The Experts below are selected from a list of 276 Experts worldwide ranked by ideXlab platform
Kerri Pedersen - One of the best experts on this subject based on the ideXlab platform.
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influenza a virus h5 specific antibodies in mute swans Cygnus Olor in the usa
Journal of Wildlife Diseases, 2015Co-Authors: Whitney M. Kistler, David R. Marks, Kerri Pedersen, David E. Stallknecht, Camille Lebarbenchon, Randy Mickley, Thomas J. Deliberto, Michael J. YabsleyAbstract:Abstract The use of serologic assays for influenza A virus (IAV) surveillance in wild birds has increased because of the availability of commercial enzyme-linked immunosorbent assays (ELISAs). Recently, an H5-specific blocking ELISA (bELISA) was shown to reliably detect H5-specific antibodies to low- and high-pathogenic H5 viruses in experimentally infected waterfowl. Mute Swans (Cygnus Olor) were frequently associated with highly pathogenic H5N1 outbreaks in Europe and may have a similar role if highly pathogenic H5N1 is introduced into North America. We measured the prevalence of antibodies to the nucleoprotein and H5 protein in Mute Swans using three serologic assays. We collected 340 serum samples from Mute Swans in Michigan, New Jersey, New York, and Rhode Island, US. We detected antibodies to the IAV nucleoprotein in 66.2% (225/340) of the samples. We detected H5-specific antibodies in 62.9% (214/340) and 18.8% (64/340) using a modified H5 bELISA protocol and hemagglutination inhibition (HI) assay, ...
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Influenza A Virus H5–specific Antibodies in Mute Swans (Cygnus Olor) in the USA
Journal of wildlife diseases, 2015Co-Authors: Whitney M. Kistler, David R. Marks, Kerri Pedersen, David E. Stallknecht, Camille Lebarbenchon, Randy Mickley, Thomas J. Deliberto, Michael J. YabsleyAbstract:Abstract The use of serologic assays for influenza A virus (IAV) surveillance in wild birds has increased because of the availability of commercial enzyme-linked immunosorbent assays (ELISAs). Recently, an H5-specific blocking ELISA (bELISA) was shown to reliably detect H5-specific antibodies to low- and high-pathogenic H5 viruses in experimentally infected waterfowl. Mute Swans (Cygnus Olor) were frequently associated with highly pathogenic H5N1 outbreaks in Europe and may have a similar role if highly pathogenic H5N1 is introduced into North America. We measured the prevalence of antibodies to the nucleoprotein and H5 protein in Mute Swans using three serologic assays. We collected 340 serum samples from Mute Swans in Michigan, New Jersey, New York, and Rhode Island, US. We detected antibodies to the IAV nucleoprotein in 66.2% (225/340) of the samples. We detected H5-specific antibodies in 62.9% (214/340) and 18.8% (64/340) using a modified H5 bELISA protocol and hemagglutination inhibition (HI) assay, ...
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Influenza A Virus H5–specific Antibodies in Mute Swans ( Cygnus Olor ) in the USA
Journal of wildlife diseases, 2015Co-Authors: Whitney M. Kistler, David R. Marks, Kerri Pedersen, David E. Stallknecht, Camille Lebarbenchon, Randy Mickley, Thomas J. Deliberto, Michael J. YabsleyAbstract:The use of serologic assays for influenza A virus (IAV) surveillance in wild birds has increased because of the availability of commercial enzyme-linked immunosorbent assays (ELISAs). Recently, an H5-specific blocking ELISA (bELISA) was shown to reliably detect H5-specific antibodies to low- and high-pathogenic H5 viruses in experimentally infected waterfowl. Mute Swans (Cygnus Olor) were frequently associated with highly pathogenic H5N1 outbreaks in Europe and may have a similar role if highly pathogenic H5N1 is introduced into North America. We measured the prevalence of antibodies to the nucleoprotein and H5 protein in Mute Swans using three serologic assays. We collected 340 serum samples from Mute Swans in Michigan, New Jersey, New York, and Rhode Island, US. We detected antibodies to the IAV nucleoprotein in 66.2% (225/340) of the samples. We detected H5-specific antibodies in 62.9% (214/340) and 18.8% (64/340) using a modified H5 bELISA protocol and hemagglutination inhibition (HI) assay, respectively. The modified H5 bELISA protocol detected significantly more positive samples than did the manufacturer's protocol. We also tested 46 samples using virus neutralization. Neutralization results had high agreement with the modified H5 bELISA protocol and detected a higher prevalence than did the HI assay. These results indicate that North American Mute Swans have high nucleoprotein and H5 antibody prevalences.
