The Experts below are selected from a list of 77418 Experts worldwide ranked by ideXlab platform
Qiaochun Wang - One of the best experts on this subject based on the ideXlab platform.
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advances in Cryopreservation of in vitro derived propagules technologies and explant sources
Plant Cell Tissue and Organ Culture, 2020Co-Authors: Minrui Wang, Florent Engelmann, Maurizio Lambardi, Ranjith Pathirana, Bart Panis, Gayle M Volk, Qiaochun WangAbstract:Genetic improvements in plant breeding are dependent upon having access to novel plant genetic resources that are available in plant genebanks. Many crops that are vegetatively-propagated are maintained as plants in the field or greenhouse, making them vulnerable to biotic and abiotic threats. Increasingly, plant genebanks are using Cryopreservation technologies to secure vegetatively propagated collections at secondary locations. Droplet vitrification and cryo-plate Cryopreservation methods have been used to successfully cryopreserve the shoot tips of many plant species. New propagule types, including small leaf square-bearing adventitious buds, stem disc-bearing adventitious buds, microtubers and rhizome buds are alternative explants for use in Cryopreservation. This review describes new technologies for in-vitro based Cryopreservation systems that have advanced the field of plant Cryopreservation. Future advances will allow even more diverse germplasm to be successfully preserved in cryobanks. New technologies for in-vitro based Cryopreservation systems have advanced the field of plant Cryopreservation since the twenty first century. Further advances will certainly facilitate even more diverse germplasm to be successfully preserved in cryobanks.
Dirk Wedekind - One of the best experts on this subject based on the ideXlab platform.
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Cryopreservation and orthotopic transplantation of rat ovaries.
Methods in molecular biology (Clifton N.J.), 2009Co-Authors: Martina Dorsch, Dirk WedekindAbstract:The number of rat strains increased considerably in the last decade and will increase continuously during the next years. This requires enough space for maintaining vital strains and techniques for cryobanking, which can be applied not only in specialised rat resource centres but also in regular animal houses. Here we describe an easy and fast method for the Cryopreservation and transplantation of frozen-thawed ovaries of the rat. With dimethyl sulfoxide as cryoprotectant rat ovaries can be stored at -196 degrees C for unlimited time. For revitalisation thawed ovaries have to be orthotopically transplanted into appropriate ovarectomised recipients. Reestablishment of the reproductive cycle in the recipients can be confirmed by vaginal cytology shortly after transplantation. The recipients are able to produce 2-3 litters after mating with males of an appropriate strain. Cyropreservation of ovaries thus can be considered a reliable method to preserve scientifically and economically important stocks and strains of rats that are currently not required.
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Cryopreservation and orthotopic transplantation of rat ovaries as a means of gamete banking
Laboratory animals, 2007Co-Authors: Martina Dorsch, Dirk Wedekind, Kenji Kamino, Hans J. HedrichAbstract:Summary Besides the exponentially increasing number of mouse strains, the rising number of rat strains, due to the establishment of transgenic and coisogenic strains in this species, surpasses the capacity of most animal houses. Cryopreservation of gametes may be a means of solving these problems. Here we describe an easy and fast method for the Cryopreservation and transplantation of frozen–thawed ovaries of the rat. Ovaries of the rat inbred strain WKY/ Ztm were frozen with dimethylsulphoxide as cryoprotectant and stored at � 1961C. Orthotopical transplantation was performed into ovariectomized syngenic recipients. Re-establishment of the reproductive cycle in the recipients was confirmed by vaginal cytology. The morphological integrity of frozen and unfrozen ovaries was compared by histological means after staining with haematoxylin and eosin. The number of litters and offspring was recorded. Reproductive cycle was re-established in all recipients of unfrozen ovaries and in more than 50% of recipients that received frozen–thawed ovaries. One-third of the former and more than 25% of the latter became pregnant and delivered at least one litter. Cyropreservation of ovaries can thus be considered as a reliable method of preserving scientifically and economically important mutant stock, as well as congenic rat strains that are currently not required.
Bart Panis - One of the best experts on this subject based on the ideXlab platform.
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advances in Cryopreservation of in vitro derived propagules technologies and explant sources
Plant Cell Tissue and Organ Culture, 2020Co-Authors: Minrui Wang, Florent Engelmann, Maurizio Lambardi, Ranjith Pathirana, Bart Panis, Gayle M Volk, Qiaochun WangAbstract:Genetic improvements in plant breeding are dependent upon having access to novel plant genetic resources that are available in plant genebanks. Many crops that are vegetatively-propagated are maintained as plants in the field or greenhouse, making them vulnerable to biotic and abiotic threats. Increasingly, plant genebanks are using Cryopreservation technologies to secure vegetatively propagated collections at secondary locations. Droplet vitrification and cryo-plate Cryopreservation methods have been used to successfully cryopreserve the shoot tips of many plant species. New propagule types, including small leaf square-bearing adventitious buds, stem disc-bearing adventitious buds, microtubers and rhizome buds are alternative explants for use in Cryopreservation. This review describes new technologies for in-vitro based Cryopreservation systems that have advanced the field of plant Cryopreservation. Future advances will allow even more diverse germplasm to be successfully preserved in cryobanks. New technologies for in-vitro based Cryopreservation systems have advanced the field of plant Cryopreservation since the twenty first century. Further advances will certainly facilitate even more diverse germplasm to be successfully preserved in cryobanks.
