The Experts below are selected from a list of 285 Experts worldwide ranked by ideXlab platform
Himadri B Pakrasi - One of the best experts on this subject based on the ideXlab platform.
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a reversibly induced crispri system targeting photosystem ii in the Cyanobacterium synechocystis sp pcc 6803
ACS Synthetic Biology, 2020Co-Authors: Virginia M Johnson, Himadri B PakrasiAbstract:The Cyanobacterium Synechocystis sp. PCC 6803 is used as a model organism to study photosynthesis, as it can utilize glucose as the sole carbon source to support its growth under heterotrophic cond...
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high rates of photobiological h2 production by a Cyanobacterium under aerobic conditions
Nature Communications, 2010Co-Authors: Anindita Bandyopadhyay, Jana Stockel, Louis A Sherman, Himadri B PakrasiAbstract:Hydrogen production using photosynthetic bacteria is an appealing energy source, but typically the bacteria require anaerobic conditions. Here, the authors report a wild-type Cyanobacterium strain that shows very high rates of hydrogen production under aerobic environmental conditions.
Masami Yokota Hirai - One of the best experts on this subject based on the ideXlab platform.
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genetic manipulation of a metabolic enzyme and a transcriptional regulator increasing succinate excretion from unicellular Cyanobacterium
Frontiers in Microbiology, 2015Co-Authors: Takashi Osanai, Tomokazu Shirai, Hiroko Iijima, Yuka Nakaya, Mami Okamoto, Akihiko Kondo, Masami Yokota HiraiAbstract:Succinate is a building block compound that the U.S. Department of Energy has declared as important in biorefineries, and it is widely used as a commodity chemical. Here, we identified the two genes increasing succinate production of the unicellular Cyanobacterium Synechocystis sp. PCC 6803. Succinate was excreted under dark, anaerobic conditions, and its production level increased by knocking out ackA, which encodes an acetate kinase, and by overexpressing sigE, which encodes an RNA polymerase sigma factor. Glycogen catabolism and organic acid biosynthesis were enhanced in the mutant lacking ackA and overexpressing sigE, leading to an increase in succinate production reaching 5 times of the wild-type levels. Our genetic and metabolomic analyses thus demonstrated the effect of genetic manipulation of a metabolic enzyme and a transcriptional regulator on succinate excretion from this Cyanobacterium with the data based on metabolomic technique.
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a response regulator rre37 and an rna polymerase sigma factor sige represent two parallel pathways to activate sugar catabolism in a Cyanobacterium synechocystis sp pcc 6803
Plant and Cell Physiology, 2011Co-Authors: Miyuki Azuma, Takashi Osanai, Masami Yokota Hirai, Kan TanakaAbstract:: Sugar catabolic genes are induced during nitrogen starvation in a Cyanobacterium Synechocystis sp. PCC 6803, but the underlying regulatory mechanism still remains to be completely characterized. In this study, we showed by molecular genetics and transcriptome analyses that a response regulator Rre37 (encoded by sll1330), whose expression is enhanced by nitrogen depletion under the control of NtcA, activates transcript accumulation of sugar catabolic genes, such as gap1, pfkA (sll1196), glgP (slr1367) and glgX (slr1857), mainly during nitrogen starvation. Previously, we reported that a group-2 sigma factor SigE also positively regulates sugar catabolic genes in this strain. Phenotypic analyses using a single or double mutant lacking rre37 and/or sigE indicated that both SigE and Rre37 positively regulate sugar catabolic genes independently. These findings substantiated a regulatory network of sugar catabolic genes in this Cyanobacterium.
Kan Tanaka - One of the best experts on this subject based on the ideXlab platform.
