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Wibool Piyawattanametha - One of the best experts on this subject based on the ideXlab platform.

  • Optical Fiber-Based Laser Confocal Microscope with a Metalens
    2018 International Conference on Optical MEMS and Nanophotonics (OMN), 2018
    Co-Authors: Qiu Zhen, Daniel Lopez, Haogang Cai, Wibool Piyawattanametha
    Abstract:

    We demonstrated a fiber optical based laser Confocal Microscope with a planar metalens as a focusing element. The metalens is used to replace a bulky traditional focusing lens in a standard Confocal Microscope configuration. The metalens $(\text{f}=4\ \text{mm})$ is designed to operate at 660 mm with a measured $4.29 \mu\text{m}$ FWHM spot size.

  • Handheld multispectral dual-axis Confocal Microscope for cervical cancer screening
    Endoscopic Microscopy VIII, 2013
    Co-Authors: Pongsak Sarapukdee, Santi Rattanavarin, Ungkarn Jarujareet, Numfon Khemthongcharoen, Michael J. Mandella, Daniel Lopez, Romuald Jolivot, Il Woong Jung, Wibool Piyawattanametha
    Abstract:

    Our work demonstrates a MEMS based handheld dual-axis Confocal Microscope for cervical cancer screening. Imaging demonstration is performed with plant and animal tissue biopsies. The data is collected and displayed in real time with 2-5 Hz frame rates.

  • MEMS-based handheld dual-axis Confocal Microscope for cervix cancer screening
    2012 9th International Conference on Electrical Engineering Electronics Computer Telecommunications and Information Technology, 2012
    Co-Authors: Pongsak Sarapukdee, Santi Rattanavarin, Ungkarn Jarujareet, Numfon Khemthongcharoen, Athisake Ruangphacha, Michael J. Mandella, Wibool Piyawattanametha
    Abstract:

    Our work demonstrated a MEMS based handheld dual-axis Confocal Microscope for cervical cancer screening. Imaging demonstration is performed with plants and animal tissue biopsies. The data is collected and displayed in real time with 2-5 Hz.

  • Surgical dual-axis Confocal Microscope for brain tumor resection
    2010 IEEE Photonics Society Winter Topicals Meeting Series (WTM), 2010
    Co-Authors: Jonathan T. C. Liu, Wibool Piyawattanametha, Michael J. Mandella, Nathan O Loewke, Henry Haeberle, Olav Solgaard, G S Kino, Christopher H Contag
    Abstract:

    A 1.8-mm diameter gradient-index relay lens has been incorporated into a miniature dual-axis Confocal Microscope for image-guided surgery. A biaxial MEMS mirror is actuated at resonance along each axis to create a non-repeating Lissajous scan pattern for achieving large fields of view.

  • 3 d near infrared fluorescence imaging using an mems based miniature dual axis Confocal Microscope
    IEEE Journal of Selected Topics in Quantum Electronics, 2009
    Co-Authors: Wibool Piyawattanametha, Michael J. Mandella, Olav Solgaard, G S Kino, Kevin E Loewke, Thomas D Wang, Christopher H Contag
    Abstract:

    We demonstrate a fast miniature microelectro-mechanical-system-based near-infrared fluorescence dual-axis Confocal Microscope in a 10-mm-diameter package for 3-D imaging in both ex vivo and in vivo samples. The miniature Microscope, while in contact with the targeted tissue, can reveal subsurface structure or anatomy as deep as 300 mum. The lateral and axial resolutions are 5 and 7 mum, respectively. Real-time en face mosaicing image of in vivo human skin is demonstrated to enlarge the overall FOV to be over 3 mm at acquisition frame rate of 5 frames/s.

Thomas D Wang - One of the best experts on this subject based on the ideXlab platform.

  • 3 d near infrared fluorescence imaging using an mems based miniature dual axis Confocal Microscope
    IEEE Journal of Selected Topics in Quantum Electronics, 2009
    Co-Authors: Wibool Piyawattanametha, Michael J. Mandella, Olav Solgaard, G S Kino, Kevin E Loewke, Thomas D Wang, Christopher H Contag
    Abstract:

    We demonstrate a fast miniature microelectro-mechanical-system-based near-infrared fluorescence dual-axis Confocal Microscope in a 10-mm-diameter package for 3-D imaging in both ex vivo and in vivo samples. The miniature Microscope, while in contact with the targeted tissue, can reveal subsurface structure or anatomy as deep as 300 mum. The lateral and axial resolutions are 5 and 7 mum, respectively. Real-time en face mosaicing image of in vivo human skin is demonstrated to enlarge the overall FOV to be over 3 mm at acquisition frame rate of 5 frames/s.

