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Saadia Mahari - One of the best experts on this subject based on the ideXlab platform.
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evaluation preliminaire de l activite larvicide des extraits aqueux des feuilles du ricin ricinus communis l et du bois de thuya tetraclinis articulata vahl mast sur les larves de quatre moustiques culicides culex pipiens linne aedes caspius pallas c
Biotechnologie Agronomie Société et Environnement, 2006Co-Authors: Brahim Aouinty, Saadia Oufara, Fouad Mellouki, Saadia MahariAbstract:Les extraits aqueux des feuilles du ricin Ricinus communis L. et du bois de thuya Tetraclinis articulata (Vahl) Mast. presentent des effets toxiques sur des larves de moustiques culicides. Dans ce travail, l’activite insecticide de ces extraits a ete etudiee sur les larves des stades 2 et 4 de Culex pipiens (Linne), Aedes caspius (Pallas), Culiseta longiareolata (Aitken) et Anopheles maculipennis (Meigen). Les tests de toxicite ont revele au bout de 24 heures d’exposition des concentrations letales CL50 tres faibles. Dans le cadre de lutte anti-moustique, les extraits de ces plantes peuvent etre utilises comme des biocides naturels.
John J. Howard - One of the best experts on this subject based on the ideXlab platform.
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Cases of Eastern equine encephalitis in humans associated with Aedes canadensis, Coquillettidia perturbans and Culiseta melanura mosquitoes with the virus in New York State from 1971 to 2012 by analysis of aggregated published data.
Epidemiology and Infection, 2020Co-Authors: James A. Sherwood, John J. Howard, S. V. Stehman, Joanne OliverAbstract:From 1971 to 2012, in New York State, years with human Eastern equine encephalitis (EEE) were more strongly associated with the presence of Aedes canadensis, Coquillettidia perturbans and Culiseta melanura mosquitoes infected with the EEE virus (Fisher's exact test, one-sided P = 0.005, 0.03, 0.03) than with Culiseta morsitans, Aedes vexans, Culex pipiens-restuans, Anopheles quadrimaculatus or Anopheles punctipennis (P = 0.05, 0.40, 0.33, 1.00, 1.00). The estimated relative risk of a case in a year in which the virus was detected vs. not detected was 14.67 for Ae. canadensis, 6.38 for Cq. perturbans and 5.50 for Cs. morsitans. In all 5 years with a case, Cs. melanura with the virus was detected. In no year was there a case in the absence of Cs. melanura with the virus. There were 18 years with no case in the presence of Cs. melanura with the virus. Such observations may identify the time of increased risk, and when the methods may be used to prevent or reduce exposure to vector mosquito species in this geographic region.
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Twenty years of surveillance for Eastern equine encephalitis virus in mosquitoes in New York State from 1993 to 2012
Parasites & Vectors, 2018Co-Authors: Joanne Oliver, Laura D Kramer, James A. Sherwood, Gary Lukacik, John Kokas, Scott R. Campbell, John J. HowardAbstract:Background The year 1971 was the first time in New York State (NYS) that Eastern equine encephalitis virus (EEEV) was identified in mosquitoes, in Culiseta melanura and Culiseta morsitans . At that time, state and county health departments began surveillance for EEEV in mosquitoes. Methods From 1993 to 2012, county health departments continued voluntary participation with the state health department in mosquito and arbovirus surveillance. Adult female mosquitoes were trapped, identified, and pooled. Mosquito pools were tested for EEEV by Vero cell culture each of the twenty years. Beginning in 2000, mosquito extracts and cell culture supernatant were tested by reverse transcriptase-polymerase chain reaction (RT-PCR). Results During the years 1993 to 2012, EEEV was identified in: Culiseta melanura , Culiseta morsitans, Coquillettidia perturbans , Aedes canadensis ( Ochlerotatus canadensis ), Aedes vexans , Anopheles punctipennis , Anopheles quadrimaculatus , Psorophora ferox , Culex salinarius , and Culex pipiens-restuans group. EEEV was detected in 427 adult mosquito pools of 107,156 pools tested totaling 3.96 million mosquitoes. Detections of EEEV occurred in three geographical regions of NYS: Sullivan County, Suffolk County, and the contiguous counties of Madison, Oneida, Onondaga and Oswego. Detections of EEEV in mosquitoes occurred every year from 2003 to 2012, inclusive. EEEV was not detected in 1995, and 1998 to 2002, inclusive. Conclusions This was the first time in NYS that EEEV was detected in Cx. salinarius , Ps. ferox and An. punctipennis . The detection of EEEV in mosquitoes every year for 10 years was the longest time span since surveillance began in 1971. The calendar date of the earliest annual appearance of EEEV in mosquitoes did not change during surveillance spanning 42 years.
