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Marc Baldus - One of the best experts on this subject based on the ideXlab platform.

  • Solid-state NMR on Complex Biomolecules: Methods and applications
    Modern Magnetic Resonance, 2018
    Co-Authors: Deni Mance, Markus Weingarth, Marc Baldus
    Abstract:

    Solid-state NMR (ssNMR) can provide structural information at the most detailed level and, at the same time, is applicable in highly heterogeneous and Complex molecular environments, largely irrespective of solubility or crystallinity. In the following chapter, we discuss concepts to deal with the spectroscopic challenges of applying ssNMR to Complex biomolecular systems and how to place structural information obtained from ssNMR in a (supra)molecular context. Applications range from protein biopolymers and hydrogels to drug delivery systems, biosilica, and other biomaterials.

  • Fractional deuteration applied to biomolecular solid-state NMR spectroscopy
    Journal of Biomolecular NMR, 2012
    Co-Authors: Deepak Nand, Abhishek Cukkemane, Stefan Becker, Marc Baldus
    Abstract:

    Solid-state Nuclear Magnetic Resonance can provide detailed insight into structural and dynamical aspects of Complex Biomolecules. With increasing molecular size, advanced approaches for spectral simplification and the detection of medium to long-range contacts become of critical relevance. We have analyzed the protonation pattern of a membrane-embedded ion channel that was obtained from bacterial expression using protonated precursors and D(2)O medium. We find an overall reduction of 50% in protein protonation. High levels of deuteration at H(α) and H(β) positions reduce spectral congestion in ((1)H,(13)C,(15)N) correlation experiments and generate a transfer profile in longitudinal mixing schemes that can be tuned to specific resonance frequencies. At the same time, residual protons are predominantly found at amino-acid side-chain positions enhancing the prospects for obtaining side-chain resonance assignments and for detecting medium to long-range contacts. Fractional deuteration thus provides a powerful means to aid the structural analysis of Complex Biomolecules by solid-state NMR.

  • eMagRes - Baldus, Marc: Solid‐state NMR: Quantum Mechanics at Work in a Biophysical Context
    Encyclopedia of Magnetic Resonance, 2010
    Co-Authors: Marc Baldus
    Abstract:

    Solid-state NMR provides a rich source for the study of biophysical problems at a quantum-mechanical level. In my career as an NMR researcher, I was fortunate to learn and further develop, in leading NMR laboratories, the methodological basis to use high-resolution solid-state NMR in a biophysical context. In this article, the development of these techniques and some of their applications to Complex Biomolecules are discussed. Keywords: Floquet theory; MAS; membrane proteins; recoupling; solid-state NMR

  • Solid‐State NMR Spectroscopy on Complex Biomolecules
    Angewandte Chemie (International ed. in English), 2010
    Co-Authors: M Renault, Abhishek Cukkemane, Marc Baldus
    Abstract:

    Biomolecular applications of NMR spectroscopy are often merely associated with soluble molecules or magnetic resonance imaging. However, since the late 1970s, solid-state NMR (ssNMR) spectroscopy has demonstrated its ability to provide atomic-level insight into Complex biomolecular systems ranging from lipid bilayers to Complex biomaterials. In the last decade, progress in the areas of NMR spectroscopy, biophysics, and molecular biology have significantly expanded the repertoire of ssNMR spectroscopy for biomolecular studies. This Review discusses current approaches and methodological challenges, and highlights recent progress in using ssNMR spectroscopy at the interface of structural and cellular biology.

  • Solid-state NMR spectroscopy on Complex Biomolecules
    Angewandte Chemie - International Edition, 2010
    Co-Authors: M Renault, Abhishek Cukkemane, Marc Baldus
    Abstract:

    Biomolecular applications of NMR spectroscopy are often merely associated with soluble molecules or magnetic resonance imaging. However, since the late 1970s, solid-state NMR (ssNMR) spectroscopy has demonstrated its ability to provide atomic-level insight into Complex biomolecular systems ranging from lipid bilayers to Complex biomaterials. In the last decade, progress in the areas of NMR spectroscopy, biophysics, and molecular biology have significantly expanded the repertoire of ssNMR spectroscopy for biomolecular studies. This Review discusses current approaches and methodological challenges, and highlights recent progress in using ssNMR spectroscopy at the interface of structural and cellular biology.

Abhishek Cukkemane - One of the best experts on this subject based on the ideXlab platform.

  • Fractional deuteration applied to biomolecular solid-state NMR spectroscopy
    Journal of Biomolecular NMR, 2012
    Co-Authors: Deepak Nand, Abhishek Cukkemane, Stefan Becker, Marc Baldus
    Abstract:

    Solid-state Nuclear Magnetic Resonance can provide detailed insight into structural and dynamical aspects of Complex Biomolecules. With increasing molecular size, advanced approaches for spectral simplification and the detection of medium to long-range contacts become of critical relevance. We have analyzed the protonation pattern of a membrane-embedded ion channel that was obtained from bacterial expression using protonated precursors and D(2)O medium. We find an overall reduction of 50% in protein protonation. High levels of deuteration at H(α) and H(β) positions reduce spectral congestion in ((1)H,(13)C,(15)N) correlation experiments and generate a transfer profile in longitudinal mixing schemes that can be tuned to specific resonance frequencies. At the same time, residual protons are predominantly found at amino-acid side-chain positions enhancing the prospects for obtaining side-chain resonance assignments and for detecting medium to long-range contacts. Fractional deuteration thus provides a powerful means to aid the structural analysis of Complex Biomolecules by solid-state NMR.

  • Solid‐State NMR Spectroscopy on Complex Biomolecules
    Angewandte Chemie (International ed. in English), 2010
    Co-Authors: M Renault, Abhishek Cukkemane, Marc Baldus
    Abstract:

    Biomolecular applications of NMR spectroscopy are often merely associated with soluble molecules or magnetic resonance imaging. However, since the late 1970s, solid-state NMR (ssNMR) spectroscopy has demonstrated its ability to provide atomic-level insight into Complex biomolecular systems ranging from lipid bilayers to Complex biomaterials. In the last decade, progress in the areas of NMR spectroscopy, biophysics, and molecular biology have significantly expanded the repertoire of ssNMR spectroscopy for biomolecular studies. This Review discusses current approaches and methodological challenges, and highlights recent progress in using ssNMR spectroscopy at the interface of structural and cellular biology.

  • Solid-state NMR spectroscopy on Complex Biomolecules
    Angewandte Chemie - International Edition, 2010
    Co-Authors: M Renault, Abhishek Cukkemane, Marc Baldus
    Abstract:

    Biomolecular applications of NMR spectroscopy are often merely associated with soluble molecules or magnetic resonance imaging. However, since the late 1970s, solid-state NMR (ssNMR) spectroscopy has demonstrated its ability to provide atomic-level insight into Complex biomolecular systems ranging from lipid bilayers to Complex biomaterials. In the last decade, progress in the areas of NMR spectroscopy, biophysics, and molecular biology have significantly expanded the repertoire of ssNMR spectroscopy for biomolecular studies. This Review discusses current approaches and methodological challenges, and highlights recent progress in using ssNMR spectroscopy at the interface of structural and cellular biology.

M Renault - One of the best experts on this subject based on the ideXlab platform.

  • Solid‐State NMR Spectroscopy on Complex Biomolecules
    Angewandte Chemie (International ed. in English), 2010
    Co-Authors: M Renault, Abhishek Cukkemane, Marc Baldus
    Abstract:

    Biomolecular applications of NMR spectroscopy are often merely associated with soluble molecules or magnetic resonance imaging. However, since the late 1970s, solid-state NMR (ssNMR) spectroscopy has demonstrated its ability to provide atomic-level insight into Complex biomolecular systems ranging from lipid bilayers to Complex biomaterials. In the last decade, progress in the areas of NMR spectroscopy, biophysics, and molecular biology have significantly expanded the repertoire of ssNMR spectroscopy for biomolecular studies. This Review discusses current approaches and methodological challenges, and highlights recent progress in using ssNMR spectroscopy at the interface of structural and cellular biology.