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a response regulator rre37 and an rna polymerase sigma factor sige represent two parallel pathways to activate sugar catabolism in a Cyanobacterium synechocystis sp pcc 6803
Plant and Cell Physiology, 2011Co-Authors: Miyuki Azuma, Takashi Osanai, Masami Yokota Hirai, Kan TanakaAbstract:: Sugar catabolic genes are induced during nitrogen starvation in a Cyanobacterium Synechocystis sp. PCC 6803, but the underlying regulatory mechanism still remains to be completely characterized. In this study, we showed by molecular genetics and transcriptome analyses that a response regulator Rre37 (encoded by sll1330), whose expression is enhanced by nitrogen depletion under the control of NtcA, activates transcript accumulation of sugar catabolic genes, such as gap1, pfkA (sll1196), glgP (slr1367) and glgX (slr1857), mainly during nitrogen starvation. Previously, we reported that a group-2 sigma factor SigE also positively regulates sugar catabolic genes in this strain. Phenotypic analyses using a single or double mutant lacking rre37 and/or sigE indicated that both SigE and Rre37 positively regulate sugar catabolic genes independently. These findings substantiated a regulatory network of sugar catabolic genes in this Cyanobacterium.
Takashi Osanai - One of the best experts on this subject based on the ideXlab platform.
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genetic manipulation of a metabolic enzyme and a transcriptional regulator increasing succinate excretion from unicellular Cyanobacterium
Frontiers in Microbiology, 2015Co-Authors: Takashi Osanai, Tomokazu Shirai, Hiroko Iijima, Yuka Nakaya, Mami Okamoto, Akihiko Kondo, Masami Yokota HiraiAbstract:Succinate is a building block compound that the U.S. Department of Energy has declared as important in biorefineries, and it is widely used as a commodity chemical. Here, we identified the two genes increasing succinate production of the unicellular Cyanobacterium Synechocystis sp. PCC 6803. Succinate was excreted under dark, anaerobic conditions, and its production level increased by knocking out ackA, which encodes an acetate kinase, and by overexpressing sigE, which encodes an RNA polymerase sigma factor. Glycogen catabolism and organic acid biosynthesis were enhanced in the mutant lacking ackA and overexpressing sigE, leading to an increase in succinate production reaching 5 times of the wild-type levels. Our genetic and metabolomic analyses thus demonstrated the effect of genetic manipulation of a metabolic enzyme and a transcriptional regulator on succinate excretion from this Cyanobacterium with the data based on metabolomic technique.
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a response regulator rre37 and an rna polymerase sigma factor sige represent two parallel pathways to activate sugar catabolism in a Cyanobacterium synechocystis sp pcc 6803
Plant and Cell Physiology, 2011Co-Authors: Miyuki Azuma, Takashi Osanai, Masami Yokota Hirai, Kan TanakaAbstract:: Sugar catabolic genes are induced during nitrogen starvation in a Cyanobacterium Synechocystis sp. PCC 6803, but the underlying regulatory mechanism still remains to be completely characterized. In this study, we showed by molecular genetics and transcriptome analyses that a response regulator Rre37 (encoded by sll1330), whose expression is enhanced by nitrogen depletion under the control of NtcA, activates transcript accumulation of sugar catabolic genes, such as gap1, pfkA (sll1196), glgP (slr1367) and glgX (slr1857), mainly during nitrogen starvation. Previously, we reported that a group-2 sigma factor SigE also positively regulates sugar catabolic genes in this strain. Phenotypic analyses using a single or double mutant lacking rre37 and/or sigE indicated that both SigE and Rre37 positively regulate sugar catabolic genes independently. These findings substantiated a regulatory network of sugar catabolic genes in this Cyanobacterium.
Virginia M Johnson - One of the best experts on this subject based on the ideXlab platform.
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a reversibly induced crispri system targeting photosystem ii in the Cyanobacterium synechocystis sp pcc 6803
ACS Synthetic Biology, 2020Co-Authors: Virginia M Johnson, Himadri B PakrasiAbstract:The Cyanobacterium Synechocystis sp. PCC 6803 is used as a model organism to study photosynthesis, as it can utilize glucose as the sole carbon source to support its growth under heterotrophic cond...
