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Czeslaw Lugowski - One of the best experts on this subject based on the ideXlab platform.
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The New Structure of Core Oligosaccharide Presented by Proteus penneri 40A and 41 Lipopolysaccharides
MDPI AG, 2018Co-Authors: Agata Palusiak, Czeslaw Lugowski, Anna Maciejewska, Antoni Rozalski, Marta KaszowskaAbstract:The new type of Core Oligosaccharide in Proteus penneri 40A and 41 lipopolysaccharides has been investigated by 1H and 13C NMR spectroscopy, electrospray ionization mass spectrometry and chemical methods. Core Oligosaccharides of both strains were chosen for structural analysis based on the reactivity of LPSs with serum against P. penneri 40A Core Oligosaccharide–diphtheria toxoid conjugate. Structural analyses revealed that P. penneri 40A and 41 LPSs possess an identical Core Oligosaccharide
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The Complete Structure of the Core Oligosaccharide from Edwardsiella tarda EIB 202 Lipopolysaccharide
MDPI AG, 2017Co-Authors: Marta Kaszowska, Anna Maciejewska, Czeslaw Lugowski, Susana Merino, Elena De Mendoza-barberá, Juan M. TomásAbstract:The chemical structure and genomics of the lipopolysaccharide (LPS) Core Oligosaccharide of pathogenic Edwardsiella tarda strain EIB 202 were studied for the first time. The complete gene assignment for all LPS Core biosynthesis gene functions was acquired. The complete structure of Core Oligosaccharide was investigated by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy, electrospray ionization mass spectrometry MSn, and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry. The following structure of the undecasaccharide was established: The heterogeneous appearance of the Core Oligosaccharide structure was due to the partial lack of β-d-Galp and the replacement of α-d-GlcpNAcGly by α-d-GlcpNGly. The glycine location was identified by mass spectrometry
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Core Oligosaccharide of escherichia coli b the structure required for bacteriophage t4 recognition
Carbohydrate Research, 2015Co-Authors: Marta Kaszowska, Tomasz Niedziela, Anna Maciejewska, Jolanta Lukasiewicz, Wojciech Jachymek, Czeslaw LugowskiAbstract:Abstract The structure of Escherichia coli B strain PCM 1935 Core Oligosaccharide has been investigated by 1H and 13C NMR spectroscopy, MALDI-TOF MS and ESI MSn. It was concluded that the Core Oligosaccharide is a pentasaccharide with the following structure: ESI MS/MS analysis revealed that the glycine (a minor component) is linked to the →3,7)- l -α- d -Hepp-(1→ residue.
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occurrence of glycine in the Core Oligosaccharides of hafnia alvei lipopolysaccharides identification of disubstituted glycoform
Carbohydrate Research, 2015Co-Authors: Tomasz K Gozdziewicz, Aleksandra Mankupisinska, Czeslaw Lugowski, Jolanta LukasiewiczAbstract:Endotoxins (lipopolysaccharides, LPS) are the main surface antigens and virulence factors of Gram-negative bacteria involved for example in the development of nosocomial infections and sepsis. They consist of three main regions: O-specific polysaccharide, Core Oligosaccharide, and lipid A. Bacteria modify LPS structure to escape the immune defence, but also to adapt to environmental conditions. LPS's structures are highly diversified in the O-specific polysaccharide region to evade bactericidal factors of immune system, but retain some common epitopes that are potential candidates for therapeutic strategies against bacterial infections. Common occurrence of glycine within the structure of LPS is a known phenomenon and was previously reported for variety of species. Since glycine residue substitutes mainly Core Oligosaccharide of LPS, especially inner Core region, it was also considered as a part of common epitope for broad-reactive antimicrobial antibodies. Herein, we used multiple-stage electrospray ionisation mass spectrometry to identify glycine substitution in Core Oligosaccharide type characteristic for Hafnia alvei LPS, and isolated from five strains of different O-serotypes: 32, PCM 1190, PCM 1192, PCM 1200, and PCM 1209. The location of glycine in Core Oligosaccharide was determined in detail for LPS 1190 using ESI-MS(n). Three glycoforms were identified, including two mono-glycinylated and one diglycinylated Core Oligosaccharides.
