The Experts below are selected from a list of 6057 Experts worldwide ranked by ideXlab platform
Ruchire Eranga Wijesinghe - One of the best experts on this subject based on the ideXlab platform.
-
Biocompatibility evaluation of bioprinted decellularized collagen sheet implanted in vivo Cornea using swept‐source optical coherence tomography
Journal of Biophotonics, 2019Co-Authors: Jaeseok Park, Sungjo Park, Ruchire Eranga WijesingheAbstract:Corneal transplantation by full-Thickness penetrating keratoplasty with human donor tissue is a widely accepted treatment for damaged or diseased Corneas. Although Corneal transplantation has a high success rate, a shortage of high-quality donor tissue is a considerable limitation. Therefore, bioengineered Corneas could be an effective solution for this limitation, and a decellularized extracellular matrix comprises a promising scaffold for their fabrication. In this study, three-dimensional bioprinted decellularized collagen sheets were implanted into the stromal layer of the Cornea of five rabbits. We performed in vivo noninvasive monitoring of the rabbit Corneas using swept-source optical coherence tomography (OCT) after implanting the collagen sheets. Anterior segment OCT images and averaged amplitude-scans were acquired biweekly to monitor Corneal Thickness after implantation for 1 month. The averaged Cornea Thickness in the control images was 430.3 ± 5.9 μm, while the averaged Thickness after Corneal implantation was 598.5 ± 11.8 μm and 564.5 ± 12.5 μm at 2 and 4 weeks, respectively. The Corneal Thickness reduction of 34 μm confirmed the biocompatibility through the image analysis of the depth-intensity profile base. Moreover, hematoxylin and eosin staining supported the biocompatibility evaluation of the bioprinted decellularized collagen sheet implantation. Hence, the developed bioprinted decellularized collagen sheets could become an alternative solution to human Corneal donor tissue, and the proposed image analysis procedure could be beneficial to confirm the success of the surgery.
-
Biocompatibility evaluation of bioprinted decellularized collagen sheet implanted in vivo Cornea using swept-source optical coherence tomography.
Journal of Biophotonics, 2019Co-Authors: Jaeseok Park, Sungjo Park, Ruchire Eranga WijesingheAbstract:Corneal transplantation by full-Thickness penetrating keratoplasty with human donor tissue is a widely accepted treatment for damaged or diseased Corneas. Although Corneal transplantation has a high success rate, a shortage of high-quality donor tissue is a considerable limitation. Therefore, bioengineered Corneas could be an effective solution for this limitation, and a decellularized extracellular matrix comprises a promising scaffold for their fabrication. In this study, three-dimensional bioprinted decellularized collagen sheets were implanted into the stromal layer of the Cornea of five rabbits. We performed in vivo noninvasive monitoring of the rabbit Corneas using swept-source optical coherence tomography (OCT) after implanting the collagen sheets. Anterior segment OCT images and averaged amplitude-scans were acquired biweekly to monitor Corneal Thickness after implantation for 1 month. The averaged Cornea Thickness in the control images was 430.3 ± 5.9 μm, while the averaged Thickness after Corneal implantation was 598.5 ± 11.8 μm and 564.5 ± 12.5 μm at 2 and 4 weeks, respectively. The Corneal Thickness reduction of 34 μm confirmed the biocompatibility through the image analysis of the depth-intensity profile base. Moreover, hematoxylin and eosin staining supported the biocompatibility evaluation of the bioprinted decellularized collagen sheet implantation. Hence, the developed bioprinted decellularized collagen sheets could become an alternative solution to human Corneal donor tissue, and the proposed image analysis procedure could be beneficial to confirm the success of the surgery.
Jaeseok Park - One of the best experts on this subject based on the ideXlab platform.
-
Biocompatibility evaluation of bioprinted decellularized collagen sheet implanted in vivo Cornea using swept‐source optical coherence tomography
Journal of Biophotonics, 2019Co-Authors: Jaeseok Park, Sungjo Park, Ruchire Eranga WijesingheAbstract:Corneal transplantation by full-Thickness penetrating keratoplasty with human donor tissue is a widely accepted treatment for damaged or diseased Corneas. Although Corneal transplantation has a high success rate, a shortage of high-quality donor tissue is a considerable limitation. Therefore, bioengineered Corneas could be an effective solution for this limitation, and a decellularized extracellular matrix comprises a promising scaffold for their fabrication. In this study, three-dimensional bioprinted decellularized collagen sheets were implanted into the stromal layer of the Cornea of five rabbits. We performed in vivo noninvasive monitoring of the rabbit Corneas using swept-source optical coherence tomography (OCT) after implanting the collagen sheets. Anterior segment OCT images and averaged amplitude-scans were acquired biweekly to monitor Corneal Thickness after implantation for 1 month. The averaged Cornea Thickness in the control images was 430.3 ± 5.9 μm, while the averaged Thickness after Corneal implantation was 598.5 ± 11.8 μm and 564.5 ± 12.5 μm at 2 and 4 weeks, respectively. The Corneal Thickness reduction of 34 μm confirmed the biocompatibility through the image analysis of the depth-intensity profile base. Moreover, hematoxylin and eosin staining supported the biocompatibility evaluation of the bioprinted decellularized collagen sheet implantation. Hence, the developed bioprinted decellularized collagen sheets could become an alternative solution to human Corneal donor tissue, and the proposed image analysis procedure could be beneficial to confirm the success of the surgery.
-
Biocompatibility evaluation of bioprinted decellularized collagen sheet implanted in vivo Cornea using swept-source optical coherence tomography.
Journal of Biophotonics, 2019Co-Authors: Jaeseok Park, Sungjo Park, Ruchire Eranga WijesingheAbstract:Corneal transplantation by full-Thickness penetrating keratoplasty with human donor tissue is a widely accepted treatment for damaged or diseased Corneas. Although Corneal transplantation has a high success rate, a shortage of high-quality donor tissue is a considerable limitation. Therefore, bioengineered Corneas could be an effective solution for this limitation, and a decellularized extracellular matrix comprises a promising scaffold for their fabrication. In this study, three-dimensional bioprinted decellularized collagen sheets were implanted into the stromal layer of the Cornea of five rabbits. We performed in vivo noninvasive monitoring of the rabbit Corneas using swept-source optical coherence tomography (OCT) after implanting the collagen sheets. Anterior segment OCT images and averaged amplitude-scans were acquired biweekly to monitor Corneal Thickness after implantation for 1 month. The averaged Cornea Thickness in the control images was 430.3 ± 5.9 μm, while the averaged Thickness after Corneal implantation was 598.5 ± 11.8 μm and 564.5 ± 12.5 μm at 2 and 4 weeks, respectively. The Corneal Thickness reduction of 34 μm confirmed the biocompatibility through the image analysis of the depth-intensity profile base. Moreover, hematoxylin and eosin staining supported the biocompatibility evaluation of the bioprinted decellularized collagen sheet implantation. Hence, the developed bioprinted decellularized collagen sheets could become an alternative solution to human Corneal donor tissue, and the proposed image analysis procedure could be beneficial to confirm the success of the surgery.
Lutz E. Pillunat - One of the best experts on this subject based on the ideXlab platform.
-
Measurement of the intraocular pressure with the “transpalpebral tonometer” TGDc-01 in comparison with applanation tonometry
Graefe's Archive for Clinical and Experimental Ophthalmology, 2005Co-Authors: Dirk Sandner, A. Böhm, Sandra Kostov, Lutz E. PillunatAbstract:Background An irregular Corneal surface compromises IOP measurement by Goldmann applanation tonometry. In such cases accurate measurement without Corneal contact would be desirable. The new eyelid tonometer TGDc-01 measures IOP without Corneal contact through the eyelid. The aim of the study was to evaluate the accuracy of the eyelid tonometer compared with Goldmann applanation tonometry (Cornea Thickness-corrected values) in subjects without Corneal alterations. Methods IOP was measured in 199 eyes of 103 subjects without Corneal alterations by means of two different methods. Measurements with the transpalpebral tonometer TGDc-01 and the Goldmann applanation tonometer were performed within 5 min in random order. Results The mean difference between lid tonometry and Goldmann applanation tonometry was 0.71 mmHg, SD ±2.467 mmHg. In the reliability analysis the intraclass correlation coefficient was 0.8620. Compared with Goldmann applanation tonometry 66.4% of the IOP readings measured by lid tonometry were in an interval of ±2 mmHg, 81.0% in an interval of ±3 mmHg. The maximum of deviation was −6 mmHg and +6 mmHg, respectively. The Bland and Altman plots are shown. Conclusions Lid tonometry correlates sufficiently with Goldmann applanation tonometry, but in more than 10% of the measurements the IOP readings differed by more than 3 mmHg. The eyelid tonometer may be helpful as a screening tool when Goldmann applanation tonometry is not applicable.
-
Measurement of the intraocular pressure with the "transpalpebral tonometer" TGDc-01 in comparison with applanation tonometry.
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 2004Co-Authors: Dirk Sandner, A. Böhm, Sandra Kostov, Lutz E. PillunatAbstract:Background An irregular Corneal surface compromises IOP measurement by Goldmann applanation tonometry. In such cases accurate measurement without Corneal contact would be desirable. The new eyelid tonometer TGDc-01 measures IOP without Corneal contact through the eyelid. The aim of the study was to evaluate the accuracy of the eyelid tonometer compared with Goldmann applanation tonometry (Cornea Thickness-corrected values) in subjects without Corneal alterations.
Jin U Kang - One of the best experts on this subject based on the ideXlab platform.
-
optical coherence tomography guided robotic device for autonomous needle insertion in Cornea transplant surgery
Intelligent Robots and Systems, 2019Co-Authors: Shoujing Guo, Nicolas R Sarfaraz, William G Gensheimer, Axel Krieger, Jin U KangAbstract:This paper reports the design and evaluation of a novel robotic device for Cornea transplant surgery. The device enables the OCT-sensor guided Big Bubble hydro-dissection approach for deep anterior lamellar keratoplasty (DALK) Cornea transplant surgery. DALK is highly challenging because it requires precise placement of a needle into the stroma of the Cornea down to Descemets Membrane (DM) and injects a fluid to separate the remaining stroma from Descemet’s membrane. Finally, the stroma is removed and replaced with the donor Cornea graft. Compared to traditional penetrating keratoplasty (PK), which involves a full-Thickness graft, this method significantly reduces the risk of rejection of the donor Cornea by keeping the DM intact. A comparison of autonomous OCT guided needle insertions with expert manual needle insertions showed that the device significantly increased the precision and consistency of the needle placement, which could lead to better visual outcomes and fewer complications. In a study on cadaver porcine eyes, the measured insertion depth as a percentage of Cornea Thickness for the robotic device was 90.05% +/- 2.33% compared to 79.16% +/- 5.68% for manual insertions.
-
IROS - Optical Coherence Tomography Guided Robotic Device for Autonomous Needle Insertion in Cornea Transplant Surgery
2019 IEEE RSJ International Conference on Intelligent Robots and Systems (IROS), 2019Co-Authors: Shoujing Guo, Nicolas R Sarfaraz, William G Gensheimer, Axel Krieger, Jin U KangAbstract:This paper reports the design and evaluation of a novel robotic device for Cornea transplant surgery. The device enables the OCT-sensor guided Big Bubble hydro-dissection approach for deep anterior lamellar keratoplasty (DALK) Cornea transplant surgery. DALK is highly challenging because it requires precise placement of a needle into the stroma of the Cornea down to Descemets Membrane (DM) and injects a fluid to separate the remaining stroma from Descemet’s membrane. Finally, the stroma is removed and replaced with the donor Cornea graft. Compared to traditional penetrating keratoplasty (PK), which involves a full-Thickness graft, this method significantly reduces the risk of rejection of the donor Cornea by keeping the DM intact. A comparison of autonomous OCT guided needle insertions with expert manual needle insertions showed that the device significantly increased the precision and consistency of the needle placement, which could lead to better visual outcomes and fewer complications. In a study on cadaver porcine eyes, the measured insertion depth as a percentage of Cornea Thickness for the robotic device was 90.05% +/- 2.33% compared to 79.16% +/- 5.68% for manual insertions.
