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Andreas Brauninger - One of the best experts on this subject based on the ideXlab platform.

  • analysis of a clonally related mantle cell and hodgkin Lymphoma indicates epstein barr virus infection of a hodgkin reed sternberg cell precursor in a germinal center
    The American Journal of Surgical Pathology, 2003
    Co-Authors: Marianne Tinguely, Ralf Kuppers, Martinleo Hansmann, Richard Rosenquist, Christer Sundstrom, Rosemarie Amini, Andreas Brauninger
    Abstract:

    The simultaneous occurrence of a Hodgkin Lymphoma (HL) and a non-Hodgkin Lymphoma (NHL) is a rare event, and single cell analyses of such Composite Lymphomas revealed that NHL and Hodgkin/Reed-Sternberg (HRS) tumor cells are frequently descendants of the same tumor clone precursors. Here we present a Composite Lymphoma consisting of a mantle cell Lymphoma (MCL) and an HL with EBV- and EBV+ HRS cells. Analysis of rearranged V genes of single cells revealed a clonal relationship between MCL and HL tumor cells. Although V gene rearrangements of the MCL were unmutated, mutations were observed in HRS cells. Besides mutations shared by all HRS cells, the EBV+ HRS cells carried identical additional mutations. These findings show that both Lymphomas derive from a common precursor, most likely a pre germinal center (GC) B cell that already carried some transforming event(s). However, the presence of mutations in the V genes of the HRS cells further corroborates the importance of the GC reaction for the pathogenesis of HL. Importantly, the finding that only a subclone of the HRS clone, defined by a particular mutation pattern, was EBV infected represents a strong indication that EBV infection of the HRS cell precursor happened in the GC.

  • identification of common germinal center b cell precursors in two patients with both hodgkin s disease and non hodgkin s Lymphoma
    The New England Journal of Medicine, 1999
    Co-Authors: Andreas Brauninger, Martinleo Hansmann, John G Strickler, Reinhard Dummer, G Burg, Klaus Rajewsky, Ralf Kuppers
    Abstract:

    Background Hodgkin's disease and non-Hodgkin's B-cell Lymphoma occasionally occur in the same patient. The identification of a common precursor of the two types of Lymphoma would show definitively that Reed–Sternberg cells originate from B cells. Methods We studied Lymphomas from two patients, one with a Composite Lymphoma (classic Hodgkin's disease and a follicular Lymphoma in the same lymph node) and the other with a T-cell–rich B-cell Lymphoma that was followed by classic Hodgkin's disease. Single Reed–Sternberg cells and non-Hodgkin's Lymphoma cells from frozen sections were micromanipulated. The rearranged immunoglobulin variable-region genes (V genes) of the heavy and light chains were amplified by the polymerase chain reaction from genomic DNA and sequenced. Results In both patients, the Reed–Sternberg cells were related clonally to the non-Hodgkin's Lymphoma B cells. The V genes carried somatic mutations (a hallmark of germinal-center B cells and their descendants). In both patients, some somatic ...

Rocha Filho,francisco Dário - One of the best experts on this subject based on the ideXlab platform.

  • Simultaneous occurrence of follicular Lymphoma and mixed-cellularity Hodgkin's Lymphoma: lymph node and extranodal involvement
    Sociedade Brasileira de Citopatologia, 2004
    Co-Authors: Grangeiro,maria Do Patrocínio F., Magalhães,sílvia Maria M., Ferreira,francisco Valdeci A., Rocha Filho,francisco Dário
    Abstract:

    An unusual and well-characterised case of Composite Lymphoma in the spleen and lymph node is presented. The simultaneous occurrence of mixed-cellularity Hodgkin's Lymphoma (HL) and follicular non-Hodgkin's Lymphoma (NHL) was demonstrated in a 66-year-old man admitted in our Service with anaemia, hepatosplenomegaly and multiple abdominal lymph nodes. The morphological study of the spleen and lymph node of the splenic hilum showed an infiltrate composed of two distinct neoplasias. The liver was involved by NHL infiltrate and the peripancreatic lymph node exhibited HL. The Reed-Sternberg (RS) cells expressed CD 15 and CD 30, whereas the NHL cells presented standard immunohistochemical features of follicular Lymphoma. To our knowledge, this is the fifth case report of concurrent spleen involvement by Composite Lymphoma. The incidence, clinicopathological and immunohistochemical features of this rare association are discussed

  • Ocorrência simultânea de linfoma folicular e linfoma de Hodgkin celularidade mista: envolvimento nodal e extranodal
    Sociedade Brasileira de Citopatologia, 2004
    Co-Authors: Grangeiro,maria Do Patrocínio F., Magalhães,sílvia Maria M., Ferreira,francisco Valdeci A., Rocha Filho,francisco Dário
    Abstract:

    An unusual and well-characterised case of Composite Lymphoma in the spleen and lymph node is presented. The simultaneous occurrence of mixed-cellularity Hodgkin's Lymphoma (HL) and follicular non-Hodgkin's Lymphoma (NHL) was demonstrated in a 66-year-old man admitted in our Service with anaemia, hepatosplenomegaly and multiple abdominal lymph nodes. The morphological study of the spleen and lymph node of the splenic hilum showed an infiltrate composed of two distinct neoplasias. The liver was involved by NHL infiltrate and the peripancreatic lymph node exhibited HL. The Reed-Sternberg (RS) cells expressed CD 15 and CD 30, whereas the NHL cells presented standard immunohistochemical features of follicular Lymphoma. To our knowledge, this is the fifth case report of concurrent spleen involvement by Composite Lymphoma. The incidence, clinicopathological and immunohistochemical features of this rare association are discussed.Os autores apresentam um caso raro e bem caracterizado de linfoma composto no baço e linfonodos. A ocorrência simultânea de um linfoma de Hodgkin e um linfoma não-Hodgkin foi demonstrada em um homem de 66 anos admitido com anemia, hepatosplenomegalia e múltiplos linfonodos abdominais. O estudo morfológico do baço e linfonodo do hilo esplênico mostrou infiltração por duas neoplasias distintas. O fígado estava envolvido por linfoma não-Hodgkin e o linfonodo peripancreático mostrava um linfoma de Hodgkin. As células de Reed-Sternberg (RS) expressaram CD 15 e CD 30, enquanto as células do linfoma não-Hodgkin apresentaram os aspectos imunoistoquímicos clássicos do linfoma folicular. Na literatura médica, este é o quinto caso descrito de envolvimento do baço por linfoma composto. A incidência, aspectos clínicos e imunoistoquímicos desta rara associação são discutidos

Ralf Kuppers - One of the best experts on this subject based on the ideXlab platform.

  • analysis of a clonally related mantle cell and hodgkin Lymphoma indicates epstein barr virus infection of a hodgkin reed sternberg cell precursor in a germinal center
    The American Journal of Surgical Pathology, 2003
    Co-Authors: Marianne Tinguely, Ralf Kuppers, Martinleo Hansmann, Richard Rosenquist, Christer Sundstrom, Rosemarie Amini, Andreas Brauninger
    Abstract:

    The simultaneous occurrence of a Hodgkin Lymphoma (HL) and a non-Hodgkin Lymphoma (NHL) is a rare event, and single cell analyses of such Composite Lymphomas revealed that NHL and Hodgkin/Reed-Sternberg (HRS) tumor cells are frequently descendants of the same tumor clone precursors. Here we present a Composite Lymphoma consisting of a mantle cell Lymphoma (MCL) and an HL with EBV- and EBV+ HRS cells. Analysis of rearranged V genes of single cells revealed a clonal relationship between MCL and HL tumor cells. Although V gene rearrangements of the MCL were unmutated, mutations were observed in HRS cells. Besides mutations shared by all HRS cells, the EBV+ HRS cells carried identical additional mutations. These findings show that both Lymphomas derive from a common precursor, most likely a pre germinal center (GC) B cell that already carried some transforming event(s). However, the presence of mutations in the V genes of the HRS cells further corroborates the importance of the GC reaction for the pathogenesis of HL. Importantly, the finding that only a subclone of the HRS clone, defined by a particular mutation pattern, was EBV infected represents a strong indication that EBV infection of the HRS cell precursor happened in the GC.

  • identification of common germinal center b cell precursors in two patients with both hodgkin s disease and non hodgkin s Lymphoma
    The New England Journal of Medicine, 1999
    Co-Authors: Andreas Brauninger, Martinleo Hansmann, John G Strickler, Reinhard Dummer, G Burg, Klaus Rajewsky, Ralf Kuppers
    Abstract:

    Background Hodgkin's disease and non-Hodgkin's B-cell Lymphoma occasionally occur in the same patient. The identification of a common precursor of the two types of Lymphoma would show definitively that Reed–Sternberg cells originate from B cells. Methods We studied Lymphomas from two patients, one with a Composite Lymphoma (classic Hodgkin's disease and a follicular Lymphoma in the same lymph node) and the other with a T-cell–rich B-cell Lymphoma that was followed by classic Hodgkin's disease. Single Reed–Sternberg cells and non-Hodgkin's Lymphoma cells from frozen sections were micromanipulated. The rearranged immunoglobulin variable-region genes (V genes) of the heavy and light chains were amplified by the polymerase chain reaction from genomic DNA and sequenced. Results In both patients, the Reed–Sternberg cells were related clonally to the non-Hodgkin's Lymphoma B cells. The V genes carried somatic mutations (a hallmark of germinal-center B cells and their descendants). In both patients, some somatic ...

Peter G Isaacson - One of the best experts on this subject based on the ideXlab platform.

  • Composite mantle cell Lymphoma and plasmacytoma
    Human Pathology, 1997
    Co-Authors: Adrian R Cachia, T C Diss, Peter G Isaacson
    Abstract:

    We report a case of a Composite Lymphoma comprising both mantle cell Lymphoma and a plasmacytoma. The two components were morphologically and immunohistochemically quite distinct. These properties, coupled with both direct and indirect molecular genetic evidence, suggest that these were two separate tumours occurring together by chance or by unknown oncogenic mechansisms, rather than clonally linked Lymphomas.

Silvia Uccella - One of the best experts on this subject based on the ideXlab platform.

  • Composite follicular Lymphoma and early in situ and mantle zone growth pattern mantle cell neoplasia a rare entity with peculiar cytogenetic and clinical features
    Pathology Research and Practice, 2020
    Co-Authors: Lisa F Vivian, Francesca Magnoli, Leonardo Campiotti, Claudio Chini, Giuseppe Calabrese, Fausto Sessa, Maria Grazia Tibiletti, Silvia Uccella
    Abstract:

    Composite follicular Lymphoma (FL) and mantle cell Lymphoma (MCL) is rare and not fully characterized from a genetic and clinicopathological point of view. We report a Composite Lymphoma (CL) in which a G1-2 FL was associated with an in situ mantle cell neoplasia (ISMCN) and a mantle zone growth pattern (MZGP) MCL, followed-up for six years after the first diagnosis, until the exitus of the patient. We performed a comprehensive immunohistochemical study and a detailed cytogenetic analysis, including conventional karyotyping, SKY FISH, FISH on metaphases and interphasic separated nuclei, and FISH on histological sections. The study was completed by the review of the 13 published Composite FL and MCL. Our results show that this entity generally behaves like an indolent Lymphoma, with the outcome of patients driven by the progression of the FL component. The MCL component generally does not evolve in an aggressive disease. Indeed, half of the cases present exclusively ISMCN. In our case, mantle cell neoplasia at diagnosis was represented by ISMCN and MZGP MCL and it was characterized by a simple karyotype, with t(11;14) as the sole cytogenetic abnormality. This cytogenetic aspect well correlates with the indolent behavior of the mantle cell component. Conversely, the complex karyotype of the FL component was associated with disseminated disease that influenced patient's outcome. Finally, we suggest that not only ISMCN, but also isolated MZGP MCL, may be considered as lesions with low potential of transformation in an aggressive MCL.