The Experts below are selected from a list of 207 Experts worldwide ranked by ideXlab platform

Marilyn J. Roossinck - One of the best experts on this subject based on the ideXlab platform.

  • Cucumber Mosaic Virus
    2018
    Co-Authors: Peter Palukaitis, Marilyn J. Roossinck, Ralf G Dietzgen, R. I. B. Francki
    Abstract:

    Publisher Summary Cucumber Mosaic Virus (CMV), the type member of the cucumoVirus group, was first reported in 1916 as the causal agent of a disease of Cucumber and muskmelon in Michigan and Cucumber in New York. Since then, CMV has been found in most countries of the world, predominantly in the temperate zones, but increasingly more often in the tropical countries. CMV has the largest host range of any Virus. The number of plant species identified as hosts for CMV has increased steadily over the past 60 years. The highlights of the more recent research include the following: (1) the complete nucleotide sequence of the genome of three strains of CMV has been determined, as well as nucleotide sequences of individual RNAs of eight other CMV strains, (2) the CMV replicase has been purified to homogeneity, and it functions in vitro to synthesize CMV RNA de novo , (3) infectious transcripts have been synthesized from full-length cDNA clones of the three strains of CMV, (4) these biologically active cDNAs are being used to map sequences involved in replication, movement, pathogenesis, resistance, and vector transmission. Biologically active cDNA clones of the satellite RNAs of CMV have been produced in seven laboratories and sequences involved in replication and pathogenicity have/are being identified, (5) finally, transgenic plants have been produced expressing either the CMV coat protein gene or satellite RNA sequences that show to protect such plants from infection by CMV. This chapter, while focusing on the more recent developments in CMV biology and biochemistry, also covers some of the same ground albeit in brief. The chapter presents a comprehensive review that can be used as a reference work for general virologists and plant pathologists, as well as those specializing in the molecular biology of CMV and/or other multicomponent plant Viruses.

  • Fixation of Emerging Interviral Recombinants in Cucumber Mosaic Virus Populations
    Journal of virology, 2012
    Co-Authors: Justin S. Pita, Marilyn J. Roossinck
    Abstract:

    Interstrain recombinants were observed in the progenies of the Cucumber Mosaic Virus (CMV) reassortant L1L2F3 containing RNAs 1 and 2 from LS-CMV and RNA 3 from Fny-CMV. We characterized these recombinants, and we found that their fixation was controlled by the nature of the replicating RNAs 1 and 2. We demonstrate that the 2b gene partially affects this fixation process, but only in the context of homologous RNAs 1 and 2.

  • Core promoter for initiation of Cucumber Mosaic Virus subgenomic RNA4A.
    Molecular plant pathology, 2002
    Co-Authors: K. Sivakumaran, Marilyn J. Roossinck, M.-h. Chen, C. Cheng Kao
    Abstract:

    summary The core subgenomic promoter for the initiation of Cucumber Mosaic Virus (CMV) RNA4A was characterized in vitro using a template-dependent RNA synthesis assay and variants of the core promoter RNAs. The minimal sequence required for specific initiation from the cytidylate (T1) used in vivo consists of 31-nucleotides (nt) 3' of T1 and a 13 nt template sequence. This 44 nt RNA was found to provide three elements that contribute to efficient initiation of RNA4A synthesis by the CMV replicase: a stem-loop secondary structure 3' of T1, a template sequence that is rich in adenylates and uridylates, and T1 in an unbase-paired sequence.

Bajrang Singh - One of the best experts on this subject based on the ideXlab platform.

  • Properties of a Cucumber Mosaic Virus strain naturally infecting chrysanthemum in India
    Plant Disease, 1992
    Co-Authors: K. M. Srivastava, Shri Krishna Raj, Bajrang Singh
    Abstract:

    Diffused chlorosis, chlorotic dots near veins, and stunting plants were observed in Chrysanthemum × morifolium. A Virus isolate was purified and characterized as chrysanthemum strain of Cucumber Mosaic Virus (CMV-CI), based on host reaction, in vitro properties, particle morphology (29 nm), molecular weight of coat protein subunit (24.5 kDa), nucleic acid (five RNA species), and serological relationships with other cucumoViruses

E.m.j. Jaspars - One of the best experts on this subject based on the ideXlab platform.

R. N. Campbell - One of the best experts on this subject based on the ideXlab platform.

  • Characterization of Cucumber Mosaic Virus isolates from California
    Plant Disease, 1992
    Co-Authors: J. Daniels, R. N. Campbell
    Abstract:

    Thirty Cucumber Mosaic Virus (CMV) isolates from California were characterized biologically (host reaction and thermosensitivity), serologically (protein A sandwich-enzyme-linked immunosorbent assay [PAS-ELISA]) and physically (viral capsid protein migration in polyacrylamide gel electrophoresis [PAGE], peptide mapping, and migration of viral double-stranded RNAs [dsRNAs] in PAGE). All methods supported the classification of CMV isolates into two main subgroups, CMV-I and CMV-II (.)

K. M. Srivastava - One of the best experts on this subject based on the ideXlab platform.

  • Properties of a Cucumber Mosaic Virus strain naturally infecting chrysanthemum in India
    Plant Disease, 1992
    Co-Authors: K. M. Srivastava, Shri Krishna Raj, Bajrang Singh
    Abstract:

    Diffused chlorosis, chlorotic dots near veins, and stunting plants were observed in Chrysanthemum × morifolium. A Virus isolate was purified and characterized as chrysanthemum strain of Cucumber Mosaic Virus (CMV-CI), based on host reaction, in vitro properties, particle morphology (29 nm), molecular weight of coat protein subunit (24.5 kDa), nucleic acid (five RNA species), and serological relationships with other cucumoViruses