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Short report: Antibody prevalence of select arboviruses in mute swans (Cygnus Olor) in the Great Lakes region and Atlantic coast of the United States.
The American journal of tropical medicine and hygiene, 2014Co-Authors: Kerri Pedersen, David R. Marks, Randall M. Mickley, Dustin M. Arsnoe, Sarah N. Bevins, Eryu Wang, Scott C. Weaver, Thomas J. DelibertoAbstract:Mute swans (Cygnus Olor) are an invasive species in the United States. The dramatic increase in their populations in localized areas has led to various problems, among them competition with native species and attacks on humans by aggressive swans. However, very little is known about the ability of these swans to transmit pathogens to humans, domestic birds, or wildlife or participate in enzootic maintenance. To learn more about select pathogens that mute swans may harbor, a survey was conducted from April of 2011 to August of 2012 in the Great Lakes region and localized areas of the Atlantic coast, which revealed serologic evidence of arbovirus exposure in mute swans. Of 497 mute swans tested, antibodies were detected for eastern equine encephalitis (4.8%), St. Louis encephalitis (1.4%), West Nile (1.2%), and Turlock (0.6%) viruses. Samples were also tested for evidence of antibodies to La Crosse virus, but none were positive.
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Avian Paramyxovirus Serotype 1 (Newcastle Disease Virus), Avian Influenza Virus, and Salmonella spp. in Mute Swans (Cygnus Olor) in the Great Lakes Region and Atlantic Coast of the United States
Avian diseases, 2014Co-Authors: Kerri Pedersen, David R. Marks, Adam R. Randall, Dustin M. Arsnoe, Sarah N. Bevins, Claudio L. Afonso, Patti J. Miller, Thomas J. DelibertoAbstract:SUMMARY. Since their introduction to the United States in the late 19th century, mute swans (Cygnus Olor) have become a nuisance species by causing damage to aquatic habitats, acting aggressively toward humans, competing with native waterfowl, and potentially transmitting or serving as a reservoir of infectious diseases to humans and poultry. In an effort to investigate their potential role as a disease reservoir and to establish avian health baselines for pathogens that threaten agricultural species or human health, we collected samples from 858 mute swans and tested them for avian paramyxovirus serotype 1 (APMV-1), avian influenza virus (AIV), and Salmonella spp. when possible. Our results indicate that exposure to APMV-1 and AIV is common (60%, n 5 771, and 45%, n 5 344, antibody prevalence, respectively) in mute swans, but detection of active viral shedding is less common (8.7%, n 5 414, and 0.8%, n 5 390, respectively). Salmonella was isolated from three mute swans (0.6%, n 5 459), and although the serovars identified have been implicated in previous human outbreaks, it does not appear that Salmonella is commonly carried by mute swans.
David R. Marks - One of the best experts on this subject based on the ideXlab platform.
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influenza a virus h5 specific antibodies in mute swans Cygnus Olor in the usa
Journal of Wildlife Diseases, 2015Co-Authors: Whitney M. Kistler, David R. Marks, Kerri Pedersen, David E. Stallknecht, Camille Lebarbenchon, Randy Mickley, Thomas J. Deliberto, Michael J. YabsleyAbstract:Abstract The use of serologic assays for influenza A virus (IAV) surveillance in wild birds has increased because of the availability of commercial enzyme-linked immunosorbent assays (ELISAs). Recently, an H5-specific blocking ELISA (bELISA) was shown to reliably detect H5-specific antibodies to low- and high-pathogenic H5 viruses in experimentally infected waterfowl. Mute Swans (Cygnus Olor) were frequently associated with highly pathogenic H5N1 outbreaks in Europe and may have a similar role if highly pathogenic H5N1 is introduced into North America. We measured the prevalence of antibodies to the nucleoprotein and H5 protein in Mute Swans using three serologic assays. We collected 340 serum samples from Mute Swans in Michigan, New Jersey, New York, and Rhode Island, US. We detected antibodies to the IAV nucleoprotein in 66.2% (225/340) of the samples. We detected H5-specific antibodies in 62.9% (214/340) and 18.8% (64/340) using a modified H5 bELISA protocol and hemagglutination inhibition (HI) assay, ...
