The Experts below are selected from a list of 117 Experts worldwide ranked by ideXlab platform
Christopher R. Bellenger - One of the best experts on this subject based on the ideXlab platform.
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Ventricular septal defect repair in a small dog using Cross-Circulation.
Australian veterinary journal, 1995Co-Authors: Geraldine B Hunt, M. R B Pearson, Christopher R. Bellenger, Richard MalikAbstract:SUMMARY A haemodynamically significant ventricular septal defect was diagnosed in a 3-month-old male Cavalier King Charles Spaniel. A median sternotomy was performed and the 6.5 kg dog placed on cardiopulmonary bypass using pump-assisted Cross-Circulation. A 10 mm diameter peri-membranous ventricular septal defect was closed using a continuous suture of 4–0 polypropylene, via a 2.5 cm incision in the right ventricular outflow tract. The duration of cardiopulmonary bypass was 90 minutes. Complications in the immediate postoperative period were mild and easily managed.
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An experimental study of Cross-Circulation for cardiopulmonary bypass in dogs
Research in veterinary science, 1993Co-Authors: Geraldine B Hunt, M. R B Pearson, Christopher R. BellengerAbstract:Cardiopulmonary bypass using Cross-Circulation was evaluated in dogs. In eight experiments a greyhound (the donor) was used to perfuse a small mongrel dog (the recipient). Recipients averaged 40 per cent of the donor's weight. Total cardiopulmonary bypass was successfully established in all eight experiments and maintained for 60 to 90 minutes. Haemodynamic values (mean arterial pressure, central venous pressure, cardiac output) and arterial blood gas tensions in the recipient and donor dogs remained at near-baseline levels for the duration of cardiopulmonary bypass. Seven of the eight recipient dogs were successfully weaned from cardiopulmonary bypass at the end of the procedure.
Geraldine B Hunt - One of the best experts on this subject based on the ideXlab platform.
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Ventricular septal defect repair in a small dog using Cross-Circulation.
Australian veterinary journal, 1995Co-Authors: Geraldine B Hunt, M. R B Pearson, Christopher R. Bellenger, Richard MalikAbstract:SUMMARY A haemodynamically significant ventricular septal defect was diagnosed in a 3-month-old male Cavalier King Charles Spaniel. A median sternotomy was performed and the 6.5 kg dog placed on cardiopulmonary bypass using pump-assisted Cross-Circulation. A 10 mm diameter peri-membranous ventricular septal defect was closed using a continuous suture of 4–0 polypropylene, via a 2.5 cm incision in the right ventricular outflow tract. The duration of cardiopulmonary bypass was 90 minutes. Complications in the immediate postoperative period were mild and easily managed.
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An experimental study of Cross-Circulation for cardiopulmonary bypass in dogs
Research in veterinary science, 1993Co-Authors: Geraldine B Hunt, M. R B Pearson, Christopher R. BellengerAbstract:Cardiopulmonary bypass using Cross-Circulation was evaluated in dogs. In eight experiments a greyhound (the donor) was used to perfuse a small mongrel dog (the recipient). Recipients averaged 40 per cent of the donor's weight. Total cardiopulmonary bypass was successfully established in all eight experiments and maintained for 60 to 90 minutes. Haemodynamic values (mean arterial pressure, central venous pressure, cardiac output) and arterial blood gas tensions in the recipient and donor dogs remained at near-baseline levels for the duration of cardiopulmonary bypass. Seven of the eight recipient dogs were successfully weaned from cardiopulmonary bypass at the end of the procedure.
M. R B Pearson - One of the best experts on this subject based on the ideXlab platform.
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Ventricular septal defect repair in a small dog using Cross-Circulation.
Australian veterinary journal, 1995Co-Authors: Geraldine B Hunt, M. R B Pearson, Christopher R. Bellenger, Richard MalikAbstract:SUMMARY A haemodynamically significant ventricular septal defect was diagnosed in a 3-month-old male Cavalier King Charles Spaniel. A median sternotomy was performed and the 6.5 kg dog placed on cardiopulmonary bypass using pump-assisted Cross-Circulation. A 10 mm diameter peri-membranous ventricular septal defect was closed using a continuous suture of 4–0 polypropylene, via a 2.5 cm incision in the right ventricular outflow tract. The duration of cardiopulmonary bypass was 90 minutes. Complications in the immediate postoperative period were mild and easily managed.
