The Experts below are selected from a list of 3804 Experts worldwide ranked by ideXlab platform
Hexun Huang - One of the best experts on this subject based on the ideXlab platform.
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Characterization of Starch in Cucurbita moschata Germplasms throughout Fruit Development.
Journal of Agricultural and Food Chemistry, 2020Co-Authors: Hafiz Muhammad Khalid Abbas, Hexun Huang, Yu-fei Yang, Yuan-heng Xie, Jian-feng Zou, Shu-dan Xue, Dong-guang Song, Li Junxing, Yujuan ZhongAbstract:Pumpkins (Cucurbita moschata; Cucurbitaceae) are the rich source of nutrients and valued for their biologically active substances to be used for the treatment of several diseases. The contents, com...
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The first Illumina-based de novo transcriptome sequencing and analysis of pumpkin (Cucurbita moschata Duch.) and SSR marker development
Molecular Breeding, 2014Co-Authors: Tingquan Wu, Shaobo Luo, Yu’e Lin, Baojuan Sun, Yujuan Zhong, Xiaomei Xu, Xiaoming He, Rui Wang, Hexun HuangAbstract:Pumpkin (Cucurbita moschata Duch.) is an important vegetable crop cultivated worldwide. In this study, the pumpkin transcriptome was sequenced by RNA-seq using the Illumina Hiseq 2000. A total of 52,849,316 clean sequencing reads, 66,621 contigs and 62,480 unigenes were postulated. Based on similarity searches with known proteins, 47,899 genes (76.66 % of the unigenes) were annotated: 47,596, 34,368 and 16,700 mapped in Nr, Swissprot and COG classifica-tions, respectively; 21,164 were annotated with 44 gene ontology functional categories; and 13,728 were anno-tated to 269 pathways by searching the Kyoto Encyclo-pedia of Genes and Genomes pathway database. A total of 7,814 simple sequence repeats (SSRs) were identified in these unigenes and 4,794 pairs of primers were designed for application of SSRs. To date, 35 SSRs have been validated in 12 pumpkin varieties and can separate the pumpkin varieties into Cucurbita maxima and Cucurbita moschata. In addition, the expression of eight photoperiod-related unigenes were studied in different pumpkin plants and it was deduced that they may contribute to late flowering and light insensitive-ness. This research will provide an important platform to facilitate gene discovery for functional genome studies of pumpkin and to conduct SSR discovery for breeders for use in pumpkin breeding.
Tingquan Wu - One of the best experts on this subject based on the ideXlab platform.
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The first Illumina-based de novo transcriptome sequencing and analysis of pumpkin (Cucurbita moschata Duch.) and SSR marker development
Molecular Breeding, 2014Co-Authors: Tingquan Wu, Shaobo Luo, Yu’e Lin, Baojuan Sun, Yujuan Zhong, Xiaomei Xu, Xiaoming He, Rui Wang, Hexun HuangAbstract:Pumpkin (Cucurbita moschata Duch.) is an important vegetable crop cultivated worldwide. In this study, the pumpkin transcriptome was sequenced by RNA-seq using the Illumina Hiseq 2000. A total of 52,849,316 clean sequencing reads, 66,621 contigs and 62,480 unigenes were postulated. Based on similarity searches with known proteins, 47,899 genes (76.66 % of the unigenes) were annotated: 47,596, 34,368 and 16,700 mapped in Nr, Swissprot and COG classifica-tions, respectively; 21,164 were annotated with 44 gene ontology functional categories; and 13,728 were anno-tated to 269 pathways by searching the Kyoto Encyclo-pedia of Genes and Genomes pathway database. A total of 7,814 simple sequence repeats (SSRs) were identified in these unigenes and 4,794 pairs of primers were designed for application of SSRs. To date, 35 SSRs have been validated in 12 pumpkin varieties and can separate the pumpkin varieties into Cucurbita maxima and Cucurbita moschata. In addition, the expression of eight photoperiod-related unigenes were studied in different pumpkin plants and it was deduced that they may contribute to late flowering and light insensitive-ness. This research will provide an important platform to facilitate gene discovery for functional genome studies of pumpkin and to conduct SSR discovery for breeders for use in pumpkin breeding.
Shaobo Luo - One of the best experts on this subject based on the ideXlab platform.