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molecular identification of blood meal sources in Culiseta melanura and Culiseta morsitans from an endemic focus of eastern equine encephalitis virus in new york
American Journal of Tropical Medicine and Hygiene, 2006Co-Authors: Goudarz Molaei, Philip M Armstrong, Theodore G Andreadis, Joanne Oliver, John J. HowardAbstract:Eastern equine encephalitis (EEE) virus perpetuates in an enzootic cycle involving ornithophilic mosquito vectors, principally Culiseta melanura (Coquillett) and avian amplification hosts. To better understand the role of Cs. melanura and Culiseta morsitans (Theobald) in the epizootiology of EEE virus, we collected blood-fed mosquitoes between 31 May and 15 October 2004 at two sites associated with an EEE virus focus in central New York and identified the source of vertebrate blood by nucleotide sequencing of polymerase chain reaction (PCR) products of the cytochrome b gene. Analysis of 484 Cs. melanura and 122 Cs. morsitans revealed that 94.2% and 86.9%, respectively, acquired blood solely from avian hosts. Blood meals derived exclusively from mammals were detected in 0.8% of Cs. melanura and 1.6% of Cs. morsitans. Individual mosquitoes containing mixed-blood meals from both avian and mammalian hosts were also detected in 5.0% of Cs. melanura and 11.5% of Cs. morsitans. Wood thrush constituted the most common vertebrate host for Cs. melanura (23.6%) and Cs. morsitans (30.9%), followed by American robin, song sparrow, ovenbird, red-eyed vireo, and common yellowthroat. Mammalian-derived blood meals were identified as white-tailed deer, horse, domestic cat, and eastern pipistrelle bat. There were three isolations of EEE virus from Cs. melanura and one from Cs. morsitans. These results suggest that wood thrush and a few other passerine birds may play key roles in supporting EEE virus transmission in the northeast and possibly throughout the geographic range of EEE in North America. The frequency of mammalian feedings also suggests that Cs. melanura and Cs. morsitans may play a role in the transmission of EEE virus to equines, in addition to maintaining enzootic transmission among avian hosts. We report the first isolation of arboviruses from mosquito vectors concomitant with the identifications of their blood meal sources.
Theodore G Andreadis - One of the best experts on this subject based on the ideXlab platform.
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Heterozygosity and allelic diversity in eastern populations of Culiseta melanura.
2018Co-Authors: John Soghigian, Theodore G Andreadis, Goudarz MolaeiAbstract:Heterozygosity and allelic diversity in eastern populations of Culiseta melanura.
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The BUSCO results for five publicly available Dipteran vector genomes, and the Culiseta melanura draft genome polished with Arrow.
2018Co-Authors: John Soghigian, Theodore G Andreadis, Goudarz MolaeiAbstract:The draft genome generated in this study for Culiseta melanura has similar levels of completeness to several other vector genomes, with at least partial evidence of 88.7% single copy orthologs.
