The Experts below are selected from a list of 5637 Experts worldwide ranked by ideXlab platform
Shiou-hwa Jee - One of the best experts on this subject based on the ideXlab platform.
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Multiphoton autofluorescence and second-harmonic generation imaging of the ex vivo porcine eye.
Investigative ophthalmology & visual science, 2006Co-Authors: Shu-wen Teng, Ju-li Peng, Huei-hsing Lin, Yen Sun, Wei-chou Lin, Sung-jan Lin, Hsin-yuan Tan, Ki Hean Kim, Shiou-hwa JeeAbstract:PURPOSE. The purpose of this work was to demonstrate the use of the combined imaging modality of multiphoton autofluorescence and second-harmonic generation (SHG) microscopy in obtaining spectrally resolved morphologic features of the Cornea, limbus, conjunctiva, and sclera in whole, ex vivo porcine eyes. METHODS. The 780-nm output of a femtosecond, titanium-sapphire laser was used to induce broadband autofluorescence (435-700 nm) and SHG (390 nm) from various regions of the surface of ex vivo porcine eyes. A water-immersion objective was used for convenient imaging of the curved surface of the eye. RESULTS. Multiphoton autofluorescence was useful in identifying cellular structures of the different domains of the ocular surface, and the SHG signal can be used to resolve collagen organization within the Cornea Stroma and sclera of ex vivo porcine eyes. CONCLUSIONS. Multiphoton autofluorescence and SHG microscopy have been demonstrated to be an effective technique for resolving, respectively, the cellular and collagen structures within the ocular surface of ex vivo porcine eyes. SHG imaging resolved the difference in structural orientations between Corneal and sclera collagen fibers. Specifically, the Corneal collagen is organized in a depth-dependent fashion, whereas the scleral collagen is randomly packed. Because this technique does not require histologic preparation procedures, it has the potential to be applied for in vivo studies with minimal disturbance to the eye.
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characterizing the thermally induced structural changes to intact porcine eye part 1 second harmonic generation imaging of Cornea Stroma
Journal of Biomedical Optics, 2005Co-Authors: Hsin-yuan Tan, Shu-wen Teng, Wei-chou Lin, Sung-jan Lin, Shiou-hwa Jee, Chenyuan DongAbstract:We characterize the structural changes of porcine Corneal structures from 25 to 90°C using second harmonic generation (SHG) microscopy. Our results show that porcine Stroma undergoes several distinct stages of structural changes between 25 and 90°C. A decrease in SHG intensity from 30 to 45°C and the existence of SHG intensity peaks at 53, 65, and 77°C correlate to distinct structural alterations of the Corneal Stroma. At higher temperatures, the SHG intensity decreases and a baseline in SHG signal is reached at 90°C. Our results demonstrate that SHG microscopy is a useful technique for obtaining qualitative and quantitative information of thermally treated Corneal fibers without histological or labeling procedures. With additional developments, SHG imaging may be developed into an effective imaging technique for in vivo characterization of Cornea structural changes.
Hsin-yuan Tan - One of the best experts on this subject based on the ideXlab platform.
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Multiphoton autofluorescence and second-harmonic generation imaging of the ex vivo porcine eye.
Investigative ophthalmology & visual science, 2006Co-Authors: Shu-wen Teng, Ju-li Peng, Huei-hsing Lin, Yen Sun, Wei-chou Lin, Sung-jan Lin, Hsin-yuan Tan, Ki Hean Kim, Shiou-hwa JeeAbstract:PURPOSE. The purpose of this work was to demonstrate the use of the combined imaging modality of multiphoton autofluorescence and second-harmonic generation (SHG) microscopy in obtaining spectrally resolved morphologic features of the Cornea, limbus, conjunctiva, and sclera in whole, ex vivo porcine eyes. METHODS. The 780-nm output of a femtosecond, titanium-sapphire laser was used to induce broadband autofluorescence (435-700 nm) and SHG (390 nm) from various regions of the surface of ex vivo porcine eyes. A water-immersion objective was used for convenient imaging of the curved surface of the eye. RESULTS. Multiphoton autofluorescence was useful in identifying cellular structures of the different domains of the ocular surface, and the SHG signal can be used to resolve collagen organization within the Cornea Stroma and sclera of ex vivo porcine eyes. CONCLUSIONS. Multiphoton autofluorescence and SHG microscopy have been demonstrated to be an effective technique for resolving, respectively, the cellular and collagen structures within the ocular surface of ex vivo porcine eyes. SHG imaging resolved the difference in structural orientations between Corneal and sclera collagen fibers. Specifically, the Corneal collagen is organized in a depth-dependent fashion, whereas the scleral collagen is randomly packed. Because this technique does not require histologic preparation procedures, it has the potential to be applied for in vivo studies with minimal disturbance to the eye.
