The Experts below are selected from a list of 327 Experts worldwide ranked by ideXlab platform

Eugene Athan - One of the best experts on this subject based on the ideXlab platform.

Josep Guarro - One of the best experts on this subject based on the ideXlab platform.

  • Novel Curvularia species from clinical specimens.
    Persoonia, 2014
    Co-Authors: Hugo Madrid, Deanna A. Sutton, Josep Guarro, Josepa Gene, K.c. Da Cunha, J. Dijksterhuis, Joan Cano, Pedro W. Crous
    Abstract:

    The fungal genus Curvularia includes numerous plant pathogens and some emerging opportunistic pathogens of humans. In a previous study we used morphology and sequences of the nuclear ribosomal internal transcribed spacer region (ITS) and the glyceraldehyde-3-phosphate dehydrogenase (gpd) gene to identify species within a set of 99 clinical Curvularia isolates from the USA. Seventy-two isolates could be identified while the remaining 27 isolates belonged in three unclassified clades that were tentatively labelled Curvularia sp. I, II and III. In the present study, we further assess the taxonomic placement of these isolates using sequences of ITS, gpd, the large subunit rDNA, and the second largest subunit of RNA polymerase II. DNA sequence comparisons with a set of 87 isolates representing 33 Curvularia spp. and members of the closely-related genera Bipolaris and Exserohilum revealed that Curvularia sp. I, II and III represent novel lineages in Curvularia. These lineages are morphologically different from the currently accepted species. In the phylogenetic tree, Curvularia sp. I and sp. III were each split into two distinct lineages. Morphology and phylogeny supported the proposal of five new species, to be named C. americana, C. chlamydospora, C. hominis, C. muehlenbeckiae and C. pseudolunata. The concatenated 4-locus phylogeny revealed the existence of six clades in Curvularia, which are associated with particular morphological features. They were named after representative species, namely americana, eragrostidis, hominis, lunata, spicifera and trifolii.

  • Experimental treatment of Curvularia infection
    Diagnostic Microbiology and Infectious Disease, 2014
    Co-Authors: Katihuska Paredes, Emilio Mayayo, Annette W. Fothergill, Javier Capilla, Deanna A. Sutton, Josep Guarro
    Abstract:

    Abstract We have evaluated the efficacy of amphotericin B, posaconazole, and voriconazole in immunosuppressed murine models of disseminated infection by Curvularia spicifera and Curvularia hawaiiensis . The 3 antifungals improved survival of mice in comparison to controls; however, only the 2 azoles were able to reduce significantly the fungal load.

  • in vitro antifungal susceptibility and molecular identity of 99 clinical isolates of the opportunistic fungal genus Curvularia
    Diagnostic Microbiology and Infectious Disease, 2013
    Co-Authors: Keith Cassia Da Cunha, Annette W. Fothergill, Deanna A. Sutton, Josepa Gene, Josep Cano, Hugo Madrid, Sybren De Hoog, P W Crous, Josep Guarro
    Abstract:

    The in vitro antifungal susceptibility of a set of 99 clinical isolates of Curvularia was tested against 9 drugs using a reference microdilution method. The isolates had been identified previously to species level by comparing their ITS rDNA and glyceraldehyde-3-phosphate dehydrogenase gene sequences with those of reference strains. We were able to reliably identify 73.2% of the isolates, the most frequent species being Curvularia aeria, Curvularia geniculata/Curvularia senegalensis, Curvularia lunata, Curvularia inaequalis, Curvularia verruculosa, and Curvularia borreriae. Most of these isolates had been recovered from nasal sinus, which is generally considered one of the most frequent sites of infection by these fungi. In addition, at least 3 phylogenetic species that have not yet been formally described were detected. The most active drugs were the echinocandins, amphotericin B, and posaconazole, whereas voriconazole and itraconazole showed poor activity.

  • Virulence of Curvularia in a murine model.
    Mycoses, 2013
    Co-Authors: Katihuska Paredes, Emilio Mayayo, Annette W. Fothergill, Javier Capilla, Deanna A. Sutton, Josep Guarro
    Abstract:

    Summary We have evaluated the virulence of two clinically relevant species of Curvularia; Curvularia spicifera and C. hawaiiensis, using an experimental model of disseminated infection in immunocompromised mice. Several inocula were tested over a range 1 × 103–1 × 106 colony-forming units/animal. Both species had a similar behaviour, producing a high mortality. Tissue burden and histopathology studies demonstrated that lung was the organ most affected.