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Core Oligosaccharide of Escherichia coli B—the structure required for bacteriophage T4 recognition
Carbohydrate Research, 2015Co-Authors: Marta Kaszowska, Tomasz Niedziela, Anna Maciejewska, Jolanta Lukasiewicz, Wojciech Jachymek, Czeslaw LugowskiAbstract:Abstract The structure of Escherichia coli B strain PCM 1935 Core Oligosaccharide has been investigated by 1H and 13C NMR spectroscopy, MALDI-TOF MS and ESI MSn. It was concluded that the Core Oligosaccharide is a pentasaccharide with the following structure: ESI MS/MS analysis revealed that the glycine (a minor component) is linked to the →3,7)- l -α- d -Hepp-(1→ residue.
Jolanta Lukasiewicz - One of the best experts on this subject based on the ideXlab platform.
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Core Oligosaccharide of escherichia coli b the structure required for bacteriophage t4 recognition
Carbohydrate Research, 2015Co-Authors: Marta Kaszowska, Tomasz Niedziela, Anna Maciejewska, Jolanta Lukasiewicz, Wojciech Jachymek, Czeslaw LugowskiAbstract:Abstract The structure of Escherichia coli B strain PCM 1935 Core Oligosaccharide has been investigated by 1H and 13C NMR spectroscopy, MALDI-TOF MS and ESI MSn. It was concluded that the Core Oligosaccharide is a pentasaccharide with the following structure: ESI MS/MS analysis revealed that the glycine (a minor component) is linked to the →3,7)- l -α- d -Hepp-(1→ residue.
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occurrence of glycine in the Core Oligosaccharides of hafnia alvei lipopolysaccharides identification of disubstituted glycoform
Carbohydrate Research, 2015Co-Authors: Tomasz K Gozdziewicz, Aleksandra Mankupisinska, Czeslaw Lugowski, Jolanta LukasiewiczAbstract:Endotoxins (lipopolysaccharides, LPS) are the main surface antigens and virulence factors of Gram-negative bacteria involved for example in the development of nosocomial infections and sepsis. They consist of three main regions: O-specific polysaccharide, Core Oligosaccharide, and lipid A. Bacteria modify LPS structure to escape the immune defence, but also to adapt to environmental conditions. LPS's structures are highly diversified in the O-specific polysaccharide region to evade bactericidal factors of immune system, but retain some common epitopes that are potential candidates for therapeutic strategies against bacterial infections. Common occurrence of glycine within the structure of LPS is a known phenomenon and was previously reported for variety of species. Since glycine residue substitutes mainly Core Oligosaccharide of LPS, especially inner Core region, it was also considered as a part of common epitope for broad-reactive antimicrobial antibodies. Herein, we used multiple-stage electrospray ionisation mass spectrometry to identify glycine substitution in Core Oligosaccharide type characteristic for Hafnia alvei LPS, and isolated from five strains of different O-serotypes: 32, PCM 1190, PCM 1192, PCM 1200, and PCM 1209. The location of glycine in Core Oligosaccharide was determined in detail for LPS 1190 using ESI-MS(n). Three glycoforms were identified, including two mono-glycinylated and one diglycinylated Core Oligosaccharides.
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Core Oligosaccharide of Escherichia coli B—the structure required for bacteriophage T4 recognition
Carbohydrate Research, 2015Co-Authors: Marta Kaszowska, Tomasz Niedziela, Anna Maciejewska, Jolanta Lukasiewicz, Wojciech Jachymek, Czeslaw LugowskiAbstract:Abstract The structure of Escherichia coli B strain PCM 1935 Core Oligosaccharide has been investigated by 1H and 13C NMR spectroscopy, MALDI-TOF MS and ESI MSn. It was concluded that the Core Oligosaccharide is a pentasaccharide with the following structure: ESI MS/MS analysis revealed that the glycine (a minor component) is linked to the →3,7)- l -α- d -Hepp-(1→ residue.
