The Experts below are selected from a list of 293781 Experts worldwide ranked by ideXlab platform
Lori Sussel - One of the best experts on this subject based on the ideXlab platform.
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islet β cell specific mafa transcription requires the 5 flanking conserved region 3 Control Domain
Molecular and Cellular Biology, 2010Co-Authors: Jeffrey C Raum, Chad S Hunter, Isabella Artner, Eva Henderson, Lynda Elghazi, Beatriz Sosapineda, Takeshi Ogihara, Raghavendra G Mirmira, Lori SusselAbstract:MafA is a key transcriptional activator of islet beta cells and its exclusive expression within beta cells of the developing and adult pancreas is distinct amongst pancreatic regulators. Region 3 (base pairs -8118/-7750 relative to the transcription start site), one of six conserved 5' cis-Domains of the MafA promoter, is capable of directing beta-cell-line-selective expression. Transgenic reporters of Region 3 alone (R3), sequences spanning Regions 1-6 (R1-6; base pairs -10428/+230), and R1-6 lacking Region 3 (R1-6(DeltaR3)) were generated. Only the R1-6 transgene was active in MafA(+) insulin(+) cells during development and in adults. R1-6 also mediated glucose-induced MafA expression. Conversely, pancreatic expression was not observed with the R3 or R1-6(DeltaR3) lines, although much of the non-pancreatic expression pattern was shared between the R1-6 and R1-6(DeltaR3) lines. Further support for the importance of Region 3 was shown as the islet regulators Nkx6.1 and Pax6, but not NeuroD1 activated MafA using gel shift, chromatin immunoprecipitation (ChIP), transfection assays, and in vivo mouse knockout models. Lastly ChIP demonstrated that Pax6 and Pdx-1 bound also to Regions 1 and 6, potentially functioning in pancreatic and non-pancreatic expression. These data highlight the nature of the cis- and trans-acting factors Controlling the beta-3cell-specific expression of MafA. (Less)
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islet β cell specific mafa transcription requires the 5 flanking conserved region 3 Control Domain
Molecular and Cellular Biology, 2010Co-Authors: Jeffrey C Raum, Chad S Hunter, Isabella Artner, Eva Henderson, Lynda Elghazi, Beatriz Sosapineda, Takeshi Ogihara, Raghavendra G Mirmira, Min Guo, Lori SusselAbstract:MafA is a key transcriptional activator of islet beta cells, and its exclusive expression within beta cells of the developing and adult pancreas is distinct among pancreatic regulators. Region 3 (base pairs -8118 to -7750 relative to the transcription start site), one of six conserved 5' cis Domains of the MafA promoter, is capable of directing beta-cell-line-selective expression. Transgenic reporters of region 3 alone (R3), sequences spanning regions 1 to 6 (R1-6; base pairs -10428 to +230), and R1-6 lacking R3 (R1-6(DeltaR3)) were generated. Only the R1-6 transgene was active in MafA(+) insulin(+) cells during development and in adult cells. R1-6 also mediated glucose-induced MafA expression. Conversely, pancreatic expression was not observed with the R3 or R1-6(DeltaR3) line, although much of the nonpancreatic expression pattern was shared between the R1-6 and R1-6(DeltaR3) lines. Further support for the importance of R3 was also shown, as the islet regulators Nkx6.1 and Pax6, but not NeuroD1, activated MafA in gel shift, chromatin immunoprecipitation (ChIP), and transfection assays and in vivo mouse knockout models. Lastly, ChIP demonstrated that Pax6 and Pdx-1 also bound to R1 and R6, potentially functioning in pancreatic and nonpancreatic expression. These data highlight the nature of the cis- and trans-acting factors Controlling the beta-cell-specific expression of MafA.
Jeffrey C Raum - One of the best experts on this subject based on the ideXlab platform.