Sungjo Park - One of the best experts on this subject based on the ideXlab platform.
-
Biocompatibility evaluation of bioprinted decellularized collagen sheet implanted in vivo Cornea using swept‐source optical coherence tomography
Journal of Biophotonics, 2019Co-Authors: Jaeseok Park, Sungjo Park, Ruchire Eranga WijesingheAbstract:Corneal transplantation by full-Thickness penetrating keratoplasty with human donor tissue is a widely accepted treatment for damaged or diseased Corneas. Although Corneal transplantation has a high success rate, a shortage of high-quality donor tissue is a considerable limitation. Therefore, bioengineered Corneas could be an effective solution for this limitation, and a decellularized extracellular matrix comprises a promising scaffold for their fabrication. In this study, three-dimensional bioprinted decellularized collagen sheets were implanted into the stromal layer of the Cornea of five rabbits. We performed in vivo noninvasive monitoring of the rabbit Corneas using swept-source optical coherence tomography (OCT) after implanting the collagen sheets. Anterior segment OCT images and averaged amplitude-scans were acquired biweekly to monitor Corneal Thickness after implantation for 1 month. The averaged Cornea Thickness in the control images was 430.3 ± 5.9 μm, while the averaged Thickness after Corneal implantation was 598.5 ± 11.8 μm and 564.5 ± 12.5 μm at 2 and 4 weeks, respectively. The Corneal Thickness reduction of 34 μm confirmed the biocompatibility through the image analysis of the depth-intensity profile base. Moreover, hematoxylin and eosin staining supported the biocompatibility evaluation of the bioprinted decellularized collagen sheet implantation. Hence, the developed bioprinted decellularized collagen sheets could become an alternative solution to human Corneal donor tissue, and the proposed image analysis procedure could be beneficial to confirm the success of the surgery.
-
Biocompatibility evaluation of bioprinted decellularized collagen sheet implanted in vivo Cornea using swept-source optical coherence tomography.
Journal of Biophotonics, 2019Co-Authors: Jaeseok Park, Sungjo Park, Ruchire Eranga WijesingheAbstract:Corneal transplantation by full-Thickness penetrating keratoplasty with human donor tissue is a widely accepted treatment for damaged or diseased Corneas. Although Corneal transplantation has a high success rate, a shortage of high-quality donor tissue is a considerable limitation. Therefore, bioengineered Corneas could be an effective solution for this limitation, and a decellularized extracellular matrix comprises a promising scaffold for their fabrication. In this study, three-dimensional bioprinted decellularized collagen sheets were implanted into the stromal layer of the Cornea of five rabbits. We performed in vivo noninvasive monitoring of the rabbit Corneas using swept-source optical coherence tomography (OCT) after implanting the collagen sheets. Anterior segment OCT images and averaged amplitude-scans were acquired biweekly to monitor Corneal Thickness after implantation for 1 month. The averaged Cornea Thickness in the control images was 430.3 ± 5.9 μm, while the averaged Thickness after Corneal implantation was 598.5 ± 11.8 μm and 564.5 ± 12.5 μm at 2 and 4 weeks, respectively. The Corneal Thickness reduction of 34 μm confirmed the biocompatibility through the image analysis of the depth-intensity profile base. Moreover, hematoxylin and eosin staining supported the biocompatibility evaluation of the bioprinted decellularized collagen sheet implantation. Hence, the developed bioprinted decellularized collagen sheets could become an alternative solution to human Corneal donor tissue, and the proposed image analysis procedure could be beneficial to confirm the success of the surgery.