The Experts below are selected from a list of 4230 Experts worldwide ranked by ideXlab platform
Denitsa Docheva - One of the best experts on this subject based on the ideXlab platform.
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Functionalized thermosensitive hydrogel combined with tendon stem/progenitor cells as injectable cell delivery carrier for tendon tissue engineering.
Biomedical materials (Bristol England), 2018Co-Authors: Heyong Yin, Zexing Yan, Richard J Bauer, Jiang Peng, Matthias Schieker, Michael Nerlich, Denitsa DochevaAbstract:Thermosensitive hydrogels have been studied for potential application as promising alternative cell carriers in cell-based regenerative therapies. In this study, a thermosensitive butane diisocyanate (BDI)-collagen hydrogel (BC hydrogel) was designed as an injectable cell delivery carrier of tendon stem/progenitor cells (TSPCs) for tendon tissue engineering. We functionalized the BDI hydrogel with the addition of 20% (v/v) collagen I gel to obtain the thermosensitive BC hydrogel, which was then seeded with TSPCs derived from human Achilles tendons. The BC hydrogel compatibility and TSPC behavior and molecular response to the 3D hydrogel were investigated. Collagen (COL) I gel served as a control group. Our findings demonstrated that the BC hydrogel was thermosensitive, and hardened above 25 °C. It supported TSPC survival, proliferation, and metabolic activity with satisfactory dimension stability and biocompatibility, as revealed by gel Contraction Assay, live/dead staining, DNA quantification, and resazurin metabolic Assay. Phalloidin-based visualization of F-actin demonstrated that the TSPCs were stretched within COL I gel with classical spindle cell shapes; similar cell morphologies were also found in the BC hydrogel. The gene expression profile of TSPCs in the BC hydrogel was comparable with that in COL I gel. Moreover, the BC hydrogel supported capillary-like structure formation by human umbilical vein endothelial cells (HUVECs) in the hydrogel matrix. Taken together, these results suggest that the thermosensitive BC hydrogel holds great potential as an injectable cell delivery carrier of TSPCs for tendon tissue engineering.
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functionalized thermosensitive hydrogel combined with tendon stem progenitor cells as injectable cell delivery carrier for tendon tissue engineering
Biomedical Materials, 2018Co-Authors: Heyong Yin, Zexing Yan, Richard J Bauer, Jiang Peng, Matthias Schieker, Michael Nerlich, Denitsa DochevaAbstract:Thermosensitive hydrogels have been studied for potential application as promising alternative cell carriers in cell-based regenerative therapies. In this study, a thermosensitive butane diisocyanate (BDI)-collagen hydrogel (BC hydrogel) was designed as an injectable cell delivery carrier of tendon stem/progenitor cells (TSPCs) for tendon tissue engineering. We functionalized the BDI hydrogel with the addition of 20% (v/v) collagen I gel to obtain the thermosensitive BC hydrogel, which was then seeded with TSPCs derived from human Achilles tendons. The BC hydrogel compatibility and TSPC behavior and molecular response to the 3D hydrogel were investigated. Collagen (COL) I gel served as a control group. Our findings demonstrated that the BC hydrogel was thermosensitive, and hardened above 25 °C. It supported TSPC survival, proliferation, and metabolic activity with satisfactory dimension stability and biocompatibility, as revealed by gel Contraction Assay, live/dead staining, DNA quantification, and resazurin metabolic Assay. Phalloidin-based visualization of F-actin demonstrated that the TSPCs were stretched within COL I gel with classical spindle cell shapes; similar cell morphologies were also found in the BC hydrogel. The gene expression profile of TSPCs in the BC hydrogel was comparable with that in COL I gel. Moreover, the BC hydrogel supported capillary-like structure formation by human umbilical vein endothelial cells (HUVECs) in the hydrogel matrix. Taken together, these results suggest that the thermosensitive BC hydrogel holds great potential as an injectable cell delivery carrier of TSPCs for tendon tissue engineering.