Jason D. Pimentel - One of the best experts on this subject based on the ideXlab platform.

Deanna A. Sutton - One of the best experts on this subject based on the ideXlab platform.

  • Novel Curvularia species from clinical specimens.
    Persoonia, 2014
    Co-Authors: Hugo Madrid, Deanna A. Sutton, Josep Guarro, Josepa Gene, K.c. Da Cunha, J. Dijksterhuis, Joan Cano, Pedro W. Crous
    Abstract:

    The fungal genus Curvularia includes numerous plant pathogens and some emerging opportunistic pathogens of humans. In a previous study we used morphology and sequences of the nuclear ribosomal internal transcribed spacer region (ITS) and the glyceraldehyde-3-phosphate dehydrogenase (gpd) gene to identify species within a set of 99 clinical Curvularia isolates from the USA. Seventy-two isolates could be identified while the remaining 27 isolates belonged in three unclassified clades that were tentatively labelled Curvularia sp. I, II and III. In the present study, we further assess the taxonomic placement of these isolates using sequences of ITS, gpd, the large subunit rDNA, and the second largest subunit of RNA polymerase II. DNA sequence comparisons with a set of 87 isolates representing 33 Curvularia spp. and members of the closely-related genera Bipolaris and Exserohilum revealed that Curvularia sp. I, II and III represent novel lineages in Curvularia. These lineages are morphologically different from the currently accepted species. In the phylogenetic tree, Curvularia sp. I and sp. III were each split into two distinct lineages. Morphology and phylogeny supported the proposal of five new species, to be named C. americana, C. chlamydospora, C. hominis, C. muehlenbeckiae and C. pseudolunata. The concatenated 4-locus phylogeny revealed the existence of six clades in Curvularia, which are associated with particular morphological features. They were named after representative species, namely americana, eragrostidis, hominis, lunata, spicifera and trifolii.

  • Experimental treatment of Curvularia infection
    Diagnostic Microbiology and Infectious Disease, 2014
    Co-Authors: Katihuska Paredes, Emilio Mayayo, Annette W. Fothergill, Javier Capilla, Deanna A. Sutton, Josep Guarro
    Abstract:

    Abstract We have evaluated the efficacy of amphotericin B, posaconazole, and voriconazole in immunosuppressed murine models of disseminated infection by Curvularia spicifera and Curvularia hawaiiensis . The 3 antifungals improved survival of mice in comparison to controls; however, only the 2 azoles were able to reduce significantly the fungal load.

  • in vitro antifungal susceptibility and molecular identity of 99 clinical isolates of the opportunistic fungal genus Curvularia
    Diagnostic Microbiology and Infectious Disease, 2013
    Co-Authors: Keith Cassia Da Cunha, Annette W. Fothergill, Deanna A. Sutton, Josepa Gene, Josep Cano, Hugo Madrid, Sybren De Hoog, P W Crous, Josep Guarro
    Abstract:

    The in vitro antifungal susceptibility of a set of 99 clinical isolates of Curvularia was tested against 9 drugs using a reference microdilution method. The isolates had been identified previously to species level by comparing their ITS rDNA and glyceraldehyde-3-phosphate dehydrogenase gene sequences with those of reference strains. We were able to reliably identify 73.2% of the isolates, the most frequent species being Curvularia aeria, Curvularia geniculata/Curvularia senegalensis, Curvularia lunata, Curvularia inaequalis, Curvularia verruculosa, and Curvularia borreriae. Most of these isolates had been recovered from nasal sinus, which is generally considered one of the most frequent sites of infection by these fungi. In addition, at least 3 phylogenetic species that have not yet been formally described were detected. The most active drugs were the echinocandins, amphotericin B, and posaconazole, whereas voriconazole and itraconazole showed poor activity.

  • Virulence of Curvularia in a murine model.
    Mycoses, 2013
    Co-Authors: Katihuska Paredes, Emilio Mayayo, Annette W. Fothergill, Javier Capilla, Deanna A. Sutton, Josep Guarro
    Abstract:

    Summary We have evaluated the virulence of two clinically relevant species of Curvularia; Curvularia spicifera and C. hawaiiensis, using an experimental model of disseminated infection in immunocompromised mice. Several inocula were tested over a range 1 × 103–1 × 106 colony-forming units/animal. Both species had a similar behaviour, producing a high mortality. Tissue burden and histopathology studies demonstrated that lung was the organ most affected.

Owen C. Harris - One of the best experts on this subject based on the ideXlab platform.