The Experts below are selected from a list of 1209 Experts worldwide ranked by ideXlab platform
Alfredo Rebora - One of the best experts on this subject based on the ideXlab platform.
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anti liver kidney microsome type 1 antibodies in lichen planus
Archives of Dermatology, 1992Co-Authors: M.c. Divano, Aurora Parodi, Alfredo ReboraAbstract:To the Editor.— The recent description of a patient with lichen planus (LP) and anti—liver-kidney microsome (LKM)— positive chronic active hepatitis 1 prompted us to verify the possible prevalence of anti-LKM type 1 (anti-LKM1) antibody in a population of patients with LP with and without chronic liver disorder. Forty-six patients with LP (25 women and 20 men) were recruited retrospectively. Sixteen had been diagnosed as having chronic liver disorder on the basis of persistent alterations in at least three of the following serum chemistry results: alanine aminotransferase, aspartate aminotransferase, γ-glutamyltransferase, alkaline phosphatase, and bilirubin. The presence of anti-LKM1 antibodies was determined by indirect immunoflourescence, using rat kidney and liver as substrates, and by Counterimmunoelectrophoresis, using rat liver as a source of antigen. 2 No anti-LKM1 antibodies were found in any patient, suggesting that their occurrence in patients with LP is only occasional. In the case reported by Cottoni and coworkers,
Ming Liu - One of the best experts on this subject based on the ideXlab platform.
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Development of an Enzyme-Linked Immunosorbent Assay Based on Fusion VP2332-452 Antigen for Detecting Antibodies against Aleutian Mink Disease Virus
Journal of clinical microbiology, 2015Co-Authors: Xiaowei Chen, Cailing Song, Yun Liu, Dafei Liu, Yun Zhang, Ming LiuAbstract:For detection of Aleutian mink disease virus (AMDV) antibodies, an enzyme-linked immunosorbent assay (ELISA) was developed using the recombinant VP2332-452 protein as an antigen. Counterimmunoelectrophoresis (CIEP) was used as a reference test to compare the results of the ELISA and Western blotting (WB); the specificity and sensitivity of the VP2332-452 ELISA were 97.9% and 97.3%, respectively, which were higher than those of WB. Therefore, this VP2332-452 ELISA may be a preferable method for detecting antibodies against AMDV.
M.c. Divano - One of the best experts on this subject based on the ideXlab platform.
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anti liver kidney microsome type 1 antibodies in lichen planus
Archives of Dermatology, 1992Co-Authors: M.c. Divano, Aurora Parodi, Alfredo ReboraAbstract:To the Editor.— The recent description of a patient with lichen planus (LP) and anti—liver-kidney microsome (LKM)— positive chronic active hepatitis 1 prompted us to verify the possible prevalence of anti-LKM type 1 (anti-LKM1) antibody in a population of patients with LP with and without chronic liver disorder. Forty-six patients with LP (25 women and 20 men) were recruited retrospectively. Sixteen had been diagnosed as having chronic liver disorder on the basis of persistent alterations in at least three of the following serum chemistry results: alanine aminotransferase, aspartate aminotransferase, γ-glutamyltransferase, alkaline phosphatase, and bilirubin. The presence of anti-LKM1 antibodies was determined by indirect immunoflourescence, using rat kidney and liver as substrates, and by Counterimmunoelectrophoresis, using rat liver as a source of antigen. 2 No anti-LKM1 antibodies were found in any patient, suggesting that their occurrence in patients with LP is only occasional. In the case reported by Cottoni and coworkers,
Xiaowei Chen - One of the best experts on this subject based on the ideXlab platform.
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Development of an Enzyme-Linked Immunosorbent Assay Based on Fusion VP2332-452 Antigen for Detecting Antibodies against Aleutian Mink Disease Virus
Journal of clinical microbiology, 2015Co-Authors: Xiaowei Chen, Cailing Song, Yun Liu, Dafei Liu, Yun Zhang, Ming LiuAbstract:For detection of Aleutian mink disease virus (AMDV) antibodies, an enzyme-linked immunosorbent assay (ELISA) was developed using the recombinant VP2332-452 protein as an antigen. Counterimmunoelectrophoresis (CIEP) was used as a reference test to compare the results of the ELISA and Western blotting (WB); the specificity and sensitivity of the VP2332-452 ELISA were 97.9% and 97.3%, respectively, which were higher than those of WB. Therefore, this VP2332-452 ELISA may be a preferable method for detecting antibodies against AMDV.
Sining Fan - One of the best experts on this subject based on the ideXlab platform.
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Development of a Peptide ELISA for the Diagnosis of Aleutian Mink Disease
PloS one, 2016Co-Authors: Lei Zhang, Yang Wang, Lu Rongguang, Xue Xianghong, Li Xintong, Mingyu Ling, Sining FanAbstract:Aleutian disease (AD) is a common immunosuppressive disease in mink farms world-wide. Since the 1980s, Counterimmunoelectrophoresis (CIEP) has been the main detection method for infection with the Aleutian Mink Disease Virus (AMDV). In this study, six peptides derived from the AMDV structural protein VP2 were designed, synthesized, and used as ELISA antigens to detect anti-AMDV antibodies in the sera of infected minks. Serum samples were collected from 764 minks in farms from five different provinces, and analyzed by both CIEP (a gold standard) and peptide ELISA. A peptide designated P1 (415 aa–433 aa) exhibited good antigenicity. A novel ELISA was developed using ovalbumin-linked peptide P1 to detect anti-AMDV antibodies in mink sera. The sensitivity and specificity of the peptide ELISA was 98.0% and 97.5%, respectively. Moreover, the ELISA also detected 342 early-stage infected samples (negative by CIEP and positive by PCR), of which 43.6% (149/342) were true positives. These results showed that the peptide ELISA had better sensitivity compared with CIEP, and therefore could be preferable over CIEP for detecting anti-AMDV antibodies in serological screening.