Matthias Schieker - One of the best experts on this subject based on the ideXlab platform.
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Functionalized thermosensitive hydrogel combined with tendon stem/progenitor cells as injectable cell delivery carrier for tendon tissue engineering.
Biomedical materials (Bristol England), 2018Co-Authors: Heyong Yin, Zexing Yan, Richard J Bauer, Jiang Peng, Matthias Schieker, Michael Nerlich, Denitsa DochevaAbstract:Thermosensitive hydrogels have been studied for potential application as promising alternative cell carriers in cell-based regenerative therapies. In this study, a thermosensitive butane diisocyanate (BDI)-collagen hydrogel (BC hydrogel) was designed as an injectable cell delivery carrier of tendon stem/progenitor cells (TSPCs) for tendon tissue engineering. We functionalized the BDI hydrogel with the addition of 20% (v/v) collagen I gel to obtain the thermosensitive BC hydrogel, which was then seeded with TSPCs derived from human Achilles tendons. The BC hydrogel compatibility and TSPC behavior and molecular response to the 3D hydrogel were investigated. Collagen (COL) I gel served as a control group. Our findings demonstrated that the BC hydrogel was thermosensitive, and hardened above 25 °C. It supported TSPC survival, proliferation, and metabolic activity with satisfactory dimension stability and biocompatibility, as revealed by gel Contraction Assay, live/dead staining, DNA quantification, and resazurin metabolic Assay. Phalloidin-based visualization of F-actin demonstrated that the TSPCs were stretched within COL I gel with classical spindle cell shapes; similar cell morphologies were also found in the BC hydrogel. The gene expression profile of TSPCs in the BC hydrogel was comparable with that in COL I gel. Moreover, the BC hydrogel supported capillary-like structure formation by human umbilical vein endothelial cells (HUVECs) in the hydrogel matrix. Taken together, these results suggest that the thermosensitive BC hydrogel holds great potential as an injectable cell delivery carrier of TSPCs for tendon tissue engineering.
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functionalized thermosensitive hydrogel combined with tendon stem progenitor cells as injectable cell delivery carrier for tendon tissue engineering
Biomedical Materials, 2018Co-Authors: Heyong Yin, Zexing Yan, Richard J Bauer, Jiang Peng, Matthias Schieker, Michael Nerlich, Denitsa DochevaAbstract:Thermosensitive hydrogels have been studied for potential application as promising alternative cell carriers in cell-based regenerative therapies. In this study, a thermosensitive butane diisocyanate (BDI)-collagen hydrogel (BC hydrogel) was designed as an injectable cell delivery carrier of tendon stem/progenitor cells (TSPCs) for tendon tissue engineering. We functionalized the BDI hydrogel with the addition of 20% (v/v) collagen I gel to obtain the thermosensitive BC hydrogel, which was then seeded with TSPCs derived from human Achilles tendons. The BC hydrogel compatibility and TSPC behavior and molecular response to the 3D hydrogel were investigated. Collagen (COL) I gel served as a control group. Our findings demonstrated that the BC hydrogel was thermosensitive, and hardened above 25 °C. It supported TSPC survival, proliferation, and metabolic activity with satisfactory dimension stability and biocompatibility, as revealed by gel Contraction Assay, live/dead staining, DNA quantification, and resazurin metabolic Assay. Phalloidin-based visualization of F-actin demonstrated that the TSPCs were stretched within COL I gel with classical spindle cell shapes; similar cell morphologies were also found in the BC hydrogel. The gene expression profile of TSPCs in the BC hydrogel was comparable with that in COL I gel. Moreover, the BC hydrogel supported capillary-like structure formation by human umbilical vein endothelial cells (HUVECs) in the hydrogel matrix. Taken together, these results suggest that the thermosensitive BC hydrogel holds great potential as an injectable cell delivery carrier of TSPCs for tendon tissue engineering.
