The Experts below are selected from a list of 267 Experts worldwide ranked by ideXlab platform
Gabriel E. Wagner - One of the best experts on this subject based on the ideXlab platform.
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Erythritol as a single carbon source improves Cultural Isolation of Burkholderia pseudomallei from rice paddy soils.
PLoS neglected tropical diseases, 2019Co-Authors: Trung T. Trinh, Karoline Assig, Quyen T. L. Tran, André Goehler, Linh N. H. Bui, Claudia Wiede, Bettina Folli, Sabine Lichtenegger, Tinh T. Nguyen, Gabriel E. WagnerAbstract:Background Isolation of the soil bacterium Burkholderia pseudomallei from tropical environments is important to generate a global risk map for man and animals to acquire the infectious disease melioidosis. There is increasing evidence, that the currently recommended soil culture protocol using threonine-basal salt solution with colistin (TBSS-C50) for enrichment of B. pseudomallei and Ashdown agar for subsequent subculture lacks sensitivity. We therefore investigated, if the otherwise rarely encountered erythritol catabolism of B. pseudomallei might be exploited to improve Isolation of this bacterium from soil. Methodology/Principal findings Based on TBSS-C50, we designed a new colistin-containing medium with erythritol as the single carbon source (EM). This medium was validated in various culture protocols by analyzing 80 soil samples from 16 different rice fields in Vietnam. B. pseudomallei enrichment was determined in all culture supernatants by a specific quantitative PCR (qPCR) targeting the type three secretion system 1. 51 out of 80 (63.8%) soil samples gave a positive qPCR signal in at least one of the culture conditions. We observed a significantly higher enrichment shown by lower median cycle threshold values for B. pseudomallei in a two-step culture with TBSS-C50 for 48 h followed by EM for 96h compared to single cultures in TBSS-C50 for either 48h or 144h (p
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erythritol as a single carbon source improves Cultural Isolation of burkholderia pseudomallei from rice paddy soils
PLOS Neglected Tropical Diseases, 2019Co-Authors: Trung T. Trinh, Karoline Assig, Quyen T. L. Tran, André Goehler, Linh N. H. Bui, Claudia Wiede, Bettina Folli, Sabine Lichtenegger, Tinh T. Nguyen, Gabriel E. WagnerAbstract:Background Isolation of the soil bacterium Burkholderia pseudomallei from tropical environments is important to generate a global risk map for man and animals to acquire the infectious disease melioidosis. There is increasing evidence, that the currently recommended soil culture protocol using threonine-basal salt solution with colistin (TBSS-C50) for enrichment of B. pseudomallei and Ashdown agar for subsequent subculture lacks sensitivity. We therefore investigated, if the otherwise rarely encountered erythritol catabolism of B. pseudomallei might be exploited to improve Isolation of this bacterium from soil. Methodology/Principal findings Based on TBSS-C50, we designed a new colistin-containing medium with erythritol as the single carbon source (EM). This medium was validated in various culture protocols by analyzing 80 soil samples from 16 different rice fields in Vietnam. B. pseudomallei enrichment was determined in all culture supernatants by a specific quantitative PCR (qPCR) targeting the type three secretion system 1. 51 out of 80 (63.8%) soil samples gave a positive qPCR signal in at least one of the culture conditions. We observed a significantly higher enrichment shown by lower median cycle threshold values for B. pseudomallei in a two-step culture with TBSS-C50 for 48 h followed by EM for 96h compared to single cultures in TBSS-C50 for either 48h or 144h (p<0.0001, respectively). Accordingly, B. pseudomallei could be isolated on Ashdown agar in 58.8% (30/51) of samples after subcultures from our novel two-step enrichment culture compared to only 9.8% (5/51) after standard enrichment with TBSS-C50 for 48h (p<0.0001) or 25.5% (13/51; p<0.01) after TBSS-C50 for 144h. Conclusions/significance In the present study, we show that specific exploitation of B. pseudomallei metabolic capabilities in enrichment protocols leads to a significantly improved Isolation rate of this pathogen from soil compared to established standard procedures. Our new culture method might help to facilitate the creation of environmental risk maps for melioidosis in the future.
Trung T. Trinh - One of the best experts on this subject based on the ideXlab platform.
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Erythritol as a single carbon source improves Cultural Isolation of Burkholderia pseudomallei from rice paddy soils.
