The Experts below are selected from a list of 27087 Experts worldwide ranked by ideXlab platform

Svetlana Nikolic - One of the best experts on this subject based on the ideXlab platform.

  • ultrasound assisted production of bioethanol by simultaneous saccharification and fermentation of Corn Meal
    Food Chemistry, 2010
    Co-Authors: Svetlana Nikolic, Ljiljana Mojovic, Marica Rakin, Dusanka Pejin, Jelena Pejin
    Abstract:

    Abstract An ultrasound-assisted liquefaction as a pretreatment for bioethanol production by simultaneous saccharification and fermentation (SSF) of Corn Meal using Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. Ultrasound pretreatment (at a frequency of 40 kHz) was performed at different sonication times and temperatures, before addition of liquefying enzyme. An optimal duration of the treatment of 5 min and sonication temperature of 60 °C were selected, taking into account glucose concentration after the liquefaction step. Under the optimum conditions an increase of glucose concentration of 6.82% over untreated control sample was achieved. Furthermore, the SSF process kinetics was assessed and determined, and the effect of ultrasound pretreatment on an increase of ethanol productivity was investigated. The obtained results indicated that the ultrasound pretreatment could increase the ethanol concentration by 11.15% (compared to the control sample) as well as other significant process parameters. In this case, the maximum ethanol concentration of 9.67% w/w (which corresponded to percentage of the theoretical ethanol yield of 88.96%) was achieved after 32 h of the SSF process. A comparison of scanning electron micrographs of the ultrasound-pretreated and untreated samples of Corn Meal suspensions showed that the ultrasound stimulated degradation of starch granules and release of glucose, and thereby accelerated the starch hydrolysis due to the cavitation and acoustic streaming caused by the ultrasonic action.

  • bioethanol production from Corn Meal by simultaneous enzymatic saccharification and fermentation with immobilized cells of saccharomyces cerevisiae var ellipsoideus
    Fuel, 2009
    Co-Authors: Svetlana Nikolic, Ljiljana Mojovic, Marica Rakin, Dusanka Pejin
    Abstract:

    The simultaneous enzymatic saccharification and fermentation (SSF) of Corn Meal using immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. The yeast cells were immobilized in Ca-alginate by electrostatic droplet generation method. The process kinetics was assessed and determined and the effect of addition of various yeast activators (mineral salts: ZnSO4 7H2O and MgSO4 7H2O, and vitamins: Ca-pantothenate, biotin and myo-inositol) separately or mixed, was investigated. Taking into account high values of process parameters (such as ethanol concentration, ethanol yield, percentage of the theoretical ethanol yield, volumetric productivity and utilized glucose) and significant energy savings the SSF process was found to be superior compared to the SHF process. Further improvement in ethanol production was accomplished with the addition of mineral salts as yeast activators which contributed to the highest increase in ethanol production. In this case, the ethanol concentration of 10.23% (w/w), percentage of the theoretical ethanol yield of 98.08%, the ethanol yield of 0.55 g/g and the volumetric productivity of 2.13 g/lh were obtained.

  • effect of different fermentation parameters on bioethanol production from Corn Meal hydrolyzates by free and immobilized cells of saccharomyces cerevisiae var ellipsoideus
    Journal of Chemical Technology & Biotechnology, 2009
    Co-Authors: Svetlana Nikolic, Ljiljana Mojovic, Marica Rakin, Dusanka Pejin, Viktor Nedovic
    Abstract:

    BACKGROUND: Bioethanol produced from renewable biomass, such as Corn Meal, is a biofuel that is both renewable and environmentally friendly. Significant scientific and technological investments will be needed to achieve substitution of conventional fossil fuels with alternative fuels. The ethanol fermentation of enzymatically obtained Corn Meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. The initial glucose and inoculum concentration and the time required for the efficient ethanol production were optimized taking into account parameters such as ethanol concentration, ethanol yield, percentage of the theoretical yield of ethanol and volumetric productivity in both immobilized and free cell systems. RESULTS: The yeast cells were immobilized in Ca–alginate by an electrostatic droplet generation method. An optimal initial inoculum concentration of 2% (v/v) and optimal fermentation time of 38 h for both immobilized and free yeasts were determined. An optimal initial glucose concentration of 150 g L−1 for free system was achieved. At the initial glucose concentration of 176 g L no substrate or product inhibition were achieved with immobilized yeast. CONCLUSION: By immobilization of the yeast into Ca–alginate using the method of electrostatic droplet generation a superior system was realized, which exhibited lower substrate inhibition and higher tolerance to ethanol. The cells of S. cerevisiae var. ellipsoideus yeast entrapped in Ca–alginate showed good physical and chemical stability, and no substrate and product diffusion restrictions were noticed. Copyright © 2008 Society of Chemical Industry