  • three dimensional in vivo imaging by a handheld dual axes Confocal Microscope
    Optics Express, 2008
    Co-Authors: Wibool Piyawattanametha, Michael J. Mandella, G S Kino, Christopher H Contag, Thomas D Wang, Pei Lin Hsiung, Jonathan Hardy, Olav Solgaard
    Abstract:

    We present a handheld dual-axes Confocal Microscope that is based on a two-dimensional microelectromechanical systems (MEMS) scanner. It performs reflectance and fluorescence imaging at 488 nm wavelength, with three-dimensional imaging capability. The fully packaged Microscope has a diameter of 10 mm and acquires images at 4 Hz frame rate with a maximum field of view of 400 µm×260 µm. The transverse and axial resolutions of the handheld probe are 1.7 µm and 5.8 µm, respectively. Capability to perform real time small animal imaging is demonstrated in vivo in transgenic mice.

  • miniature near infrared dual axes Confocal Microscope utilizing a two dimensional microelectromechanical systems scanner
    Optics Letters, 2007
    Co-Authors: Jonathan T. C. Liu, Wibool Piyawattanametha, Michael J. Mandella, Olav Solgaard, G S Kino, Christopher H Contag, Larry K Wong, Thomas D Wang
    Abstract:

    The first, to our knowledge, miniature dual-axes Confocal Microscope has been developed, with an outer diameter of 10 mm, for subsurface imaging of biological tissues with 5-7 μm resolution. Depth-resolved en face images are obtained at 30 frames per second, with a field of view of 800×100 μm, by employing a two-dimensional scanning microelectromechanical systems mirror. Reflectance and fluorescence images are obtained with a laser source at 785 nm, demonstrating the ability to perform real-time optical biopsy.

  • dual axis Confocal Microscope for high resolution in vivo imaging
    Optics Letters, 2003
    Co-Authors: Thomas D Wang, Michael J. Mandella, Christopher H Contag, G S Kino
    Abstract:

    We describe a novel Confocal Microscope that uses separate low-numerical-aperture objectives with the illumination and collection axes crossed at angle θ from the midline. This architecture collects images in scattering media with high transverse and axial resolution, long working distance, large field of view, and reduced noise from scattered light. We measured transverse and axial (FWHM) resolution of 1.3 and 2.1 μm, respectively, in free space, and confirm subcellular resolution in excised esophageal mucosa. The optics may be scaled to millimeter dimensions and fiber coupled for collection of high-resolution images in vivo.

C. F. Kaminski - One of the best experts on this subject based on the ideXlab platform.

  • A white light Confocal Microscope for spectrally resolved multidimensional imaging
    Journal of Microscopy, 2007
    Co-Authors: J H Frank, A. D. Elder, Anand D. Jeyasekharan, Johannes Swartling, Ashok R. Venkitaraman, C. F. Kaminski
    Abstract:

    Spectrofluorometric imaging microscopy is demonstrated in a Confocal Microscope using a supercontinuum laser as an excitation source and a custom-built prism spectrometer for detection. This Microscope system provides Confocal imaging with spectrally resolved fluorescence excitation and detection from 450 to 700 nm. The supercontinuum laser provides a broad spectrum light source and is coupled with an acousto-optic tunable filter to provide continuously tunable fluorescence excitation with a 1-nm bandwidth. Eight different excitation wavelengths can be simultaneously selected. The prism spectrometer provides spectrally resolved detection with sensitivity comparable to a standard Confocal system. This new Microscope system enables optimal access to a multitude of fluorophores and provides fluorescence excitation and emission spectra for each location in a 3D Confocal image. The speed of the spectral scans is suitable for spectrofluorometric imaging of live cells. Effects of chromatic aberration are modest and do not significantly limit the spatial resolution of the Confocal measurements.

Tony Wilson - One of the best experts on this subject based on the ideXlab platform.

  • Resolution and optical sectioning in the Confocal Microscope
    Journal of microscopy, 2011
    Co-Authors: Tony Wilson
    Abstract:

    Summary In this short review, we present a self-contained discussion of the image formation properties of the fluorescent Confocal Microscope. The optical sectioning or depth discrimination property is discussed in detail and new analytic formulae are presented, which relate the optical sectioning strength to the wavelength, numerical aperture and pinhole aperture size in a particularly simple fashion.

  • Adaptive aberration correction in a Confocal Microscope
    Proceedings of the National Academy of Sciences of the United States of America, 2002
    Co-Authors: Martin J. Booth, Mark A. A. Neil, Rimas Juskaitis, Tony Wilson
    Abstract:

    The main advantage of Confocal Microscopes over their conventional counterparts is their ability to optically "section" thick specimens; the thin image slices thus obtained can be used to reconstruct three-dimensional images, a capability which is particularly useful in biological applications. However, it is well known that the resolution and optical sectioning ability can be severely degraded by system or specimen-induced aberrations. The use of high aperture lenses further exacerbates the problem. Moreover, aberrations can considerably reduce the number of photons that reach the detector, leading to lower contrast. It is rather unfortunate, therefore, that in practical microscopy, aberration-free Confocal imaging is rarely achieved. Adaptive optics systems, which have been used widely to correct aberrations in astronomy, offer a solution here but also present new challenges. The optical system and the source of aberrations in a Confocal Microscope are considerably different and require a novel approach to wavefront sensing. This method, based upon direct measurement of Zernike aberration modes, also exhibits an axial selectivity similar to that of a Confocal Microscope. We demonstrate an adaptive Confocal fluorescence Microscope incorporating this modal sensor together with a deformable membrane mirror for aberration correction. Aberration corrected images of biological specimens show considerable improvement in contrast and apparent restoration of axial resolution.

G S Kino - One of the best experts on this subject based on the ideXlab platform.

  • Surgical dual-axis Confocal Microscope for brain tumor resection
    2010 IEEE Photonics Society Winter Topicals Meeting Series (WTM), 2010
    Co-Authors: Jonathan T. C. Liu, Wibool Piyawattanametha, Michael J. Mandella, Nathan O Loewke, Henry Haeberle, Olav Solgaard, G S Kino, Christopher H Contag
    Abstract:

    A 1.8-mm diameter gradient-index relay lens has been incorporated into a miniature dual-axis Confocal Microscope for image-guided surgery. A biaxial MEMS mirror is actuated at resonance along each axis to create a non-repeating Lissajous scan pattern for achieving large fields of view.

  • 3 d near infrared fluorescence imaging using an mems based miniature dual axis Confocal Microscope
    IEEE Journal of Selected Topics in Quantum Electronics, 2009
    Co-Authors: Wibool Piyawattanametha, Michael J. Mandella, Olav Solgaard, G S Kino, Kevin E Loewke, Thomas D Wang, Christopher H Contag
    Abstract:

    We demonstrate a fast miniature microelectro-mechanical-system-based near-infrared fluorescence dual-axis Confocal Microscope in a 10-mm-diameter package for 3-D imaging in both ex vivo and in vivo samples. The miniature Microscope, while in contact with the targeted tissue, can reveal subsurface structure or anatomy as deep as 300 mum. The lateral and axial resolutions are 5 and 7 mum, respectively. Real-time en face mosaicing image of in vivo human skin is demonstrated to enlarge the overall FOV to be over 3 mm at acquisition frame rate of 5 frames/s.

  • Sequential in vivo molecular imaging with a dual-axes Confocal Microscope
    Conference on Lasers and Electro-Optics International Quantum Electronics Conference, 2009
    Co-Authors: E. Gonzalez, Wibool Piyawattanametha, Michael J. Mandella, G S Kino, Christopher H Contag, Roger L. Kaspar, Olav Solgaard
    Abstract:

    We demonstrate sequential three-dimensional in vivo imaging in one mouse, monitoring siRNA silencing with a dual-axes Confocal Microscope. This opens up new possibilities in developing therapeutics for genetic disorders through better assessment of treatment efficiency.

  • three dimensional in vivo imaging by a handheld dual axes Confocal Microscope
    Optics Express, 2008
    Co-Authors: Wibool Piyawattanametha, Michael J. Mandella, G S Kino, Christopher H Contag, Thomas D Wang, Pei Lin Hsiung, Jonathan Hardy, Olav Solgaard
    Abstract:

    We present a handheld dual-axes Confocal Microscope that is based on a two-dimensional microelectromechanical systems (MEMS) scanner. It performs reflectance and fluorescence imaging at 488 nm wavelength, with three-dimensional imaging capability. The fully packaged Microscope has a diameter of 10 mm and acquires images at 4 Hz frame rate with a maximum field of view of 400 µm×260 µm. The transverse and axial resolutions of the handheld probe are 1.7 µm and 5.8 µm, respectively. Capability to perform real time small animal imaging is demonstrated in vivo in transgenic mice.

  • miniature near infrared dual axes Confocal Microscope utilizing a two dimensional microelectromechanical systems scanner
    Optics Letters, 2007
    Co-Authors: Jonathan T. C. Liu, Wibool Piyawattanametha, Michael J. Mandella, Olav Solgaard, G S Kino, Christopher H Contag, Larry K Wong, Thomas D Wang
    Abstract:

    The first, to our knowledge, miniature dual-axes Confocal Microscope has been developed, with an outer diameter of 10 mm, for subsurface imaging of biological tissues with 5-7 μm resolution. Depth-resolved en face images are obtained at 30 frames per second, with a field of view of 800×100 μm, by employing a two-dimensional scanning microelectromechanical systems mirror. Reflectance and fluorescence images are obtained with a laser source at 785 nm, demonstrating the ability to perform real-time optical biopsy.