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structural analysis of the Core Oligosaccharide and the o specific polysaccharide from the plesiomonas shigelloides o33 h3 strain cnctc 34 89 lipopolysaccharide
European Journal of Organic Chemistry, 2014Co-Authors: Gustav Nestor, Jolanta Lukasiewicz, Corine SandströmAbstract:Lipopolysaccharides (LPS, endotoxin) with different Core Oligosaccharide glycoforms from the same species are common, but diversity among glycoforms reported to date has been related to terminal residues only. In this work, contrary to the variety of known LPS structures, we observed a 30 % replacement of glucosamine to glucose in the middle of the outer Core region of the Plesiomonas shigelloides serotype O33:H3 (CNCTC 34/89) lipopolysaccharide. Such atypical modification within the same serotype raises questions about the biosynthesis of Core structures. The Core Oligosaccharide was an undecasaccharide with a high level of O-acetylation on the residue linked to the O-specific polysaccharide. The Core Oligosaccharide, the O-specific polysaccharide, and the linkage between them were determined by 1H and 13C NMR spectroscopy, mass spectrometry and chemical analysis. The presence of the O-specific polysaccharide on the bacterial cell surface was confirmed by 1H high-resolution magic angle spinning NMR spectroscopy.
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Structural Analysis of the Core Oligosaccharide and the O‐Specific Polysaccharide from the Plesiomonas shigelloides O33:H3 (Strain CNCTC 34/89) Lipopolysaccharide
European Journal of Organic Chemistry, 2013Co-Authors: Gustav Nestor, Jolanta Lukasiewicz, Corine SandströmAbstract:Lipopolysaccharides (LPS, endotoxin) with different Core Oligosaccharide glycoforms from the same species are common, but diversity among glycoforms reported to date has been related to terminal residues only. In this work, contrary to the variety of known LPS structures, we observed a 30 % replacement of glucosamine to glucose in the middle of the outer Core region of the Plesiomonas shigelloides serotype O33:H3 (CNCTC 34/89) lipopolysaccharide. Such atypical modification within the same serotype raises questions about the biosynthesis of Core structures. The Core Oligosaccharide was an undecasaccharide with a high level of O-acetylation on the residue linked to the O-specific polysaccharide. The Core Oligosaccharide, the O-specific polysaccharide, and the linkage between them were determined by 1H and 13C NMR spectroscopy, mass spectrometry and chemical analysis. The presence of the O-specific polysaccharide on the bacterial cell surface was confirmed by 1H high-resolution magic angle spinning NMR spectroscopy.
Tomasz Niedziela - One of the best experts on this subject based on the ideXlab platform.
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Bordetella holmesii Lipopolysaccharide Hide and Seek Game with Pertussis: Structural Analysis of the O-Specific Polysaccharide and the Core Oligosaccharide of the Type Strain ATCC 51541.
International journal of molecular sciences, 2020Co-Authors: Karolina Ucieklak, Sabina Koj, Tomasz NiedzielaAbstract:Whooping cough is a highly contagious disease caused predominantly by Bordetella pertussis, but it also comprises of a pertussis-like illness caused by B. holmesii. The virulence factors of B. holmesii and their role in the pathogenesis remain unknown. Lipopolysaccharide is the main surface antigen of all Bordetellae. Data on the structural features of the lipopolysaccharide (LPS) of B. holmesii are scarce. The poly- and Oligosaccharide components released by mild acidic hydrolysis of the LPS were separated and investigated by 1H and 13C NMR spectroscopy, mass spectrometry, and chemical methods. The structures of the O-specific polysaccharide and the Core Oligosaccharide of B. holmesii ATCC 51541 have been identified for the first time. The novel pentasaccharide repeating unit of the B. holmesii O-specific polysaccharide has the following structure: {→2)-α-l-Rhap-(1→6)-α-d-Glcp-(1→4)-[β-d-GlcpNAc-(1→3]-α-d-Galp-(1→3)-α-d-GlcpNAc-(1→}n. The SDS-PAGE and serological cross-reactivities of the B. holmesii LPS suggested the similarity between the Core Oligosaccharides of B. holmesii ATCC 51541 and B. pertussis strain 606. The main Oligosaccharide fraction contained a nonasaccharide. The comparative analysis of the NMR spectra of B. holmesii Core Oligosaccharide fraction with this of the B. pertussis strain 606 indicated that the investigated Core Oligosaccharides were identical.
