The Experts below are selected from a list of 10434 Experts worldwide ranked by ideXlab platform
Sumio Terada - One of the best experts on this subject based on the ideXlab platform.
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direct label free measurement of the distribution of small molecular weight compound inside thick biological Tissue using coherent raman microspectroscopy
Biophysical Journal, 2016Co-Authors: Masahiko Kawagishi, Yuki Obara, Takayuki Suzuki, Masumi Hayashi, Kazuhiko Misawa, Sumio TeradaAbstract:Distributions of small molecular weight (less than 300 Da) compounds inside biological Tissue have been obscure because of the lack of appropriate methods to measure them. Although fluorescence techniques are widely used to characterise the localisation of large biomolecules, they cannot be easily applied to the cases with small molecule compounds. We used CARS spectroscopy to detect and identify a label-free small molecule compound. To facilitate detection in aqueous environment, we utilised time-resolved and phase-sensitive techniques to reduce non-resonant background generated from water. We applied this technique to detect small molecular weight compound, taurine, inside mouse Cornea Tissue immersed in taurine solution as an initial model experiment. We detected a Raman peak of taurine near wavenumber 1033 /cm inside Cornea and successfully characterised its depth profile in the Tissue. Our CARS spectra measurement can be a promising method to measure and visualise the distribution of small bio-related compounds in biological background without using any labeling, paving the way for new cell biological analysis in various disciplines.Sci. Rep. 5, 13868; http://dx.doi.org/10.1038/srep13868 (2015).
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direct label free measurement of the distribution of small molecular weight compound inside thick biological Tissue using coherent raman microspectroscopy
Scientific Reports, 2015Co-Authors: Masahiko Kawagishi, Yuki Obara, Takayuki Suzuki, Masumi Hayashi, Kazuhiko Misawa, Sumio TeradaAbstract:Distributions of small molecular weight (less than 300 Da) compounds inside biological Tissue have been obscure because of the lack of appropriate methods to measure them. Although fluorescence techniques are widely used to characterise the localisation of large biomolecules, they cannot be easily applied to the cases with small molecule compounds. We used CARS spectroscopy to detect and identify a label-free small molecule compound. To facilitate detection in aqueous environment, we utilised time-resolved and phase-sensitive techniques to reduce non-resonant background generated from water. We applied this technique to detect small molecular weight compound, taurine, inside mouse Cornea Tissue immersed in taurine solution as an initial model experiment. We detected a Raman peak of taurine near wavenumber 1033 cm−1 inside Cornea and successfully characterised its depth profile in the Tissue. Our CARS spectra measurement can be a promising method to measure and visualise the distribution of small bio-related compounds in biological background without using any labeling, paving the way for new cell biological analysis in various disciplines.
David L Kaplan - One of the best experts on this subject based on the ideXlab platform.
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multi layered silk film coculture system for human Corneal epithelial and stromal stem cells
Journal of Tissue Engineering and Regenerative Medicine, 2018Co-Authors: Emily A Gosselin, Chiara E Ghezzi, Tess Torregrosa, Alexandra C Mendelsohn, Rachel Gomes, James L Funderburgh, David L KaplanAbstract:With insufficient options to meet the clinical demand for Cornea transplants, one emerging area of emphasis is on Cornea Tissue engineering. In the present study, the goal was to combine the Corneal stroma and epithelium into one co-culture system, in order to monitor both human Corneal stromal stem cell (hCSSC) and human Corneal epithelial cell (hCE) growth and differentiation into keratocytes and differentiated epithelium in these 3D Tissue systems in vitro. Co-culture conditions were first optimized, including the medium, air/liquid interface culture, and surface topography and chemistry of biomaterial scaffold films based on silk protein. The silk was used as scaffolding for both stromal and epithelial Tissue layers because it is cell compatible, can be surface patterned, and is optically clear. Next, the effects of proliferating and differentiating hCEs and hCSSCs in this in vitro system were studied, including the effects on cell proliferation, matrix formation by immunochemistry, and gene expression by RT-qPCR. The incorporation of both cell types into the co-culture system demonstrated more complete differentiation and growth for both cell types compared to the Corneal stromal cells and Corneal epithelial cells alone. Silk films for Corneal epithelial culture were optimized to combine a 4.0 micron-scale surface pattern with bulk-loaded collagen type IV. Differentiation of each cell type was in evidence based on increased expression of Corneal stroma and epithelial proteins and transcript levels after 6 weeks in co-culture on the optimized silk scaffolds. This article is protected by copyright. All rights reserved.