  • production of bioethanol from Corn Meal hydrolyzates
    Fuel, 2006
    Co-Authors: Ljiljana Mojovic, Svetlana Nikolic, Marica Rakin, Maja Vukasinovic
    Abstract:

    The two-step enzymatic hydrolysis of Corn Meal by commercially available α-amylase and glucoamylase and further ethanol fermentation of the obtained hydrolyzates by Saccharomyces cerevisiae yeast was studied. The conditions of starch hydrolysis such as substrate and enzyme concentration and the time required for enzymatic action were optimized taking into account both the effects of hydrolysis and ethanol fermentation. The Corn Meal hydrolyzates obtained were good substrates for ethanol fermentation by S. cerevisiae. The yield of ethanol of more than 80% (w/w) of the theoretical was achieved with a satisfactory volumetric productivity P (g/l h). No shortage of fermentable sugars was observed during simultaneous hydrolysis and fermentation. In this process, the savings in energy by carrying out the saccharification step at lower temperature (32 °C) could be realized, as well as a reduction of the process time for 4 h.

Ljiljana Mojovic - One of the best experts on this subject based on the ideXlab platform.

  • ultrasound assisted production of bioethanol by simultaneous saccharification and fermentation of Corn Meal
    Food Chemistry, 2010
    Co-Authors: Svetlana Nikolic, Ljiljana Mojovic, Marica Rakin, Dusanka Pejin, Jelena Pejin
    Abstract:

    Abstract An ultrasound-assisted liquefaction as a pretreatment for bioethanol production by simultaneous saccharification and fermentation (SSF) of Corn Meal using Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. Ultrasound pretreatment (at a frequency of 40 kHz) was performed at different sonication times and temperatures, before addition of liquefying enzyme. An optimal duration of the treatment of 5 min and sonication temperature of 60 °C were selected, taking into account glucose concentration after the liquefaction step. Under the optimum conditions an increase of glucose concentration of 6.82% over untreated control sample was achieved. Furthermore, the SSF process kinetics was assessed and determined, and the effect of ultrasound pretreatment on an increase of ethanol productivity was investigated. The obtained results indicated that the ultrasound pretreatment could increase the ethanol concentration by 11.15% (compared to the control sample) as well as other significant process parameters. In this case, the maximum ethanol concentration of 9.67% w/w (which corresponded to percentage of the theoretical ethanol yield of 88.96%) was achieved after 32 h of the SSF process. A comparison of scanning electron micrographs of the ultrasound-pretreated and untreated samples of Corn Meal suspensions showed that the ultrasound stimulated degradation of starch granules and release of glucose, and thereby accelerated the starch hydrolysis due to the cavitation and acoustic streaming caused by the ultrasonic action.

  • bioethanol production from Corn Meal by simultaneous enzymatic saccharification and fermentation with immobilized cells of saccharomyces cerevisiae var ellipsoideus
    Fuel, 2009
    Co-Authors: Svetlana Nikolic, Ljiljana Mojovic, Marica Rakin, Dusanka Pejin
    Abstract:

    The simultaneous enzymatic saccharification and fermentation (SSF) of Corn Meal using immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. The yeast cells were immobilized in Ca-alginate by electrostatic droplet generation method. The process kinetics was assessed and determined and the effect of addition of various yeast activators (mineral salts: ZnSO4 7H2O and MgSO4 7H2O, and vitamins: Ca-pantothenate, biotin and myo-inositol) separately or mixed, was investigated. Taking into account high values of process parameters (such as ethanol concentration, ethanol yield, percentage of the theoretical ethanol yield, volumetric productivity and utilized glucose) and significant energy savings the SSF process was found to be superior compared to the SHF process. Further improvement in ethanol production was accomplished with the addition of mineral salts as yeast activators which contributed to the highest increase in ethanol production. In this case, the ethanol concentration of 10.23% (w/w), percentage of the theoretical ethanol yield of 98.08%, the ethanol yield of 0.55 g/g and the volumetric productivity of 2.13 g/lh were obtained.

