The Experts below are selected from a list of 18 Experts worldwide ranked by ideXlab platform
Huashan Zhang - One of the best experts on this subject based on the ideXlab platform.
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spectrophotometric determination of total protein in serum using a novel near infrared Cyanine Dye 5 5 dicarboxy 1 1 disulfobutyl 3 3 3 3 tetramethylindotricarboCyanine
Analytical and Bioanalytical Chemistry, 2007Co-Authors: Hong Wang, Weirong Li, Huashan ZhangAbstract:The application of near-infrared (NIR) Dyes (λem > 750 nm) to the analysis of biological samples shows much promise, because the long emission wavelengths of such Dyes allow interferences from biomolecule matrices to be minimized. In this paper, a novel NIR Dye, 5,5′-dicarboxy-1,1′-disulfobutyl-3,3,3′,3′-tetramethylindotricarboCyanine (DCDSTCY) has been developed for the spectrophotometric determination of total protein in serum. Under acidic conditions, the binding of DCDSTCY to proteins caused a new peak at 878 nm, the height of which was proportional to the concentration of protein. The linear range of the method was found to be 0.04–0.5 μg mL−1 for bovine serum albumin (BSA) and human serum albumin (HSA), and detection limits of 5 ng mL−1 were obtained for these substances. The maximum binding number of BSA with DCDSTCY was measured to be 133. The method proposed here has been applied to the quantitation of total protein in serum, and recoveries of 96.6–104% were achieved.
Hong Wang - One of the best experts on this subject based on the ideXlab platform.
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spectrophotometric determination of total protein in serum using a novel near infrared Cyanine Dye 5 5 dicarboxy 1 1 disulfobutyl 3 3 3 3 tetramethylindotricarboCyanine
Analytical and Bioanalytical Chemistry, 2007Co-Authors: Hong Wang, Weirong Li, Huashan ZhangAbstract:The application of near-infrared (NIR) Dyes (λem > 750 nm) to the analysis of biological samples shows much promise, because the long emission wavelengths of such Dyes allow interferences from biomolecule matrices to be minimized. In this paper, a novel NIR Dye, 5,5′-dicarboxy-1,1′-disulfobutyl-3,3,3′,3′-tetramethylindotricarboCyanine (DCDSTCY) has been developed for the spectrophotometric determination of total protein in serum. Under acidic conditions, the binding of DCDSTCY to proteins caused a new peak at 878 nm, the height of which was proportional to the concentration of protein. The linear range of the method was found to be 0.04–0.5 μg mL−1 for bovine serum albumin (BSA) and human serum albumin (HSA), and detection limits of 5 ng mL−1 were obtained for these substances. The maximum binding number of BSA with DCDSTCY was measured to be 133. The method proposed here has been applied to the quantitation of total protein in serum, and recoveries of 96.6–104% were achieved.
Weirong Li - One of the best experts on this subject based on the ideXlab platform.
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spectrophotometric determination of total protein in serum using a novel near infrared Cyanine Dye 5 5 dicarboxy 1 1 disulfobutyl 3 3 3 3 tetramethylindotricarboCyanine
Analytical and Bioanalytical Chemistry, 2007Co-Authors: Hong Wang, Weirong Li, Huashan ZhangAbstract:The application of near-infrared (NIR) Dyes (λem > 750 nm) to the analysis of biological samples shows much promise, because the long emission wavelengths of such Dyes allow interferences from biomolecule matrices to be minimized. In this paper, a novel NIR Dye, 5,5′-dicarboxy-1,1′-disulfobutyl-3,3,3′,3′-tetramethylindotricarboCyanine (DCDSTCY) has been developed for the spectrophotometric determination of total protein in serum. Under acidic conditions, the binding of DCDSTCY to proteins caused a new peak at 878 nm, the height of which was proportional to the concentration of protein. The linear range of the method was found to be 0.04–0.5 μg mL−1 for bovine serum albumin (BSA) and human serum albumin (HSA), and detection limits of 5 ng mL−1 were obtained for these substances. The maximum binding number of BSA with DCDSTCY was measured to be 133. The method proposed here has been applied to the quantitation of total protein in serum, and recoveries of 96.6–104% were achieved.
Hussein Samha - One of the best experts on this subject based on the ideXlab platform.
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Molecular Distribution Behavior of Cyanine Dyes in Aqueous Solution
Volume 7 Issue 4, 2009Co-Authors: David Baumann, Bryan Clark, Jared Garlick, Hussein SamhaAbstract:The molecular distribution of the Cyanine Dye (5-chloro-2-[3-[5-chloro-3-(4-sulfobutyl)-2(3H)-benzothiazolylidine]1-propenyl]-3-(4-sulfobutyl)-benzothiazolium hydroxide triethylamine salt, NK-3796) in aqueous solution was investigated using absorption and emission spectroscopy. Dimers of the Dye are formed in concentrated solutions, while monomers dominate more diluted solutions. J aggregates are formed in solutions containing electrolytes such as sodium chloride, calcium chloride, and di-hydrogen ammonium phosphate. The Dye readily forms J aggregates upon mixing with electrolyte. The J aggregates formation was followed spectroscopically by the appearance of a red-shifted narrow band centered at 653 nm in the absorption spectra. A similar shift is also observed in the emission spectra. The absorbance bandwidth is dependent on the charge of the cation of the electrolyte. This suggests different ground-state conformations for the J aggregates in solution.
David Baumann - One of the best experts on this subject based on the ideXlab platform.
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Molecular Distribution Behavior of Cyanine Dyes in Aqueous Solution
Volume 7 Issue 4, 2009Co-Authors: David Baumann, Bryan Clark, Jared Garlick, Hussein SamhaAbstract:The molecular distribution of the Cyanine Dye (5-chloro-2-[3-[5-chloro-3-(4-sulfobutyl)-2(3H)-benzothiazolylidine]1-propenyl]-3-(4-sulfobutyl)-benzothiazolium hydroxide triethylamine salt, NK-3796) in aqueous solution was investigated using absorption and emission spectroscopy. Dimers of the Dye are formed in concentrated solutions, while monomers dominate more diluted solutions. J aggregates are formed in solutions containing electrolytes such as sodium chloride, calcium chloride, and di-hydrogen ammonium phosphate. The Dye readily forms J aggregates upon mixing with electrolyte. The J aggregates formation was followed spectroscopically by the appearance of a red-shifted narrow band centered at 653 nm in the absorption spectra. A similar shift is also observed in the emission spectra. The absorbance bandwidth is dependent on the charge of the cation of the electrolyte. This suggests different ground-state conformations for the J aggregates in solution.
