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D M Da Silva - One of the best experts on this subject based on the ideXlab platform.
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quality control of telenomus remus hymenoptera platygastridae reared on the factitious host Corcyra Cephalonica lepidoptera pyralidae for successive generations
Bulletin of Entomological Research, 2017Co-Authors: A P Queiroz, O C Bortolotto, A De F Bueno, Aline Pomarifernandes, M L M Grande, D M Da SilvaAbstract:Egg parasitoid rearing on factitious hosts is an important step to reduce costs and increase availability of the biocontrol agent but it requires quality control to achieve success in field conditions. To this end, this study evaluated the quality of Telenomus remus (Hymenoptera: Platygastridae) reared on Corcyra Cephalonica (Lepidoptera: Pyralidae) for until 45 generations. In the first bioassay, we evaluated the body size of the laboratory-produced parasitoids. In the second bioassay, flight activity was examined, measuring the percentage of ‘flyers’, ‘walkers’ and ‘deformed’ parasitoids. The third bioassay assessed parasitism on Spodoptera frugiperda (Lepidoptera: Noctuidae) eggs. Our data indicate that the laboratory-reared parasitoid neither lost its ability to fly nor to parasitize S. frugiperda eggs. In conclusion, quality did not decrease significantly during 45 generations, and therefore rearing of T. remus on C. Cephalonica as factitious host promises to be successful.
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low temperature storage of telenomus remus nixon hymenoptera platygastridae and its factitious host Corcyra Cephalonica stainton lepidoptera pyralidae
Neotropical Entomology, 2017Co-Authors: A P Queiroz, Adeney De Freitas Bueno, O C Bortolotto, Aline Pomarifernandes, M L M Grande, D M Da SilvaAbstract:We conducted three bioassays to evaluate the effect of low-temperature storage of eggs (host) and pupae and adults (parasitoid) on the biology and parasitism capacity of the egg parasitoid Telenomus remus (Nixon) (Hymenoptera: Platygastridae). Viable stored Corcyra Cephalonica (Stainton) (Lepidoptera: Pyralidae) eggs were parasitized to the same degree or even higher than fresh eggs when stored until 14 days at 5°C or until 21 days at 10°C. In contrast, the percentage of parasitized sterilized eggs was equal to the control only when stored for 7 and 14 days. Survival of T. remus pupae declined with storage time at both studied temperatures (5 and 10°C). However, after 7 days of storage, survival of pupae was still 86.3 and 64.9% at 10 and 5°C, respectively. The number of adult male survivors remained similar until the fourth storage day at both 5 and 10°C. In contrast, female survival did not differ until day 8 at 10°C or day 6 at 5°C. Parasitism capacity of stored adults was not altered by storage compared with the control. Therefore, we conclude that the maximal storage time at 10°C is 21 days for viable C. Cephalonica eggs and 7 days for T. remus pupae, while parasitoid adults should not be stored for more than 4 days at either 5 or 10°C.
A P Queiroz - One of the best experts on this subject based on the ideXlab platform.
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quality control of telenomus remus hymenoptera platygastridae reared on the factitious host Corcyra Cephalonica lepidoptera pyralidae for successive generations
Bulletin of Entomological Research, 2017Co-Authors: A P Queiroz, O C Bortolotto, A De F Bueno, Aline Pomarifernandes, M L M Grande, D M Da SilvaAbstract:Egg parasitoid rearing on factitious hosts is an important step to reduce costs and increase availability of the biocontrol agent but it requires quality control to achieve success in field conditions. To this end, this study evaluated the quality of Telenomus remus (Hymenoptera: Platygastridae) reared on Corcyra Cephalonica (Lepidoptera: Pyralidae) for until 45 generations. In the first bioassay, we evaluated the body size of the laboratory-produced parasitoids. In the second bioassay, flight activity was examined, measuring the percentage of ‘flyers’, ‘walkers’ and ‘deformed’ parasitoids. The third bioassay assessed parasitism on Spodoptera frugiperda (Lepidoptera: Noctuidae) eggs. Our data indicate that the laboratory-reared parasitoid neither lost its ability to fly nor to parasitize S. frugiperda eggs. In conclusion, quality did not decrease significantly during 45 generations, and therefore rearing of T. remus on C. Cephalonica as factitious host promises to be successful.
