The Experts below are selected from a list of 159 Experts worldwide ranked by ideXlab platform
Jesper Wengel - One of the best experts on this subject based on the ideXlab platform.
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Locked nucleic acids: promising nucleic acid analogs for therapeutic applications
Chemistry & Biodiversity, 2010Co-Authors: Rakesh Naduvile Veedu, Jesper WengelAbstract:Locked Nucleic Acid (LNA) is a unique nucleic-acid modification possessing very high binding affinity and excellent specificity toward Complementary RNA or DNA oligonucleotides. The remarkable properties exhibited by LNA oligonucleotides have been employed in different nucleic acid-based therapeutic strategies both in vitro and in vivo. Herein, we highlight the applications of LNA nucleotides for controlling gene expression.
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locked nucleic acid high affinity targeting of Complementary RNA for rnomics
Handbook of experimental pharmacology, 2006Co-Authors: Sakari Kauppinen, Birte Vester, Jesper WengelAbstract:Locked nucleic acid (LNA) is a nucleic acid analog containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA-mimicking sugar conformation. This conformational restriction is translated into unprecedented hybridization affinity towards Complementary single-stranded RNA molecules. That makes fully modified LNAs, LNA/DNA mixmers, or LNA/RNA mixmers uniquely suited for mimicking RNA structures and for RNA targeting in vitro or in vivo. The focus of this chapter is on LNA antisense, LNA-modified DNAzymes (LNAzymes), LNA-modified small interfering (si)RNA (siLNA), LNA-enhanced expression profiling by real-time RT-PCR and detection and analysis of microRNAs by LNA-modified probes.
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lna locked nucleic acid high affinity targeting of Complementary RNA and dna
Biochemistry, 2004Co-Authors: Birte Vester, Jesper WengelAbstract:Locked nucleic acid (LNA) is a nucleic acid analogue containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA mimicking sugar conformation. LNA oligonucleotides display unprecedented hybridization affinity toward Complementary single-stranded RNA and Complementary single- or double-stranded DNA. Structural studies have shown that LNA oligonucleotides induce A-type (RNA-like) duplex conformations. The wide applicability of LNA oligonucleotides for gene silencing and their use for research and diagnostic purposes are documented in a number of recent reports, some of which are described herein.
Birte Vester - One of the best experts on this subject based on the ideXlab platform.
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locked nucleic acid high affinity targeting of Complementary RNA for rnomics
Handbook of experimental pharmacology, 2006Co-Authors: Sakari Kauppinen, Birte Vester, Jesper WengelAbstract:Locked nucleic acid (LNA) is a nucleic acid analog containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA-mimicking sugar conformation. This conformational restriction is translated into unprecedented hybridization affinity towards Complementary single-stranded RNA molecules. That makes fully modified LNAs, LNA/DNA mixmers, or LNA/RNA mixmers uniquely suited for mimicking RNA structures and for RNA targeting in vitro or in vivo. The focus of this chapter is on LNA antisense, LNA-modified DNAzymes (LNAzymes), LNA-modified small interfering (si)RNA (siLNA), LNA-enhanced expression profiling by real-time RT-PCR and detection and analysis of microRNAs by LNA-modified probes.
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lna locked nucleic acid high affinity targeting of Complementary RNA and dna
Biochemistry, 2004Co-Authors: Birte Vester, Jesper WengelAbstract:Locked nucleic acid (LNA) is a nucleic acid analogue containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA mimicking sugar conformation. LNA oligonucleotides display unprecedented hybridization affinity toward Complementary single-stranded RNA and Complementary single- or double-stranded DNA. Structural studies have shown that LNA oligonucleotides induce A-type (RNA-like) duplex conformations. The wide applicability of LNA oligonucleotides for gene silencing and their use for research and diagnostic purposes are documented in a number of recent reports, some of which are described herein.
Takeshi Wada - One of the best experts on this subject based on the ideXlab platform.
