The Experts below are selected from a list of 66 Experts worldwide ranked by ideXlab platform
David Taplin - One of the best experts on this subject based on the ideXlab platform.
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Isolation, amplification, and sequencing of human mitochondrial DNA obtained from human Crab Louse, Pthirus pubis (L.), blood meals.
Journal of forensic sciences, 1998Co-Authors: Wayne D. Lord, David Taplin, Bruce Budowle, Joseph A. Dizinno, Mark R. Wilson, Terri L. MeinkingAbstract:The ability to identify individual human hosts based on analyses of blood recovered from the digestive tract of hematophagous arthropods has been a long-term pursuit in both medical and forensic entomology. Blood meal individualization techniques can bring important advancements to studies of vector-borne disease epidemiology. Forensically, these analyses may aid in assailant identification in violent crime cases where blood-feeding insects or their excreta are recovered from victims or at crime scenes. Successful isolation, amplification, and sequencing of human mitochondrial DNA obtained from adult human Crab lice fed on human volunteers are reported. Adult lice were removed from recruited volunteers frequenting inner city health clinics. Live lice were killed by freezing and subsequently air dried at ambient temperature. A saliva sample was obtained from each volunteer and served as a DNA reference sample. Volunteers were afforded free, approved pediculosis treatment. Individual lice were subsequently processed using procedures developed for the extraction of mitochondrial DNA from human hair, teeth, and bone. The resulting DNA was amplified by the polymerase chain reaction and sequenced. Our results point to valuable avenues for future entomological research.
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IDENTIFICATION OF HOST DNA BY AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSIS: PRELIMINARY ANALYSIS OF HUMAN Crab Louse (ANOPLURA: PEDICULIDAE) EXCRETA
Journal of Medical Entomology, 1994Co-Authors: Jeri Replogle, Wayne D. Lord, Terri L. Meinking, Bruce Budowle, David TaplinAbstract:: The ability to identify individual hosts of hematophagous arthropods via bloodmeal analyses is a continuing pursuit in both medical and forensic entomology. Characterization of human DNA from blood-feeding arthropods has been advanced substantially by preparation techniques, such as the polymerase chain reaction (PCR). Successful application of amplified fragment length polymorphism (AMP-FLP) analysis to excreta obtained from adult Crab lice, Pthirus pubis (L.), fed on human volunteers is reported herein. Human DNA derived from Crab Louse excreta was typed successfully for two human DNA genetic markers, D1S80 and HUMTH01. Although preliminary, these results illustrate the ability of AMP-FLP analyses to provide individual human locus characterizations from desiccated arthropod excrement.
Terri L. Meinking - One of the best experts on this subject based on the ideXlab platform.
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Isolation, amplification, and sequencing of human mitochondrial DNA obtained from human Crab Louse, Pthirus pubis (L.), blood meals.
Journal of forensic sciences, 1998Co-Authors: Wayne D. Lord, David Taplin, Bruce Budowle, Joseph A. Dizinno, Mark R. Wilson, Terri L. MeinkingAbstract:The ability to identify individual human hosts based on analyses of blood recovered from the digestive tract of hematophagous arthropods has been a long-term pursuit in both medical and forensic entomology. Blood meal individualization techniques can bring important advancements to studies of vector-borne disease epidemiology. Forensically, these analyses may aid in assailant identification in violent crime cases where blood-feeding insects or their excreta are recovered from victims or at crime scenes. Successful isolation, amplification, and sequencing of human mitochondrial DNA obtained from adult human Crab lice fed on human volunteers are reported. Adult lice were removed from recruited volunteers frequenting inner city health clinics. Live lice were killed by freezing and subsequently air dried at ambient temperature. A saliva sample was obtained from each volunteer and served as a DNA reference sample. Volunteers were afforded free, approved pediculosis treatment. Individual lice were subsequently processed using procedures developed for the extraction of mitochondrial DNA from human hair, teeth, and bone. The resulting DNA was amplified by the polymerase chain reaction and sequenced. Our results point to valuable avenues for future entomological research.
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IDENTIFICATION OF HOST DNA BY AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSIS: PRELIMINARY ANALYSIS OF HUMAN Crab Louse (ANOPLURA: PEDICULIDAE) EXCRETA
Journal of Medical Entomology, 1994Co-Authors: Jeri Replogle, Wayne D. Lord, Terri L. Meinking, Bruce Budowle, David TaplinAbstract:: The ability to identify individual hosts of hematophagous arthropods via bloodmeal analyses is a continuing pursuit in both medical and forensic entomology. Characterization of human DNA from blood-feeding arthropods has been advanced substantially by preparation techniques, such as the polymerase chain reaction (PCR). Successful application of amplified fragment length polymorphism (AMP-FLP) analysis to excreta obtained from adult Crab lice, Pthirus pubis (L.), fed on human volunteers is reported herein. Human DNA derived from Crab Louse excreta was typed successfully for two human DNA genetic markers, D1S80 and HUMTH01. Although preliminary, these results illustrate the ability of AMP-FLP analyses to provide individual human locus characterizations from desiccated arthropod excrement.
Wayne D. Lord - One of the best experts on this subject based on the ideXlab platform.
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Isolation, amplification, and sequencing of human mitochondrial DNA obtained from human Crab Louse, Pthirus pubis (L.), blood meals.