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islet β cell specific mafa transcription requires the 5 flanking conserved region 3 Control Domain
Molecular and Cellular Biology, 2010Co-Authors: Jeffrey C Raum, Chad S Hunter, Isabella Artner, Eva Henderson, Lynda Elghazi, Beatriz Sosapineda, Takeshi Ogihara, Raghavendra G Mirmira, Lori SusselAbstract:MafA is a key transcriptional activator of islet beta cells and its exclusive expression within beta cells of the developing and adult pancreas is distinct amongst pancreatic regulators. Region 3 (base pairs -8118/-7750 relative to the transcription start site), one of six conserved 5' cis-Domains of the MafA promoter, is capable of directing beta-cell-line-selective expression. Transgenic reporters of Region 3 alone (R3), sequences spanning Regions 1-6 (R1-6; base pairs -10428/+230), and R1-6 lacking Region 3 (R1-6(DeltaR3)) were generated. Only the R1-6 transgene was active in MafA(+) insulin(+) cells during development and in adults. R1-6 also mediated glucose-induced MafA expression. Conversely, pancreatic expression was not observed with the R3 or R1-6(DeltaR3) lines, although much of the non-pancreatic expression pattern was shared between the R1-6 and R1-6(DeltaR3) lines. Further support for the importance of Region 3 was shown as the islet regulators Nkx6.1 and Pax6, but not NeuroD1 activated MafA using gel shift, chromatin immunoprecipitation (ChIP), transfection assays, and in vivo mouse knockout models. Lastly ChIP demonstrated that Pax6 and Pdx-1 bound also to Regions 1 and 6, potentially functioning in pancreatic and non-pancreatic expression. These data highlight the nature of the cis- and trans-acting factors Controlling the beta-3cell-specific expression of MafA. (Less)
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islet β cell specific mafa transcription requires the 5 flanking conserved region 3 Control Domain
Molecular and Cellular Biology, 2010Co-Authors: Jeffrey C Raum, Chad S Hunter, Isabella Artner, Eva Henderson, Lynda Elghazi, Beatriz Sosapineda, Takeshi Ogihara, Raghavendra G Mirmira, Min Guo, Lori SusselAbstract:MafA is a key transcriptional activator of islet beta cells, and its exclusive expression within beta cells of the developing and adult pancreas is distinct among pancreatic regulators. Region 3 (base pairs -8118 to -7750 relative to the transcription start site), one of six conserved 5' cis Domains of the MafA promoter, is capable of directing beta-cell-line-selective expression. Transgenic reporters of region 3 alone (R3), sequences spanning regions 1 to 6 (R1-6; base pairs -10428 to +230), and R1-6 lacking R3 (R1-6(DeltaR3)) were generated. Only the R1-6 transgene was active in MafA(+) insulin(+) cells during development and in adult cells. R1-6 also mediated glucose-induced MafA expression. Conversely, pancreatic expression was not observed with the R3 or R1-6(DeltaR3) line, although much of the nonpancreatic expression pattern was shared between the R1-6 and R1-6(DeltaR3) lines. Further support for the importance of R3 was also shown, as the islet regulators Nkx6.1 and Pax6, but not NeuroD1, activated MafA in gel shift, chromatin immunoprecipitation (ChIP), and transfection assays and in vivo mouse knockout models. Lastly, ChIP demonstrated that Pax6 and Pdx-1 also bound to R1 and R6, potentially functioning in pancreatic and nonpancreatic expression. These data highlight the nature of the cis- and trans-acting factors Controlling the beta-cell-specific expression of MafA.
Maoning Tang - One of the best experts on this subject based on the ideXlab platform.
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optimal Control with state constraints for stochastic evolution equation with jumps in hilbert space
arXiv: Probability, 2017Co-Authors: Qingxin Meng, Maoning TangAbstract:This paper studies a stochastic optimal Control problem with state constraint, where the state equation is described by a Controlled stochastic evolution equation with jumps in Hilbert Space and the Control Domain is assumed to be convex. By means of Ekland variational principle, combining the convex variation method and the duality technique, necessary conditions for optimality are derived in the form of stochastic maximum principles.