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low temperature storage of telenomus remus nixon hymenoptera platygastridae and its factitious host Corcyra Cephalonica stainton lepidoptera pyralidae
Neotropical Entomology, 2017Co-Authors: A P Queiroz, Adeney De Freitas Bueno, O C Bortolotto, Aline Pomarifernandes, M L M Grande, D M Da SilvaAbstract:We conducted three bioassays to evaluate the effect of low-temperature storage of eggs (host) and pupae and adults (parasitoid) on the biology and parasitism capacity of the egg parasitoid Telenomus remus (Nixon) (Hymenoptera: Platygastridae). Viable stored Corcyra Cephalonica (Stainton) (Lepidoptera: Pyralidae) eggs were parasitized to the same degree or even higher than fresh eggs when stored until 14 days at 5°C or until 21 days at 10°C. In contrast, the percentage of parasitized sterilized eggs was equal to the control only when stored for 7 and 14 days. Survival of T. remus pupae declined with storage time at both studied temperatures (5 and 10°C). However, after 7 days of storage, survival of pupae was still 86.3 and 64.9% at 10 and 5°C, respectively. The number of adult male survivors remained similar until the fourth storage day at both 5 and 10°C. In contrast, female survival did not differ until day 8 at 10°C or day 6 at 5°C. Parasitism capacity of stored adults was not altered by storage compared with the control. Therefore, we conclude that the maximal storage time at 10°C is 21 days for viable C. Cephalonica eggs and 7 days for T. remus pupae, while parasitoid adults should not be stored for more than 4 days at either 5 or 10°C.
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the importance of relative humidity for telenomus remus hymenoptera platygastridae parasitism and development on Corcyra Cephalonica lepidoptera pyralidae and spodoptera frugiperda lepidoptera noctuidae eggs
Annals of The Entomological Society of America, 2015Co-Authors: Aline Pomarifernandes, Adeney De Freitas Bueno, A P Queiroz, Allisson Wilson Sanzovo, Sergio Antonio De BortoliAbstract:The rearing of Telenomus remus (Nixon 1937) has been limited due to the difficulty of its development in its natural host, the eggs of Spodoptera frugiperda (J.E. Smith). Environmental conditions can strongly affect egg’s parasitoid rearing on factitious hosts; however, until now only the effects of temperature have been studied. The importance of relative humidity in T. remus parasitism is poorly understood; therefore, this study evaluated its parasitism on Corcyra Cephalonica (Stainton, 1866) and S. frugiperda eggs under different humidity regimes. Lifetime parasitism and parasitism viability were higher on S. frugiperda eggs and did not differ among humidity regimes. However, these biological parameters were higher at 80% humidity than at both 40 and 60% humidity when T. remus was reared on C. Cephalonica eggs. The sex ratio was always higher than 0.6 for all hosts and humidity regimes. Parental female longevity was only influenced by humidity when presented to C. Cephalonica eggs: it was higher at 60 and 80% humidity. T. remus egg-to-adult period was longer in C. Cephalonica eggs; however, no difference between humidity regimes was noted. In general, relative humidity affected T. remus development only when it was reared on C. Cephalonica eggs. For this factitious host, 80% humidity was shown to be appropriate for egg parasitoid rearing due to its better biological performance.
Aline Pomarifernandes - One of the best experts on this subject based on the ideXlab platform.
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quality control of telenomus remus hymenoptera platygastridae reared on the factitious host Corcyra Cephalonica lepidoptera pyralidae for successive generations
Bulletin of Entomological Research, 2017Co-Authors: A P Queiroz, O C Bortolotto, A De F Bueno, Aline Pomarifernandes, M L M Grande, D M Da SilvaAbstract:Egg parasitoid rearing on factitious hosts is an important step to reduce costs and increase availability of the biocontrol agent but it requires quality control to achieve success in field conditions. To this end, this study evaluated the quality of Telenomus remus (Hymenoptera: Platygastridae) reared on Corcyra Cephalonica (Lepidoptera: Pyralidae) for until 45 generations. In the first bioassay, we evaluated the body size of the laboratory-produced parasitoids. In the second bioassay, flight activity was examined, measuring the percentage of ‘flyers’, ‘walkers’ and ‘deformed’ parasitoids. The third bioassay assessed parasitism on Spodoptera frugiperda (Lepidoptera: Noctuidae) eggs. Our data indicate that the laboratory-reared parasitoid neither lost its ability to fly nor to parasitize S. frugiperda eggs. In conclusion, quality did not decrease significantly during 45 generations, and therefore rearing of T. remus on C. Cephalonica as factitious host promises to be successful.
