The Experts below are selected from a list of 798 Experts worldwide ranked by ideXlab platform

Keiko Ikeda - One of the best experts on this subject based on the ideXlab platform.

  • RESEARCH ARTICLE A Phox2b BAC Transgenic Rat Line Useful for Understanding Respiratory Rhythm Generator Neural Circuitry
    2016
    Co-Authors: Keiko Ikeda, Masanori Takahashi, Shigeru Sato, Hiroyuki Igarashi, Toru Ishizuka, Hiromu Yawo, Satoru Arata, Michelle E. Southard-smith, Kiyoshi Kawakami
    Abstract:

    The key role of the respiratory neural center is respiratory rhythm generation to maintain homeostasis through the control of arterial blood pCO2/pH and pO2 levels. The neuronal network responsible for respiratory rhythm generation in neonatal rat resides in the ventral side of the medulla and is composed of two groups; the parafacial respiratory group (pFRG) and the pre-Bötzinger complex group (preBötC). The pFRG partially overlaps in the retrotra-pezoid nucleus (RTN), which was originally identified in adult cats and rats. Part of the pre-inspiratory (Pre-I) neurons in the RTN/pFRG serves as central chemoreceptor neurons and the CO2 sensitive Pre-I neurons express homeobox gene Phox2b. Phox2b encodes a tran-scription factor and is essential for the development of the sensory-motor visceral circuits. Mutations in human PHOX2B cause Congenital Hypoventilation Syndrome, which is charac-terized by blunted ventilatory response to hypercapnia. Here we describe the generation of a novel transgenic (Tg) rat harboring fluorescently labeled Pre-I neurons in the RTN/pFRG. In addition, the Tg rat showed fluorescent signals in autonomic enteric neurons and carotid bodies. Because the Tg rat expresses inducible Cre recombinase in PHOX2B-positive cell

  • a phox2b bac transgenic rat line useful for understanding respiratory rhythm generator neural circuitry
    PLOS ONE, 2015
    Co-Authors: Keiko Ikeda, Masanori Takahashi, Shigeru Sato, Hiroyuki Igarashi, Toru Ishizuka, Hiromu Yawo, Satoru Arata, Michelle E Southardsmith, Kiyoshi Kawakami
    Abstract:

    The key role of the respiratory neural center is respiratory rhythm generation to maintain homeostasis through the control of arterial blood pCO2/pH and pO2 levels. The neuronal network responsible for respiratory rhythm generation in neonatal rat resides in the ventral side of the medulla and is composed of two groups; the parafacial respiratory group (pFRG) and the pre-Botzinger complex group (preBotC). The pFRG partially overlaps in the retrotrapezoid nucleus (RTN), which was originally identified in adult cats and rats. Part of the pre-inspiratory (Pre-I) neurons in the RTN/pFRG serves as central chemoreceptor neurons and the CO2 sensitive Pre-I neurons express homeobox gene Phox2b. Phox2b encodes a transcription factor and is essential for the development of the sensory-motor visceral circuits. Mutations in human PHOX2B cause Congenital Hypoventilation Syndrome, which is characterized by blunted ventilatory response to hypercapnia. Here we describe the generation of a novel transgenic (Tg) rat harboring fluorescently labeled Pre-I neurons in the RTN/pFRG. In addition, the Tg rat showed fluorescent signals in autonomic enteric neurons and carotid bodies. Because the Tg rat expresses inducible Cre recombinase in PHOX2B-positive cells during development, it is a potentially powerful tool for dissecting the entire picture of the respiratory neural network during development and for identifying the CO2/O2 sensor molecules in the adult central and peripheral nervous systems.

Kiyoshi Kawakami - One of the best experts on this subject based on the ideXlab platform.

  • RESEARCH ARTICLE A Phox2b BAC Transgenic Rat Line Useful for Understanding Respiratory Rhythm Generator Neural Circuitry
    2016
    Co-Authors: Keiko Ikeda, Masanori Takahashi, Shigeru Sato, Hiroyuki Igarashi, Toru Ishizuka, Hiromu Yawo, Satoru Arata, Michelle E. Southard-smith, Kiyoshi Kawakami
    Abstract:

    The key role of the respiratory neural center is respiratory rhythm generation to maintain homeostasis through the control of arterial blood pCO2/pH and pO2 levels. The neuronal network responsible for respiratory rhythm generation in neonatal rat resides in the ventral side of the medulla and is composed of two groups; the parafacial respiratory group (pFRG) and the pre-Bötzinger complex group (preBötC). The pFRG partially overlaps in the retrotra-pezoid nucleus (RTN), which was originally identified in adult cats and rats. Part of the pre-inspiratory (Pre-I) neurons in the RTN/pFRG serves as central chemoreceptor neurons and the CO2 sensitive Pre-I neurons express homeobox gene Phox2b. Phox2b encodes a tran-scription factor and is essential for the development of the sensory-motor visceral circuits. Mutations in human PHOX2B cause Congenital Hypoventilation Syndrome, which is charac-terized by blunted ventilatory response to hypercapnia. Here we describe the generation of a novel transgenic (Tg) rat harboring fluorescently labeled Pre-I neurons in the RTN/pFRG. In addition, the Tg rat showed fluorescent signals in autonomic enteric neurons and carotid bodies. Because the Tg rat expresses inducible Cre recombinase in PHOX2B-positive cell

  • a phox2b bac transgenic rat line useful for understanding respiratory rhythm generator neural circuitry
    PLOS ONE, 2015
    Co-Authors: Keiko Ikeda, Masanori Takahashi, Shigeru Sato, Hiroyuki Igarashi, Toru Ishizuka, Hiromu Yawo, Satoru Arata, Michelle E Southardsmith, Kiyoshi Kawakami
    Abstract:

    The key role of the respiratory neural center is respiratory rhythm generation to maintain homeostasis through the control of arterial blood pCO2/pH and pO2 levels. The neuronal network responsible for respiratory rhythm generation in neonatal rat resides in the ventral side of the medulla and is composed of two groups; the parafacial respiratory group (pFRG) and the pre-Botzinger complex group (preBotC). The pFRG partially overlaps in the retrotrapezoid nucleus (RTN), which was originally identified in adult cats and rats. Part of the pre-inspiratory (Pre-I) neurons in the RTN/pFRG serves as central chemoreceptor neurons and the CO2 sensitive Pre-I neurons express homeobox gene Phox2b. Phox2b encodes a transcription factor and is essential for the development of the sensory-motor visceral circuits. Mutations in human PHOX2B cause Congenital Hypoventilation Syndrome, which is characterized by blunted ventilatory response to hypercapnia. Here we describe the generation of a novel transgenic (Tg) rat harboring fluorescently labeled Pre-I neurons in the RTN/pFRG. In addition, the Tg rat showed fluorescent signals in autonomic enteric neurons and carotid bodies. Because the Tg rat expresses inducible Cre recombinase in PHOX2B-positive cells during development, it is a potentially powerful tool for dissecting the entire picture of the respiratory neural network during development and for identifying the CO2/O2 sensor molecules in the adult central and peripheral nervous systems.

Christo Goridis - One of the best experts on this subject based on the ideXlab platform.

  • PHOX2B in respiratory control: lessons from Congenital central Hypoventilation Syndrome and its mouse models.
    Respiratory physiology & neurobiology, 2009
    Co-Authors: Jeanne Amiel, Nelina Ramanantsoa, Gilles Fortin, Veronique Dubreuil, Jorge Gallego, Jeanfrancois Brunet, Christo Goridis
    Abstract:

    Phox2b is a master regulator of visceral reflex circuits. Its role in the control of respiration has been highlighted by the identification of heterozygous PHOX2B mutations as the cause of Central Congenital Hypoventilation Syndrome (CCHS), a rare disease defined by the lack of CO(2) responsiveness and of breathing automaticity in sleep. Phox2b(27Ala/+) mice that bear a frequent CCHS-causing mutation do not respond to hypercapnia and die in the first hour after birth from central apnoea. They are therefore a reliable animal model for CCHS. Neurons of the retrotrapezoïd nucleus/parafacial respiratory group (RTN/pFRG) were found severely depleted in these mice and no other neuronal loss could be identified. Physiological experiments show that RTN/pFRG neurons are crucial to driving proper breathing at birth and are necessary for central chemoreception and the generation of a normal respiratory rhythm. To date, the reason for the selective vulnerability of RTN/pFRG neurons to PHOX2B protein dysfunction remains unexplained.

  • sleep disordered breathing in newborn mice heterozygous for the transcription factor phox2b
    American Journal of Respiratory and Critical Care Medicine, 2005
    Co-Authors: Estelle Durand, Christo Goridis, Stephane Dauger, Alexandre Pattyn, Claude Gaultier, Jorge Gallego
    Abstract:

    Rationale: Central Congenital Hypoventilation Syndrome (CCHS) is a rare autosomal dominant Syndrome present from birth, and characterized by depressed ventilation during sleep. Heterozygous mutations of the homeobox gene Phox2b were recently found in a very high proportion of patients. Objectives: To determine whether newborn mice with heterozygous targeted deletion of the transcription factor Phox2b would display sleep-disordered breathing. Methods: We measured breathing pattern using whole-body plethysmography in wild-type and mutant 5-day-old mice, and we classified sleep–wake states using nuchal EMG and behavioral scores. Results: We found that sleep apnea total time was approximately six times longer (8.9 ± 12 vs. 1.5 ± 2.2 seconds, p < 0.0015), and ventilation during active sleep was 21% lower (18.4 ± 5.1 vs. 23.3 ± 5.5 ml/g/second, p < 0.006) in mutant than in wild-type pups. During wakefulness, apnea time and ventilation were not significantly different between mutant and wild-type pups. Mutant an...

Toru Ishizuka - One of the best experts on this subject based on the ideXlab platform.

  • RESEARCH ARTICLE A Phox2b BAC Transgenic Rat Line Useful for Understanding Respiratory Rhythm Generator Neural Circuitry
    2016
    Co-Authors: Keiko Ikeda, Masanori Takahashi, Shigeru Sato, Hiroyuki Igarashi, Toru Ishizuka, Hiromu Yawo, Satoru Arata, Michelle E. Southard-smith, Kiyoshi Kawakami
    Abstract:

    The key role of the respiratory neural center is respiratory rhythm generation to maintain homeostasis through the control of arterial blood pCO2/pH and pO2 levels. The neuronal network responsible for respiratory rhythm generation in neonatal rat resides in the ventral side of the medulla and is composed of two groups; the parafacial respiratory group (pFRG) and the pre-Bötzinger complex group (preBötC). The pFRG partially overlaps in the retrotra-pezoid nucleus (RTN), which was originally identified in adult cats and rats. Part of the pre-inspiratory (Pre-I) neurons in the RTN/pFRG serves as central chemoreceptor neurons and the CO2 sensitive Pre-I neurons express homeobox gene Phox2b. Phox2b encodes a tran-scription factor and is essential for the development of the sensory-motor visceral circuits. Mutations in human PHOX2B cause Congenital Hypoventilation Syndrome, which is charac-terized by blunted ventilatory response to hypercapnia. Here we describe the generation of a novel transgenic (Tg) rat harboring fluorescently labeled Pre-I neurons in the RTN/pFRG. In addition, the Tg rat showed fluorescent signals in autonomic enteric neurons and carotid bodies. Because the Tg rat expresses inducible Cre recombinase in PHOX2B-positive cell

  • a phox2b bac transgenic rat line useful for understanding respiratory rhythm generator neural circuitry
    PLOS ONE, 2015
    Co-Authors: Keiko Ikeda, Masanori Takahashi, Shigeru Sato, Hiroyuki Igarashi, Toru Ishizuka, Hiromu Yawo, Satoru Arata, Michelle E Southardsmith, Kiyoshi Kawakami
    Abstract:

    The key role of the respiratory neural center is respiratory rhythm generation to maintain homeostasis through the control of arterial blood pCO2/pH and pO2 levels. The neuronal network responsible for respiratory rhythm generation in neonatal rat resides in the ventral side of the medulla and is composed of two groups; the parafacial respiratory group (pFRG) and the pre-Botzinger complex group (preBotC). The pFRG partially overlaps in the retrotrapezoid nucleus (RTN), which was originally identified in adult cats and rats. Part of the pre-inspiratory (Pre-I) neurons in the RTN/pFRG serves as central chemoreceptor neurons and the CO2 sensitive Pre-I neurons express homeobox gene Phox2b. Phox2b encodes a transcription factor and is essential for the development of the sensory-motor visceral circuits. Mutations in human PHOX2B cause Congenital Hypoventilation Syndrome, which is characterized by blunted ventilatory response to hypercapnia. Here we describe the generation of a novel transgenic (Tg) rat harboring fluorescently labeled Pre-I neurons in the RTN/pFRG. In addition, the Tg rat showed fluorescent signals in autonomic enteric neurons and carotid bodies. Because the Tg rat expresses inducible Cre recombinase in PHOX2B-positive cells during development, it is a potentially powerful tool for dissecting the entire picture of the respiratory neural network during development and for identifying the CO2/O2 sensor molecules in the adult central and peripheral nervous systems.

Masanori Takahashi - One of the best experts on this subject based on the ideXlab platform.

  • RESEARCH ARTICLE A Phox2b BAC Transgenic Rat Line Useful for Understanding Respiratory Rhythm Generator Neural Circuitry
    2016
    Co-Authors: Keiko Ikeda, Masanori Takahashi, Shigeru Sato, Hiroyuki Igarashi, Toru Ishizuka, Hiromu Yawo, Satoru Arata, Michelle E. Southard-smith, Kiyoshi Kawakami
    Abstract:

    The key role of the respiratory neural center is respiratory rhythm generation to maintain homeostasis through the control of arterial blood pCO2/pH and pO2 levels. The neuronal network responsible for respiratory rhythm generation in neonatal rat resides in the ventral side of the medulla and is composed of two groups; the parafacial respiratory group (pFRG) and the pre-Bötzinger complex group (preBötC). The pFRG partially overlaps in the retrotra-pezoid nucleus (RTN), which was originally identified in adult cats and rats. Part of the pre-inspiratory (Pre-I) neurons in the RTN/pFRG serves as central chemoreceptor neurons and the CO2 sensitive Pre-I neurons express homeobox gene Phox2b. Phox2b encodes a tran-scription factor and is essential for the development of the sensory-motor visceral circuits. Mutations in human PHOX2B cause Congenital Hypoventilation Syndrome, which is charac-terized by blunted ventilatory response to hypercapnia. Here we describe the generation of a novel transgenic (Tg) rat harboring fluorescently labeled Pre-I neurons in the RTN/pFRG. In addition, the Tg rat showed fluorescent signals in autonomic enteric neurons and carotid bodies. Because the Tg rat expresses inducible Cre recombinase in PHOX2B-positive cell

  • a phox2b bac transgenic rat line useful for understanding respiratory rhythm generator neural circuitry
    PLOS ONE, 2015
    Co-Authors: Keiko Ikeda, Masanori Takahashi, Shigeru Sato, Hiroyuki Igarashi, Toru Ishizuka, Hiromu Yawo, Satoru Arata, Michelle E Southardsmith, Kiyoshi Kawakami
    Abstract:

    The key role of the respiratory neural center is respiratory rhythm generation to maintain homeostasis through the control of arterial blood pCO2/pH and pO2 levels. The neuronal network responsible for respiratory rhythm generation in neonatal rat resides in the ventral side of the medulla and is composed of two groups; the parafacial respiratory group (pFRG) and the pre-Botzinger complex group (preBotC). The pFRG partially overlaps in the retrotrapezoid nucleus (RTN), which was originally identified in adult cats and rats. Part of the pre-inspiratory (Pre-I) neurons in the RTN/pFRG serves as central chemoreceptor neurons and the CO2 sensitive Pre-I neurons express homeobox gene Phox2b. Phox2b encodes a transcription factor and is essential for the development of the sensory-motor visceral circuits. Mutations in human PHOX2B cause Congenital Hypoventilation Syndrome, which is characterized by blunted ventilatory response to hypercapnia. Here we describe the generation of a novel transgenic (Tg) rat harboring fluorescently labeled Pre-I neurons in the RTN/pFRG. In addition, the Tg rat showed fluorescent signals in autonomic enteric neurons and carotid bodies. Because the Tg rat expresses inducible Cre recombinase in PHOX2B-positive cells during development, it is a potentially powerful tool for dissecting the entire picture of the respiratory neural network during development and for identifying the CO2/O2 sensor molecules in the adult central and peripheral nervous systems.