The Experts below are selected from a list of 150 Experts worldwide ranked by ideXlab platform
Ross J Baldessarini - One of the best experts on this subject based on the ideXlab platform.
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Localization of dopamine receptor subtypes in Corpus Striatum and nucleus accumbens septi of rat brain: comparison of D1-, D2-, and D4-like receptors.
Neuroscience, 1998Co-Authors: Frank I. Tarazi, Alexander Campbell, Sylva K. Yeghiayan, Ross J BaldessariniAbstract:Changes in D1-, D2- and D4-like dopamine receptor binding in rat brain were examined by quantitative autoradiography following: (i) unilateral surgical ablation of frontal cerebral cortex to remove descending projections to Corpus Striatum and nucleus accumbens, (ii) unilateral injections of kainic acid into Corpus Striatum or nucleus accumbens to degenerate local intrinsic neurons, (iii) unilateral injections of 6-hydroxydopamine into substantia nigra to degenerate ascending dopamine projections. Rats were killed one week after lesioning, with contralateral tissue controls. Radioligands were: [3H]SCH-23390 for D1-like (D1/D5) receptors, [3H]nemonapride alone for D2-like (D2/D3/D4) receptors, and [3H]nemonapride with 300 nM S[-]-raclopride and other masking agents for D4-like receptors (identified by blockade with D4 selective L-745,870). Frontal cerebral cortex ablation did not alter D1- or D2-like receptor density, but D4-like binding decreased significantly in both Corpus Striatum (18%) and nucleus accumbens (23%). Kainic acid markedly reduced D1-like (75% and 84%) and D2-like binding (44% and 52%), with smaller D4-like losses (28% and 27%) in Corpus Striatum and nucleus accumbens, respectively. Nigral 6-hydroxydopamine lesions (verified by autoradiographic loss of dopamine transporters labelled with [3H]GBR-12935) did not significantly change D1-, D2-, or D4-like binding in the Corpus Striatum. These results suggest that the majority of D1-, and D2-like, and a smaller portion of D4-like receptors in Corpus Striatum and nucleus accumbens arise on intrinsic postsynaptic neurons, and that some D4-like, but neither D1- nor D2-like, receptors are found on presynaptic corticostriatal afferents.
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(+)-6,7-benzomorphan sigma ligands stimulate dopamine synthesis in rat Corpus Striatum tissue
Brain Research, 1991Co-Authors: Raymond G Booth, Ross J BaldessariniAbstract:Abstract The benzomorphan δ ligands, (+)-N-allylnormetazocine (NANM) and (+)-pentazocine, but not (+)-cyclazocine, stereospecifically stimulated dopamine synthesis in minces of rat Corpus Striatum by 15–23% over basal values at 0.1–1.0 μM. The effect of (+)-NANM and (+)-pentazocine was blocked by the reported δ antagonist, BMY-14802 but not by the opiate antagonist naloxone. These results suggest that these (+)-benzomorphans may act as agonists at putative δ peteroreceptorson striatal nerve terminals, or through an indirect mechanism, to modulate dopamine synthesis.
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6 7 benzomorphan sigma ligands stimulate dopamine synthesis in rat Corpus Striatum tissue
Brain Research, 1991Co-Authors: Raymond G Booth, Ross J BaldessariniAbstract:Abstract The benzomorphan δ ligands, (+)-N-allylnormetazocine (NANM) and (+)-pentazocine, but not (+)-cyclazocine, stereospecifically stimulated dopamine synthesis in minces of rat Corpus Striatum by 15–23% over basal values at 0.1–1.0 μM. The effect of (+)-NANM and (+)-pentazocine was blocked by the reported δ antagonist, BMY-14802 but not by the opiate antagonist naloxone. These results suggest that these (+)-benzomorphans may act as agonists at putative δ peteroreceptorson striatal nerve terminals, or through an indirect mechanism, to modulate dopamine synthesis.
Raymond G Booth - One of the best experts on this subject based on the ideXlab platform.