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Cx. salinarius, and Culiseta melanura
2013Co-Authors: Theodore G Andreadis, John F Anderson, Charles R. VossbrinckAbstract:Fourteen isolations of West Nile (WN) virus were obtained from four mosquito species (Culex pipiens [5], Cx. restuans [4], Cx. salinarius [2], and Culiseta melanura [3]) in statewide surveillance conducted from June through October 2000. Most isolates were obtained from mosquitoes collected in densely populated residential locales in Fairfield and New Haven counties, where the highest rates of dead crow sightings were reported and where WN virus was detected in 1999. Minimum field infection rates per 1,000 mosquitoes ranged from 0.5 to 1.8 (county based) and from 1.3 to 76.9 (site specific). Cx. restuans appears to be important in initiating WN virus transmission among birds in early summer; Cx. pipiens appears to play a greater role in amplifying virus later in the season. Cs. melanura could be important in the circulation of WN virus among birds in sylvan environments; Cx. salinarius is a suspected vector of WN virus to humans and horses. Epizootic West Nile (WN) virus activity was first detected in Connecticut during September and October 1999 (1). Substantial die-offs among American Crows, Corvus brachyrhynchos, was observed along a 100-km corrido
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molecular identification of blood meal sources in Culiseta melanura and Culiseta morsitans from an endemic focus of eastern equine encephalitis virus in new york
American Journal of Tropical Medicine and Hygiene, 2006Co-Authors: Goudarz Molaei, Philip M Armstrong, Theodore G Andreadis, Joanne Oliver, John J. HowardAbstract:Eastern equine encephalitis (EEE) virus perpetuates in an enzootic cycle involving ornithophilic mosquito vectors, principally Culiseta melanura (Coquillett) and avian amplification hosts. To better understand the role of Cs. melanura and Culiseta morsitans (Theobald) in the epizootiology of EEE virus, we collected blood-fed mosquitoes between 31 May and 15 October 2004 at two sites associated with an EEE virus focus in central New York and identified the source of vertebrate blood by nucleotide sequencing of polymerase chain reaction (PCR) products of the cytochrome b gene. Analysis of 484 Cs. melanura and 122 Cs. morsitans revealed that 94.2% and 86.9%, respectively, acquired blood solely from avian hosts. Blood meals derived exclusively from mammals were detected in 0.8% of Cs. melanura and 1.6% of Cs. morsitans. Individual mosquitoes containing mixed-blood meals from both avian and mammalian hosts were also detected in 5.0% of Cs. melanura and 11.5% of Cs. morsitans. Wood thrush constituted the most common vertebrate host for Cs. melanura (23.6%) and Cs. morsitans (30.9%), followed by American robin, song sparrow, ovenbird, red-eyed vireo, and common yellowthroat. Mammalian-derived blood meals were identified as white-tailed deer, horse, domestic cat, and eastern pipistrelle bat. There were three isolations of EEE virus from Cs. melanura and one from Cs. morsitans. These results suggest that wood thrush and a few other passerine birds may play key roles in supporting EEE virus transmission in the northeast and possibly throughout the geographic range of EEE in North America. The frequency of mammalian feedings also suggests that Cs. melanura and Cs. morsitans may play a role in the transmission of EEE virus to equines, in addition to maintaining enzootic transmission among avian hosts. We report the first isolation of arboviruses from mosquito vectors concomitant with the identifications of their blood meal sources.
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Molecular phylogeny and evolutionary relationships among mosquitoes (Diptera: Culicidae) from the northeastern United States based on small subunit ribosomal DNA (18S rDNA) sequences.