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low temperature storage of telenomus remus nixon hymenoptera platygastridae and its factitious host Corcyra Cephalonica stainton lepidoptera pyralidae
Neotropical Entomology, 2017Co-Authors: A P Queiroz, Adeney De Freitas Bueno, O C Bortolotto, Aline Pomarifernandes, M L M Grande, D M Da SilvaAbstract:We conducted three bioassays to evaluate the effect of low-temperature storage of eggs (host) and pupae and adults (parasitoid) on the biology and parasitism capacity of the egg parasitoid Telenomus remus (Nixon) (Hymenoptera: Platygastridae). Viable stored Corcyra Cephalonica (Stainton) (Lepidoptera: Pyralidae) eggs were parasitized to the same degree or even higher than fresh eggs when stored until 14 days at 5°C or until 21 days at 10°C. In contrast, the percentage of parasitized sterilized eggs was equal to the control only when stored for 7 and 14 days. Survival of T. remus pupae declined with storage time at both studied temperatures (5 and 10°C). However, after 7 days of storage, survival of pupae was still 86.3 and 64.9% at 10 and 5°C, respectively. The number of adult male survivors remained similar until the fourth storage day at both 5 and 10°C. In contrast, female survival did not differ until day 8 at 10°C or day 6 at 5°C. Parasitism capacity of stored adults was not altered by storage compared with the control. Therefore, we conclude that the maximal storage time at 10°C is 21 days for viable C. Cephalonica eggs and 7 days for T. remus pupae, while parasitoid adults should not be stored for more than 4 days at either 5 or 10°C.
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size and flight ability of telenomus remus parasitoids reared on eggs of the factitious host Corcyra Cephalonica
Revista Brasileira De Entomologia, 2016Co-Authors: Aline Pomarifernandes, Adeney De Freitas Bueno, Sergio Antonio De BortoliAbstract:In two independent bioassays, size and flight ability of parasitoids reared on eggs of Corcyra Cephalonica for 19 generations and parasitoids reared on a natural host (Spodoptera frugiperda eggs) for 250 generations were compared as fast quality control procedures for insect rearing. The size of parasitoids was examined by morphometric analysis using a stereoscope. Length and width of the wings, right hind tibia, and the body of 20 individuals (males and females) were measured. In the analysis of flight ability, parasitoids were divided into three groups: individuals able to fly ("flyers"), individuals that did not fly but had no visible deformation ("walkers"), and individuals with visible deformation ("deformed"). We observed that parasitoids were larger when reared on the natural host than on the factitious host for all evaluated morphological characters. However, there was no significant difference between the treatments regarding the number of "flyers", "walkers" or "deformed" parasitoids. This indicates that even though the rearing of T. remus on a factitious host affects parasitoid size, it does not necessarily affect its flight ability and therefore suggests that C. Cephalonica is suitable as a factitious host for mass rearing of T. remus. Other biological parameters still need to be evaluated, such as host finding ability, parasitism capacity, and parasitoid field efficacy in order to provide a more complete picture of the effects caused by a host change. However, because fast laboratory tests are needed in rearing facilities, the one used in this study might be useful to rapidly assess parasitoid quality.