Heyong Yin - One of the best experts on this subject based on the ideXlab platform.
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Functionalized thermosensitive hydrogel combined with tendon stem/progenitor cells as injectable cell delivery carrier for tendon tissue engineering.
Biomedical materials (Bristol England), 2018Co-Authors: Heyong Yin, Zexing Yan, Richard J Bauer, Jiang Peng, Matthias Schieker, Michael Nerlich, Denitsa DochevaAbstract:Thermosensitive hydrogels have been studied for potential application as promising alternative cell carriers in cell-based regenerative therapies. In this study, a thermosensitive butane diisocyanate (BDI)-collagen hydrogel (BC hydrogel) was designed as an injectable cell delivery carrier of tendon stem/progenitor cells (TSPCs) for tendon tissue engineering. We functionalized the BDI hydrogel with the addition of 20% (v/v) collagen I gel to obtain the thermosensitive BC hydrogel, which was then seeded with TSPCs derived from human Achilles tendons. The BC hydrogel compatibility and TSPC behavior and molecular response to the 3D hydrogel were investigated. Collagen (COL) I gel served as a control group. Our findings demonstrated that the BC hydrogel was thermosensitive, and hardened above 25 °C. It supported TSPC survival, proliferation, and metabolic activity with satisfactory dimension stability and biocompatibility, as revealed by gel Contraction Assay, live/dead staining, DNA quantification, and resazurin metabolic Assay. Phalloidin-based visualization of F-actin demonstrated that the TSPCs were stretched within COL I gel with classical spindle cell shapes; similar cell morphologies were also found in the BC hydrogel. The gene expression profile of TSPCs in the BC hydrogel was comparable with that in COL I gel. Moreover, the BC hydrogel supported capillary-like structure formation by human umbilical vein endothelial cells (HUVECs) in the hydrogel matrix. Taken together, these results suggest that the thermosensitive BC hydrogel holds great potential as an injectable cell delivery carrier of TSPCs for tendon tissue engineering.
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functionalized thermosensitive hydrogel combined with tendon stem progenitor cells as injectable cell delivery carrier for tendon tissue engineering
Biomedical Materials, 2018Co-Authors: Heyong Yin, Zexing Yan, Richard J Bauer, Jiang Peng, Matthias Schieker, Michael Nerlich, Denitsa DochevaAbstract:Thermosensitive hydrogels have been studied for potential application as promising alternative cell carriers in cell-based regenerative therapies. In this study, a thermosensitive butane diisocyanate (BDI)-collagen hydrogel (BC hydrogel) was designed as an injectable cell delivery carrier of tendon stem/progenitor cells (TSPCs) for tendon tissue engineering. We functionalized the BDI hydrogel with the addition of 20% (v/v) collagen I gel to obtain the thermosensitive BC hydrogel, which was then seeded with TSPCs derived from human Achilles tendons. The BC hydrogel compatibility and TSPC behavior and molecular response to the 3D hydrogel were investigated. Collagen (COL) I gel served as a control group. Our findings demonstrated that the BC hydrogel was thermosensitive, and hardened above 25 °C. It supported TSPC survival, proliferation, and metabolic activity with satisfactory dimension stability and biocompatibility, as revealed by gel Contraction Assay, live/dead staining, DNA quantification, and resazurin metabolic Assay. Phalloidin-based visualization of F-actin demonstrated that the TSPCs were stretched within COL I gel with classical spindle cell shapes; similar cell morphologies were also found in the BC hydrogel. The gene expression profile of TSPCs in the BC hydrogel was comparable with that in COL I gel. Moreover, the BC hydrogel supported capillary-like structure formation by human umbilical vein endothelial cells (HUVECs) in the hydrogel matrix. Taken together, these results suggest that the thermosensitive BC hydrogel holds great potential as an injectable cell delivery carrier of TSPCs for tendon tissue engineering.