Journal of medical entomology, 2006Co-Authors: John J. Shepard, Theodore G Andreadis, Charles R. VossbrinckAbstract:The phylogenetic relationships of Culicidae native to the northeastern United States were investigated by analyzing small subunit ribosomal DNA (18S rDNA) sequences obtained from 39 species representing nine genera. Molecular phylogenies were consistent with traditional classifications based on morphological characters except for the placements of Psorophora Robineau-Desvoidy and Uranotaenia Lynch Arribalzaga. In our analyses, 1) Anopheles Meigen was strongly supported as the sister taxon to the remaining Culicidae; 2) Toxorhynchites Theobald was represented as a distinct monophyletic sister group to the Culicinae; 3) Psorophora formed a basal clade to Culiseta Felt, Coquillettidia Dyar, and Culex L. but also was shown as a sister taxon to Aedes Meigen and Ochlerotatus Lynch Arribalzaga; 4) Coquillettidia perturbans (Walker) seems to be a sister group to Culiseta; 5) placement of Uranotaenia was inconclusive and seemed to be either a sister group to the Aedes and Ochlerotatus or a basal taxon to all other culicines; and 6) Aedes and Ochlerotatus formed two separate and distinct clades, providing phylogenetic data consistent with the recent elevation of Ochlerotatus to the generic level as proposed by Reinert (2000). The utility of 18S rDNA for evaluating phylogenetic and evolutionary relationships among mosquito taxa was demonstrated at the genus and species levels. To our knowledge, this study represents the first molecular-based phylogenetic study of mosquito species occurring within this geographic region of North America and contains the largest number of species that have been examined among the genera Aedes and Ochlerotatus.
Joanne Oliver - One of the best experts on this subject based on the ideXlab platform.
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Cases of Eastern equine encephalitis in humans associated with Aedes canadensis, Coquillettidia perturbans and Culiseta melanura mosquitoes with the virus in New York State from 1971 to 2012 by analysis of aggregated published data.
Epidemiology and Infection, 2020Co-Authors: James A. Sherwood, John J. Howard, S. V. Stehman, Joanne OliverAbstract:From 1971 to 2012, in New York State, years with human Eastern equine encephalitis (EEE) were more strongly associated with the presence of Aedes canadensis, Coquillettidia perturbans and Culiseta melanura mosquitoes infected with the EEE virus (Fisher's exact test, one-sided P = 0.005, 0.03, 0.03) than with Culiseta morsitans, Aedes vexans, Culex pipiens-restuans, Anopheles quadrimaculatus or Anopheles punctipennis (P = 0.05, 0.40, 0.33, 1.00, 1.00). The estimated relative risk of a case in a year in which the virus was detected vs. not detected was 14.67 for Ae. canadensis, 6.38 for Cq. perturbans and 5.50 for Cs. morsitans. In all 5 years with a case, Cs. melanura with the virus was detected. In no year was there a case in the absence of Cs. melanura with the virus. There were 18 years with no case in the presence of Cs. melanura with the virus. Such observations may identify the time of increased risk, and when the methods may be used to prevent or reduce exposure to vector mosquito species in this geographic region.
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Twenty years of surveillance for Eastern equine encephalitis virus in mosquitoes in New York State from 1993 to 2012
Parasites & Vectors, 2018Co-Authors: Joanne Oliver, Laura D Kramer, James A. Sherwood, Gary Lukacik, John Kokas, Scott R. Campbell, John J. HowardAbstract:Background The year 1971 was the first time in New York State (NYS) that Eastern equine encephalitis virus (EEEV) was identified in mosquitoes, in Culiseta melanura and Culiseta morsitans . At that time, state and county health departments began surveillance for EEEV in mosquitoes. Methods From 1993 to 2012, county health departments continued voluntary participation with the state health department in mosquito and arbovirus surveillance. Adult female mosquitoes were trapped, identified, and pooled. Mosquito pools were tested for EEEV by Vero cell culture each of the twenty years. Beginning in 2000, mosquito extracts and cell culture supernatant were tested by reverse transcriptase-polymerase chain reaction (RT-PCR). Results During the years 1993 to 2012, EEEV was identified in: Culiseta melanura , Culiseta morsitans, Coquillettidia perturbans , Aedes canadensis ( Ochlerotatus canadensis ), Aedes vexans , Anopheles punctipennis , Anopheles quadrimaculatus , Psorophora ferox , Culex salinarius , and Culex pipiens-restuans group. EEEV was detected in 427 adult mosquito pools of 107,156 pools tested totaling 3.96 million mosquitoes. Detections of EEEV occurred in three geographical regions of NYS: Sullivan County, Suffolk County, and the contiguous counties of Madison, Oneida, Onondaga and Oswego. Detections of EEEV in mosquitoes occurred every year from 2003 to 2012, inclusive. EEEV was not detected in 1995, and 1998 to 2002, inclusive. Conclusions This was the first time in NYS that EEEV was detected in Cx. salinarius , Ps. ferox and An. punctipennis . The detection of EEEV in mosquitoes every year for 10 years was the longest time span since surveillance began in 1971. The calendar date of the earliest annual appearance of EEEV in mosquitoes did not change during surveillance spanning 42 years.