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the importance of relative humidity for telenomus remus hymenoptera platygastridae parasitism and development on Corcyra Cephalonica lepidoptera pyralidae and spodoptera frugiperda lepidoptera noctuidae eggs
Annals of The Entomological Society of America, 2015Co-Authors: Aline Pomarifernandes, Adeney De Freitas Bueno, A P Queiroz, Allisson Wilson Sanzovo, Sergio Antonio De BortoliAbstract:The rearing of Telenomus remus (Nixon 1937) has been limited due to the difficulty of its development in its natural host, the eggs of Spodoptera frugiperda (J.E. Smith). Environmental conditions can strongly affect egg’s parasitoid rearing on factitious hosts; however, until now only the effects of temperature have been studied. The importance of relative humidity in T. remus parasitism is poorly understood; therefore, this study evaluated its parasitism on Corcyra Cephalonica (Stainton, 1866) and S. frugiperda eggs under different humidity regimes. Lifetime parasitism and parasitism viability were higher on S. frugiperda eggs and did not differ among humidity regimes. However, these biological parameters were higher at 80% humidity than at both 40 and 60% humidity when T. remus was reared on C. Cephalonica eggs. The sex ratio was always higher than 0.6 for all hosts and humidity regimes. Parental female longevity was only influenced by humidity when presented to C. Cephalonica eggs: it was higher at 60 and 80% humidity. T. remus egg-to-adult period was longer in C. Cephalonica eggs; however, no difference between humidity regimes was noted. In general, relative humidity affected T. remus development only when it was reared on C. Cephalonica eggs. For this factitious host, 80% humidity was shown to be appropriate for egg parasitoid rearing due to its better biological performance.
Aparna Dutta-gupta - One of the best experts on this subject based on the ideXlab platform.
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Identification of 86 kDa protein as methionine rich hexamerin in the rice moth, Corcyra Cephalonica
Comparative biochemistry and physiology. Part B Biochemistry & molecular biology, 2010Co-Authors: Manohar Damara, Aparna Dutta-guptaAbstract:We cloned the complete cDNA of methionine rich hexamerin from rice moth, Corcyra Cephalonica using RACE strategy. The amplicon size was 2.5 kb with an ORF of 2.31 kb. The cDNA clone showed high percentage of methionine (4.8%), which is consistent with the previously reported sequences in other insects. The Southern and Northern analysis carried out showed that methionine rich hexamerin in rice moth is a single copy gene. Multiple alignment analysis of amino acid sequence revealed that the cDNA clone is most similar to Plodia interpunctella hexamerin storage protein (74% identity). The calculated isoelectric point is 9.2. The deduced amino acid sequence corresponded to 86 kDa subunit of hexamerin protein in rice moth. The 86 kDa protein (methionine rich subunit) was purified and polyclonal antibodies were raised against the subunit to check the specificity of the purified subunit. The developmental profile of 86 kDa subunit during the larval stages both in the fat body and haemolymph show that it is present at a higher concentration during the LLI (late-last instar) larval stage compared to the previous stages. The present work carried out shows that the methionine rich hexamerin cloned is the 86 kDa subunit of hexamerin which was identified previously by our group in rice moth, C. Cephalonica.
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Ecdysteroid-mediated expression of hexamerin (arylphorin) in the rice moth, Corcyra Cephalonica.
Journal of insect physiology, 2010Co-Authors: Damara Manohar, Damodar Gullipalli, Aparna Dutta-guptaAbstract:Abstract The insect development is intricately controlled by morphogenetic hormones, juvenile hormone (JH) and 20-hydroxyecdysone (20E) through the regulation of gene/protein expression. The role of hexamerins in the metamorphosis of insects and reproduction and their control by 20E at the gene level has been widely reported in insects. In the present study we for the first time report the role of ecdysteroids in the regulation of hexamerin synthesis in a lepidopteran insect Corcyra Cephalonica . The hormonal studies were carried out using the normal and the thorax-ligated insects with both 20E and its non-steroidal agonist RH-5992. The in vitro as well as in vivo studies showed a stimulatory effect of 20E and its agonist on the hexamerin synthesis including arylphorin (Hex 2), whereas hormone blockade with azadirachtin caused a time dependent reduction in synthesis. The northern analysis using Hex 2b cDNA as probe too confirmed the above result. This was followed by the cloning of the Hex 2b gene. The full length of the genomic clone was found to be 3.5 kb long and has four exons interspersed by three introns. The genome walking analysis revealed the presence of a steroid hormone binding sequence “Ecdysone response element” (EcRE) in the 5′ untranscribed region (UTR) of the gene. The data presented in this paper clearly suggest that hexamerin synthesis in C. Cephalonica is transcriptionally regulated by 20E.