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The first Illumina-based de novo transcriptome sequencing and analysis of pumpkin (Cucurbita moschata Duch.) and SSR marker development
Molecular Breeding, 2014Co-Authors: Tingquan Wu, Shaobo Luo, Yu’e Lin, Baojuan Sun, Yujuan Zhong, Xiaomei Xu, Xiaoming He, Rui Wang, Hexun HuangAbstract:Pumpkin (Cucurbita moschata Duch.) is an important vegetable crop cultivated worldwide. In this study, the pumpkin transcriptome was sequenced by RNA-seq using the Illumina Hiseq 2000. A total of 52,849,316 clean sequencing reads, 66,621 contigs and 62,480 unigenes were postulated. Based on similarity searches with known proteins, 47,899 genes (76.66 % of the unigenes) were annotated: 47,596, 34,368 and 16,700 mapped in Nr, Swissprot and COG classifica-tions, respectively; 21,164 were annotated with 44 gene ontology functional categories; and 13,728 were anno-tated to 269 pathways by searching the Kyoto Encyclo-pedia of Genes and Genomes pathway database. A total of 7,814 simple sequence repeats (SSRs) were identified in these unigenes and 4,794 pairs of primers were designed for application of SSRs. To date, 35 SSRs have been validated in 12 pumpkin varieties and can separate the pumpkin varieties into Cucurbita maxima and Cucurbita moschata. In addition, the expression of eight photoperiod-related unigenes were studied in different pumpkin plants and it was deduced that they may contribute to late flowering and light insensitive-ness. This research will provide an important platform to facilitate gene discovery for functional genome studies of pumpkin and to conduct SSR discovery for breeders for use in pumpkin breeding.
Baojuan Sun - One of the best experts on this subject based on the ideXlab platform.
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The first Illumina-based de novo transcriptome sequencing and analysis of pumpkin (Cucurbita moschata Duch.) and SSR marker development
Molecular Breeding, 2014Co-Authors: Tingquan Wu, Shaobo Luo, Yu’e Lin, Baojuan Sun, Yujuan Zhong, Xiaomei Xu, Xiaoming He, Rui Wang, Hexun HuangAbstract:Pumpkin (Cucurbita moschata Duch.) is an important vegetable crop cultivated worldwide. In this study, the pumpkin transcriptome was sequenced by RNA-seq using the Illumina Hiseq 2000. A total of 52,849,316 clean sequencing reads, 66,621 contigs and 62,480 unigenes were postulated. Based on similarity searches with known proteins, 47,899 genes (76.66 % of the unigenes) were annotated: 47,596, 34,368 and 16,700 mapped in Nr, Swissprot and COG classifica-tions, respectively; 21,164 were annotated with 44 gene ontology functional categories; and 13,728 were anno-tated to 269 pathways by searching the Kyoto Encyclo-pedia of Genes and Genomes pathway database. A total of 7,814 simple sequence repeats (SSRs) were identified in these unigenes and 4,794 pairs of primers were designed for application of SSRs. To date, 35 SSRs have been validated in 12 pumpkin varieties and can separate the pumpkin varieties into Cucurbita maxima and Cucurbita moschata. In addition, the expression of eight photoperiod-related unigenes were studied in different pumpkin plants and it was deduced that they may contribute to late flowering and light insensitive-ness. This research will provide an important platform to facilitate gene discovery for functional genome studies of pumpkin and to conduct SSR discovery for breeders for use in pumpkin breeding.
Yu’e Lin - One of the best experts on this subject based on the ideXlab platform.
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The first Illumina-based de novo transcriptome sequencing and analysis of pumpkin (Cucurbita moschata Duch.) and SSR marker development
Molecular Breeding, 2014Co-Authors: Tingquan Wu, Shaobo Luo, Yu’e Lin, Baojuan Sun, Yujuan Zhong, Xiaomei Xu, Xiaoming He, Rui Wang, Hexun HuangAbstract:Pumpkin (Cucurbita moschata Duch.) is an important vegetable crop cultivated worldwide. In this study, the pumpkin transcriptome was sequenced by RNA-seq using the Illumina Hiseq 2000. A total of 52,849,316 clean sequencing reads, 66,621 contigs and 62,480 unigenes were postulated. Based on similarity searches with known proteins, 47,899 genes (76.66 % of the unigenes) were annotated: 47,596, 34,368 and 16,700 mapped in Nr, Swissprot and COG classifica-tions, respectively; 21,164 were annotated with 44 gene ontology functional categories; and 13,728 were anno-tated to 269 pathways by searching the Kyoto Encyclo-pedia of Genes and Genomes pathway database. A total of 7,814 simple sequence repeats (SSRs) were identified in these unigenes and 4,794 pairs of primers were designed for application of SSRs. To date, 35 SSRs have been validated in 12 pumpkin varieties and can separate the pumpkin varieties into Cucurbita maxima and Cucurbita moschata. In addition, the expression of eight photoperiod-related unigenes were studied in different pumpkin plants and it was deduced that they may contribute to late flowering and light insensitive-ness. This research will provide an important platform to facilitate gene discovery for functional genome studies of pumpkin and to conduct SSR discovery for breeders for use in pumpkin breeding.