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Influenza A Virus H5–specific Antibodies in Mute Swans (Cygnus Olor) in the USA
Journal of wildlife diseases, 2015Co-Authors: Whitney M. Kistler, David R. Marks, Kerri Pedersen, David E. Stallknecht, Camille Lebarbenchon, Randy Mickley, Thomas J. Deliberto, Michael J. YabsleyAbstract:Abstract The use of serologic assays for influenza A virus (IAV) surveillance in wild birds has increased because of the availability of commercial enzyme-linked immunosorbent assays (ELISAs). Recently, an H5-specific blocking ELISA (bELISA) was shown to reliably detect H5-specific antibodies to low- and high-pathogenic H5 viruses in experimentally infected waterfowl. Mute Swans (Cygnus Olor) were frequently associated with highly pathogenic H5N1 outbreaks in Europe and may have a similar role if highly pathogenic H5N1 is introduced into North America. We measured the prevalence of antibodies to the nucleoprotein and H5 protein in Mute Swans using three serologic assays. We collected 340 serum samples from Mute Swans in Michigan, New Jersey, New York, and Rhode Island, US. We detected antibodies to the IAV nucleoprotein in 66.2% (225/340) of the samples. We detected H5-specific antibodies in 62.9% (214/340) and 18.8% (64/340) using a modified H5 bELISA protocol and hemagglutination inhibition (HI) assay, ...
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Influenza A Virus H5–specific Antibodies in Mute Swans ( Cygnus Olor ) in the USA
Journal of wildlife diseases, 2015Co-Authors: Whitney M. Kistler, David R. Marks, Kerri Pedersen, David E. Stallknecht, Camille Lebarbenchon, Randy Mickley, Thomas J. Deliberto, Michael J. YabsleyAbstract:The use of serologic assays for influenza A virus (IAV) surveillance in wild birds has increased because of the availability of commercial enzyme-linked immunosorbent assays (ELISAs). Recently, an H5-specific blocking ELISA (bELISA) was shown to reliably detect H5-specific antibodies to low- and high-pathogenic H5 viruses in experimentally infected waterfowl. Mute Swans (Cygnus Olor) were frequently associated with highly pathogenic H5N1 outbreaks in Europe and may have a similar role if highly pathogenic H5N1 is introduced into North America. We measured the prevalence of antibodies to the nucleoprotein and H5 protein in Mute Swans using three serologic assays. We collected 340 serum samples from Mute Swans in Michigan, New Jersey, New York, and Rhode Island, US. We detected antibodies to the IAV nucleoprotein in 66.2% (225/340) of the samples. We detected H5-specific antibodies in 62.9% (214/340) and 18.8% (64/340) using a modified H5 bELISA protocol and hemagglutination inhibition (HI) assay, respectively. The modified H5 bELISA protocol detected significantly more positive samples than did the manufacturer's protocol. We also tested 46 samples using virus neutralization. Neutralization results had high agreement with the modified H5 bELISA protocol and detected a higher prevalence than did the HI assay. These results indicate that North American Mute Swans have high nucleoprotein and H5 antibody prevalences.
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Short report: Antibody prevalence of select arboviruses in mute swans (Cygnus Olor) in the Great Lakes region and Atlantic coast of the United States.
The American journal of tropical medicine and hygiene, 2014Co-Authors: Kerri Pedersen, David R. Marks, Randall M. Mickley, Dustin M. Arsnoe, Sarah N. Bevins, Eryu Wang, Scott C. Weaver, Thomas J. DelibertoAbstract:Mute swans (Cygnus Olor) are an invasive species in the United States. The dramatic increase in their populations in localized areas has led to various problems, among them competition with native species and attacks on humans by aggressive swans. However, very little is known about the ability of these swans to transmit pathogens to humans, domestic birds, or wildlife or participate in enzootic maintenance. To learn more about select pathogens that mute swans may harbor, a survey was conducted from April of 2011 to August of 2012 in the Great Lakes region and localized areas of the Atlantic coast, which revealed serologic evidence of arbovirus exposure in mute swans. Of 497 mute swans tested, antibodies were detected for eastern equine encephalitis (4.8%), St. Louis encephalitis (1.4%), West Nile (1.2%), and Turlock (0.6%) viruses. Samples were also tested for evidence of antibodies to La Crosse virus, but none were positive.