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Coculture of dorsal root ganglion neurons and differentiated human Corneal stromal stem cells on silk‐based scaffolds
Journal of biomedical materials research. Part A, 2015Co-Authors: Siran Wang, Chiara E Ghezzi, James D. White, David L KaplanAbstract:Corneal Tissue displays the highest peripheral nerve density in the human body. Engineering of biomaterials to promote interactions between neurons and Corneal Tissue could provide Tissue models for nerve/Cornea development, platforms for drug screening, as well as innovative opportunities to regenerate Cornea Tissue. The focus of this study was to develop a coculture system for differentiated human Corneal stromal stem cells (dhCSSCs) and dorsal root ganglion neurons (DRG) to mimic the human Cornea Tissue interactions. Axon extension, connectivity, and neuron cell viability were studied. DRG neurons developed longer axons when cocultured with dhCSSCs in comparison to neuron cultures alone. To assess the mechanism involved in the coculture response, nerve growth factors (NGF) secreted by dhCSSCs including NGF, brain-derived neurotrophic factor (BDNF), glial cell-derived neurotrophic factor (GDNF), and neurotrophin-3 were characterized with greater focus on BDNF secretion. DhCSSCs also secreted collagen type I, an extracellular matrix molecule favorable for neuronal outgrowth. This coculture system provides a slowly degrading silk matrix to study neuronal responses in concert with hCSSCs related to innervation of Corneal Tissue with utility toward human Corneal nerve regeneration and associated diseases.
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Corneal Tissue Engineering: Recent Advances and Future Perspectives
Tissue Engineering Part B: Reviews, 2014Co-Authors: Chiara E Ghezzi, Jelena Rnjak-kovacina, David L KaplanAbstract:To address the growing need for Corneal transplants two main approaches are being pursued: allogenic and synthetic materials. Allogenic Tissue from human donors is currently the preferred choice; however, there is a worldwide shortage in donated Corneal Tissue. In addition, Tissue rejection often limits the long-term success of this approach. Alternatively, synthetic homologs to donor Corneal grafts are primarily considered temporary replacements until suitable donor Tissue becomes available, as they result in a high incidence of graft failure. Tissue engineered Cornea analogs would provide effective Cornea Tissue substitutes and alternatives to address the need to reduce animal testing of commercial products. Recent progress toward these needs is reviewed here, along with future perspectives.
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Silk film biomaterials for Cornea Tissue engineering
Biomaterials, 2008Co-Authors: Brian Lawrence, Jeffrey K. Marchant, Mariya A. Pindrus, Fiorenzo G. Omenetto, David L KaplanAbstract:Abstract Biomaterials for Corneal Tissue engineering must demonstrate several critical features for potential utility in vivo, including transparency, mechanical integrity, biocompatibility and slow biodegradation. Silk film biomaterials were designed and characterized to meet these functional requirements. Silk protein films were used in a biomimetic approach to replicate Corneal stromal Tissue architecture. The films were 2 μm thick to emulate Corneal collagen lamellae dimensions, and were surface patterned to guide cell alignment. To enhance trans-lamellar diffusion of nutrients and to promote cell–cell interaction, pores with 0.5–5.0 μm diameters were introduced into the silk films. Human and rabbit Corneal fibroblast proliferation, alignment and Corneal extracellular matrix expression on these films in both 2D and 3D cultures were demonstrated. The mechanical properties, optical clarity and surface patterned features of these films, combined with their ability to support Corneal cell functions suggest that this new biomaterial system offers important potential benefits for Corneal Tissue regeneration.