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Core Oligosaccharide of escherichia coli b the structure required for bacteriophage t4 recognition
Carbohydrate Research, 2015Co-Authors: Marta Kaszowska, Tomasz Niedziela, Anna Maciejewska, Jolanta Lukasiewicz, Wojciech Jachymek, Czeslaw LugowskiAbstract:Abstract The structure of Escherichia coli B strain PCM 1935 Core Oligosaccharide has been investigated by 1H and 13C NMR spectroscopy, MALDI-TOF MS and ESI MSn. It was concluded that the Core Oligosaccharide is a pentasaccharide with the following structure: ESI MS/MS analysis revealed that the glycine (a minor component) is linked to the →3,7)- l -α- d -Hepp-(1→ residue.
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Core Oligosaccharide of Escherichia coli B—the structure required for bacteriophage T4 recognition
Carbohydrate Research, 2015Co-Authors: Marta Kaszowska, Tomasz Niedziela, Anna Maciejewska, Jolanta Lukasiewicz, Wojciech Jachymek, Czeslaw LugowskiAbstract:Abstract The structure of Escherichia coli B strain PCM 1935 Core Oligosaccharide has been investigated by 1H and 13C NMR spectroscopy, MALDI-TOF MS and ESI MSn. It was concluded that the Core Oligosaccharide is a pentasaccharide with the following structure: ESI MS/MS analysis revealed that the glycine (a minor component) is linked to the →3,7)- l -α- d -Hepp-(1→ residue.
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The novel structure of the Core Oligosaccharide backbone of the lipopolysaccharide from the Plesiomonas shigelloides strain CNCTC 80/89 (serotype O13).
Carbohydrate research, 2013Co-Authors: Marta Kaszowska, Tomasz Niedziela, Wojciech Jachymek, Sabina Koj, Lennart Kenne, Czeslaw LugowskiAbstract:Abstract The new structure of the Core Oligosaccharide of Plesiomonas shigelloides CNCTC 80/89 (serotype O13) lipopolysaccharide has been investigated by chemical methods, 1H and 13C NMR spectroscopy and matrix-assisted laser-desorption/ionization time of flight (MALDI-TOF). It was concluded that the Core Oligosaccharide of P. shigelloides CNCTC 80/89 is a nonasaccharide with the following structure: Download : Download full-size image The position of glycine was determined by MALDI-TOF MS/MS analyses.
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Structures of two novel, serologically nonrelated Core Oligosaccharides of Yokenella regensburgei lipopolysaccharides differing only by a single hexose substitution
Glycobiology, 2009Co-Authors: Tomasz Niedziela, Anna Maciejewska, Jolanta Lukasiewicz, Wojciech Jachymek, Lennart Kenne, Rolf Andersson, Czeslaw LugowskiAbstract:Immunochemical analysis of the Yokenella regensburgei lipopolysaccharides (LPS) indicated the presence of the Core Oligosaccharide-related immunotypes among the investigated strains. The structure of the Core Oligosaccharide segment of the Y. regensburgei LPS has been investigated using chemical methods, mass spectrometry, and (1)H, (13)C NMR spectroscopy. It was concluded that the Core Oligosaccharides of the strains PCM 2476 and PCM 2477 are composed of an undecasaccharide. The combined data revealed two immunotypes of the Core Oligosaccharide recognized by antibodies against the whole bacterial cells. The structural differences between the Core Oligosaccharides are limited to the outermost terminal hexopyranose residue. In the Core Oligosaccharide of the strain PCM 2476, it was identified as alpha-d-Glcp and in that of the strain PCM 2477 as alpha-d-Galp. This subtle difference between the glycoforms of the LPS Core appeared to be essential for formation of the epitopes recognized by the specific antibodies directed against the Y. regensburgei whole bacterial cells. The Oligosaccharides are not substituted by phosphate groups. Instead, the carboxyl groups of Kdo and galacturonic acid residues present in the Core provide the negative charges. The undecasaccharides represent a novel Core type of bacterial LPS, which is characteristic for Y. regensburgei.