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(+)-6,7-benzomorphan sigma ligands stimulate dopamine synthesis in rat Corpus Striatum tissue
Brain Research, 1991Co-Authors: Raymond G Booth, Ross J BaldessariniAbstract:Abstract The benzomorphan δ ligands, (+)-N-allylnormetazocine (NANM) and (+)-pentazocine, but not (+)-cyclazocine, stereospecifically stimulated dopamine synthesis in minces of rat Corpus Striatum by 15–23% over basal values at 0.1–1.0 μM. The effect of (+)-NANM and (+)-pentazocine was blocked by the reported δ antagonist, BMY-14802 but not by the opiate antagonist naloxone. These results suggest that these (+)-benzomorphans may act as agonists at putative δ peteroreceptorson striatal nerve terminals, or through an indirect mechanism, to modulate dopamine synthesis.
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6 7 benzomorphan sigma ligands stimulate dopamine synthesis in rat Corpus Striatum tissue
Brain Research, 1991Co-Authors: Raymond G Booth, Ross J BaldessariniAbstract:Abstract The benzomorphan δ ligands, (+)-N-allylnormetazocine (NANM) and (+)-pentazocine, but not (+)-cyclazocine, stereospecifically stimulated dopamine synthesis in minces of rat Corpus Striatum by 15–23% over basal values at 0.1–1.0 μM. The effect of (+)-NANM and (+)-pentazocine was blocked by the reported δ antagonist, BMY-14802 but not by the opiate antagonist naloxone. These results suggest that these (+)-benzomorphans may act as agonists at putative δ peteroreceptorson striatal nerve terminals, or through an indirect mechanism, to modulate dopamine synthesis.
C. S. Paulose - One of the best experts on this subject based on the ideXlab platform.
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Enhanced muscarinic M1 receptor gene expression in the Corpus Striatum of streptozotocin-induced diabetic rats
Journal of Biomedical Science, 2009Co-Authors: G. Gireesh, Jobin Mathew, T Peeyush Kumar, C. S. PauloseAbstract:Acetylcholine (ACh), the first neurotransmitter to be identified, regulate the activities of central and peripheral functions through interactions with muscarinic receptors. Changes in muscarinic acetylcholine receptor (mAChR) have been implicated in the pathophysiology of many major diseases of the central nervous system (CNS). Previous reports from our laboratory on streptozotocin (STZ) induced diabetic rats showed down regulation of muscarinic M1 receptors in the brainstem, hypothalamus, cerebral cortex and pancreatic islets. In this study, we have investigated the changes of acetylcholine esterase (AChE) enzyme activity, total muscarinic and muscarinic M1 receptor binding and gene expression in the Corpus Striatum of STZ – diabetic rats and the insulin treated diabetic rats. The Striatum, a neuronal nucleus intimately involved in motor behaviour, is one of the brain regions with the highest acetylcholine content. ACh has complex and clinically important actions in the Striatum that are mediated predominantly by muscarinic receptors. We observed that insulin treatment brought back the decreased maximal velocity (Vmax) of acetylcholine esterase in the Corpus Striatum during diabetes to near control state. In diabetic rats there was a decrease in maximal number (Bmax) and affinity (Kd) of total muscarinic receptors whereas muscarinic M1 receptors were increased with decrease in affinity in diabetic rats. We observed that, in all cases, the binding parameters were reversed to near control by the treatment of diabetic rats with insulin. Real-time PCR experiment confirmed the increase in muscarinic M1 receptor gene expression and a similar reversal with insulin treatment. These results suggest the diabetes-induced changes of the cholinergic activity in the Corpus Striatum and the regulatory role of insulin on binding parameters and gene expression of total and muscarinic M1 receptors.