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characterizing the thermally induced structural changes to intact porcine eye part 1 second harmonic generation imaging of Cornea Stroma
Journal of Biomedical Optics, 2005Co-Authors: Hsin-yuan Tan, Shu-wen Teng, Wei-chou Lin, Sung-jan Lin, Shiou-hwa Jee, Chenyuan DongAbstract:We characterize the structural changes of porcine Corneal structures from 25 to 90°C using second harmonic generation (SHG) microscopy. Our results show that porcine Stroma undergoes several distinct stages of structural changes between 25 and 90°C. A decrease in SHG intensity from 30 to 45°C and the existence of SHG intensity peaks at 53, 65, and 77°C correlate to distinct structural alterations of the Corneal Stroma. At higher temperatures, the SHG intensity decreases and a baseline in SHG signal is reached at 90°C. Our results demonstrate that SHG microscopy is a useful technique for obtaining qualitative and quantitative information of thermally treated Corneal fibers without histological or labeling procedures. With additional developments, SHG imaging may be developed into an effective imaging technique for in vivo characterization of Cornea structural changes.
Shu-wen Teng - One of the best experts on this subject based on the ideXlab platform.
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Multiphoton autofluorescence and second-harmonic generation imaging of the ex vivo porcine eye.
Investigative ophthalmology & visual science, 2006Co-Authors: Shu-wen Teng, Ju-li Peng, Huei-hsing Lin, Yen Sun, Wei-chou Lin, Sung-jan Lin, Hsin-yuan Tan, Ki Hean Kim, Shiou-hwa JeeAbstract:PURPOSE. The purpose of this work was to demonstrate the use of the combined imaging modality of multiphoton autofluorescence and second-harmonic generation (SHG) microscopy in obtaining spectrally resolved morphologic features of the Cornea, limbus, conjunctiva, and sclera in whole, ex vivo porcine eyes. METHODS. The 780-nm output of a femtosecond, titanium-sapphire laser was used to induce broadband autofluorescence (435-700 nm) and SHG (390 nm) from various regions of the surface of ex vivo porcine eyes. A water-immersion objective was used for convenient imaging of the curved surface of the eye. RESULTS. Multiphoton autofluorescence was useful in identifying cellular structures of the different domains of the ocular surface, and the SHG signal can be used to resolve collagen organization within the Cornea Stroma and sclera of ex vivo porcine eyes. CONCLUSIONS. Multiphoton autofluorescence and SHG microscopy have been demonstrated to be an effective technique for resolving, respectively, the cellular and collagen structures within the ocular surface of ex vivo porcine eyes. SHG imaging resolved the difference in structural orientations between Corneal and sclera collagen fibers. Specifically, the Corneal collagen is organized in a depth-dependent fashion, whereas the scleral collagen is randomly packed. Because this technique does not require histologic preparation procedures, it has the potential to be applied for in vivo studies with minimal disturbance to the eye.
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characterizing the thermally induced structural changes to intact porcine eye part 1 second harmonic generation imaging of Cornea Stroma
Journal of Biomedical Optics, 2005Co-Authors: Hsin-yuan Tan, Shu-wen Teng, Wei-chou Lin, Sung-jan Lin, Shiou-hwa Jee, Chenyuan DongAbstract:We characterize the structural changes of porcine Corneal structures from 25 to 90°C using second harmonic generation (SHG) microscopy. Our results show that porcine Stroma undergoes several distinct stages of structural changes between 25 and 90°C. A decrease in SHG intensity from 30 to 45°C and the existence of SHG intensity peaks at 53, 65, and 77°C correlate to distinct structural alterations of the Corneal Stroma. At higher temperatures, the SHG intensity decreases and a baseline in SHG signal is reached at 90°C. Our results demonstrate that SHG microscopy is a useful technique for obtaining qualitative and quantitative information of thermally treated Corneal fibers without histological or labeling procedures. With additional developments, SHG imaging may be developed into an effective imaging technique for in vivo characterization of Cornea structural changes.