Wojciech Jachymek - One of the best experts on this subject based on the ideXlab platform.
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Core Oligosaccharide of escherichia coli b the structure required for bacteriophage t4 recognition
Carbohydrate Research, 2015Co-Authors: Marta Kaszowska, Tomasz Niedziela, Anna Maciejewska, Jolanta Lukasiewicz, Wojciech Jachymek, Czeslaw LugowskiAbstract:Abstract The structure of Escherichia coli B strain PCM 1935 Core Oligosaccharide has been investigated by 1H and 13C NMR spectroscopy, MALDI-TOF MS and ESI MSn. It was concluded that the Core Oligosaccharide is a pentasaccharide with the following structure: ESI MS/MS analysis revealed that the glycine (a minor component) is linked to the →3,7)- l -α- d -Hepp-(1→ residue.
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Core Oligosaccharide of Escherichia coli B—the structure required for bacteriophage T4 recognition
Carbohydrate Research, 2015Co-Authors: Marta Kaszowska, Tomasz Niedziela, Anna Maciejewska, Jolanta Lukasiewicz, Wojciech Jachymek, Czeslaw LugowskiAbstract:Abstract The structure of Escherichia coli B strain PCM 1935 Core Oligosaccharide has been investigated by 1H and 13C NMR spectroscopy, MALDI-TOF MS and ESI MSn. It was concluded that the Core Oligosaccharide is a pentasaccharide with the following structure: ESI MS/MS analysis revealed that the glycine (a minor component) is linked to the →3,7)- l -α- d -Hepp-(1→ residue.
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The novel structure of the Core Oligosaccharide backbone of the lipopolysaccharide from the Plesiomonas shigelloides strain CNCTC 80/89 (serotype O13).
Carbohydrate research, 2013Co-Authors: Marta Kaszowska, Tomasz Niedziela, Wojciech Jachymek, Sabina Koj, Lennart Kenne, Czeslaw LugowskiAbstract:Abstract The new structure of the Core Oligosaccharide of Plesiomonas shigelloides CNCTC 80/89 (serotype O13) lipopolysaccharide has been investigated by chemical methods, 1H and 13C NMR spectroscopy and matrix-assisted laser-desorption/ionization time of flight (MALDI-TOF). It was concluded that the Core Oligosaccharide of P. shigelloides CNCTC 80/89 is a nonasaccharide with the following structure: Download : Download full-size image The position of glycine was determined by MALDI-TOF MS/MS analyses.
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Core Oligosaccharide of Plesiomonas shigelloides PCM 2231 (Serotype O17) Lipopolysaccharide — Structural and Serological Analysis
Marine Drugs, 2013Co-Authors: Anna Maciejewska, Marta Kaszowska, Jolanta Lukasiewicz, Wojciech Jachymek, Aleksandra Man-kupisinska, Czeslaw LugowskiAbstract:The herein presented complete structure of the Core Oligosaccharide of lipopolysaccharide (LPS) P. shigelloides Polish Collection of Microorganisms (PCM) 2231 (serotype O17) was investigated by 1H, 13C NMR spectroscopy, mass spectrometry, chemical analyses and serological methods. The Core Oligosaccharide is composed of an undecasaccharide, which represents the second Core type identified for P. shigelloides serotype O17 LPS. This structure is similar to that of the Core Oligosaccharide of P. shigelloides strains 302-73 (serotype O1) and 7-63 (serotype O17) and differs from these only by one sugar residue. Serological screening of 55 strains of P. shigelloides with the use of serum against identified Core Oligosaccharide conjugated with bovine serum albumin (BSA) indicated the presence of similar structures in the LPS Core region of 28 O-serotypes. This observation suggests that the Core Oligosaccharide structure present in strain PCM 2231 could be the most common type among P. shigelloides lipopolysaccharides
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Core Oligosaccharide of plesiomonas shigelloides pcm 2231 serotype o17 lipopolysaccharide structural and serological analysis
Marine Drugs, 2013Co-Authors: Anna Maciejewska, Marta Kaszowska, Jolanta Lukasiewicz, Wojciech Jachymek, Aleksandra Mankupisinska, Czeslaw LugowskiAbstract:The herein presented complete structure of the Core Oligosaccharide of lipopolysaccharide (LPS) P. shigelloides Polish Collection of Microorganisms (PCM) 2231 (serotype O17) was investigated by 1H, 13C NMR spectroscopy, mass spectrometry, chemical analyses and serological methods. The Core Oligosaccharide is composed of an undecasaccharide, which represents the second Core type identified for P. shigelloides serotype O17 LPS. This structure is similar to that of the Core Oligosaccharide of P. shigelloides strains 302-73 (serotype O1) and 7-63 (serotype O17) and differs from these only by one sugar residue. Serological screening of 55 strains of P. shigelloides with the use of serum against identified Core Oligosaccharide conjugated with bovine serum albumin (BSA) indicated the presence of similar structures in the LPS Core region of 28 O-serotypes. This observation suggests that the Core Oligosaccharide structure present in strain PCM 2231 could be the most common type among P. shigelloides lipopolysaccharides.