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stochastic maximum principle of near optimal Control of fully coupled forward backward stochastic differential equation
Abstract and Applied Analysis, 2014Co-Authors: Maoning TangAbstract:This paper first makes an attempt to investigate the near-optimal Control of systems governed by fully nonlinear coupled forward-backward stochastic differential equations (FBSDEs) under the assumption of a convex Control Domain. By Ekeland’s variational principle and some basic estimates for state processes and adjoint processes, we establish the necessary conditions for any -near optimal Control in a local form with an error order of exact . Moreover, under additional convexity conditions on Hamiltonian function, we prove that an -maximum condition in terms of the Hamiltonian in the integral form is sufficient for near-optimality of order .
Takeshi Ogihara - One of the best experts on this subject based on the ideXlab platform.
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islet β cell specific mafa transcription requires the 5 flanking conserved region 3 Control Domain
Molecular and Cellular Biology, 2010Co-Authors: Jeffrey C Raum, Chad S Hunter, Isabella Artner, Eva Henderson, Lynda Elghazi, Beatriz Sosapineda, Takeshi Ogihara, Raghavendra G Mirmira, Lori SusselAbstract:MafA is a key transcriptional activator of islet beta cells and its exclusive expression within beta cells of the developing and adult pancreas is distinct amongst pancreatic regulators. Region 3 (base pairs -8118/-7750 relative to the transcription start site), one of six conserved 5' cis-Domains of the MafA promoter, is capable of directing beta-cell-line-selective expression. Transgenic reporters of Region 3 alone (R3), sequences spanning Regions 1-6 (R1-6; base pairs -10428/+230), and R1-6 lacking Region 3 (R1-6(DeltaR3)) were generated. Only the R1-6 transgene was active in MafA(+) insulin(+) cells during development and in adults. R1-6 also mediated glucose-induced MafA expression. Conversely, pancreatic expression was not observed with the R3 or R1-6(DeltaR3) lines, although much of the non-pancreatic expression pattern was shared between the R1-6 and R1-6(DeltaR3) lines. Further support for the importance of Region 3 was shown as the islet regulators Nkx6.1 and Pax6, but not NeuroD1 activated MafA using gel shift, chromatin immunoprecipitation (ChIP), transfection assays, and in vivo mouse knockout models. Lastly ChIP demonstrated that Pax6 and Pdx-1 bound also to Regions 1 and 6, potentially functioning in pancreatic and non-pancreatic expression. These data highlight the nature of the cis- and trans-acting factors Controlling the beta-3cell-specific expression of MafA. (Less)
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islet β cell specific mafa transcription requires the 5 flanking conserved region 3 Control Domain
Molecular and Cellular Biology, 2010Co-Authors: Jeffrey C Raum, Chad S Hunter, Isabella Artner, Eva Henderson, Lynda Elghazi, Beatriz Sosapineda, Takeshi Ogihara, Raghavendra G Mirmira, Min Guo, Lori SusselAbstract:MafA is a key transcriptional activator of islet beta cells, and its exclusive expression within beta cells of the developing and adult pancreas is distinct among pancreatic regulators. Region 3 (base pairs -8118 to -7750 relative to the transcription start site), one of six conserved 5' cis Domains of the MafA promoter, is capable of directing beta-cell-line-selective expression. Transgenic reporters of region 3 alone (R3), sequences spanning regions 1 to 6 (R1-6; base pairs -10428 to +230), and R1-6 lacking R3 (R1-6(DeltaR3)) were generated. Only the R1-6 transgene was active in MafA(+) insulin(+) cells during development and in adult cells. R1-6 also mediated glucose-induced MafA expression. Conversely, pancreatic expression was not observed with the R3 or R1-6(DeltaR3) line, although much of the nonpancreatic expression pattern was shared between the R1-6 and R1-6(DeltaR3) lines. Further support for the importance of R3 was also shown, as the islet regulators Nkx6.1 and Pax6, but not NeuroD1, activated MafA in gel shift, chromatin immunoprecipitation (ChIP), and transfection assays and in vivo mouse knockout models. Lastly, ChIP demonstrated that Pax6 and Pdx-1 also bound to R1 and R6, potentially functioning in pancreatic and nonpancreatic expression. These data highlight the nature of the cis- and trans-acting factors Controlling the beta-cell-specific expression of MafA.