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Cloning and expression of fat body hexamerin receptor and its identification in other hexamerin sequestering tissue of rice moth, Corcyra Cephalonica.
Journal of insect physiology, 2010Co-Authors: Manohar Damara, Damodar Gullipalli, Aparna Dutta-guptaAbstract:Abstract Selective receptor mediated uptake is a widely prevalent mechanism in insects by which important macromolecules are acquired. Among the various proteins sequestered by the insect fat body, the larval hexamerins form the major group. In the present work full length cDNA (2.6 kb) of hexamerin receptor with an ORF of 2.4 kb was cloned from the larval fat body of rice moth, Corcyra Cephalonica. This was followed by the recombinant expression of truncated N-terminal sequence of putative hexamerin receptor and the confirmation of the expressed recombinant protein as the truncated hexamerin receptor by ligand blot analysis. Apart from this we also analyzed other hexamerin sequestering tissues like salivary gland, male accessory reproductive gland and ovary for the presence of hexamerin receptor. We found that the receptor in these tissues was similar in size and mode of activation to that of fat body hexamerin receptor, thus cementing the fact that identical hexamerin receptors are present in all the hexamerin sequestering tissues in the rice moth.
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Regulation of hexamerin receptor phosphorylation by hemolymph protein HP19 and 20-hydroxyecdysone directs hexamerin uptake in the rice moth Corcyra Cephalonica.
Insect biochemistry and molecular biology, 2007Co-Authors: Abul Arif, Damodar Gullipalli, Damara Manohar, Aparna Dutta-guptaAbstract:Hexamerins are stage specifically sequestered during the non-feeding stages mainly by the fat body cells from hemolymph through ecdysteroid regulated receptor-mediated endocytosis. 20-Hydroxyecdysone (20E) stimulates the tyrosine kinase-mediated phosphorylation of the 120 kDa hexamerin receptor in the rice moth, Corcyra Cephalonica. Here, we demonstrate that phosphorylation of the hexamerin receptor by HP19-regulated-20E-dependent-tyrosine kinase is a critical regulator for its activation, and is required for hexamerin uptake. Hexamerin receptor is phosphorylated only in the hexamerin sequestering tissues. The receptor phosphorylation is a prerequisite for hexamerin uptake and both phosphorylation and concomitant uptake are developmentally regulated. In addition, endogenous fat body tyrosine kinase activity is also developmentally and hormonally regulated. 20E induces the tyrosine kinase activity both in vivo as well as ex vivo, and the receptor phosphorylation is likely an extra-cellular event. The hemolymph protein, HP19 inhibits the 20E-induced phosphorylation by inhibiting tyrosine kinase activity. These inhibitions are rapid in homogenate preparations and are unaffected by the inhibitors of transcription and translation. We propose that hexamerin sequestration is negatively regulated by active HP19 at the feeding larval stage, thus preventing the uptake. During the non-feeding pupal stage, high ecdysteroid titer and negligible HP19 activity, positively regulates the receptor phosphorylation resulting in hexamerin uptake. These studies are therefore the first evidence of hexamerin uptake regulated by the orchestration of 20E and HP19 at a nongenomic level.
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Tyrosine kinase mediated phosphorylation of the hexamerin receptor in the rice moth Corcyra Cephalonica by ecdysteroids.
Insect biochemistry and molecular biology, 2003Co-Authors: Abul Arif, Klaus Scheller, Aparna Dutta-guptaAbstract:Hexamerins are multifunctional insect storage proteins utilized during metamorphosis of holometabolous insects. These proteins are stage specifically taken up by the fat body cells from the haemolymph due to receptor-mediated endocytosis. The hexamerin receptor and the concomitant hexamerin sequestration in the rice moth Corcyra Cephalonica is controlled by the steroid hormone 20-hydroxy-ecdysone (20E). However, the mechanism of receptor activation for hexamerin uptake is not yet clear. We report here that 20E stimulates the phosphorylation of 120 kDa hexamerin binding protein which has been demonstrated to represent the receptor. Phosphorylation of the receptor is suggested to be essential for receptor activation and occurs prior to the hexamerin uptake. The 20E stimulated phosphorylation is mediated partly by a tyrosine kinase as phosphotyrosine antibodies cross-react with the receptor and its phosphorylation is blocked partly by genistein. Back phosphorylation study provides additional evidence for 20E regulation of hexamerin receptor phosphorylation in intact fat body. The receptor phosphorylation is developmentally regulated. This is the first report demonstrating that (i) the uptake of hexamerin is dependent on the phosphorylation of hexamerin receptor and (ii) the phosphorylation is catalyzed partly by a tyrosine kinase which is activated by 20E through a non-genomic action.
