly developed ELISA shows a significant quantitative correlation with Complement-Fixing Antibody titers. This ELISA shows potential as the basis for a new quantitative assay for coccidioidal antibodies.
Maria L Lewis - One of the best experts on this subject based on the ideXlab platform.
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a quantitative enzyme linked immunoassay elisa to approximate Complement Fixing Antibody titers in serum from patients with coccidioidomycosis
Diagnostic Microbiology and Infectious Disease, 2021Co-Authors: Tao Peng, Yue Zong, Michael Dl Johnson, Sanjay V Menghani, Maria L Lewis, John N GalgianiAbstract:Abstract Coccidioidomycosis is most frequently diagnosed serologically, and the quantitative test for Complement-Fixing antibodies is considered prognostically useful. Because Complement-Fixing Antibody testing is complex, labor-intensive, and poorly standardized, an enzyme-linked immunoassay (ELISA) alternative would be attractive. In this report, we restrict the Complement-Fixing, Antibody-binding domain to a 200-amino-acid recombinant peptide of the known antigen. Over-lapping truncations of this peptide do not bind Complement-Fixing antibodies, suggesting that the responsible epitope(s) are conformational. Further, anchoring the antigenic peptide to the ELISA plate by means of a C-terminal biotin-mimic peptide tag instead of allowing the peptide to randomly adhere to the plastic plate improves sensitivity of Antibody detection by 1–2 logs in different sera. The newly developed ELISA shows a significant quantitative correlation with Complement-Fixing Antibody titers. This ELISA shows potential as the basis for a new quantitative assay for coccidioidal antibodies.
Sanjay V Menghani - One of the best experts on this subject based on the ideXlab platform.
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a quantitative enzyme linked immunoassay elisa to approximate Complement Fixing Antibody titers in serum from patients with coccidioidomycosis
Diagnostic Microbiology and Infectious Disease, 2021Co-Authors: Tao Peng, Yue Zong, Michael Dl Johnson, Sanjay V Menghani, Maria L Lewis, John N GalgianiAbstract:Abstract Coccidioidomycosis is most frequently diagnosed serologically, and the quantitative test for Complement-Fixing antibodies is considered prognostically useful. Because Complement-Fixing Antibody testing is complex, labor-intensive, and poorly standardized, an enzyme-linked immunoassay (ELISA) alternative would be attractive. In this report, we restrict the Complement-Fixing, Antibody-binding domain to a 200-amino-acid recombinant peptide of the known antigen. Over-lapping truncations of this peptide do not bind Complement-Fixing antibodies, suggesting that the responsible epitope(s) are conformational. Further, anchoring the antigenic peptide to the ELISA plate by means of a C-terminal biotin-mimic peptide tag instead of allowing the peptide to randomly adhere to the plastic plate improves sensitivity of Antibody detection by 1–2 logs in different sera. The newly developed ELISA shows a significant quantitative correlation with Complement-Fixing Antibody titers. This ELISA shows potential as the basis for a new quantitative assay for coccidioidal antibodies.
Michael Dl Johnson - One of the best experts on this subject based on the ideXlab platform.
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a quantitative enzyme linked immunoassay elisa to approximate Complement Fixing Antibody titers in serum from patients with coccidioidomycosis
Diagnostic Microbiology and Infectious Disease, 2021Co-Authors: Tao Peng, Yue Zong, Michael Dl Johnson, Sanjay V Menghani, Maria L Lewis, John N GalgianiAbstract:Abstract Coccidioidomycosis is most frequently diagnosed serologically, and the quantitative test for Complement-Fixing antibodies is considered prognostically useful. Because Complement-Fixing Antibody testing is complex, labor-intensive, and poorly standardized, an enzyme-linked immunoassay (ELISA) alternative would be attractive. In this report, we restrict the Complement-Fixing, Antibody-binding domain to a 200-amino-acid recombinant peptide of the known antigen. Over-lapping truncations of this peptide do not bind Complement-Fixing antibodies, suggesting that the responsible epitope(s) are conformational. Further, anchoring the antigenic peptide to the ELISA plate by means of a C-terminal biotin-mimic peptide tag instead of allowing the peptide to randomly adhere to the plastic plate improves sensitivity of Antibody detection by 1–2 logs in different sera. The newly developed ELISA shows a significant quantitative correlation with Complement-Fixing Antibody titers. This ELISA shows potential as the basis for a new quantitative assay for coccidioidal antibodies.