The Experts below are selected from a list of 27 Experts worldwide ranked by ideXlab platform

Cathy J Hatcher - One of the best experts on this subject based on the ideXlab platform.

  • tbx5 is required for avian and mammalian epicardial formation and Coronary vasculogenesis
    Circulation Research, 2014
    Co-Authors: Nata Y Diman, Gabriel Brooks, Boudewijn P T Kruithof, Olivier Elemento, J G Seidman, Christine E Seidman, Craig T Basson, Cathy J Hatcher
    Abstract:

    Rationale:Holt–Oram syndrome is an autosomal dominant heart-hand syndrome caused by mutations in the TBX5 gene. Overexpression of Tbx5 in the chick proepicardial organ impaired Coronary Blood Vessel formation. However, the potential activity of Tbx5 in the epicardium itself, and the role of Tbx5 in mammalian Coronary vasculogenesis, remains largely unknown. Objective:To evaluate the consequences of altered Tbx5 gene dosage during proepicardial organ and epicardial development in the embryonic chick and mouse. Methods and Results:Retroviral-mediated knockdown or upregulation of Tbx5 expression in the embryonic chick proepicardial organ and proepicardial-specific deletion of Tbx5 in the embryonic mouse (Tbx5epi-/) impaired normal proepicardial organ cell development, inhibited epicardial and Coronary Blood Vessel formation, and altered developmental gene expression. The generation of epicardial-derived cells and their migration into the myocardium were impaired between embryonic day (E) 13.5 to 15.5 in muta...

David M Bader - One of the best experts on this subject based on the ideXlab platform.

  • bves: A novel gene expressed during Coronary Blood Vessel development.
    Developmental Biology, 1999
    Co-Authors: David E. Reese, Maja Zavaljevski, Nicole L. Streiff, David M Bader
    Abstract:

    We have used a subtractive method to clone novel messages enriched in the heart. Here we show that one such message, bves (Blood Vessel/epicardial substance) is a novel protein that is highly conserved between chicken and mouse. The bves message is detected at high levels in early chick hearts. Using anti-Bves antibodies, we show expression in cells of the proepicardial organ, migrating epicardium, epicardial-derived mesenchyme, and smooth muscle of the developing intracardiac arterial system, including the Coronary arteries. Our data suggest that Bves is an early marker of developing vascular smooth muscle cells. In addition, the expression pattern of Bves protein reveals the patterning of intracardiac vascular smooth muscle and possible insights into the cellular regulation of smooth muscle differentiation during vasculogenesis.

Nata Y Diman - One of the best experts on this subject based on the ideXlab platform.

  • tbx5 is required for avian and mammalian epicardial formation and Coronary vasculogenesis
    Circulation Research, 2014
    Co-Authors: Nata Y Diman, Gabriel Brooks, Boudewijn P T Kruithof, Olivier Elemento, J G Seidman, Christine E Seidman, Craig T Basson, Cathy J Hatcher
    Abstract:

    Rationale:Holt–Oram syndrome is an autosomal dominant heart-hand syndrome caused by mutations in the TBX5 gene. Overexpression of Tbx5 in the chick proepicardial organ impaired Coronary Blood Vessel formation. However, the potential activity of Tbx5 in the epicardium itself, and the role of Tbx5 in mammalian Coronary vasculogenesis, remains largely unknown. Objective:To evaluate the consequences of altered Tbx5 gene dosage during proepicardial organ and epicardial development in the embryonic chick and mouse. Methods and Results:Retroviral-mediated knockdown or upregulation of Tbx5 expression in the embryonic chick proepicardial organ and proepicardial-specific deletion of Tbx5 in the embryonic mouse (Tbx5epi-/) impaired normal proepicardial organ cell development, inhibited epicardial and Coronary Blood Vessel formation, and altered developmental gene expression. The generation of epicardial-derived cells and their migration into the myocardium were impaired between embryonic day (E) 13.5 to 15.5 in muta...

David E. Reese - One of the best experts on this subject based on the ideXlab platform.

  • bves: A novel gene expressed during Coronary Blood Vessel development.
    Developmental Biology, 1999
    Co-Authors: David E. Reese, Maja Zavaljevski, Nicole L. Streiff, David M Bader
    Abstract:

    We have used a subtractive method to clone novel messages enriched in the heart. Here we show that one such message, bves (Blood Vessel/epicardial substance) is a novel protein that is highly conserved between chicken and mouse. The bves message is detected at high levels in early chick hearts. Using anti-Bves antibodies, we show expression in cells of the proepicardial organ, migrating epicardium, epicardial-derived mesenchyme, and smooth muscle of the developing intracardiac arterial system, including the Coronary arteries. Our data suggest that Bves is an early marker of developing vascular smooth muscle cells. In addition, the expression pattern of Bves protein reveals the patterning of intracardiac vascular smooth muscle and possible insights into the cellular regulation of smooth muscle differentiation during vasculogenesis.

Christine E Seidman - One of the best experts on this subject based on the ideXlab platform.

  • tbx5 is required for avian and mammalian epicardial formation and Coronary vasculogenesis
    Circulation Research, 2014
    Co-Authors: Nata Y Diman, Gabriel Brooks, Boudewijn P T Kruithof, Olivier Elemento, J G Seidman, Christine E Seidman, Craig T Basson, Cathy J Hatcher
    Abstract:

    Rationale:Holt–Oram syndrome is an autosomal dominant heart-hand syndrome caused by mutations in the TBX5 gene. Overexpression of Tbx5 in the chick proepicardial organ impaired Coronary Blood Vessel formation. However, the potential activity of Tbx5 in the epicardium itself, and the role of Tbx5 in mammalian Coronary vasculogenesis, remains largely unknown. Objective:To evaluate the consequences of altered Tbx5 gene dosage during proepicardial organ and epicardial development in the embryonic chick and mouse. Methods and Results:Retroviral-mediated knockdown or upregulation of Tbx5 expression in the embryonic chick proepicardial organ and proepicardial-specific deletion of Tbx5 in the embryonic mouse (Tbx5epi-/) impaired normal proepicardial organ cell development, inhibited epicardial and Coronary Blood Vessel formation, and altered developmental gene expression. The generation of epicardial-derived cells and their migration into the myocardium were impaired between embryonic day (E) 13.5 to 15.5 in muta...