Sergio Antonio De Bortoli - One of the best experts on this subject based on the ideXlab platform.
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Cost of Production of Telenomus remus (Hymenoptera: Platygastridae) Grown in Natural and Alternative Hosts.
Journal of economic entomology, 2017Co-Authors: Natalia Fernanda Vieira, Aline Pomari-fernandes, Amanda A F Lemes, Alessandra Marieli Vacari, Sergio Antonio De Bortoli, Adeney De Freitas BuenoAbstract:The study of the technical and economic aspects of rearing natural enemies is essential for its effective use as a biological control agent in the field. The aim of this study was to determine the cost of production of the parasitoid, Telenomus remus (Nixon; Hymenoptera: Platygastridae), reared in eggs of its natural host, Spodoptera frugiperda (J. E. Smith; Lepidoptera: Noctuidae), and the alternative host, Corcyra Cephalonica (Stainton; Lepidoptera: Pyralidae). The insects were reared in accordance with their respective methodologies of laboratory rearing. The cost of production of this biocontrol agent is US$ 0.0004 when reared with S. frugiperda eggs and US$ 0.0002 with C. Cephalonica eggs.
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size and flight ability of telenomus remus parasitoids reared on eggs of the factitious host Corcyra Cephalonica
Revista Brasileira De Entomologia, 2016Co-Authors: Aline Pomarifernandes, Adeney De Freitas Bueno, Sergio Antonio De BortoliAbstract:In two independent bioassays, size and flight ability of parasitoids reared on eggs of Corcyra Cephalonica for 19 generations and parasitoids reared on a natural host (Spodoptera frugiperda eggs) for 250 generations were compared as fast quality control procedures for insect rearing. The size of parasitoids was examined by morphometric analysis using a stereoscope. Length and width of the wings, right hind tibia, and the body of 20 individuals (males and females) were measured. In the analysis of flight ability, parasitoids were divided into three groups: individuals able to fly ("flyers"), individuals that did not fly but had no visible deformation ("walkers"), and individuals with visible deformation ("deformed"). We observed that parasitoids were larger when reared on the natural host than on the factitious host for all evaluated morphological characters. However, there was no significant difference between the treatments regarding the number of "flyers", "walkers" or "deformed" parasitoids. This indicates that even though the rearing of T. remus on a factitious host affects parasitoid size, it does not necessarily affect its flight ability and therefore suggests that C. Cephalonica is suitable as a factitious host for mass rearing of T. remus. Other biological parameters still need to be evaluated, such as host finding ability, parasitism capacity, and parasitoid field efficacy in order to provide a more complete picture of the effects caused by a host change. However, because fast laboratory tests are needed in rearing facilities, the one used in this study might be useful to rapidly assess parasitoid quality.