  • effect of different fermentation parameters on bioethanol production from Corn Meal hydrolyzates by free and immobilized cells of saccharomyces cerevisiae var ellipsoideus
    Journal of Chemical Technology & Biotechnology, 2009
    Co-Authors: Svetlana Nikolic, Ljiljana Mojovic, Marica Rakin, Dusanka Pejin, Viktor Nedovic
    Abstract:

    BACKGROUND: Bioethanol produced from renewable biomass, such as Corn Meal, is a biofuel that is both renewable and environmentally friendly. Significant scientific and technological investments will be needed to achieve substitution of conventional fossil fuels with alternative fuels. The ethanol fermentation of enzymatically obtained Corn Meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. The initial glucose and inoculum concentration and the time required for the efficient ethanol production were optimized taking into account parameters such as ethanol concentration, ethanol yield, percentage of the theoretical yield of ethanol and volumetric productivity in both immobilized and free cell systems. RESULTS: The yeast cells were immobilized in Ca–alginate by an electrostatic droplet generation method. An optimal initial inoculum concentration of 2% (v/v) and optimal fermentation time of 38 h for both immobilized and free yeasts were determined. An optimal initial glucose concentration of 150 g L−1 for free system was achieved. At the initial glucose concentration of 176 g L no substrate or product inhibition were achieved with immobilized yeast. CONCLUSION: By immobilization of the yeast into Ca–alginate using the method of electrostatic droplet generation a superior system was realized, which exhibited lower substrate inhibition and higher tolerance to ethanol. The cells of S. cerevisiae var. ellipsoideus yeast entrapped in Ca–alginate showed good physical and chemical stability, and no substrate and product diffusion restrictions were noticed. Copyright © 2008 Society of Chemical Industry

  • production of bioethanol from Corn Meal hydrolyzates
    Fuel, 2006
    Co-Authors: Ljiljana Mojovic, Svetlana Nikolic, Marica Rakin, Maja Vukasinovic
    Abstract:

    The two-step enzymatic hydrolysis of Corn Meal by commercially available α-amylase and glucoamylase and further ethanol fermentation of the obtained hydrolyzates by Saccharomyces cerevisiae yeast was studied. The conditions of starch hydrolysis such as substrate and enzyme concentration and the time required for enzymatic action were optimized taking into account both the effects of hydrolysis and ethanol fermentation. The Corn Meal hydrolyzates obtained were good substrates for ethanol fermentation by S. cerevisiae. The yield of ethanol of more than 80% (w/w) of the theoretical was achieved with a satisfactory volumetric productivity P (g/l h). No shortage of fermentable sugars was observed during simultaneous hydrolysis and fermentation. In this process, the savings in energy by carrying out the saccharification step at lower temperature (32 °C) could be realized, as well as a reduction of the process time for 4 h.

Marica Rakin - One of the best experts on this subject based on the ideXlab platform.

  • ultrasound assisted production of bioethanol by simultaneous saccharification and fermentation of Corn Meal
    Food Chemistry, 2010
    Co-Authors: Svetlana Nikolic, Ljiljana Mojovic, Marica Rakin, Dusanka Pejin, Jelena Pejin
    Abstract:

    Abstract An ultrasound-assisted liquefaction as a pretreatment for bioethanol production by simultaneous saccharification and fermentation (SSF) of Corn Meal using Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. Ultrasound pretreatment (at a frequency of 40 kHz) was performed at different sonication times and temperatures, before addition of liquefying enzyme. An optimal duration of the treatment of 5 min and sonication temperature of 60 °C were selected, taking into account glucose concentration after the liquefaction step. Under the optimum conditions an increase of glucose concentration of 6.82% over untreated control sample was achieved. Furthermore, the SSF process kinetics was assessed and determined, and the effect of ultrasound pretreatment on an increase of ethanol productivity was investigated. The obtained results indicated that the ultrasound pretreatment could increase the ethanol concentration by 11.15% (compared to the control sample) as well as other significant process parameters. In this case, the maximum ethanol concentration of 9.67% w/w (which corresponded to percentage of the theoretical ethanol yield of 88.96%) was achieved after 32 h of the SSF process. A comparison of scanning electron micrographs of the ultrasound-pretreated and untreated samples of Corn Meal suspensions showed that the ultrasound stimulated degradation of starch granules and release of glucose, and thereby accelerated the starch hydrolysis due to the cavitation and acoustic streaming caused by the ultrasonic action.

  • bioethanol production from Corn Meal by simultaneous enzymatic saccharification and fermentation with immobilized cells of saccharomyces cerevisiae var ellipsoideus
    Fuel, 2009
    Co-Authors: Svetlana Nikolic, Ljiljana Mojovic, Marica Rakin, Dusanka Pejin
    Abstract:

    The simultaneous enzymatic saccharification and fermentation (SSF) of Corn Meal using immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. The yeast cells were immobilized in Ca-alginate by electrostatic droplet generation method. The process kinetics was assessed and determined and the effect of addition of various yeast activators (mineral salts: ZnSO4 7H2O and MgSO4 7H2O, and vitamins: Ca-pantothenate, biotin and myo-inositol) separately or mixed, was investigated. Taking into account high values of process parameters (such as ethanol concentration, ethanol yield, percentage of the theoretical ethanol yield, volumetric productivity and utilized glucose) and significant energy savings the SSF process was found to be superior compared to the SHF process. Further improvement in ethanol production was accomplished with the addition of mineral salts as yeast activators which contributed to the highest increase in ethanol production. In this case, the ethanol concentration of 10.23% (w/w), percentage of the theoretical ethanol yield of 98.08%, the ethanol yield of 0.55 g/g and the volumetric productivity of 2.13 g/lh were obtained.

  • effect of different fermentation parameters on bioethanol production from Corn Meal hydrolyzates by free and immobilized cells of saccharomyces cerevisiae var ellipsoideus
    Journal of Chemical Technology & Biotechnology, 2009
    Co-Authors: Svetlana Nikolic, Ljiljana Mojovic, Marica Rakin, Dusanka Pejin, Viktor Nedovic
    Abstract:

    BACKGROUND: Bioethanol produced from renewable biomass, such as Corn Meal, is a biofuel that is both renewable and environmentally friendly. Significant scientific and technological investments will be needed to achieve substitution of conventional fossil fuels with alternative fuels. The ethanol fermentation of enzymatically obtained Corn Meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. The initial glucose and inoculum concentration and the time required for the efficient ethanol production were optimized taking into account parameters such as ethanol concentration, ethanol yield, percentage of the theoretical yield of ethanol and volumetric productivity in both immobilized and free cell systems. RESULTS: The yeast cells were immobilized in Ca–alginate by an electrostatic droplet generation method. An optimal initial inoculum concentration of 2% (v/v) and optimal fermentation time of 38 h for both immobilized and free yeasts were determined. An optimal initial glucose concentration of 150 g L−1 for free system was achieved. At the initial glucose concentration of 176 g L no substrate or product inhibition were achieved with immobilized yeast. CONCLUSION: By immobilization of the yeast into Ca–alginate using the method of electrostatic droplet generation a superior system was realized, which exhibited lower substrate inhibition and higher tolerance to ethanol. The cells of S. cerevisiae var. ellipsoideus yeast entrapped in Ca–alginate showed good physical and chemical stability, and no substrate and product diffusion restrictions were noticed. Copyright © 2008 Society of Chemical Industry

  • production of bioethanol from Corn Meal hydrolyzates
    Fuel, 2006
    Co-Authors: Ljiljana Mojovic, Svetlana Nikolic, Marica Rakin, Maja Vukasinovic
    Abstract:

    The two-step enzymatic hydrolysis of Corn Meal by commercially available α-amylase and glucoamylase and further ethanol fermentation of the obtained hydrolyzates by Saccharomyces cerevisiae yeast was studied. The conditions of starch hydrolysis such as substrate and enzyme concentration and the time required for enzymatic action were optimized taking into account both the effects of hydrolysis and ethanol fermentation. The Corn Meal hydrolyzates obtained were good substrates for ethanol fermentation by S. cerevisiae. The yield of ethanol of more than 80% (w/w) of the theoretical was achieved with a satisfactory volumetric productivity P (g/l h). No shortage of fermentable sugars was observed during simultaneous hydrolysis and fermentation. In this process, the savings in energy by carrying out the saccharification step at lower temperature (32 °C) could be realized, as well as a reduction of the process time for 4 h.