Chenyuan Dong - One of the best experts on this subject based on the ideXlab platform.
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characterizing the thermally induced structural changes to intact porcine eye part 1 second harmonic generation imaging of Cornea Stroma
Journal of Biomedical Optics, 2005Co-Authors: Hsin-yuan Tan, Shu-wen Teng, Wei-chou Lin, Sung-jan Lin, Shiou-hwa Jee, Chenyuan DongAbstract:We characterize the structural changes of porcine Corneal structures from 25 to 90°C using second harmonic generation (SHG) microscopy. Our results show that porcine Stroma undergoes several distinct stages of structural changes between 25 and 90°C. A decrease in SHG intensity from 30 to 45°C and the existence of SHG intensity peaks at 53, 65, and 77°C correlate to distinct structural alterations of the Corneal Stroma. At higher temperatures, the SHG intensity decreases and a baseline in SHG signal is reached at 90°C. Our results demonstrate that SHG microscopy is a useful technique for obtaining qualitative and quantitative information of thermally treated Corneal fibers without histological or labeling procedures. With additional developments, SHG imaging may be developed into an effective imaging technique for in vivo characterization of Cornea structural changes.
Sung-jan Lin - One of the best experts on this subject based on the ideXlab platform.
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Multiphoton autofluorescence and second-harmonic generation imaging of the ex vivo porcine eye.
Investigative ophthalmology & visual science, 2006Co-Authors: Shu-wen Teng, Ju-li Peng, Huei-hsing Lin, Yen Sun, Wei-chou Lin, Sung-jan Lin, Hsin-yuan Tan, Ki Hean Kim, Shiou-hwa JeeAbstract:PURPOSE. The purpose of this work was to demonstrate the use of the combined imaging modality of multiphoton autofluorescence and second-harmonic generation (SHG) microscopy in obtaining spectrally resolved morphologic features of the Cornea, limbus, conjunctiva, and sclera in whole, ex vivo porcine eyes. METHODS. The 780-nm output of a femtosecond, titanium-sapphire laser was used to induce broadband autofluorescence (435-700 nm) and SHG (390 nm) from various regions of the surface of ex vivo porcine eyes. A water-immersion objective was used for convenient imaging of the curved surface of the eye. RESULTS. Multiphoton autofluorescence was useful in identifying cellular structures of the different domains of the ocular surface, and the SHG signal can be used to resolve collagen organization within the Cornea Stroma and sclera of ex vivo porcine eyes. CONCLUSIONS. Multiphoton autofluorescence and SHG microscopy have been demonstrated to be an effective technique for resolving, respectively, the cellular and collagen structures within the ocular surface of ex vivo porcine eyes. SHG imaging resolved the difference in structural orientations between Corneal and sclera collagen fibers. Specifically, the Corneal collagen is organized in a depth-dependent fashion, whereas the scleral collagen is randomly packed. Because this technique does not require histologic preparation procedures, it has the potential to be applied for in vivo studies with minimal disturbance to the eye.
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characterizing the thermally induced structural changes to intact porcine eye part 1 second harmonic generation imaging of Cornea Stroma
Journal of Biomedical Optics, 2005Co-Authors: Hsin-yuan Tan, Shu-wen Teng, Wei-chou Lin, Sung-jan Lin, Shiou-hwa Jee, Chenyuan DongAbstract:We characterize the structural changes of porcine Corneal structures from 25 to 90°C using second harmonic generation (SHG) microscopy. Our results show that porcine Stroma undergoes several distinct stages of structural changes between 25 and 90°C. A decrease in SHG intensity from 30 to 45°C and the existence of SHG intensity peaks at 53, 65, and 77°C correlate to distinct structural alterations of the Corneal Stroma. At higher temperatures, the SHG intensity decreases and a baseline in SHG signal is reached at 90°C. Our results demonstrate that SHG microscopy is a useful technique for obtaining qualitative and quantitative information of thermally treated Corneal fibers without histological or labeling procedures. With additional developments, SHG imaging may be developed into an effective imaging technique for in vivo characterization of Cornea structural changes.