Akio Mori - One of the best experts on this subject based on the ideXlab platform.
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A Contraction Assay system using primary cultured mouse bronchial smooth muscle cells.
International archives of allergy and immunology, 2013Co-Authors: Satoshi Kouyama, Noriko Kitamura, Osamu Kaminuma, Akemi Otomo-abe, Akio MoriAbstract:Contraction of bronchial smooth muscle is the main mechanism of asthmatic responses. Inflammatory cells such as eosinophils and mast cells produce chemical mediators that induce smooth muscle Contraction. To investigate the mechanisms of IgE-independent asthmatic response in murine asthma models, a novel in vitro Assay system using primary cultured mouse bronchial smooth muscle cells (BSMC) was explored. Trachea and bronchi were taken from young mice and underwent primary culture. BSMC were expanded in culture and then embedded in a collagen gel. The well-known constrictors leukotriene D4 (LTD4), methacholine and histamine were applied to the BSMC gels. The gel images were captured by an image analyzer and contractile responses were evaluated. LTD4 and methacholine significantly induced the gel Contraction in a dose-dependent manner, but histamine did not. Montelukast, a CysLT type ΙΙ receptor antagonist, and atropine, a muscarinic acetylcholine receptor antagonist, inhibited the contractile responses in an agonist-specific manner. A Contraction Assay system using cultured mouse BSMC was successfully established for the first time. It may go a long way toward identifying bronchoconstricting mediators involved in murine asthma models. Copyright © 2013 S. Karger AG, Basel.
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Evaluation of cysteinyl leukotriene-induced Contraction of human cultured bronchial smooth muscle cells.
International archives of allergy and immunology, 2009Co-Authors: Noriko Kitamura, Osamu Kaminuma, Noriaki Kobayashi, Takayuki Ohtomo, Nobutaka Kiyokawa, Matsunobu Suko, Akio MoriAbstract:Cysteinyl leukotrienes (cysLTs) are major mediators involved in bronchial asthma, particularly bronchial constriction. However, a contractile response of human bronchial smooth muscle cells (hBSMCs) to cysLTs has not been well characterized at cellular level. A Contraction Assay using collagen gel embedded with cultured hBSMCs was established to analyze contractile responses at cellular level. Contractile responses to several constrictors including cysLTs were evaluated in 6-day cultured gels with varying fetal bovine serum (FBS) concentrations. Removal of FBS from the culture for the designated time periods resulted in increased contractile responses. CysLT-induced Contraction of the gel became more pronounced and reproducible. CysLT(1)R expression on the hBSMCs was significantly increased by the removal of FBS. Contractile responses of hBSMCs to cysLTs can be evaluated at cellular level for the first time. This experimental system may be useful for the in vitro evaluation and future development of cysLTs antagonists. Copyright 2009 S. Karger AG, Basel.
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A Contraction Assay system using established human bronchial smooth muscle cells.
International Archives of Allergy and Immunology, 2008Co-Authors: Noriko Kitamura, Osamu Kaminuma, Noriaki Kobayashi, Akio MoriAbstract:Background: To further understand the mechanisms of airway obstruction in asthma, it is crucial to investigate contractile responses of human airway smooth muscle. An in vitro Assay system employing collagen gels embedded with well-established bronchial smooth muscle cells of human origin was explored in the present study. Methods: Commercially available cultured human bronchial smooth muscle cells were embedded into a collagen gel. Well-known constrictors, histamine and methacholine, were added to the gel. The gel images were captured by an image analyzer, and contractile responses were evaluated. Results: Histamine and methacholine induced Contraction of the gels in a dose-dependent manner. Pyrilamine, an H1 receptor antagonist, inhibited gel Contraction in an agonist-specific manner. Conclusion: Our Contraction Assay system, employing widely distributed cultured cells, was highly reproducible and precise. It may go a long way toward understanding mechanisms of asthmatic responses and evaluation of antiasthma drugs.
