The Experts below are selected from a list of 104598 Experts worldwide ranked by ideXlab platform

David H. Ingbar - One of the best experts on this subject based on the ideXlab platform.

Pubudu P Handakumbura - One of the best experts on this subject based on the ideXlab platform.

  • an arabidopsis gene regulatory network for secondary cell wall synthesis
    Nature, 2015
    Co-Authors: Mallorie Taylorteeples, M De Lucas, Gina M Turco, Ted Toal, Allison Gaudinier, N F Young, Gina M Trabucco, Mike T Veling, R Lamothe, Pubudu P Handakumbura
    Abstract:

    The plant cell wall is an important factor for determining cell shape, function and response to the environment. Secondary cell walls, such as those found in xylem, are composed of cellulose, hemicelluloses and lignin and account for the bulk of plant biomass. The coordination between transcriptional regulation of synthesis for each polymer is Complex and vital to cell function. A regulatory hierarchy of developmental switches has been proposed, although the full complement of regulators remains unknown. Here we present a protein-DNA network between Arabidopsis thaliana transcription factors and secondary cell wall metabolic genes with gene expression regulated by a series of feed-forward loops. This model allowed us to develop and validate new hypotheses about secondary wall gene regulation under abiotic stress. Distinct stresses are able to perturb targeted genes to potentially promote functional adaptation. These interactions will serve as a foundation for understanding the regulation of a Complex, Integral plant component.

Semyon Alesker - One of the best experts on this subject based on the ideXlab platform.

Shikha Nangia - One of the best experts on this subject based on the ideXlab platform.

  • the ghrelin o acyltransferase structure reveals a catalytic channel for transmembrane hormone acylation
    Journal of Biological Chemistry, 2019
    Co-Authors: Maria B Campana, Flaviyan Jerome Irudayanathan, Tasha R Davis, Kayleigh R Mcgoverngooch, Rosemary Loftus, Mohammad Ashkar, Najae Escoffery, Melissa Navarro, Michelle A Sieburg, Shikha Nangia
    Abstract:

    Integral membrane proteins represent a large and diverse portion of the proteome and are often recalcitrant to purification, impeding studies essential for understanding protein structure and function. By combining co-evolutionary constraints and computational modeling with biochemical validation through site-directed mutagenesis and enzyme activity assays, we demonstrate here a synergistic approach to structurally model purification-resistant topologically Complex Integral membrane proteins. We report the first structural model of a eukaryotic membrane-bound O-acyltransferase (MBOAT), ghrelin O-acyltransferase (GOAT), which modifies the metabolism-regulating hormone ghrelin. Our structure, generated in the absence of any experimental structural data, revealed an unanticipated strategy for transmembrane protein acylation with catalysis occurring in an internal channel connecting the endoplasmic reticulum lumen and cytoplasm. This finding validated the power of our approach to generate predictive structural models for other experimentally challenging Integral membrane proteins. Our results illuminate novel aspects of membrane protein function and represent key steps for advancing structure-guided inhibitor design to target therapeutically important but experimentally intractable membrane proteins.

Jianxun Lei - One of the best experts on this subject based on the ideXlab platform.