Masahiko Kawagishi - One of the best experts on this subject based on the ideXlab platform.
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direct label free measurement of the distribution of small molecular weight compound inside thick biological Tissue using coherent raman microspectroscopy
Biophysical Journal, 2016Co-Authors: Masahiko Kawagishi, Yuki Obara, Takayuki Suzuki, Masumi Hayashi, Kazuhiko Misawa, Sumio TeradaAbstract:Distributions of small molecular weight (less than 300 Da) compounds inside biological Tissue have been obscure because of the lack of appropriate methods to measure them. Although fluorescence techniques are widely used to characterise the localisation of large biomolecules, they cannot be easily applied to the cases with small molecule compounds. We used CARS spectroscopy to detect and identify a label-free small molecule compound. To facilitate detection in aqueous environment, we utilised time-resolved and phase-sensitive techniques to reduce non-resonant background generated from water. We applied this technique to detect small molecular weight compound, taurine, inside mouse Cornea Tissue immersed in taurine solution as an initial model experiment. We detected a Raman peak of taurine near wavenumber 1033 /cm inside Cornea and successfully characterised its depth profile in the Tissue. Our CARS spectra measurement can be a promising method to measure and visualise the distribution of small bio-related compounds in biological background without using any labeling, paving the way for new cell biological analysis in various disciplines.Sci. Rep. 5, 13868; http://dx.doi.org/10.1038/srep13868 (2015).
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direct label free measurement of the distribution of small molecular weight compound inside thick biological Tissue using coherent raman microspectroscopy
Scientific Reports, 2015Co-Authors: Masahiko Kawagishi, Yuki Obara, Takayuki Suzuki, Masumi Hayashi, Kazuhiko Misawa, Sumio TeradaAbstract:Distributions of small molecular weight (less than 300 Da) compounds inside biological Tissue have been obscure because of the lack of appropriate methods to measure them. Although fluorescence techniques are widely used to characterise the localisation of large biomolecules, they cannot be easily applied to the cases with small molecule compounds. We used CARS spectroscopy to detect and identify a label-free small molecule compound. To facilitate detection in aqueous environment, we utilised time-resolved and phase-sensitive techniques to reduce non-resonant background generated from water. We applied this technique to detect small molecular weight compound, taurine, inside mouse Cornea Tissue immersed in taurine solution as an initial model experiment. We detected a Raman peak of taurine near wavenumber 1033 cm−1 inside Cornea and successfully characterised its depth profile in the Tissue. Our CARS spectra measurement can be a promising method to measure and visualise the distribution of small bio-related compounds in biological background without using any labeling, paving the way for new cell biological analysis in various disciplines.
Kazuhiko Misawa - One of the best experts on this subject based on the ideXlab platform.
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direct label free measurement of the distribution of small molecular weight compound inside thick biological Tissue using coherent raman microspectroscopy
Biophysical Journal, 2016Co-Authors: Masahiko Kawagishi, Yuki Obara, Takayuki Suzuki, Masumi Hayashi, Kazuhiko Misawa, Sumio TeradaAbstract:Distributions of small molecular weight (less than 300 Da) compounds inside biological Tissue have been obscure because of the lack of appropriate methods to measure them. Although fluorescence techniques are widely used to characterise the localisation of large biomolecules, they cannot be easily applied to the cases with small molecule compounds. We used CARS spectroscopy to detect and identify a label-free small molecule compound. To facilitate detection in aqueous environment, we utilised time-resolved and phase-sensitive techniques to reduce non-resonant background generated from water. We applied this technique to detect small molecular weight compound, taurine, inside mouse Cornea Tissue immersed in taurine solution as an initial model experiment. We detected a Raman peak of taurine near wavenumber 1033 /cm inside Cornea and successfully characterised its depth profile in the Tissue. Our CARS spectra measurement can be a promising method to measure and visualise the distribution of small bio-related compounds in biological background without using any labeling, paving the way for new cell biological analysis in various disciplines.Sci. Rep. 5, 13868; http://dx.doi.org/10.1038/srep13868 (2015).