Lennart Kenne - One of the best experts on this subject based on the ideXlab platform.
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The novel structure of the Core Oligosaccharide backbone of the lipopolysaccharide from the Plesiomonas shigelloides strain CNCTC 80/89 (serotype O13).
Carbohydrate research, 2013Co-Authors: Marta Kaszowska, Tomasz Niedziela, Wojciech Jachymek, Sabina Koj, Lennart Kenne, Czeslaw LugowskiAbstract:Abstract The new structure of the Core Oligosaccharide of Plesiomonas shigelloides CNCTC 80/89 (serotype O13) lipopolysaccharide has been investigated by chemical methods, 1H and 13C NMR spectroscopy and matrix-assisted laser-desorption/ionization time of flight (MALDI-TOF). It was concluded that the Core Oligosaccharide of P. shigelloides CNCTC 80/89 is a nonasaccharide with the following structure: Download : Download full-size image The position of glycine was determined by MALDI-TOF MS/MS analyses.
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Structures of two novel, serologically nonrelated Core Oligosaccharides of Yokenella regensburgei lipopolysaccharides differing only by a single hexose substitution
Glycobiology, 2009Co-Authors: Tomasz Niedziela, Anna Maciejewska, Jolanta Lukasiewicz, Wojciech Jachymek, Lennart Kenne, Rolf Andersson, Czeslaw LugowskiAbstract:Immunochemical analysis of the Yokenella regensburgei lipopolysaccharides (LPS) indicated the presence of the Core Oligosaccharide-related immunotypes among the investigated strains. The structure of the Core Oligosaccharide segment of the Y. regensburgei LPS has been investigated using chemical methods, mass spectrometry, and (1)H, (13)C NMR spectroscopy. It was concluded that the Core Oligosaccharides of the strains PCM 2476 and PCM 2477 are composed of an undecasaccharide. The combined data revealed two immunotypes of the Core Oligosaccharide recognized by antibodies against the whole bacterial cells. The structural differences between the Core Oligosaccharides are limited to the outermost terminal hexopyranose residue. In the Core Oligosaccharide of the strain PCM 2476, it was identified as alpha-d-Glcp and in that of the strain PCM 2477 as alpha-d-Galp. This subtle difference between the glycoforms of the LPS Core appeared to be essential for formation of the epitopes recognized by the specific antibodies directed against the Y. regensburgei whole bacterial cells. The Oligosaccharides are not substituted by phosphate groups. Instead, the carboxyl groups of Kdo and galacturonic acid residues present in the Core provide the negative charges. The undecasaccharides represent a novel Core type of bacterial LPS, which is characteristic for Y. regensburgei.