PLoS neglected tropical diseases, 2019Co-Authors: Trung T. Trinh, Karoline Assig, Quyen T. L. Tran, André Goehler, Linh N. H. Bui, Claudia Wiede, Bettina Folli, Sabine Lichtenegger, Tinh T. Nguyen, Gabriel E. WagnerAbstract:Background Isolation of the soil bacterium Burkholderia pseudomallei from tropical environments is important to generate a global risk map for man and animals to acquire the infectious disease melioidosis. There is increasing evidence, that the currently recommended soil culture protocol using threonine-basal salt solution with colistin (TBSS-C50) for enrichment of B. pseudomallei and Ashdown agar for subsequent subculture lacks sensitivity. We therefore investigated, if the otherwise rarely encountered erythritol catabolism of B. pseudomallei might be exploited to improve Isolation of this bacterium from soil. Methodology/Principal findings Based on TBSS-C50, we designed a new colistin-containing medium with erythritol as the single carbon source (EM). This medium was validated in various culture protocols by analyzing 80 soil samples from 16 different rice fields in Vietnam. B. pseudomallei enrichment was determined in all culture supernatants by a specific quantitative PCR (qPCR) targeting the type three secretion system 1. 51 out of 80 (63.8%) soil samples gave a positive qPCR signal in at least one of the culture conditions. We observed a significantly higher enrichment shown by lower median cycle threshold values for B. pseudomallei in a two-step culture with TBSS-C50 for 48 h followed by EM for 96h compared to single cultures in TBSS-C50 for either 48h or 144h (p
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erythritol as a single carbon source improves Cultural Isolation of burkholderia pseudomallei from rice paddy soils
PLOS Neglected Tropical Diseases, 2019Co-Authors: Trung T. Trinh, Karoline Assig, Quyen T. L. Tran, André Goehler, Linh N. H. Bui, Claudia Wiede, Bettina Folli, Sabine Lichtenegger, Tinh T. Nguyen, Gabriel E. WagnerAbstract:Background Isolation of the soil bacterium Burkholderia pseudomallei from tropical environments is important to generate a global risk map for man and animals to acquire the infectious disease melioidosis. There is increasing evidence, that the currently recommended soil culture protocol using threonine-basal salt solution with colistin (TBSS-C50) for enrichment of B. pseudomallei and Ashdown agar for subsequent subculture lacks sensitivity. We therefore investigated, if the otherwise rarely encountered erythritol catabolism of B. pseudomallei might be exploited to improve Isolation of this bacterium from soil. Methodology/Principal findings Based on TBSS-C50, we designed a new colistin-containing medium with erythritol as the single carbon source (EM). This medium was validated in various culture protocols by analyzing 80 soil samples from 16 different rice fields in Vietnam. B. pseudomallei enrichment was determined in all culture supernatants by a specific quantitative PCR (qPCR) targeting the type three secretion system 1. 51 out of 80 (63.8%) soil samples gave a positive qPCR signal in at least one of the culture conditions. We observed a significantly higher enrichment shown by lower median cycle threshold values for B. pseudomallei in a two-step culture with TBSS-C50 for 48 h followed by EM for 96h compared to single cultures in TBSS-C50 for either 48h or 144h (p<0.0001, respectively). Accordingly, B. pseudomallei could be isolated on Ashdown agar in 58.8% (30/51) of samples after subcultures from our novel two-step enrichment culture compared to only 9.8% (5/51) after standard enrichment with TBSS-C50 for 48h (p<0.0001) or 25.5% (13/51; p<0.01) after TBSS-C50 for 144h. Conclusions/significance In the present study, we show that specific exploitation of B. pseudomallei metabolic capabilities in enrichment protocols leads to a significantly improved Isolation rate of this pathogen from soil compared to established standard procedures. Our new culture method might help to facilitate the creation of environmental risk maps for melioidosis in the future.
Magda Callis - One of the best experts on this subject based on the ideXlab platform.
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Medped FH: a paradigm for other common monogenic diseases in South Africa.
Atherosclerosis, 1999Co-Authors: Maritha J. Kotze, Magda CallisAbstract:Familial hypercholesterolemia (FH) is the most common autosomal dominant disease in South Africa. A molecular diagnostic service for FH was established in 1990, based on the finding that three low-density lipoprotein receptor LDL-receptor gene defects are responsible for the disease in up to 90% of affected Afrikaners. Subsequent studies have demonstrated that the common LDL-receptor gene mutations are also present in other ethnic groups, most likely due to multiple entries of disease genes into specific populations and/or founder effects coupled to geographic or Cultural Isolation. Increasing numbers of South African FH patients can therefore be characterized at the DNA level using a comprehensive population-directed DNA screening strategy.
Jane Greene - One of the best experts on this subject based on the ideXlab platform.