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direct label free measurement of the distribution of small molecular weight compound inside thick biological Tissue using coherent raman microspectroscopy
Scientific Reports, 2015Co-Authors: Masahiko Kawagishi, Yuki Obara, Takayuki Suzuki, Masumi Hayashi, Kazuhiko Misawa, Sumio TeradaAbstract:Distributions of small molecular weight (less than 300 Da) compounds inside biological Tissue have been obscure because of the lack of appropriate methods to measure them. Although fluorescence techniques are widely used to characterise the localisation of large biomolecules, they cannot be easily applied to the cases with small molecule compounds. We used CARS spectroscopy to detect and identify a label-free small molecule compound. To facilitate detection in aqueous environment, we utilised time-resolved and phase-sensitive techniques to reduce non-resonant background generated from water. We applied this technique to detect small molecular weight compound, taurine, inside mouse Cornea Tissue immersed in taurine solution as an initial model experiment. We detected a Raman peak of taurine near wavenumber 1033 cm−1 inside Cornea and successfully characterised its depth profile in the Tissue. Our CARS spectra measurement can be a promising method to measure and visualise the distribution of small bio-related compounds in biological background without using any labeling, paving the way for new cell biological analysis in various disciplines.
Masumi Hayashi - One of the best experts on this subject based on the ideXlab platform.
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direct label free measurement of the distribution of small molecular weight compound inside thick biological Tissue using coherent raman microspectroscopy
Biophysical Journal, 2016Co-Authors: Masahiko Kawagishi, Yuki Obara, Takayuki Suzuki, Masumi Hayashi, Kazuhiko Misawa, Sumio TeradaAbstract:Distributions of small molecular weight (less than 300 Da) compounds inside biological Tissue have been obscure because of the lack of appropriate methods to measure them. Although fluorescence techniques are widely used to characterise the localisation of large biomolecules, they cannot be easily applied to the cases with small molecule compounds. We used CARS spectroscopy to detect and identify a label-free small molecule compound. To facilitate detection in aqueous environment, we utilised time-resolved and phase-sensitive techniques to reduce non-resonant background generated from water. We applied this technique to detect small molecular weight compound, taurine, inside mouse Cornea Tissue immersed in taurine solution as an initial model experiment. We detected a Raman peak of taurine near wavenumber 1033 /cm inside Cornea and successfully characterised its depth profile in the Tissue. Our CARS spectra measurement can be a promising method to measure and visualise the distribution of small bio-related compounds in biological background without using any labeling, paving the way for new cell biological analysis in various disciplines.Sci. Rep. 5, 13868; http://dx.doi.org/10.1038/srep13868 (2015).
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direct label free measurement of the distribution of small molecular weight compound inside thick biological Tissue using coherent raman microspectroscopy
Scientific Reports, 2015Co-Authors: Masahiko Kawagishi, Yuki Obara, Takayuki Suzuki, Masumi Hayashi, Kazuhiko Misawa, Sumio TeradaAbstract:Distributions of small molecular weight (less than 300 Da) compounds inside biological Tissue have been obscure because of the lack of appropriate methods to measure them. Although fluorescence techniques are widely used to characterise the localisation of large biomolecules, they cannot be easily applied to the cases with small molecule compounds. We used CARS spectroscopy to detect and identify a label-free small molecule compound. To facilitate detection in aqueous environment, we utilised time-resolved and phase-sensitive techniques to reduce non-resonant background generated from water. We applied this technique to detect small molecular weight compound, taurine, inside mouse Cornea Tissue immersed in taurine solution as an initial model experiment. We detected a Raman peak of taurine near wavenumber 1033 cm−1 inside Cornea and successfully characterised its depth profile in the Tissue. Our CARS spectra measurement can be a promising method to measure and visualise the distribution of small bio-related compounds in biological background without using any labeling, paving the way for new cell biological analysis in various disciplines.