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molecular identification of blood meal sources in Culiseta melanura and Culiseta morsitans from an endemic focus of eastern equine encephalitis virus in new york
American Journal of Tropical Medicine and Hygiene, 2006Co-Authors: Goudarz Molaei, Philip M Armstrong, Theodore G Andreadis, Joanne Oliver, John J. HowardAbstract:Eastern equine encephalitis (EEE) virus perpetuates in an enzootic cycle involving ornithophilic mosquito vectors, principally Culiseta melanura (Coquillett) and avian amplification hosts. To better understand the role of Cs. melanura and Culiseta morsitans (Theobald) in the epizootiology of EEE virus, we collected blood-fed mosquitoes between 31 May and 15 October 2004 at two sites associated with an EEE virus focus in central New York and identified the source of vertebrate blood by nucleotide sequencing of polymerase chain reaction (PCR) products of the cytochrome b gene. Analysis of 484 Cs. melanura and 122 Cs. morsitans revealed that 94.2% and 86.9%, respectively, acquired blood solely from avian hosts. Blood meals derived exclusively from mammals were detected in 0.8% of Cs. melanura and 1.6% of Cs. morsitans. Individual mosquitoes containing mixed-blood meals from both avian and mammalian hosts were also detected in 5.0% of Cs. melanura and 11.5% of Cs. morsitans. Wood thrush constituted the most common vertebrate host for Cs. melanura (23.6%) and Cs. morsitans (30.9%), followed by American robin, song sparrow, ovenbird, red-eyed vireo, and common yellowthroat. Mammalian-derived blood meals were identified as white-tailed deer, horse, domestic cat, and eastern pipistrelle bat. There were three isolations of EEE virus from Cs. melanura and one from Cs. morsitans. These results suggest that wood thrush and a few other passerine birds may play key roles in supporting EEE virus transmission in the northeast and possibly throughout the geographic range of EEE in North America. The frequency of mammalian feedings also suggests that Cs. melanura and Cs. morsitans may play a role in the transmission of EEE virus to equines, in addition to maintaining enzootic transmission among avian hosts. We report the first isolation of arboviruses from mosquito vectors concomitant with the identifications of their blood meal sources.
Brahim Aouinty - One of the best experts on this subject based on the ideXlab platform.
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evaluation preliminaire de l activite larvicide des extraits aqueux des feuilles du ricin ricinus communis l et du bois de thuya tetraclinis articulata vahl mast sur les larves de quatre moustiques culicides culex pipiens linne aedes caspius pallas c
Biotechnologie Agronomie Société et Environnement, 2006Co-Authors: Brahim Aouinty, Saadia Oufara, Fouad Mellouki, Saadia MahariAbstract:Les extraits aqueux des feuilles du ricin Ricinus communis L. et du bois de thuya Tetraclinis articulata (Vahl) Mast. presentent des effets toxiques sur des larves de moustiques culicides. Dans ce travail, l’activite insecticide de ces extraits a ete etudiee sur les larves des stades 2 et 4 de Culex pipiens (Linne), Aedes caspius (Pallas), Culiseta longiareolata (Aitken) et Anopheles maculipennis (Meigen). Les tests de toxicite ont revele au bout de 24 heures d’exposition des concentrations letales CL50 tres faibles. Dans le cadre de lutte anti-moustique, les extraits de ces plantes peuvent etre utilises comme des biocides naturels.