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Core Oligosaccharides of plesiomonas shigelloides o54 h2 strain cnctc 113 92 structural and serological analysis of the lipopolysaccharide Core region the o antigen biological repeating unit and the linkage between them
Journal of Biological Chemistry, 2002Co-Authors: Tomasz Niedziela, Jolanta Lukasiewicz, Wojciech Jachymek, Czeslaw Lugowski, Monika Dzieciatkowska, Lennart KenneAbstract:Abstract The structure of the Core Oligosaccharide moiety of the lipopolysaccharide (LPS) ofPlesiomonas shigelloides O54 (strain CNCTC 113/92) has been investigated by 1H and 13C NMR, fast atom bombardment mass spectrometry (MS)/MS, matrix-assisted laser-desorption/ionization time-of-flight MS, monosaccharide and methylation analysis, and immunological methods. It was concluded that the main Core Oligosaccharide of this strain is composed of a decasaccharide with the following structure: in which l-α-d-Hepp isl-glycero-α-d-manno-heptopyranose. The nonasaccharide variant of the Core Oligosaccharide (∼10%), devoid of β-d-Glcp substituting the α-d-GlcpN at C-6, was also identified. The Core Oligosaccharide substituted at C-4 of the outer Core β-d-Glcp residue with the singleO-polysaccharide repeating unit was also isolated yielding a hexadecasaccharide structure. The determination of the monosaccharides involved in the linkage between theO-specific polysaccharide part and the Core, as well as the presence of →3)-d-β-d-Hepp-(1→ instead of →3,4)-d-β-d-Hepp-(1→ in the repeating unit, revealed the structure of the biological repeating unit of the O-antigen. The Core Oligosaccharides are not substituted by phosphate residues and represent novel Core type of bacterial LPS that is characteristic for the Plesiomonas shigelloides serotype O54. Serological screening of 69 differentO-serotypes of P. shigelloides suggests that epitopes similar to the Core Oligosaccharide of serotype O54 (strain CNCTC 113/92) might also be present in the Core region of the serotypes O24 (strain CNCTC 92/89), O37 (strain CNCTC 39/89) and O96 (strain CNCTC 5133) LPS.
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Core Oligosaccharides of Plesiomonas shigelloides O54:H2 (strain CNCTC 113/92): structural and serological analysis of the lipopolysaccharide Core region, the O-antigen biological repeating unit, and the linkage between them.
The Journal of biological chemistry, 2002Co-Authors: Tomasz Niedziela, Jolanta Lukasiewicz, Wojciech Jachymek, Czeslaw Lugowski, Monika Dzieciatkowska, Lennart KenneAbstract:Abstract The structure of the Core Oligosaccharide moiety of the lipopolysaccharide (LPS) ofPlesiomonas shigelloides O54 (strain CNCTC 113/92) has been investigated by 1H and 13C NMR, fast atom bombardment mass spectrometry (MS)/MS, matrix-assisted laser-desorption/ionization time-of-flight MS, monosaccharide and methylation analysis, and immunological methods. It was concluded that the main Core Oligosaccharide of this strain is composed of a decasaccharide with the following structure: in which l-α-d-Hepp isl-glycero-α-d-manno-heptopyranose. The nonasaccharide variant of the Core Oligosaccharide (∼10%), devoid of β-d-Glcp substituting the α-d-GlcpN at C-6, was also identified. The Core Oligosaccharide substituted at C-4 of the outer Core β-d-Glcp residue with the singleO-polysaccharide repeating unit was also isolated yielding a hexadecasaccharide structure. The determination of the monosaccharides involved in the linkage between theO-specific polysaccharide part and the Core, as well as the presence of →3)-d-β-d-Hepp-(1→ instead of →3,4)-d-β-d-Hepp-(1→ in the repeating unit, revealed the structure of the biological repeating unit of the O-antigen. The Core Oligosaccharides are not substituted by phosphate residues and represent novel Core type of bacterial LPS that is characteristic for the Plesiomonas shigelloides serotype O54. Serological screening of 69 differentO-serotypes of P. shigelloides suggests that epitopes similar to the Core Oligosaccharide of serotype O54 (strain CNCTC 113/92) might also be present in the Core region of the serotypes O24 (strain CNCTC 92/89), O37 (strain CNCTC 39/89) and O96 (strain CNCTC 5133) LPS.