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Grant M Hatch - One of the best experts on this subject based on the ideXlab platform.
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Complex Expression pattern of the barth syndrome gene product tafazzin in human cell lines and murine tissues
Biochemistry and Cell Biology, 2004Co-Authors: Biao Lu, Marguerite R Kelher, Tal M Lewin, Rosalind A. Coleman, Patrick C. Choy, Grant M HatchAbstract:Tafazzins, a group of proteins that are defective in patients with Barth syndrome, are produced by alternate splicing of the gene G4.5 or TAZ. RT-PCR and transcription-coupled in vitro translation analysis were undertaken to determine the Expression of alternatively spliced TAZ mRNA in mouse tissues and human cell lines. Only two tafazzin transcripts, both lacking exon 5, were expressed in murine tissues, whereas four tafazzin transcripts, all lacking exon 5, were observed in human umbilical vein vascular endothelial cells and U937 human monoblasts indicating a species-specific difference in the Expression of TAZ mRNAs in mouse and humans. Only TAZ lacking exon 5 was expressed in murine heart. Differentiation of U937 human monoblasts into macrophages did not alter Expression of the tafazzin transcripts indicating that TAZ Expression is independent of monocyte differentiation. Cloning and in vitro Expression of both murine and human tafazzin cDNA revealed two prominent protein bands that corresponded to th...
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Complex Expression pattern of the Barth syndrome gene product tafazzin in human cell lines and murine tissues.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 2004Co-Authors: Marguerite R Kelher, Tal M Lewin, Rosalind A. Coleman, Patrick C. Choy, Douglas P Lee, Grant M HatchAbstract:Tafazzins, a group of proteins that are defective in patients with Barth syndrome, are produced by alternate splicing of the gene G4.5 or TAZ. RT-PCR and transcription-coupled in vitro translation ...
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Complex Expression pattern of the barth syndrome gene product tafazzin in human cell lines and murine tissues
Biochemistry and Cell Biology, 2004Co-Authors: Marguerite R Kelher, Tal M Lewin, Rosalind A. Coleman, Patrick C. Choy, Douglas P Lee, Grant M HatchAbstract:Tafazzins, a group of proteins that are defective in patients with Barth syndrome, are produced by alternate splicing of the gene G4.5 or TAZ. RT-PCR and transcription-coupled in vitro translation analysis were undertaken to determine the Expression of alternatively spliced TAZ mRNA in mouse tissues and human cell lines. Only two tafazzin transcripts, both lacking exon 5, were expressed in murine tissues, whereas four tafazzin transcripts, all lacking exon 5, were observed in human umbilical vein vascular endothelial cells and U937 human monoblasts indicating a species-specific difference in the Expression of TAZ mRNAs in mouse and humans. Only TAZ lacking exon 5 was expressed in murine heart. Differentiation of U937 human monoblasts into macrophages did not alter Expression of the tafazzin transcripts indicating that TAZ Expression is independent of monocyte differentiation. Cloning and in vitro Expression of both murine and human tafazzin cDNA revealed two prominent protein bands that corresponded to the expected sizes of alternative translation. A novel fifth motif, identified as critical for the glycerolphosphate acyltransferase family, was observed in human tafazzin. The presence of a mutation in this region in Barth syndrome patients indicates that this motif is essential for tafazzin function.
Marguerite R Kelher - One of the best experts on this subject based on the ideXlab platform.
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Complex Expression pattern of the barth syndrome gene product tafazzin in human cell lines and murine tissues
Biochemistry and Cell Biology, 2004Co-Authors: Biao Lu, Marguerite R Kelher, Tal M Lewin, Rosalind A. Coleman, Patrick C. Choy, Grant M HatchAbstract:Tafazzins, a group of proteins that are defective in patients with Barth syndrome, are produced by alternate splicing of the gene G4.5 or TAZ. RT-PCR and transcription-coupled in vitro translation analysis were undertaken to determine the Expression of alternatively spliced TAZ mRNA in mouse tissues and human cell lines. Only two tafazzin transcripts, both lacking exon 5, were expressed in murine tissues, whereas four tafazzin transcripts, all lacking exon 5, were observed in human umbilical vein vascular endothelial cells and U937 human monoblasts indicating a species-specific difference in the Expression of TAZ mRNAs in mouse and humans. Only TAZ lacking exon 5 was expressed in murine heart. Differentiation of U937 human monoblasts into macrophages did not alter Expression of the tafazzin transcripts indicating that TAZ Expression is independent of monocyte differentiation. Cloning and in vitro Expression of both murine and human tafazzin cDNA revealed two prominent protein bands that corresponded to th...
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Complex Expression pattern of the Barth syndrome gene product tafazzin in human cell lines and murine tissues.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 2004Co-Authors: Marguerite R Kelher, Tal M Lewin, Rosalind A. Coleman, Patrick C. Choy, Douglas P Lee, Grant M HatchAbstract:Tafazzins, a group of proteins that are defective in patients with Barth syndrome, are produced by alternate splicing of the gene G4.5 or TAZ. RT-PCR and transcription-coupled in vitro translation ...
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Complex Expression pattern of the barth syndrome gene product tafazzin in human cell lines and murine tissues
Biochemistry and Cell Biology, 2004Co-Authors: Marguerite R Kelher, Tal M Lewin, Rosalind A. Coleman, Patrick C. Choy, Douglas P Lee, Grant M HatchAbstract:Tafazzins, a group of proteins that are defective in patients with Barth syndrome, are produced by alternate splicing of the gene G4.5 or TAZ. RT-PCR and transcription-coupled in vitro translation analysis were undertaken to determine the Expression of alternatively spliced TAZ mRNA in mouse tissues and human cell lines. Only two tafazzin transcripts, both lacking exon 5, were expressed in murine tissues, whereas four tafazzin transcripts, all lacking exon 5, were observed in human umbilical vein vascular endothelial cells and U937 human monoblasts indicating a species-specific difference in the Expression of TAZ mRNAs in mouse and humans. Only TAZ lacking exon 5 was expressed in murine heart. Differentiation of U937 human monoblasts into macrophages did not alter Expression of the tafazzin transcripts indicating that TAZ Expression is independent of monocyte differentiation. Cloning and in vitro Expression of both murine and human tafazzin cDNA revealed two prominent protein bands that corresponded to the expected sizes of alternative translation. A novel fifth motif, identified as critical for the glycerolphosphate acyltransferase family, was observed in human tafazzin. The presence of a mutation in this region in Barth syndrome patients indicates that this motif is essential for tafazzin function.
Benjamin F Voight - One of the best experts on this subject based on the ideXlab platform.
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The Nephila clavipes genome highlights the diversity of spider silk genes and their Complex Expression
Nature Genetics, 2017Co-Authors: Paul L Babb, Cheryl Y. Hayashi, Nicholas F Lahens, David N Nicholson, John B Hogenesch, Linden Higgins, Matjaž Kuntner, Ingi Agnarsson, Sandra M. Correa-garhwal, Benjamin F VoightAbstract:Spider silks are the toughest known biological materials, yet are lightweight and virtually invisible to the human immune system, and they thus have revolutionary potential for medicine and industry. Spider silks are largely composed of spidroins, a unique family of structural proteins. To investigate spidroin genes systematically, we constructed the first genome of an orb-weaving spider: the golden orb-weaver ( Nephila clavipes ), which builds large webs using an extensive repertoire of silks with diverse physical properties. We cataloged 28 Nephila spidroins, representing all known orb-weaver spidroin types, and identified 394 repeated coding motif variants and higher-order repetitive cassette structures unique to specific spidroins. Characterization of spidroin Expression in distinct silk gland types indicates that glands can express multiple spidroin types. We find evidence of an alternatively spliced spidroin, a spidroin expressed only in venom glands, evolutionary mechanisms for spidroin diversification, and non-spidroin genes with Expression patterns that suggest roles in silk production. Benjamin Voight and colleagues report the annotated genome of the golden orb-weaver spider. They describe 28 spider silk genes (spidroins), characterize their Expression in distinct silk gland types and identify non-spidroin genes with Expression patterns suggesting potential roles in silk production.
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the nephila clavipes genome highlights the diversity of spider silk genes and their Complex Expression
Nature Genetics, 2017Co-Authors: Paul L Babb, Cheryl Y. Hayashi, Nicholas F Lahens, David N Nicholson, John B Hogenesch, Linden Higgins, Sandra M Correagarhwal, Matjaž Kuntner, Ingi Agnarsson, Benjamin F VoightAbstract:Benjamin Voight and colleagues report the annotated genome of the golden orb-weaver spider. They describe 28 spider silk genes (spidroins), characterize their Expression in distinct silk gland types and identify non-spidroin genes with Expression patterns suggesting potential roles in silk production.
Tal M Lewin - One of the best experts on this subject based on the ideXlab platform.
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Complex Expression pattern of the barth syndrome gene product tafazzin in human cell lines and murine tissues
Biochemistry and Cell Biology, 2004Co-Authors: Biao Lu, Marguerite R Kelher, Tal M Lewin, Rosalind A. Coleman, Patrick C. Choy, Grant M HatchAbstract:Tafazzins, a group of proteins that are defective in patients with Barth syndrome, are produced by alternate splicing of the gene G4.5 or TAZ. RT-PCR and transcription-coupled in vitro translation analysis were undertaken to determine the Expression of alternatively spliced TAZ mRNA in mouse tissues and human cell lines. Only two tafazzin transcripts, both lacking exon 5, were expressed in murine tissues, whereas four tafazzin transcripts, all lacking exon 5, were observed in human umbilical vein vascular endothelial cells and U937 human monoblasts indicating a species-specific difference in the Expression of TAZ mRNAs in mouse and humans. Only TAZ lacking exon 5 was expressed in murine heart. Differentiation of U937 human monoblasts into macrophages did not alter Expression of the tafazzin transcripts indicating that TAZ Expression is independent of monocyte differentiation. Cloning and in vitro Expression of both murine and human tafazzin cDNA revealed two prominent protein bands that corresponded to th...
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Complex Expression pattern of the Barth syndrome gene product tafazzin in human cell lines and murine tissues.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 2004Co-Authors: Marguerite R Kelher, Tal M Lewin, Rosalind A. Coleman, Patrick C. Choy, Douglas P Lee, Grant M HatchAbstract:Tafazzins, a group of proteins that are defective in patients with Barth syndrome, are produced by alternate splicing of the gene G4.5 or TAZ. RT-PCR and transcription-coupled in vitro translation ...
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Complex Expression pattern of the barth syndrome gene product tafazzin in human cell lines and murine tissues
Biochemistry and Cell Biology, 2004Co-Authors: Marguerite R Kelher, Tal M Lewin, Rosalind A. Coleman, Patrick C. Choy, Douglas P Lee, Grant M HatchAbstract:Tafazzins, a group of proteins that are defective in patients with Barth syndrome, are produced by alternate splicing of the gene G4.5 or TAZ. RT-PCR and transcription-coupled in vitro translation analysis were undertaken to determine the Expression of alternatively spliced TAZ mRNA in mouse tissues and human cell lines. Only two tafazzin transcripts, both lacking exon 5, were expressed in murine tissues, whereas four tafazzin transcripts, all lacking exon 5, were observed in human umbilical vein vascular endothelial cells and U937 human monoblasts indicating a species-specific difference in the Expression of TAZ mRNAs in mouse and humans. Only TAZ lacking exon 5 was expressed in murine heart. Differentiation of U937 human monoblasts into macrophages did not alter Expression of the tafazzin transcripts indicating that TAZ Expression is independent of monocyte differentiation. Cloning and in vitro Expression of both murine and human tafazzin cDNA revealed two prominent protein bands that corresponded to the expected sizes of alternative translation. A novel fifth motif, identified as critical for the glycerolphosphate acyltransferase family, was observed in human tafazzin. The presence of a mutation in this region in Barth syndrome patients indicates that this motif is essential for tafazzin function.
Rosalind A. Coleman - One of the best experts on this subject based on the ideXlab platform.
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Complex Expression pattern of the barth syndrome gene product tafazzin in human cell lines and murine tissues
Biochemistry and Cell Biology, 2004Co-Authors: Biao Lu, Marguerite R Kelher, Tal M Lewin, Rosalind A. Coleman, Patrick C. Choy, Grant M HatchAbstract:Tafazzins, a group of proteins that are defective in patients with Barth syndrome, are produced by alternate splicing of the gene G4.5 or TAZ. RT-PCR and transcription-coupled in vitro translation analysis were undertaken to determine the Expression of alternatively spliced TAZ mRNA in mouse tissues and human cell lines. Only two tafazzin transcripts, both lacking exon 5, were expressed in murine tissues, whereas four tafazzin transcripts, all lacking exon 5, were observed in human umbilical vein vascular endothelial cells and U937 human monoblasts indicating a species-specific difference in the Expression of TAZ mRNAs in mouse and humans. Only TAZ lacking exon 5 was expressed in murine heart. Differentiation of U937 human monoblasts into macrophages did not alter Expression of the tafazzin transcripts indicating that TAZ Expression is independent of monocyte differentiation. Cloning and in vitro Expression of both murine and human tafazzin cDNA revealed two prominent protein bands that corresponded to th...
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Complex Expression pattern of the Barth syndrome gene product tafazzin in human cell lines and murine tissues.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 2004Co-Authors: Marguerite R Kelher, Tal M Lewin, Rosalind A. Coleman, Patrick C. Choy, Douglas P Lee, Grant M HatchAbstract:Tafazzins, a group of proteins that are defective in patients with Barth syndrome, are produced by alternate splicing of the gene G4.5 or TAZ. RT-PCR and transcription-coupled in vitro translation ...
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Complex Expression pattern of the barth syndrome gene product tafazzin in human cell lines and murine tissues
Biochemistry and Cell Biology, 2004Co-Authors: Marguerite R Kelher, Tal M Lewin, Rosalind A. Coleman, Patrick C. Choy, Douglas P Lee, Grant M HatchAbstract:Tafazzins, a group of proteins that are defective in patients with Barth syndrome, are produced by alternate splicing of the gene G4.5 or TAZ. RT-PCR and transcription-coupled in vitro translation analysis were undertaken to determine the Expression of alternatively spliced TAZ mRNA in mouse tissues and human cell lines. Only two tafazzin transcripts, both lacking exon 5, were expressed in murine tissues, whereas four tafazzin transcripts, all lacking exon 5, were observed in human umbilical vein vascular endothelial cells and U937 human monoblasts indicating a species-specific difference in the Expression of TAZ mRNAs in mouse and humans. Only TAZ lacking exon 5 was expressed in murine heart. Differentiation of U937 human monoblasts into macrophages did not alter Expression of the tafazzin transcripts indicating that TAZ Expression is independent of monocyte differentiation. Cloning and in vitro Expression of both murine and human tafazzin cDNA revealed two prominent protein bands that corresponded to the expected sizes of alternative translation. A novel fifth motif, identified as critical for the glycerolphosphate acyltransferase family, was observed in human tafazzin. The presence of a mutation in this region in Barth syndrome patients indicates that this motif is essential for tafazzin function.