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An experimental study of Cross-Circulation for cardiopulmonary bypass in dogs
Research in veterinary science, 1993Co-Authors: Geraldine B Hunt, M. R B Pearson, Christopher R. BellengerAbstract:Cardiopulmonary bypass using Cross-Circulation was evaluated in dogs. In eight experiments a greyhound (the donor) was used to perfuse a small mongrel dog (the recipient). Recipients averaged 40 per cent of the donor's weight. Total cardiopulmonary bypass was successfully established in all eight experiments and maintained for 60 to 90 minutes. Haemodynamic values (mean arterial pressure, central venous pressure, cardiac output) and arterial blood gas tensions in the recipient and donor dogs remained at near-baseline levels for the duration of cardiopulmonary bypass. Seven of the eight recipient dogs were successfully weaned from cardiopulmonary bypass at the end of the procedure.
John D. O’neill - One of the best experts on this subject based on the ideXlab platform.
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Xenogeneic Cross-Circulation for extracorporeal recovery of injured human lungs.
Nature medicine, 2020Co-Authors: Ahmed E. Hozain, John D. O’neill, Kenmond Fung, Rachel Donocoff, Yuliya Tipograf, Meghan R. Pinezich, Katherine M. Cunningham, Andrew Tumen, Rei Ukita, Michael SimpsonAbstract:Patients awaiting lung transplantation face high wait-list mortality, as injury precludes the use of most donor lungs. Although ex vivo lung perfusion (EVLP) is able to recover marginal quality donor lungs, extension of normothermic support beyond 6 h has been challenging. Here we demonstrate that acutely injured human lungs declined for transplantation, including a lung that failed to recover on EVLP, can be recovered by Cross-Circulation of whole blood between explanted human lungs and a Yorkshire swine. This xenogeneic platform provided explanted human lungs a supportive, physiologic milieu and systemic regulation that resulted in functional and histological recovery after 24 h of normothermic support. Our findings suggest that Cross-Circulation can serve as a complementary approach to clinical EVLP to recover injured donor lungs that could not otherwise be utilized for transplantation, as well as a translational research platform for immunomodulation and advanced organ bioengineering.
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Xenogeneic Cross-Circulation for extracorporeal recovery of injured human lungs
Nature Medicine, 2020Co-Authors: Ahmed E. Hozain, John D. O’neill, Kenmond Fung, Rachel Donocoff, Yuliya Tipograf, Meghan R. Pinezich, Katherine M. Cunningham, Andrew Tumen, Rei Ukita, Michael T. SimpsonAbstract:In a new strategy for increasing the availability of lungs for transplantation, human lungs declined for transplantation because of their poor quality can be recuperated by connecting them to the Circulation of a pig. Patients awaiting lung transplantation face high wait-list mortality, as injury precludes the use of most donor lungs. Although ex vivo lung perfusion (EVLP) is able to recover marginal quality donor lungs, extension of normothermic support beyond 6 h has been challenging. Here we demonstrate that acutely injured human lungs declined for transplantation, including a lung that failed to recover on EVLP, can be recovered by Cross-Circulation of whole blood between explanted human lungs and a Yorkshire swine. This xenogeneic platform provided explanted human lungs a supportive, physiologic milieu and systemic regulation that resulted in functional and histological recovery after 24 h of normothermic support. Our findings suggest that Cross-Circulation can serve as a complementary approach to clinical EVLP to recover injured donor lungs that could not otherwise be utilized for transplantation, as well as a translational research platform for immunomodulation and advanced organ bioengineering.
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Multiday maintenance of extracorporeal lungs using Cross-Circulation with conscious swine.