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Enhanced 5-HT2A Receptor Status in the Hypothalamus and Corpus Striatum of Ethanol-Treated Rats
Cellular and molecular neurobiology, 2008Co-Authors: K. G. Akash, K. S. Balarama, C. S. PauloseAbstract:Aim Brain is the major target for the actions of ethanol and it can affect the brain in a variety of ways. In the present study we have investigated the changes in 5-HT level and the 5-HT2A receptors in the ethanol-treated rats. Methods Wistar adult male rats of 180–200 g body weight were given free access to 15% (v/v) (approx.7.5 g/Kg body wt./day) ethanol for 15 days. Controls were given free access to water for 15 days. Brain 5-HT and its metabolites were assayed by high performance liquid chromatography (HPLC) integrated with an electrochemical detector (ECD) fitted with C-18-CLS-ODS reverse phase column. 5-HT2A receptor binding assay was done with different concentrations of [3H] MDL 100907. Results The hypothalamic 5-HT content significantly increased (P < 0.001) with a decreased (P < 0.001) 5-HIAA/5-HT turnover in the ethanol-treated rats when compared to control. The Corpus Striatum 5-HT content significantly decreased (P < 0.01) with increased (P < 0.01) 5-HIAA/5- HT turnovers in the ethanol-treated rats when compared to control. Scatchard analysis of [3H] MDL 100907 against ketanserin in hypothalamus showed a significant increase (P < 0.001) in Bmax with a decreased affinity (P < 0.001) in ethanol-treated rats when compared to control. The competition curve for [3H] MDL 100907 against ketanserin fitted one-site model in all the groups with unity as Hill slope value. An increased Ki and log (EC50) value were also observed in ethanol-treated rats when compared to control. Scatchard analysis of [3H] MDL 100907 against ketanserin in the Corpus Striatum of ethanol-treated rats showed a significant increase (P < 0.001) in Bmax and in affinity (P < 0.01) when compared to control. The change in affinity of the receptor protein in both Corpus Striatum and hypothalamus shows an altered receptor. The competition curve for [3H] MDL 100907 against ketanserin fitted one-site model in all the groups with unity as Hill slope value. There was no significant change in Ki and log (EC 50) value in ethanol-treated rats when compared to control. Conclusion The present study demonstrated the enhanced 5-HT2A receptor status in hypothalamus and Corpus Striatum. The ethanol-induced enhanced 5-HT2A receptors in the hypothalamus and Corpus Striatum has clinical significance in the better management of ethanol addiction. This will have therapeutic application.
Lei Wang - One of the best experts on this subject based on the ideXlab platform.
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The Differentially Expressed Circular RNAs in the Substantia Nigra and Corpus Striatum of Nrf2-Knockout Mice.
Cellular physiology and biochemistry : international journal of experimental cellular physiology biochemistry and pharmacology, 2018Co-Authors: Jun-hui Yang, Huixian Cui, Shao-guang Sun, Run-jiao Zhang, Jia-jie Lin, Ming-chao Cao, Qie Wang, Lei WangAbstract:Background/Aims: The nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway plays a protective role in both acute neuronal damage and chronic neurodegeneration-related oxidative stress. Circular RNAs (circRNAs) are involved with various diseases in the central nervous system (CNS). This study aimed to identify the key circRNAs involved in Nrf2-neuroprotection against oxidative stress. Methods: The differentially expressed circRNAs (DEcircRNAs) in the substantia nigra and Corpus Striatum between Nrf2 (-/-) and Nrf2 (+/+) mice were identified by microarray analysis. Quantitative real-time polymerase chain reaction (qRT-PCR) was then used to validate the expression of selected DEcircRNAs in the substantia nigra and Corpus Striatum between Nrf2 (-/-) and Nrf2 (+/+) mice. Based on our previous microarray analysis of the differentially expressed mRNAs (DEmRNAs) in the substantia nigra and Corpus Striatum between Nrf2 (-/-) and Nrf2 (+/+) mice, the DEcircRNA-miRNA-DEmRNA interaction network was constructed. Functional annotation of DEmRNAs that shared the same binding miRNAs with DEcircRNAs was performed using gene ontology (GO) and pathway analyses. Results: A total of 65 and 150 significant DEcircRNAs were obtained in the substantia nigra and Corpus Striatum of Nrf2 (-/-) mice, respectively, and seventeen shared DEcircRNAs were found in both these two tissues. The qRT-PCR results were generally consistent with the microarray results. The DEcircRNA-miRNA-DEmRNA interaction network and pathway analysis indicated that mmu_circRNA_34132, mmu_circRNA_017077 and mmu-circRNA-015216 might be involved with Nrf2-mediated neuroprotection against oxidative stress. Mmu_circRNA_015216 and mmu_circRNA_017077 might play roles in the Nrf2-related transcriptional misregulation and Nrf2-mediated processes of rheumatoid arthritis, respectively. In addition to these two processes, mmu_circRNA_34132 may be a potential regulator of Nrf2-mediated protection for diabetes mellitus and Nrf2-mediated defence against ROS in hearts. Conclusion: In conclusion, our study identified the key DEcircRNAs in the substantia nigra and Corpus Striatum of Nrf2 (-/-) mice, which might provide new clues for further exploring the mechanism of Nrf2-mediated neuroprotection against oxidative stress and other Nrf2-mediated processes.