Chad S Hunter - One of the best experts on this subject based on the ideXlab platform.
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islet β cell specific mafa transcription requires the 5 flanking conserved region 3 Control Domain
Molecular and Cellular Biology, 2010Co-Authors: Jeffrey C Raum, Chad S Hunter, Isabella Artner, Eva Henderson, Lynda Elghazi, Beatriz Sosapineda, Takeshi Ogihara, Raghavendra G Mirmira, Lori SusselAbstract:MafA is a key transcriptional activator of islet beta cells and its exclusive expression within beta cells of the developing and adult pancreas is distinct amongst pancreatic regulators. Region 3 (base pairs -8118/-7750 relative to the transcription start site), one of six conserved 5' cis-Domains of the MafA promoter, is capable of directing beta-cell-line-selective expression. Transgenic reporters of Region 3 alone (R3), sequences spanning Regions 1-6 (R1-6; base pairs -10428/+230), and R1-6 lacking Region 3 (R1-6(DeltaR3)) were generated. Only the R1-6 transgene was active in MafA(+) insulin(+) cells during development and in adults. R1-6 also mediated glucose-induced MafA expression. Conversely, pancreatic expression was not observed with the R3 or R1-6(DeltaR3) lines, although much of the non-pancreatic expression pattern was shared between the R1-6 and R1-6(DeltaR3) lines. Further support for the importance of Region 3 was shown as the islet regulators Nkx6.1 and Pax6, but not NeuroD1 activated MafA using gel shift, chromatin immunoprecipitation (ChIP), transfection assays, and in vivo mouse knockout models. Lastly ChIP demonstrated that Pax6 and Pdx-1 bound also to Regions 1 and 6, potentially functioning in pancreatic and non-pancreatic expression. These data highlight the nature of the cis- and trans-acting factors Controlling the beta-3cell-specific expression of MafA. (Less)
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islet β cell specific mafa transcription requires the 5 flanking conserved region 3 Control Domain
Molecular and Cellular Biology, 2010Co-Authors: Jeffrey C Raum, Chad S Hunter, Isabella Artner, Eva Henderson, Lynda Elghazi, Beatriz Sosapineda, Takeshi Ogihara, Raghavendra G Mirmira, Min Guo, Lori SusselAbstract:MafA is a key transcriptional activator of islet beta cells, and its exclusive expression within beta cells of the developing and adult pancreas is distinct among pancreatic regulators. Region 3 (base pairs -8118 to -7750 relative to the transcription start site), one of six conserved 5' cis Domains of the MafA promoter, is capable of directing beta-cell-line-selective expression. Transgenic reporters of region 3 alone (R3), sequences spanning regions 1 to 6 (R1-6; base pairs -10428 to +230), and R1-6 lacking R3 (R1-6(DeltaR3)) were generated. Only the R1-6 transgene was active in MafA(+) insulin(+) cells during development and in adult cells. R1-6 also mediated glucose-induced MafA expression. Conversely, pancreatic expression was not observed with the R3 or R1-6(DeltaR3) line, although much of the nonpancreatic expression pattern was shared between the R1-6 and R1-6(DeltaR3) lines. Further support for the importance of R3 was also shown, as the islet regulators Nkx6.1 and Pax6, but not NeuroD1, activated MafA in gel shift, chromatin immunoprecipitation (ChIP), and transfection assays and in vivo mouse knockout models. Lastly, ChIP demonstrated that Pax6 and Pdx-1 also bound to R1 and R6, potentially functioning in pancreatic and nonpancreatic expression. These data highlight the nature of the cis- and trans-acting factors Controlling the beta-cell-specific expression of MafA.