Dusanka Pejin - One of the best experts on this subject based on the ideXlab platform.

  • ultrasound assisted production of bioethanol by simultaneous saccharification and fermentation of Corn Meal
    Food Chemistry, 2010
    Co-Authors: Svetlana Nikolic, Ljiljana Mojovic, Marica Rakin, Dusanka Pejin, Jelena Pejin
    Abstract:

    Abstract An ultrasound-assisted liquefaction as a pretreatment for bioethanol production by simultaneous saccharification and fermentation (SSF) of Corn Meal using Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. Ultrasound pretreatment (at a frequency of 40 kHz) was performed at different sonication times and temperatures, before addition of liquefying enzyme. An optimal duration of the treatment of 5 min and sonication temperature of 60 °C were selected, taking into account glucose concentration after the liquefaction step. Under the optimum conditions an increase of glucose concentration of 6.82% over untreated control sample was achieved. Furthermore, the SSF process kinetics was assessed and determined, and the effect of ultrasound pretreatment on an increase of ethanol productivity was investigated. The obtained results indicated that the ultrasound pretreatment could increase the ethanol concentration by 11.15% (compared to the control sample) as well as other significant process parameters. In this case, the maximum ethanol concentration of 9.67% w/w (which corresponded to percentage of the theoretical ethanol yield of 88.96%) was achieved after 32 h of the SSF process. A comparison of scanning electron micrographs of the ultrasound-pretreated and untreated samples of Corn Meal suspensions showed that the ultrasound stimulated degradation of starch granules and release of glucose, and thereby accelerated the starch hydrolysis due to the cavitation and acoustic streaming caused by the ultrasonic action.

  • bioethanol production from Corn Meal by simultaneous enzymatic saccharification and fermentation with immobilized cells of saccharomyces cerevisiae var ellipsoideus
    Fuel, 2009
    Co-Authors: Svetlana Nikolic, Ljiljana Mojovic, Marica Rakin, Dusanka Pejin
    Abstract:

    The simultaneous enzymatic saccharification and fermentation (SSF) of Corn Meal using immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. The yeast cells were immobilized in Ca-alginate by electrostatic droplet generation method. The process kinetics was assessed and determined and the effect of addition of various yeast activators (mineral salts: ZnSO4 7H2O and MgSO4 7H2O, and vitamins: Ca-pantothenate, biotin and myo-inositol) separately or mixed, was investigated. Taking into account high values of process parameters (such as ethanol concentration, ethanol yield, percentage of the theoretical ethanol yield, volumetric productivity and utilized glucose) and significant energy savings the SSF process was found to be superior compared to the SHF process. Further improvement in ethanol production was accomplished with the addition of mineral salts as yeast activators which contributed to the highest increase in ethanol production. In this case, the ethanol concentration of 10.23% (w/w), percentage of the theoretical ethanol yield of 98.08%, the ethanol yield of 0.55 g/g and the volumetric productivity of 2.13 g/lh were obtained.

  • effect of different fermentation parameters on bioethanol production from Corn Meal hydrolyzates by free and immobilized cells of saccharomyces cerevisiae var ellipsoideus
    Journal of Chemical Technology & Biotechnology, 2009
    Co-Authors: Svetlana Nikolic, Ljiljana Mojovic, Marica Rakin, Dusanka Pejin, Viktor Nedovic
    Abstract:

    BACKGROUND: Bioethanol produced from renewable biomass, such as Corn Meal, is a biofuel that is both renewable and environmentally friendly. Significant scientific and technological investments will be needed to achieve substitution of conventional fossil fuels with alternative fuels. The ethanol fermentation of enzymatically obtained Corn Meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. The initial glucose and inoculum concentration and the time required for the efficient ethanol production were optimized taking into account parameters such as ethanol concentration, ethanol yield, percentage of the theoretical yield of ethanol and volumetric productivity in both immobilized and free cell systems. RESULTS: The yeast cells were immobilized in Ca–alginate by an electrostatic droplet generation method. An optimal initial inoculum concentration of 2% (v/v) and optimal fermentation time of 38 h for both immobilized and free yeasts were determined. An optimal initial glucose concentration of 150 g L−1 for free system was achieved. At the initial glucose concentration of 176 g L no substrate or product inhibition were achieved with immobilized yeast. CONCLUSION: By immobilization of the yeast into Ca–alginate using the method of electrostatic droplet generation a superior system was realized, which exhibited lower substrate inhibition and higher tolerance to ethanol. The cells of S. cerevisiae var. ellipsoideus yeast entrapped in Ca–alginate showed good physical and chemical stability, and no substrate and product diffusion restrictions were noticed. Copyright © 2008 Society of Chemical Industry

Aldo Laganà - One of the best experts on this subject based on the ideXlab platform.

  • development of a multiresidue method for analysis of major fusarium mycotoxins in Corn Meal using liquid chromatography tandem mass spectrometry
    Rapid Communications in Mass Spectrometry, 2005
    Co-Authors: C. Cavaliere, P. Foglia, E. Pastorini, R. Samperi, Aldo Laganà
    Abstract:

    A sensitive and reliable liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) method has been developed to determine, in a single run, eight trichothecenes, three fumonisins, zearalenone and α-zearalenol, in Corn Meal samples. LC and MS conditions were varied to find the best compromise in terms of sensitivity and separation. An acceptable compromise was obtained using a C 1 8 column thermostatted at 45°C and a mobile phase gradient of methanol/water with 10 mmol/L formate buffer (pH 3.8). A multiple reaction monitoring program, in which fumonisins and trichothecenes (except nivalenol and deoxynivalenol) are acquired in positive ESI as [M+H] + or [M+NH 4 ] + , and all other compounds in negative ESI, was developed to match appropriate retention time windows. Sample preparation used a simple homogenization of the Corn Meal sample with acetonitrile/water (75:25, v/v) followed by extraction on a C 1 8 cartridge and clean-up on a cartridge containing graphitized carbon black. Method detection limits were in the range 2-14 ng/g, with the exception of nivalenol (27ng/g), deoxynivalenol (40 ng/g) and 15-acetyldeoxynivalenol (30 ng/g). Good accuracy (recoveries 81-104%) and precision (RSD 4-11%) were obtained by performing calibration using a spiked analyte-free extract.

  • Development of a multiresidue method for analysis of major Fusarium mycotoxins in Corn Meal using liquid chromatography – tandem mass spectrometry
    'Wiley', 2005
    Co-Authors: C. Cavaliere, P. Foglia, E. Pastorini, R. Samperi, Aldo Laganà
    Abstract:

    A sensitive and reliable liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) method has been developed to determine, in a single run, eight trichothecenes, three fumonisins, zearalenone and alpha-zearalenol, in Corn Meal samples. LC and MS conditions were varied to find the best compromise in terms of sensitivity and separation. An acceptable compromise was obtained using a C-18 column thermostatted at 45 degrees C and a mobile phase gradient of methanol/water with 10 mmol/L formate buffer (pH 3.8). A multiple reaction monitoring program, in which fumonisins and trichothecenes (except nivalenol and deoxynivalenol) are acquired in positive ESI as [M+H](+) or [M+NH4](+), and all other compounds in negative ESI, was developed to match appropriate retention time windows. Sample preparation used a simple homogenization of the Corn Meal sample with acetonitrile/water (75:25, v/v) followed by extraction on a C-18 cartridge and clean-up on a cartridge containing graphitized carbon black. Method detection limits were in the range 2-14 ng/g, with the exception of nivalenol (27 ng/g), deoxynivalenol (40 ng/g) and 15-acetyldeoxynivalenol (30 ng/g). Good accuracy (recoveries 81-104%) and precision (RSD 4-11%) were obtained by performing calibration using a spiked analyte-free extract