The Experts below are selected from a list of 165 Experts worldwide ranked by ideXlab platform

Sharonda Q. Bradley - One of the best experts on this subject based on the ideXlab platform.

  • Diepoxybutane interstrand cross-links induce DNA bending
    Biochimie, 2011
    Co-Authors: Julie T. Millard, Erin E. Mcgowan, Sharonda Q. Bradley
    Abstract:

    The bifunctional alkylating agent 1,2,3,4-diepoxybutane (DEB) is thought to be a major contributor to the carcinogenicity of 1,3-butadiene, from which it is derived in vivo. DEB forms DNA interstrand cross-links primarily between distal deoxyguanosine residues at the duplex sequence 5'-GNC. In order for the short butanediol tether to span this distance, distortion of the DNA target has been postulated. We determined that the electrophoretic mobility of ligated DNA oligomers containing DEB cross-links was retarded in comparison with control, uncross-linked DNA. Our data are consistent with DNA bending of ∼34° per lesion towards the major groove.

Andrew Deeks - One of the best experts on this subject based on the ideXlab platform.

  • Ultimate bending capacity evaluation of laminated bamboo lumber beams
    Construction and Building Materials, 2018
    Co-Authors: Qisheng Zhang, Andrew Deeks
    Abstract:

    Abstract In order to evaluate the bending capacity of laminated bamboo lumber (LBL) beams, material performance tests and beam tests have been carried out. A strain-stress relationship is proposed based on compression and tensile tests conducted parallel to the grain. Two failure modes are identified based on the locations of cracks in the beam specimens. Direction of bending and the location of internal joints are shown to influence the mechanical properties of LBL beams. Internal joints have more influence on specimens under tangential bending (where the culms are bent across their width) than that under radial bending (where the culms are bent across their thickness). Irrespective of the bending direction, the strain across the cross-section of the laminated bamboo beam is shown to be linear throughout the test process, following standard beam theory. Based on this theory and the proposed simplified strain–stress relationship for the beam, calculation approaches for the ultimate bending moment and ultimate bending deflection are proposed for three bending failure modes which give a good agreement with the test results.

Ruth N. Zadoks - One of the best experts on this subject based on the ideXlab platform.

  • Fecal shedding of Klebsiella pneumoniae by dairy cows.
    Journal of dairy science, 2006
    Co-Authors: M.a. Munoz, C. Ahlström, B.j. Rauch, Ruth N. Zadoks
    Abstract:

    Klebsiella pneumoniae is a common cause of clinical mastitis in dairy cattle. Wood products are considered to be the main source of Klebsiella on dairy farms. Environmental hygiene and use of inorganic bedding materials such as sand are recommended to control Klebsiella mastitis. However, Klebsiella mastitis still occurs on well-managed dairy farms that use sand as bedding material. In a 5-mo study in a New York State dairy herd performed during the summer of 2005, all of 9 samples of unused sand bedding tested negative for Klebsiella, whereas 14 of 18 samples of used sand bedding contained Klebsiella at a median level of 10(4.6) cfu/g. We hypothesized that fecal shedding of Klebsiella by dairy cows contributes to the presence of Klebsiella in the environment. Using a cheap and simple method based on ampicillin-containing MacConkey agar for screening, and biochemical tests for confirmation of species identity, 595 fecal samples from healthy dairy cattle were screened for presence of Klebsiella. In a longitudinal study of 100 cows followed over 5 mo, more than 80% of fecal samples tested positive for K. pneumoniae. The average prevalence of K. pneumoniae-positive fecal samples was also above 80% in a cross-sectional study of 100 cows from 10 herds across New York and Massachusetts. Fecal shedding of K. pneumoniae by a large proportion of dairy cows may explain why Klebsiella mastitis occurs in herds that use inorganic bedding material or other bedding material that is free from Klebsiella upon introduction into the barn.

Didier Marquis - One of the best experts on this subject based on the ideXlab platform.

  • Effect of a machining-induced defect on the tensile strength of a 3D composite material
    Journal of Materials Science, 2003
    Co-Authors: Catherine A. Davy, Didier Marquis
    Abstract:

    This paper investigates the effect of finite specimen size upon the tensile failure of a tridirectional carbon-carbon composite along each reinforcement axis. Asymmetry in the position of load-bearing axial yarns across the cross-section is generated randomly by machining. This yields parasitic bending of the specimen, and thereby premature failure of the yarns subjected to the maximum bending stress. However, bending effects become negligible at final failure. Additionally, the composite failure strength σ F is determined from the cross-sectional area of the actually load-bearing axial yarns, using both symmetrical and asymmetrical specimens. Results are in good agreement with previous work, and we show that the variability of σ F is small.

Mary Oyle - One of the best experts on this subject based on the ideXlab platform.

  • cross sectional study of the relationships among bedding materials bedding bacteria counts and intramammary infection in late lactation dairy cows
    Journal of Dairy Science, 2019
    Co-Authors: S M Rowe, Eri E Royste, J Timmerma, Almudena Crooke, Sandra Godde, Mary Oyle
    Abstract:

    ABSTRACT Objectives of this study were to (1) describe the intramammary infection (IMI) prevalence and pathogen profiles in quarters of cows approaching dry-off in US dairy herds, (2) compare IMI prevalence in quarters of cows exposed to different bedding material types, and (3) identify associations between bedding bacteria count and IMI in cows approaching dry-off. Eighty herds using 1 of 4 common bedding materials (manure solids, organic non-manure, new sand, and recycled sand) were recruited in a multi-site cross-sectional study. Each herd was visited twice for sampling. At each visit, aseptic quarter-milk samples were collected from 20 cows approaching dry-off (>180 d pregnant). Samples of unused and used bedding were also collected. Aerobic culture was used to determine the IMI status of 10,448 quarters and to enumerate counts (log10 cfu/mL) of all bacteria, Staphylococcus spp., Streptococcus spp. and Streptococcus-like organisms (SSLO), coliforms, Klebsiella spp., noncoliform gram-negatives, Bacillus spp., and Prototheca spp. in unused (n = 148) and used (n = 150) bedding. The association between bedding bacteria count and IMI was determined using multivariable logistic regression with mixed effects. Quarter-level prevalence of IMI was 21.1%, which was primarily caused by non-aureus Staphylococcus spp. (11.4%) and SSLO (5.6%). Only modest differences in IMI prevalence were observed between the 4 common bedding material types. Counts of all bacteria in unused bedding was positively associated with odds of IMI caused by any pathogen [ALL-IMI; odds ratio (OR) = 1.08]. A positive association was also observed for counts of SSLO in unused bedding and SSLO-IMI (OR = 1.09). These patterns of association were generally consistent across the 4 common bedding materials. In contrast, the association between counts of all bacteria in used bedding and ALL-IMI varied by bedding type, with positive associations observed in quarters exposed to manure solids (OR = 2.29) and organic non-manure (OR = 1.51) and a negative association in quarters exposed to new sand (OR = 0.47). Findings from this study suggest that quarter-level IMI prevalence in late-lactation cows is low in US dairy herds. Furthermore, bedding material type may not be an important risk factor for IMI in late lactation. Higher levels of bacteria in bedding may increase IMI prevalence at dry-off in general, but this relationship is likely to vary according to bedding material type.