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Avian Paramyxovirus Serotype 1 (Newcastle Disease Virus), Avian Influenza Virus, and Salmonella spp. in Mute Swans (Cygnus Olor) in the Great Lakes Region and Atlantic Coast of the United States
Avian diseases, 2014Co-Authors: Kerri Pedersen, David R. Marks, Adam R. Randall, Dustin M. Arsnoe, Sarah N. Bevins, Claudio L. Afonso, Patti J. Miller, Thomas J. DelibertoAbstract:SUMMARY. Since their introduction to the United States in the late 19th century, mute swans (Cygnus Olor) have become a nuisance species by causing damage to aquatic habitats, acting aggressively toward humans, competing with native waterfowl, and potentially transmitting or serving as a reservoir of infectious diseases to humans and poultry. In an effort to investigate their potential role as a disease reservoir and to establish avian health baselines for pathogens that threaten agricultural species or human health, we collected samples from 858 mute swans and tested them for avian paramyxovirus serotype 1 (APMV-1), avian influenza virus (AIV), and Salmonella spp. when possible. Our results indicate that exposure to APMV-1 and AIV is common (60%, n 5 771, and 45%, n 5 344, antibody prevalence, respectively) in mute swans, but detection of active viral shedding is less common (8.7%, n 5 414, and 0.8%, n 5 390, respectively). Salmonella was isolated from three mute swans (0.6%, n 5 459), and although the serovars identified have been implicated in previous human outbreaks, it does not appear that Salmonella is commonly carried by mute swans.
Thomas J. Deliberto - One of the best experts on this subject based on the ideXlab platform.
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influenza a virus h5 specific antibodies in mute swans Cygnus Olor in the usa
Journal of Wildlife Diseases, 2015Co-Authors: Whitney M. Kistler, David R. Marks, Kerri Pedersen, David E. Stallknecht, Camille Lebarbenchon, Randy Mickley, Thomas J. Deliberto, Michael J. YabsleyAbstract:Abstract The use of serologic assays for influenza A virus (IAV) surveillance in wild birds has increased because of the availability of commercial enzyme-linked immunosorbent assays (ELISAs). Recently, an H5-specific blocking ELISA (bELISA) was shown to reliably detect H5-specific antibodies to low- and high-pathogenic H5 viruses in experimentally infected waterfowl. Mute Swans (Cygnus Olor) were frequently associated with highly pathogenic H5N1 outbreaks in Europe and may have a similar role if highly pathogenic H5N1 is introduced into North America. We measured the prevalence of antibodies to the nucleoprotein and H5 protein in Mute Swans using three serologic assays. We collected 340 serum samples from Mute Swans in Michigan, New Jersey, New York, and Rhode Island, US. We detected antibodies to the IAV nucleoprotein in 66.2% (225/340) of the samples. We detected H5-specific antibodies in 62.9% (214/340) and 18.8% (64/340) using a modified H5 bELISA protocol and hemagglutination inhibition (HI) assay, ...
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Influenza A Virus H5–specific Antibodies in Mute Swans (Cygnus Olor) in the USA
Journal of wildlife diseases, 2015Co-Authors: Whitney M. Kistler, David R. Marks, Kerri Pedersen, David E. Stallknecht, Camille Lebarbenchon, Randy Mickley, Thomas J. Deliberto, Michael J. YabsleyAbstract:Abstract The use of serologic assays for influenza A virus (IAV) surveillance in wild birds has increased because of the availability of commercial enzyme-linked immunosorbent assays (ELISAs). Recently, an H5-specific blocking ELISA (bELISA) was shown to reliably detect H5-specific antibodies to low- and high-pathogenic H5 viruses in experimentally infected waterfowl. Mute Swans (Cygnus Olor) were frequently associated with highly pathogenic H5N1 outbreaks in Europe and may have a similar role if highly pathogenic H5N1 is introduced into North America. We measured the prevalence of antibodies to the nucleoprotein and H5 protein in Mute Swans using three serologic assays. We collected 340 serum samples from Mute Swans in Michigan, New Jersey, New York, and Rhode Island, US. We detected antibodies to the IAV nucleoprotein in 66.2% (225/340) of the samples. We detected H5-specific antibodies in 62.9% (214/340) and 18.8% (64/340) using a modified H5 bELISA protocol and hemagglutination inhibition (HI) assay, ...