Journal of forensic sciences, 1998Co-Authors: Wayne D. Lord, David Taplin, Bruce Budowle, Joseph A. Dizinno, Mark R. Wilson, Terri L. MeinkingAbstract:The ability to identify individual human hosts based on analyses of blood recovered from the digestive tract of hematophagous arthropods has been a long-term pursuit in both medical and forensic entomology. Blood meal individualization techniques can bring important advancements to studies of vector-borne disease epidemiology. Forensically, these analyses may aid in assailant identification in violent crime cases where blood-feeding insects or their excreta are recovered from victims or at crime scenes. Successful isolation, amplification, and sequencing of human mitochondrial DNA obtained from adult human Crab lice fed on human volunteers are reported. Adult lice were removed from recruited volunteers frequenting inner city health clinics. Live lice were killed by freezing and subsequently air dried at ambient temperature. A saliva sample was obtained from each volunteer and served as a DNA reference sample. Volunteers were afforded free, approved pediculosis treatment. Individual lice were subsequently processed using procedures developed for the extraction of mitochondrial DNA from human hair, teeth, and bone. The resulting DNA was amplified by the polymerase chain reaction and sequenced. Our results point to valuable avenues for future entomological research.
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IDENTIFICATION OF HOST DNA BY AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSIS: PRELIMINARY ANALYSIS OF HUMAN Crab Louse (ANOPLURA: PEDICULIDAE) EXCRETA
Journal of Medical Entomology, 1994Co-Authors: Jeri Replogle, Wayne D. Lord, Terri L. Meinking, Bruce Budowle, David TaplinAbstract:: The ability to identify individual hosts of hematophagous arthropods via bloodmeal analyses is a continuing pursuit in both medical and forensic entomology. Characterization of human DNA from blood-feeding arthropods has been advanced substantially by preparation techniques, such as the polymerase chain reaction (PCR). Successful application of amplified fragment length polymorphism (AMP-FLP) analysis to excreta obtained from adult Crab lice, Pthirus pubis (L.), fed on human volunteers is reported herein. Human DNA derived from Crab Louse excreta was typed successfully for two human DNA genetic markers, D1S80 and HUMTH01. Although preliminary, these results illustrate the ability of AMP-FLP analyses to provide individual human locus characterizations from desiccated arthropod excrement.
Bruce Budowle - One of the best experts on this subject based on the ideXlab platform.
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Isolation, amplification, and sequencing of human mitochondrial DNA obtained from human Crab Louse, Pthirus pubis (L.), blood meals.
Journal of forensic sciences, 1998Co-Authors: Wayne D. Lord, David Taplin, Bruce Budowle, Joseph A. Dizinno, Mark R. Wilson, Terri L. MeinkingAbstract:The ability to identify individual human hosts based on analyses of blood recovered from the digestive tract of hematophagous arthropods has been a long-term pursuit in both medical and forensic entomology. Blood meal individualization techniques can bring important advancements to studies of vector-borne disease epidemiology. Forensically, these analyses may aid in assailant identification in violent crime cases where blood-feeding insects or their excreta are recovered from victims or at crime scenes. Successful isolation, amplification, and sequencing of human mitochondrial DNA obtained from adult human Crab lice fed on human volunteers are reported. Adult lice were removed from recruited volunteers frequenting inner city health clinics. Live lice were killed by freezing and subsequently air dried at ambient temperature. A saliva sample was obtained from each volunteer and served as a DNA reference sample. Volunteers were afforded free, approved pediculosis treatment. Individual lice were subsequently processed using procedures developed for the extraction of mitochondrial DNA from human hair, teeth, and bone. The resulting DNA was amplified by the polymerase chain reaction and sequenced. Our results point to valuable avenues for future entomological research.
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IDENTIFICATION OF HOST DNA BY AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSIS: PRELIMINARY ANALYSIS OF HUMAN Crab Louse (ANOPLURA: PEDICULIDAE) EXCRETA
Journal of Medical Entomology, 1994Co-Authors: Jeri Replogle, Wayne D. Lord, Terri L. Meinking, Bruce Budowle, David TaplinAbstract:: The ability to identify individual hosts of hematophagous arthropods via bloodmeal analyses is a continuing pursuit in both medical and forensic entomology. Characterization of human DNA from blood-feeding arthropods has been advanced substantially by preparation techniques, such as the polymerase chain reaction (PCR). Successful application of amplified fragment length polymorphism (AMP-FLP) analysis to excreta obtained from adult Crab lice, Pthirus pubis (L.), fed on human volunteers is reported herein. Human DNA derived from Crab Louse excreta was typed successfully for two human DNA genetic markers, D1S80 and HUMTH01. Although preliminary, these results illustrate the ability of AMP-FLP analyses to provide individual human locus characterizations from desiccated arthropod excrement.
Jeri Replogle - One of the best experts on this subject based on the ideXlab platform.
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IDENTIFICATION OF HOST DNA BY AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSIS: PRELIMINARY ANALYSIS OF HUMAN Crab Louse (ANOPLURA: PEDICULIDAE) EXCRETA
Journal of Medical Entomology, 1994Co-Authors: Jeri Replogle, Wayne D. Lord, Terri L. Meinking, Bruce Budowle, David TaplinAbstract:: The ability to identify individual hosts of hematophagous arthropods via bloodmeal analyses is a continuing pursuit in both medical and forensic entomology. Characterization of human DNA from blood-feeding arthropods has been advanced substantially by preparation techniques, such as the polymerase chain reaction (PCR). Successful application of amplified fragment length polymorphism (AMP-FLP) analysis to excreta obtained from adult Crab lice, Pthirus pubis (L.), fed on human volunteers is reported herein. Human DNA derived from Crab Louse excreta was typed successfully for two human DNA genetic markers, D1S80 and HUMTH01. Although preliminary, these results illustrate the ability of AMP-FLP analyses to provide individual human locus characterizations from desiccated arthropod excrement.