Hiroshi Okada - One of the best experts on this subject based on the ideXlab platform.
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A questionnaire survey on attitude toward sperm Cryopreservation among hematologists in Japan.
International Journal of Hematology, 2016Co-Authors: Tomohiro Kobayashi, Takeshi Shin, Kojiro Nishio, Yukihito Shimomura, Toshiyuki Iwahata, Keisuke Suzuki, Akane Miyata, Yoshitomo Kobori, Gaku Arai, Hiroshi OkadaAbstract:Advances in multimodal treatment have led to dramatic improvement in cancer treatment outcomes. It is now necessary to consider cancer patients’ holistic quality of life. Fertility preservation is the top concern for cancer survivors of reproductive age. Sperm Cryopreservation before treatment is recommended for postpubescent men, but many patients lose fertility without having been informed about options for fertility preservation. To determine how sperm Cryopreservation is perceived and practiced in Japan, we surveyed hematologists who often treat young males. A questionnaire about sperm Cryopreservation was sent to 45 major hematology institutions. A total of 22 institutions responded before the deadline. All institutions but one responded that they felt sperm Cryopreservation is necessary. Only 15 institutions responded that they inform patients about sperm Cryopreservation, and 12 institutions responded that they perform sperm Cryopreservation before chemotherapy. A total of 213 young males started their first course of chemotherapy during the survey period, of whom 61 (28.6%) had their sperm cryopreserved. Although almost all hematologists stated that sperm Cryopreservation is necessary for fertility preservation, not all institutions informed patients about it. Our findings indicate that, to promote fertility preservation in Japan, it will be necessary to systematize sperm Cryopreservation and build inter-hospital networks.
Gayle M Volk - One of the best experts on this subject based on the ideXlab platform.
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Challenges in implementing plant shoot tip Cryopreservation technologies
Plant Cell Tissue and Organ Culture (PCTOC), 2020Co-Authors: Jean Carlos Bettoni, Remi Bonnart, Gayle M VolkAbstract:Cryopreservation projects must have clear goals, long-term funding, skilled technical support staff, necessary infrastructure, in addition to well-defined procedures and protocols. All of these factors must be considered and optimized prior to the implementation of a Cryopreservation procedure. Cryopreservation is a safe approach for the long-term preservation of plant genetic resources that complements traditional field and in vitro germplasm collections. There are many reports of Cryopreservation successes in the literature; however, only a fraction of those methods has been routinely used for long-term conservation. A common problem is the difficulty of transferring technology and validating protocols from one laboratory to another. There are many critical factors involved including: the quality and type of plant materials, pretreatment and preculture conditions, Cryopreservation method, cooling, storage and warming conditions, and regrowth assessment methods. In addition, adequate facilities and trained personnel are needed. Herein, we present practical aspects with regard to the challenges in implementing plant shoot tip Cryopreservation technologies in cryo-banks.
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advances in Cryopreservation of in vitro derived propagules technologies and explant sources
Plant Cell Tissue and Organ Culture, 2020Co-Authors: Minrui Wang, Florent Engelmann, Maurizio Lambardi, Ranjith Pathirana, Bart Panis, Gayle M Volk, Qiaochun WangAbstract:Genetic improvements in plant breeding are dependent upon having access to novel plant genetic resources that are available in plant genebanks. Many crops that are vegetatively-propagated are maintained as plants in the field or greenhouse, making them vulnerable to biotic and abiotic threats. Increasingly, plant genebanks are using Cryopreservation technologies to secure vegetatively propagated collections at secondary locations. Droplet vitrification and cryo-plate Cryopreservation methods have been used to successfully cryopreserve the shoot tips of many plant species. New propagule types, including small leaf square-bearing adventitious buds, stem disc-bearing adventitious buds, microtubers and rhizome buds are alternative explants for use in Cryopreservation. This review describes new technologies for in-vitro based Cryopreservation systems that have advanced the field of plant Cryopreservation. Future advances will allow even more diverse germplasm to be successfully preserved in cryobanks. New technologies for in-vitro based Cryopreservation systems have advanced the field of plant Cryopreservation since the twenty first century. Further advances will certainly facilitate even more diverse germplasm to be successfully preserved in cryobanks.