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Influenza A Virus H5–specific Antibodies in Mute Swans ( Cygnus Olor ) in the USA
Journal of wildlife diseases, 2015Co-Authors: Whitney M. Kistler, David R. Marks, Kerri Pedersen, David E. Stallknecht, Camille Lebarbenchon, Randy Mickley, Thomas J. Deliberto, Michael J. YabsleyAbstract:The use of serologic assays for influenza A virus (IAV) surveillance in wild birds has increased because of the availability of commercial enzyme-linked immunosorbent assays (ELISAs). Recently, an H5-specific blocking ELISA (bELISA) was shown to reliably detect H5-specific antibodies to low- and high-pathogenic H5 viruses in experimentally infected waterfowl. Mute Swans (Cygnus Olor) were frequently associated with highly pathogenic H5N1 outbreaks in Europe and may have a similar role if highly pathogenic H5N1 is introduced into North America. We measured the prevalence of antibodies to the nucleoprotein and H5 protein in Mute Swans using three serologic assays. We collected 340 serum samples from Mute Swans in Michigan, New Jersey, New York, and Rhode Island, US. We detected antibodies to the IAV nucleoprotein in 66.2% (225/340) of the samples. We detected H5-specific antibodies in 62.9% (214/340) and 18.8% (64/340) using a modified H5 bELISA protocol and hemagglutination inhibition (HI) assay, respectively. The modified H5 bELISA protocol detected significantly more positive samples than did the manufacturer's protocol. We also tested 46 samples using virus neutralization. Neutralization results had high agreement with the modified H5 bELISA protocol and detected a higher prevalence than did the HI assay. These results indicate that North American Mute Swans have high nucleoprotein and H5 antibody prevalences.
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Short report: Antibody prevalence of select arboviruses in mute swans (Cygnus Olor) in the Great Lakes region and Atlantic coast of the United States.
The American journal of tropical medicine and hygiene, 2014Co-Authors: Kerri Pedersen, David R. Marks, Randall M. Mickley, Dustin M. Arsnoe, Sarah N. Bevins, Eryu Wang, Scott C. Weaver, Thomas J. DelibertoAbstract:Mute swans (Cygnus Olor) are an invasive species in the United States. The dramatic increase in their populations in localized areas has led to various problems, among them competition with native species and attacks on humans by aggressive swans. However, very little is known about the ability of these swans to transmit pathogens to humans, domestic birds, or wildlife or participate in enzootic maintenance. To learn more about select pathogens that mute swans may harbor, a survey was conducted from April of 2011 to August of 2012 in the Great Lakes region and localized areas of the Atlantic coast, which revealed serologic evidence of arbovirus exposure in mute swans. Of 497 mute swans tested, antibodies were detected for eastern equine encephalitis (4.8%), St. Louis encephalitis (1.4%), West Nile (1.2%), and Turlock (0.6%) viruses. Samples were also tested for evidence of antibodies to La Crosse virus, but none were positive.
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Avian Paramyxovirus Serotype 1 (Newcastle Disease Virus), Avian Influenza Virus, and Salmonella spp. in Mute Swans (Cygnus Olor) in the Great Lakes Region and Atlantic Coast of the United States
Avian diseases, 2014Co-Authors: Kerri Pedersen, David R. Marks, Adam R. Randall, Dustin M. Arsnoe, Sarah N. Bevins, Claudio L. Afonso, Patti J. Miller, Thomas J. DelibertoAbstract:SUMMARY. Since their introduction to the United States in the late 19th century, mute swans (Cygnus Olor) have become a nuisance species by causing damage to aquatic habitats, acting aggressively toward humans, competing with native waterfowl, and potentially transmitting or serving as a reservoir of infectious diseases to humans and poultry. In an effort to investigate their potential role as a disease reservoir and to establish avian health baselines for pathogens that threaten agricultural species or human health, we collected samples from 858 mute swans and tested them for avian paramyxovirus serotype 1 (APMV-1), avian influenza virus (AIV), and Salmonella spp. when possible. Our results indicate that exposure to APMV-1 and AIV is common (60%, n 5 771, and 45%, n 5 344, antibody prevalence, respectively) in mute swans, but detection of active viral shedding is less common (8.7%, n 5 414, and 0.8%, n 5 390, respectively). Salmonella was isolated from three mute swans (0.6%, n 5 459), and although the serovars identified have been implicated in previous human outbreaks, it does not appear that Salmonella is commonly carried by mute swans.
Adam R. Randall - One of the best experts on this subject based on the ideXlab platform.