Michael Nerlich - One of the best experts on this subject based on the ideXlab platform.
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Functionalized thermosensitive hydrogel combined with tendon stem/progenitor cells as injectable cell delivery carrier for tendon tissue engineering.
Biomedical materials (Bristol England), 2018Co-Authors: Heyong Yin, Zexing Yan, Richard J Bauer, Jiang Peng, Matthias Schieker, Michael Nerlich, Denitsa DochevaAbstract:Thermosensitive hydrogels have been studied for potential application as promising alternative cell carriers in cell-based regenerative therapies. In this study, a thermosensitive butane diisocyanate (BDI)-collagen hydrogel (BC hydrogel) was designed as an injectable cell delivery carrier of tendon stem/progenitor cells (TSPCs) for tendon tissue engineering. We functionalized the BDI hydrogel with the addition of 20% (v/v) collagen I gel to obtain the thermosensitive BC hydrogel, which was then seeded with TSPCs derived from human Achilles tendons. The BC hydrogel compatibility and TSPC behavior and molecular response to the 3D hydrogel were investigated. Collagen (COL) I gel served as a control group. Our findings demonstrated that the BC hydrogel was thermosensitive, and hardened above 25 °C. It supported TSPC survival, proliferation, and metabolic activity with satisfactory dimension stability and biocompatibility, as revealed by gel Contraction Assay, live/dead staining, DNA quantification, and resazurin metabolic Assay. Phalloidin-based visualization of F-actin demonstrated that the TSPCs were stretched within COL I gel with classical spindle cell shapes; similar cell morphologies were also found in the BC hydrogel. The gene expression profile of TSPCs in the BC hydrogel was comparable with that in COL I gel. Moreover, the BC hydrogel supported capillary-like structure formation by human umbilical vein endothelial cells (HUVECs) in the hydrogel matrix. Taken together, these results suggest that the thermosensitive BC hydrogel holds great potential as an injectable cell delivery carrier of TSPCs for tendon tissue engineering.
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functionalized thermosensitive hydrogel combined with tendon stem progenitor cells as injectable cell delivery carrier for tendon tissue engineering
Biomedical Materials, 2018Co-Authors: Heyong Yin, Zexing Yan, Richard J Bauer, Jiang Peng, Matthias Schieker, Michael Nerlich, Denitsa DochevaAbstract:Thermosensitive hydrogels have been studied for potential application as promising alternative cell carriers in cell-based regenerative therapies. In this study, a thermosensitive butane diisocyanate (BDI)-collagen hydrogel (BC hydrogel) was designed as an injectable cell delivery carrier of tendon stem/progenitor cells (TSPCs) for tendon tissue engineering. We functionalized the BDI hydrogel with the addition of 20% (v/v) collagen I gel to obtain the thermosensitive BC hydrogel, which was then seeded with TSPCs derived from human Achilles tendons. The BC hydrogel compatibility and TSPC behavior and molecular response to the 3D hydrogel were investigated. Collagen (COL) I gel served as a control group. Our findings demonstrated that the BC hydrogel was thermosensitive, and hardened above 25 °C. It supported TSPC survival, proliferation, and metabolic activity with satisfactory dimension stability and biocompatibility, as revealed by gel Contraction Assay, live/dead staining, DNA quantification, and resazurin metabolic Assay. Phalloidin-based visualization of F-actin demonstrated that the TSPCs were stretched within COL I gel with classical spindle cell shapes; similar cell morphologies were also found in the BC hydrogel. The gene expression profile of TSPCs in the BC hydrogel was comparable with that in COL I gel. Moreover, the BC hydrogel supported capillary-like structure formation by human umbilical vein endothelial cells (HUVECs) in the hydrogel matrix. Taken together, these results suggest that the thermosensitive BC hydrogel holds great potential as an injectable cell delivery carrier of TSPCs for tendon tissue engineering.