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Reminiscence Theatre Making Theatre from Memories Pam Schweitzer Reminiscence Theatre Making Theatre from Memories Jessica Kingsley Publishers 272 £19.99 1 84310 430 X 184310430X
Nursing older people, 2007Co-Authors: Jane GreeneAbstract:This book certainly leaves the reader with a very clear understanding of the aim of reminiscence theatre and the diverse nature of approaches. I particularly enjoyed the multiCultural projects that described the Cultural Isolation of older people when they moved to England from overseas.
Linh N. H. Bui - One of the best experts on this subject based on the ideXlab platform.
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Erythritol as a single carbon source improves Cultural Isolation of Burkholderia pseudomallei from rice paddy soils.
PLoS neglected tropical diseases, 2019Co-Authors: Trung T. Trinh, Karoline Assig, Quyen T. L. Tran, André Goehler, Linh N. H. Bui, Claudia Wiede, Bettina Folli, Sabine Lichtenegger, Tinh T. Nguyen, Gabriel E. WagnerAbstract:Background Isolation of the soil bacterium Burkholderia pseudomallei from tropical environments is important to generate a global risk map for man and animals to acquire the infectious disease melioidosis. There is increasing evidence, that the currently recommended soil culture protocol using threonine-basal salt solution with colistin (TBSS-C50) for enrichment of B. pseudomallei and Ashdown agar for subsequent subculture lacks sensitivity. We therefore investigated, if the otherwise rarely encountered erythritol catabolism of B. pseudomallei might be exploited to improve Isolation of this bacterium from soil. Methodology/Principal findings Based on TBSS-C50, we designed a new colistin-containing medium with erythritol as the single carbon source (EM). This medium was validated in various culture protocols by analyzing 80 soil samples from 16 different rice fields in Vietnam. B. pseudomallei enrichment was determined in all culture supernatants by a specific quantitative PCR (qPCR) targeting the type three secretion system 1. 51 out of 80 (63.8%) soil samples gave a positive qPCR signal in at least one of the culture conditions. We observed a significantly higher enrichment shown by lower median cycle threshold values for B. pseudomallei in a two-step culture with TBSS-C50 for 48 h followed by EM for 96h compared to single cultures in TBSS-C50 for either 48h or 144h (p
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erythritol as a single carbon source improves Cultural Isolation of burkholderia pseudomallei from rice paddy soils
PLOS Neglected Tropical Diseases, 2019Co-Authors: Trung T. Trinh, Karoline Assig, Quyen T. L. Tran, André Goehler, Linh N. H. Bui, Claudia Wiede, Bettina Folli, Sabine Lichtenegger, Tinh T. Nguyen, Gabriel E. WagnerAbstract:Background Isolation of the soil bacterium Burkholderia pseudomallei from tropical environments is important to generate a global risk map for man and animals to acquire the infectious disease melioidosis. There is increasing evidence, that the currently recommended soil culture protocol using threonine-basal salt solution with colistin (TBSS-C50) for enrichment of B. pseudomallei and Ashdown agar for subsequent subculture lacks sensitivity. We therefore investigated, if the otherwise rarely encountered erythritol catabolism of B. pseudomallei might be exploited to improve Isolation of this bacterium from soil. Methodology/Principal findings Based on TBSS-C50, we designed a new colistin-containing medium with erythritol as the single carbon source (EM). This medium was validated in various culture protocols by analyzing 80 soil samples from 16 different rice fields in Vietnam. B. pseudomallei enrichment was determined in all culture supernatants by a specific quantitative PCR (qPCR) targeting the type three secretion system 1. 51 out of 80 (63.8%) soil samples gave a positive qPCR signal in at least one of the culture conditions. We observed a significantly higher enrichment shown by lower median cycle threshold values for B. pseudomallei in a two-step culture with TBSS-C50 for 48 h followed by EM for 96h compared to single cultures in TBSS-C50 for either 48h or 144h (p<0.0001, respectively). Accordingly, B. pseudomallei could be isolated on Ashdown agar in 58.8% (30/51) of samples after subcultures from our novel two-step enrichment culture compared to only 9.8% (5/51) after standard enrichment with TBSS-C50 for 48h (p<0.0001) or 25.5% (13/51; p<0.01) after TBSS-C50 for 144h. Conclusions/significance In the present study, we show that specific exploitation of B. pseudomallei metabolic capabilities in enrichment protocols leads to a significantly improved Isolation rate of this pathogen from soil compared to established standard procedures. Our new culture method might help to facilitate the creation of environmental risk maps for melioidosis in the future.