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Enhancement of the affinity of 2′-O-Me-oligonucleotides for Complementary RNA by incorporating a stereoregulated boranophosphate backbone
RSC Advances, 2020Co-Authors: Yohei Nukaga, Tetsuhiko Takemura, Naoki Iwamoto, Takeshi WadaAbstract:2′-O-Me-oligoribonucleotides bearing a stereoregulated boranophosphate backbone (2′-O-Me-PB-ORNs) were synthesized by using 2′-O-Me-ribonucleoside 3′-O-oxazaphospholidine monomers. A thermal denaturation study of the resultant diastereopure 2′-O-Me-PB-ORNs revealed that an all-(Sp)-boranophosphate backbone had a large stabilizing effect on the duplex with Complementary RNA.
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enhancement of the affinity of 2 o me oligonucleotides for Complementary RNA by incorporating a stereoregulated boranophosphate backbone
RSC Advances, 2015Co-Authors: Yohei Nukaga, Tetsuhiko Takemura, Naoki Iwamoto, Takeshi WadaAbstract:2′-O-Me-oligoribonucleotides bearing a stereoregulated boranophosphate backbone (2′-O-Me-PB-ORNs) were synthesized by using 2′-O-Me-ribonucleoside 3′-O-oxazaphospholidine monomers. A thermal denaturation study of the resultant diastereopure 2′-O-Me-PB-ORNs revealed that an all-(Sp)-boranophosphate backbone had a large stabilizing effect on the duplex with Complementary RNA.
Yohei Nukaga - One of the best experts on this subject based on the ideXlab platform.
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Enhancement of the affinity of 2′-O-Me-oligonucleotides for Complementary RNA by incorporating a stereoregulated boranophosphate backbone
RSC Advances, 2020Co-Authors: Yohei Nukaga, Tetsuhiko Takemura, Naoki Iwamoto, Takeshi WadaAbstract:2′-O-Me-oligoribonucleotides bearing a stereoregulated boranophosphate backbone (2′-O-Me-PB-ORNs) were synthesized by using 2′-O-Me-ribonucleoside 3′-O-oxazaphospholidine monomers. A thermal denaturation study of the resultant diastereopure 2′-O-Me-PB-ORNs revealed that an all-(Sp)-boranophosphate backbone had a large stabilizing effect on the duplex with Complementary RNA.
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enhancement of the affinity of 2 o me oligonucleotides for Complementary RNA by incorporating a stereoregulated boranophosphate backbone
RSC Advances, 2015Co-Authors: Yohei Nukaga, Tetsuhiko Takemura, Naoki Iwamoto, Takeshi WadaAbstract:2′-O-Me-oligoribonucleotides bearing a stereoregulated boranophosphate backbone (2′-O-Me-PB-ORNs) were synthesized by using 2′-O-Me-ribonucleoside 3′-O-oxazaphospholidine monomers. A thermal denaturation study of the resultant diastereopure 2′-O-Me-PB-ORNs revealed that an all-(Sp)-boranophosphate backbone had a large stabilizing effect on the duplex with Complementary RNA.
Sakari Kauppinen - One of the best experts on this subject based on the ideXlab platform.
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locked nucleic acid high affinity targeting of Complementary RNA for rnomics
Handbook of experimental pharmacology, 2006Co-Authors: Sakari Kauppinen, Birte Vester, Jesper WengelAbstract:Locked nucleic acid (LNA) is a nucleic acid analog containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA-mimicking sugar conformation. This conformational restriction is translated into unprecedented hybridization affinity towards Complementary single-stranded RNA molecules. That makes fully modified LNAs, LNA/DNA mixmers, or LNA/RNA mixmers uniquely suited for mimicking RNA structures and for RNA targeting in vitro or in vivo. The focus of this chapter is on LNA antisense, LNA-modified DNAzymes (LNAzymes), LNA-modified small interfering (si)RNA (siLNA), LNA-enhanced expression profiling by real-time RT-PCR and detection and analysis of microRNAs by LNA-modified probes.