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The aberrantly expressed long non-coding RNA in the substantia nigra and Corpus Striatum of Nrf2-knockout mice.
Journal of neurochemistry, 2017Co-Authors: Jian Liu, Yunxiao Kang, Shanhu Cao, Geming Shi, Huixian Cui, Shao-guang Sun, Lei WangAbstract:Abstract Nuclear factor erythroid 2 like 2 (Nrf2) functions as a neuroprotective agent in Parkinson's disease (PD). This study aimed to investigate the key long non-coding RNAs (lncRNAs) correlated with Nrf2, which might provide valuable information for the exploration of pathogenesis of PD. The lncRNA and mRNA expression profiling of substantia nigra and Corpus Striatum of Nrf2 (-/-) mice model was obtained from microarray analysis. The animal experiments conducted for this study were approved by the ethics committee of Hebei Medical University. Bioinformatics analyses were conducted, including differentially expressed lncRNAs/mRNA (differentially expressed lncRNA, DEL/differentially expressed mRNA, DEM) identification, DEL-DEM coexpression network construction, and biological functions prediction. Quantitative real-time polymerase chain reaction (qRT-PCR) was subjected to validate abnormally expressed DELs and DEMs in the substantia nigra and Corpus Striatum of Nrf2 (-/-) mice model. A total of 48 DELs (37 down-regulated and 11 up-regulated) were identified both in Nrf2 (-/-) substantia nigra and Corpus Striatum; 96 DEMs and 643 DEMs were identified in the substantia nigra and Corpus Striatum, respectively. DEL-DEM coexpressed network was constructed. LncRNA AK076880, AK036620, and AK020330 had high connectivity with DEMs both in the substantia nigra and Corpus Striatum. These DEMs were significantly enriched in signaling pathways such as the calcium signaling pathway, Huntington's disease, Alzheimer's disease, mitogen-activated protein kinase (MAPK) signaling pathway, and the Wnt signaling pathway. Generally, qRT-PCR validation results of selected DEMs and DELs were consistent with microarray data. The dysregulated DELs and DEMs in the substantia nigra and Corpus Striatum of Nrf2 (-/-) mice were identified. Our results might provide useful information for further exploring the pathogenesis mechanism of PD.
Abdur Razzaque Siddiqui - One of the best experts on this subject based on the ideXlab platform.
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A short echo 1H spectroscopy and volumetric MRI study of the Corpus Striatum in patients with obsessive-compulsive disorder and comparison subjects
The American journal of psychiatry, 1998Co-Authors: Robert Bartha, Murray B. Stein, Peter C. Williamson, Dick J. Drost, Richard W. J. Neufeld, Thomas J. Carr, Gita Canaran, Maria Densmore, Geri Anderson, Abdur Razzaque SiddiquiAbstract:Objective:It is likely that the Corpus Striatum is involved in obsessive-compulsive disorder (OCD). Prior studies have inconsistently found alterations in caudate volumes in patients with OCD. This study was undertaken in the hope that N-acetylaspartate and volumetric measures together would elucidate the presence and nature of Corpus Striatum volumetric abnormalities in OCD. Method:Thirteen patients meeting the DSM-IV criteria for OCD, who had been medication free for a minimum of 6 weeks, and 13 psychiatrically normal matched comparison subjects participated in the study. Short echo 1H magnetic resonance spectroscopy (1H-MRS) was used to measure levels of N-acetylaspartate and several other cerebral metabolites from a 4.5-cm3 volume in the left Corpus Striatum of all 26 subjects. Metabolite levels were estimated by fitting the time domain spectroscopy data with a noninteractive computer program. Volumes of the left and right head of the caudate nucleus in each subject were determined by semiautomatic se...