The Journal of thoracic and cardiovascular surgery, 2019Co-Authors: Ahmed E. Hozain, Brandon A. Guenthart, Dawn Queen, Kenmond Fung, Rachel Donocoff, Charles C. Marboe, Yuliya Tipograf, Meghan R. Pinezich, Katherine M. Cunningham, John D. O’neillAbstract:Abstract Objectives Lung remains the least-utilized solid organ for transplantation. Efforts to recover donor lungs with reversible injuries using ex vivo perfusion systems are limited to Methods A swine behavioral training program and custom enclosure were developed to enable multiday Cross-Circulation between extracorporeal lungs and recipient swine. Lungs were ventilated and perfused in a normothermic chamber for 4 days. Longitudinal analyses of extracorporeal lungs (ie, functional assessments, multiscale imaging, cytokine quantification, and cellular assays) and recipient swine (eg, vital signs and blood and tissue analyses) were performed. Results Throughout 4 days of normothermic support, extracorporeal lung function was maintained (arterial oxygen tension/inspired oxygen fraction >400 mm Hg; compliance >20 mL/cm H2O), and recipient swine were hemodynamically stable (lactate Conclusions We demonstrate feasibility of normothermic maintenance of extracorporeal lungs for 4 days by Cross-Circulation with conscious swine. Cross-Circulation approaches could support the recovery of damaged lungs and enable organ bioengineering to improve transplant outcomes.
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Regeneration of severely damaged lungs using an interventional Cross-Circulation platform.
Nature communications, 2019Co-Authors: Brandon A. Guenthart, John D. O’neill, Jinho Kim, Scott Chicotka, Dawn Queen, Kenmond Fung, Michael Simpson, Rachel Donocoff, Michael Salna, Charles C. MarboeAbstract:The number of available donor organs limits lung transplantation, the only lifesaving therapy for the increasing population of patients with end-stage lung disease. A prevalent etiology of injury that renders lungs unacceptable for transplantation is gastric aspiration, a deleterious insult to the pulmonary epithelium. Currently, severely damaged donor lungs cannot be salvaged with existing devices or methods. Here we report the regeneration of severely damaged lungs repaired to meet transplantation criteria by utilizing an interventional Cross-Circulation platform in a clinically relevant swine model of gastric aspiration injury. Enabled by Cross-Circulation with a living swine, prolonged extracorporeal support of damaged lungs results in significant improvements in lung function, cellular regeneration, and the development of diagnostic tools for non-invasive organ evaluation and repair. We therefore propose that the use of an interventional Cross-Circulation platform could enable recovery of otherwise unsalvageable lungs and thus expand the donor organ pool.
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Cross-Circulation for extracorporeal support and recovery of the lung
Nature Biomedical Engineering, 2017Co-Authors: John D. O’neill, Brandon A. Guenthart, Jinho Kim, Scott Chicotka, Dawn Queen, Kenmond Fung, Charles Marboe, Alexander Romanov, Sarah X. L. Huang, Ya-wen ChenAbstract:The shortage of transplantable donor organs has profound consequences, especially for patients with end-stage lung disease, for which transplantation remains the only definitive treatment. Although advances in ex vivo lung perfusion have enabled the evaluation and reconditioning of marginally unacceptable donor lungs, clinical use of the technique is limited to ~6 h. Extending the duration of extracorporeal organ support from hours to days would enable longer recovery and recipient-specific manipulations of the donor lung, with the goal of expanding the donor organ pool and improving long-term outcomes. By using a clinically relevant swine model, here we report the development of a Cross-Circulation platform wherein recipient support enabled 36 h of normothermic perfusion that maintained healthy lungs and allowed for the recovery of injured lungs. Extended support enabled multiscale therapeutic interventions in all extracorporeal lungs. Lungs exceeded transplantation criteria, and recipients tolerated Cross-Circulation with no significant changes in physiologic parameters throughout 36 h of support. Our findings suggest that Cross-Circulation should enable extended support and interventions in extracorporeal organs. An extracorporeal Cross-Circulation approach enables, in a swine model, 36 hours of normothermic perfusion in healthy lungs, the recovery of injured lungs, and extended therapeutic interventions in all lungs.
Steven P. Keller - One of the best experts on this subject based on the ideXlab platform.
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Living swine to maintain donor organs
Science Translational Medicine, 2020Co-Authors: Steven P. KellerAbstract:Use of xenogeneic Cross-Circulation recovered injured human lungs and expand the pool of donor organs in lung transplantation.