  • Solid-state NMR spectroscopy on Complex Biomolecules
    Angewandte Chemie - International Edition, 2010
    Co-Authors: M Renault, Abhishek Cukkemane, Marc Baldus
    Abstract:

    Biomolecular applications of NMR spectroscopy are often merely associated with soluble molecules or magnetic resonance imaging. However, since the late 1970s, solid-state NMR (ssNMR) spectroscopy has demonstrated its ability to provide atomic-level insight into Complex biomolecular systems ranging from lipid bilayers to Complex biomaterials. In the last decade, progress in the areas of NMR spectroscopy, biophysics, and molecular biology have significantly expanded the repertoire of ssNMR spectroscopy for biomolecular studies. This Review discusses current approaches and methodological challenges, and highlights recent progress in using ssNMR spectroscopy at the interface of structural and cellular biology.

Christoph Bräuchle - One of the best experts on this subject based on the ideXlab platform.

  • Metal-enhanced fluorescence of chlorophylls in single light-harvesting Complexes.
    Nano letters, 2007
    Co-Authors: Sebastian Mackowski, Stephan Wörmke, Achim Hartschuh, Tatas H. P. Brotosudarmo, Hugo Scheer, Alexander O. Govorov, Andreas J. Maier, Hayk Harutyunyan, Christoph Bräuchle
    Abstract:

    Ensemble and single-molecule spectroscopy demonstrates that both emission and absorption of peridinin−chlorophyll−protein photosynthetic antennae can be largely enhanced through plasmonic interactions. We find up to 18-fold increase of the chlorophyll fluorescence for Complexes placed near a silver metal layer. This enhancement, which leaves no measurable effects on the protein structure, is observed when exciting either chlorophyll or carotenoid and is attributed predominantly to an increase of the excitation rate in the antenna. The enhancement mechanism comes from plasmon-induced amplification of electromagnetic fields inside the Complex. This result is an important step toward applying plasmonic nanostructures for controlling the optical response of Complex Biomolecules and improving the design and functioning of artificial light-harvesting systems.

Sebastian Mackowski - One of the best experts on this subject based on the ideXlab platform.

  • Metal - Enhanced fluorescence of chlorophylls in single light - Harvesting Complexes
    MRS Proceedings, 2009
    Co-Authors: Sebastian Mackowski, D. Piatkowski, Stephan Wörmke, Achim Hartschuh, Christoph Bräeuchle, Tatas H. P. Brotosudarmo, Hugo Scheer, Ashish Agarwal, Nicholas A. Kotov, Alexander O. Govorov
    Abstract:

    AbstractWe show that the fluorescence of peridinin-chlorophyll a-protein Complexes can be strongly enhanced via coupling with plasmon excitations localized in metal nanostructures. The results of ensemble and single-molecule spectroscopy experiments at room temperature demonstrate six-fold increase of the emission intensity of the light-harvesting Complex when it is placed in the vicinity of chemically prepared silver islands. Irrespective of the enhancement, we observe no effect of the metal nanoparticle on the fluorescence emission energy of the Complex. This observation implies that plasmon excitations may be applied for controlling the optical properties of Complex Biomolecules.

  • Metal-enhanced fluorescence of chlorophylls in single light-harvesting Complexes.
    Nano letters, 2007
    Co-Authors: Sebastian Mackowski, Stephan Wörmke, Achim Hartschuh, Tatas H. P. Brotosudarmo, Hugo Scheer, Alexander O. Govorov, Andreas J. Maier, Hayk Harutyunyan, Christoph Bräuchle
    Abstract:

    Ensemble and single-molecule spectroscopy demonstrates that both emission and absorption of peridinin−chlorophyll−protein photosynthetic antennae can be largely enhanced through plasmonic interactions. We find up to 18-fold increase of the chlorophyll fluorescence for Complexes placed near a silver metal layer. This enhancement, which leaves no measurable effects on the protein structure, is observed when exciting either chlorophyll or carotenoid and is attributed predominantly to an increase of the excitation rate in the antenna. The enhancement mechanism comes from plasmon-induced amplification of electromagnetic fields inside the Complex. This result is an important step toward applying plasmonic nanostructures for controlling the optical response of Complex Biomolecules and improving the design and functioning of artificial light-harvesting systems.