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Avian Paramyxovirus Serotype 1 (Newcastle Disease Virus), Avian Influenza Virus, and Salmonella spp. in Mute Swans (Cygnus Olor) in the Great Lakes Region and Atlantic Coast of the United States
Avian diseases, 2014Co-Authors: Kerri Pedersen, David R. Marks, Adam R. Randall, Dustin M. Arsnoe, Sarah N. Bevins, Claudio L. Afonso, Patti J. Miller, Thomas J. DelibertoAbstract:SUMMARY. Since their introduction to the United States in the late 19th century, mute swans (Cygnus Olor) have become a nuisance species by causing damage to aquatic habitats, acting aggressively toward humans, competing with native waterfowl, and potentially transmitting or serving as a reservoir of infectious diseases to humans and poultry. In an effort to investigate their potential role as a disease reservoir and to establish avian health baselines for pathogens that threaten agricultural species or human health, we collected samples from 858 mute swans and tested them for avian paramyxovirus serotype 1 (APMV-1), avian influenza virus (AIV), and Salmonella spp. when possible. Our results indicate that exposure to APMV-1 and AIV is common (60%, n 5 771, and 45%, n 5 344, antibody prevalence, respectively) in mute swans, but detection of active viral shedding is less common (8.7%, n 5 414, and 0.8%, n 5 390, respectively). Salmonella was isolated from three mute swans (0.6%, n 5 459), and although the serovars identified have been implicated in previous human outbreaks, it does not appear that Salmonella is commonly carried by mute swans.
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Isolation and genetic characterization of Toxoplasma gondii from mute swan (Cygnus Olor) from the USA
Veterinary Parasitology, 2013Co-Authors: Jitender P. Dubey, S. Choudhary, Oliver C.h. Kwok, L.r. Ferreira, Solange Oliveira, Shiv K. Verma, David R. Marks, Kerri Pedersen, Randall M. Mickley, Adam R. RandallAbstract:a b s t r a c t Little is known of the genetic diversity of Toxoplasma gondii circulating in wildlife. In the present study, antibodies to T. gondii were determined in serum samples from 632 mute swans (Cygnus Olor) collected from different areas of the USA. Sera were tested by T. gondii modified agglutination test; 54 (8.5%) of 632 samples were seropositive with titers of 25 in 28 sera, 50 in 22 sera, 100 in three samples, and 200 or higher in one swan. Hearts from 14 seropositive swans were bioassayed in mice and viable T. gondii (designated TgSwanUs1-3) were isolated from the hearts of three. These three T. gondii isolates were further propa- gated in cell culture, and DNA isolated from culture-derived tachyzoites was characterized using 11 PCR-RFLP markers (SAG1, 5 � - and 3
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Isolation and genetic characterization of Toxoplasma gondii from mute swan (Cygnus Olor) from the USA.
Veterinary parasitology, 2013Co-Authors: Jitender P. Dubey, S. Choudhary, Oliver C.h. Kwok, L.r. Ferreira, Solange Oliveira, Shiv K. Verma, David R. Marks, Kerri Pedersen, Randall M. Mickley, Adam R. RandallAbstract:Little is known of the genetic diversity of Toxoplasma gondii circulating in wildlife. In the present study, antibodies to T. gondii were determined in serum samples from 632 mute swans (Cygnus Olor) collected from different areas of the USA. Sera were tested by T. gondii modified agglutination test; 54 (8.5%) of 632 samples were seropositive with titers of 25 in 28 sera, 50 in 22 sera, 100 in three samples, and 200 or higher in one swan. Hearts from 14 seropositive swans were bioassayed in mice and viable T. gondii (designated TgSwanUs1-3) were isolated from the hearts of three. These three T. gondii isolates were further propagated in cell culture, and DNA isolated from culture-derived tachyzoites was characterized using 11 PCR-RFLP markers (SAG1, 5'- and 3'-SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico). Results of genotyping revealed that two strains (TgSwanUs1, TgSwanUs2) were Type III (ToxoDB PCR-RFLP genotype #2), and TgSwanUs3 was a new genotype designated here as ToxoDB PCR-RFLP genotype #216. Pathogenicity of oocysts derived from these three strains was determined in Swiss Webster (SW) outbred mice. All mice infected with oocysts and tachyzoites of the atypical isolate (TgSwanUs3) died of acute toxoplasmosis, irrespective of the dose. Oocysts of the remaining two isolates were less pathogenic but differed from each other; 10 oocysts of the TgSwanUs1 killed all inoculated mice whereas 1 million oocysts of the TgSwanUs2 were needed to kill all infected SW mice. