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Yoshinori Takagi - One of the best experts on this subject based on the ideXlab platform.
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Continuous 2-Keto-l-gulonic acid Fermentation by mixed culture of Ketogulonicigenium vulgare DSM 4025 and Bacillus megaterium or Xanthomonas maltophilia
Applied Microbiology and Biotechnology, 2010Co-Authors: Yoshinori Takagi, Teruhide Sugisawa, Tatsuo HoshinoAbstract:The Fermentation Process of 2-keto-L-gulonic acid (2KGA) from L-sorbose was developed using a two-stage Continuous Fermentation system. The mixed culture of Ketogulonicigenium vulgare DSM 4025 and Bacillus megaterium DSM 4026 produced 90 g/L of 2KGA from 120 g/L of L-sorbose at the dilution rate of 0.01 h^−1 in a single-stage Continuous Fermentation Process. But after the production period was beyond 150 h, the significant decrease of 2KGA productivity was observed. When the non-spore forming bacteria Xanthomonas maltophilia IFO 12692 was used instead of B . megaterium DSM 4026 as a partner strain for K . vulgare DSM 4025, the 2KGA productivity was significantly improved in a two-stage Continuous culture mode, in which two fermentors of the same size and volume were connected in series. In this mode, with two sets of 3-L jar fermentors, the steady state could be continued to over 1,331.5 h at least, when the dilution rates were 0.0382 h^−1 and 0.0380 hour^−1, respectively, for the first and second fermentors. The overall productivity was calculated to be 2.15 g/L/h at 113.1 g/L and a molar conversion yield of 90.1%. In the up-scaling Fermentation to 30-L jar fermentors, 118.5 g/L of 2KGA was produced when dilution rates in both stages were 0.0430 hour^−1, and the overall productivity was calculated to be 2.55 g/L/h.
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Continuous 2 keto l gulonic acid Fermentation from l sorbose by ketogulonigenium vulgare dsm 4025
Applied Microbiology and Biotechnology, 2009Co-Authors: Yoshinori Takagi, Teruhide Sugisawa, Tatsuo Kanagawaken HoshinoAbstract:A single-stage Continuous Fermentation Process for the production of 2-keto-l-gulonic acid (2KGA) from l-sorbose using Ketogulonigenium vulgare DSM 4025 was developed. The chemostat culture with the dilution rate that was calculated based on the relationship between the 2KGA production rate and the 2KGA concentration was feasible for production with high concentration of 2KGA. In this system, 112.2 g/L of 2KGA on the average was Continuously produced from 114 g/L of l-sorbose. A steady state of the Fermentation was maintained for the duration of more than 110 h. The dilution rate was kept in the range of 0.035 and 0.043 h−1, and the 2KGA productivity was 3.90 to 4.80 g/L/h. The average molar conversion yield of 2KGA from l-sorbose was 91.3%. Under the optimal conditions, l-sorbose concentration was kept at 0 g/L. Meanwhile, the dissolved oxygen level was changing in response to the dilution rate and 2KGA concentration. In the dissolved oxygen (DO) range of 16% to 58%, it was revealed that the relationship between DO and D possessed high degree of positive correlation under the l-sorbose limiting condition (complete consumption of l-sorbose). Increasing D closer to the critical value for washing out point of the Continuous Fermentation, DO value tended to be gradually increased up to 58%. In conclusion, an efficient and reproducible Continuous Fermentation Process for 2KGA production by K. vulgare DSM 4025 could be developed using a medium containing baker’s yeast without using a second helper microorganism.
Teruhide Sugisawa - One of the best experts on this subject based on the ideXlab platform.
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Continuous 2-Keto-l-gulonic acid Fermentation by mixed culture of Ketogulonicigenium vulgare DSM 4025 and Bacillus megaterium or Xanthomonas maltophilia
Applied Microbiology and Biotechnology, 2010Co-Authors: Yoshinori Takagi, Teruhide Sugisawa, Tatsuo HoshinoAbstract:The Fermentation Process of 2-keto-L-gulonic acid (2KGA) from L-sorbose was developed using a two-stage Continuous Fermentation system. The mixed culture of Ketogulonicigenium vulgare DSM 4025 and Bacillus megaterium DSM 4026 produced 90 g/L of 2KGA from 120 g/L of L-sorbose at the dilution rate of 0.01 h^−1 in a single-stage Continuous Fermentation Process. But after the production period was beyond 150 h, the significant decrease of 2KGA productivity was observed. When the non-spore forming bacteria Xanthomonas maltophilia IFO 12692 was used instead of B . megaterium DSM 4026 as a partner strain for K . vulgare DSM 4025, the 2KGA productivity was significantly improved in a two-stage Continuous culture mode, in which two fermentors of the same size and volume were connected in series. In this mode, with two sets of 3-L jar fermentors, the steady state could be continued to over 1,331.5 h at least, when the dilution rates were 0.0382 h^−1 and 0.0380 hour^−1, respectively, for the first and second fermentors. The overall productivity was calculated to be 2.15 g/L/h at 113.1 g/L and a molar conversion yield of 90.1%. In the up-scaling Fermentation to 30-L jar fermentors, 118.5 g/L of 2KGA was produced when dilution rates in both stages were 0.0430 hour^−1, and the overall productivity was calculated to be 2.55 g/L/h.
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Continuous 2 keto l gulonic acid Fermentation from l sorbose by ketogulonigenium vulgare dsm 4025
Applied Microbiology and Biotechnology, 2009Co-Authors: Yoshinori Takagi, Teruhide Sugisawa, Tatsuo Kanagawaken HoshinoAbstract:A single-stage Continuous Fermentation Process for the production of 2-keto-l-gulonic acid (2KGA) from l-sorbose using Ketogulonigenium vulgare DSM 4025 was developed. The chemostat culture with the dilution rate that was calculated based on the relationship between the 2KGA production rate and the 2KGA concentration was feasible for production with high concentration of 2KGA. In this system, 112.2 g/L of 2KGA on the average was Continuously produced from 114 g/L of l-sorbose. A steady state of the Fermentation was maintained for the duration of more than 110 h. The dilution rate was kept in the range of 0.035 and 0.043 h−1, and the 2KGA productivity was 3.90 to 4.80 g/L/h. The average molar conversion yield of 2KGA from l-sorbose was 91.3%. Under the optimal conditions, l-sorbose concentration was kept at 0 g/L. Meanwhile, the dissolved oxygen level was changing in response to the dilution rate and 2KGA concentration. In the dissolved oxygen (DO) range of 16% to 58%, it was revealed that the relationship between DO and D possessed high degree of positive correlation under the l-sorbose limiting condition (complete consumption of l-sorbose). Increasing D closer to the critical value for washing out point of the Continuous Fermentation, DO value tended to be gradually increased up to 58%. In conclusion, an efficient and reproducible Continuous Fermentation Process for 2KGA production by K. vulgare DSM 4025 could be developed using a medium containing baker’s yeast without using a second helper microorganism.
Tatsuo Hoshino - One of the best experts on this subject based on the ideXlab platform.
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Continuous 2-Keto-l-gulonic acid Fermentation by mixed culture of Ketogulonicigenium vulgare DSM 4025 and Bacillus megaterium or Xanthomonas maltophilia
Applied Microbiology and Biotechnology, 2010Co-Authors: Yoshinori Takagi, Teruhide Sugisawa, Tatsuo HoshinoAbstract:The Fermentation Process of 2-keto-L-gulonic acid (2KGA) from L-sorbose was developed using a two-stage Continuous Fermentation system. The mixed culture of Ketogulonicigenium vulgare DSM 4025 and Bacillus megaterium DSM 4026 produced 90 g/L of 2KGA from 120 g/L of L-sorbose at the dilution rate of 0.01 h^−1 in a single-stage Continuous Fermentation Process. But after the production period was beyond 150 h, the significant decrease of 2KGA productivity was observed. When the non-spore forming bacteria Xanthomonas maltophilia IFO 12692 was used instead of B . megaterium DSM 4026 as a partner strain for K . vulgare DSM 4025, the 2KGA productivity was significantly improved in a two-stage Continuous culture mode, in which two fermentors of the same size and volume were connected in series. In this mode, with two sets of 3-L jar fermentors, the steady state could be continued to over 1,331.5 h at least, when the dilution rates were 0.0382 h^−1 and 0.0380 hour^−1, respectively, for the first and second fermentors. The overall productivity was calculated to be 2.15 g/L/h at 113.1 g/L and a molar conversion yield of 90.1%. In the up-scaling Fermentation to 30-L jar fermentors, 118.5 g/L of 2KGA was produced when dilution rates in both stages were 0.0430 hour^−1, and the overall productivity was calculated to be 2.55 g/L/h.
Tatsuo Kanagawaken Hoshino - One of the best experts on this subject based on the ideXlab platform.
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Continuous 2 keto l gulonic acid Fermentation from l sorbose by ketogulonigenium vulgare dsm 4025
Applied Microbiology and Biotechnology, 2009Co-Authors: Yoshinori Takagi, Teruhide Sugisawa, Tatsuo Kanagawaken HoshinoAbstract:A single-stage Continuous Fermentation Process for the production of 2-keto-l-gulonic acid (2KGA) from l-sorbose using Ketogulonigenium vulgare DSM 4025 was developed. The chemostat culture with the dilution rate that was calculated based on the relationship between the 2KGA production rate and the 2KGA concentration was feasible for production with high concentration of 2KGA. In this system, 112.2 g/L of 2KGA on the average was Continuously produced from 114 g/L of l-sorbose. A steady state of the Fermentation was maintained for the duration of more than 110 h. The dilution rate was kept in the range of 0.035 and 0.043 h−1, and the 2KGA productivity was 3.90 to 4.80 g/L/h. The average molar conversion yield of 2KGA from l-sorbose was 91.3%. Under the optimal conditions, l-sorbose concentration was kept at 0 g/L. Meanwhile, the dissolved oxygen level was changing in response to the dilution rate and 2KGA concentration. In the dissolved oxygen (DO) range of 16% to 58%, it was revealed that the relationship between DO and D possessed high degree of positive correlation under the l-sorbose limiting condition (complete consumption of l-sorbose). Increasing D closer to the critical value for washing out point of the Continuous Fermentation, DO value tended to be gradually increased up to 58%. In conclusion, an efficient and reproducible Continuous Fermentation Process for 2KGA production by K. vulgare DSM 4025 could be developed using a medium containing baker’s yeast without using a second helper microorganism.
Philippe Soucaille - One of the best experts on this subject based on the ideXlab platform.
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a new Process for the Continuous production of succinic acid from glucose at high yield titer and productivity
Biotechnology and Bioengineering, 2008Co-Authors: Isabelle Meynialsalles, Sophie Dorotyn, Philippe SoucailleAbstract:A novel three stages Continuous Fermentation Process for the bioproduction of succinic acid at high concentration, productivity and yield using A. succiniciproducens was developed. This Process combined an integrated membrane-bioreactor-electrodialysis system. An energetic characterization of A. succiniciproducens during anaerobic cultured in a cell recycle bioreactor was done first. The very low value of YATP obtained suggests that an ATP dependent mechanism of succinate export is present in A. succiniciproducens. Under the best culture conditions, biomass concentration and succinate volumetric productivity reach values of 42 g/L and 14.8 g/L·h. These values are respectively 28 and 20 times higher compared to batch cultures done in our laboratory. To limit end-products inhibition on growth, a mono-polar electrodialysis pilot was secondly coupled to the cell recycle bioreactor. This system allowed to Continuously remove succinate and acetate from the permeate and recycle an organic acids depleted solution in the reactor. The integrated membrane-bioreactor-electrodialysis Process produced a five times concentrated succinate solution (83 g/L) compared to the cell recycle reactor system, at a high average succinate yield of 1.35 mol/mol and a slightly lower volumetric productivity of 10.4 g/L·h. The Process combined maximal production yield to high productivity and titer and could be economically viable for the development of a biological route for succinic acid production. Biotechnol. Bioeng. 2008;99: 129–135. © 2007 Wiley Periodicals, Inc.