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Size and flight ability of Telenomus remus parasitoids reared on eggs of the factitious host Corcyra Cephalonica
Elsevier, 2016Co-Authors: Aline Pomari-fernandes, Adeney De Freitas Bueno, Sergio Antonio De BortoliAbstract:In two independent bioassays, size and flight ability of parasitoids reared on eggs of Corcyra Cephalonica for 19 generations and parasitoids reared on a natural host (Spodoptera frugiperda eggs) for 250 generations were compared as fast quality control procedures for insect rearing. The size of parasitoids was examined by morphometric analysis using a stereoscope. Length and width of the wings, right hind tibia, and the body of 20 individuals (males and females) were measured. In the analysis of flight ability, parasitoids were divided into three groups: individuals able to fly (“flyers”), individuals that did not fly but had no visible deformation (“walkers”), and individuals with visible deformation (“deformed”). We observed that parasitoids were larger when reared on the natural host than on the factitious host for all evaluated morphological characters. However, there was no significant difference between the treatments regarding the number of “flyers”, “walkers” or “deformed” parasitoids. This indicates that even though the rearing of T. remus on a factitious host affects parasitoid size, it does not necessarily affect its flight ability and therefore suggests that C. Cephalonica is suitable as a factitious host for mass rearing of T. remus. Other biological parameters still need to be evaluated, such as host finding ability, parasitism capacity, and parasitoid field efficacy in order to provide a more complete picture of the effects caused by a host change. However, because fast laboratory tests are needed in rearing facilities, the one used in this study might be useful to rapidly assess parasitoid quality. Keywords: Egg parasitoid, Insect mass rearing, Platygastridae, Natural enem
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the importance of relative humidity for telenomus remus hymenoptera platygastridae parasitism and development on Corcyra Cephalonica lepidoptera pyralidae and spodoptera frugiperda lepidoptera noctuidae eggs
Annals of The Entomological Society of America, 2015Co-Authors: Aline Pomarifernandes, Adeney De Freitas Bueno, A P Queiroz, Allisson Wilson Sanzovo, Sergio Antonio De BortoliAbstract:The rearing of Telenomus remus (Nixon 1937) has been limited due to the difficulty of its development in its natural host, the eggs of Spodoptera frugiperda (J.E. Smith). Environmental conditions can strongly affect egg’s parasitoid rearing on factitious hosts; however, until now only the effects of temperature have been studied. The importance of relative humidity in T. remus parasitism is poorly understood; therefore, this study evaluated its parasitism on Corcyra Cephalonica (Stainton, 1866) and S. frugiperda eggs under different humidity regimes. Lifetime parasitism and parasitism viability were higher on S. frugiperda eggs and did not differ among humidity regimes. However, these biological parameters were higher at 80% humidity than at both 40 and 60% humidity when T. remus was reared on C. Cephalonica eggs. The sex ratio was always higher than 0.6 for all hosts and humidity regimes. Parental female longevity was only influenced by humidity when presented to C. Cephalonica eggs: it was higher at 60 and 80% humidity. T. remus egg-to-adult period was longer in C. Cephalonica eggs; however, no difference between humidity regimes was noted. In general, relative humidity affected T. remus development only when it was reared on C. Cephalonica eggs. For this factitious host, 80% humidity was shown to be appropriate for egg parasitoid rearing due to its better biological performance.
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The importance of relative humidity for telenomus remus (Hymenoptera: Platygastridae) parasitism and development on Corcyra Cephalonica (Lepidoptera: Pyralidae) and Spodoptera frugiperda (Lepidoptera: Noctuidae) eggs
Oxford Univ Press Inc, 2015Co-Authors: Pomari-fernandes Aline, Ana Paula De ,queiroz, Bueno, Adeney De Freitas, Sanzovo, Allisson Wilson, Sergio Antonio De BortoliAbstract:The rearing of Telenomus remus (Nixon 1937) has been limited due to the difficulty of its development in its natural host, the eggs of Spodoptera frugiperda (J.E. Smith). Environmental conditions can strongly affect egg's parasitoid rearing on factitious hosts; however, until now only the effects of temperature have been studied. The importance of relative humidity in T. remus parasitism is poorly understood; therefore, this study evaluated its parasitism on Corcyra Cephalonica (Stainton, 1866) and S. frugiperda eggs under different humidity regimes. Lifetime parasitism and parasitism viability were higher on S. frugiperda eggs and did not differ among humidity regimes. However, these biological parameters were higher at 80% humidity than at both 40 and 60% humidity when T. remus was reared on C. Cephalonica eggs. The sex ratio was always higher than 0.6 for all hosts and humidity regimes. Parental female longevity was only influenced by humidity when presented to C. Cephalonica eggs: it was higher at 60 and 80% humidity. T. remus egg-to-adult period was longer in C. Cephalonica eggs; however, no difference between humidity regimes was noted. In general, relative humidity affected T. remus development only when it was reared on C. Cephalonica eggs. For this factitious host, 80% humidity was shown to be appropriate for egg parasitoid rearing due to its better biological performance.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq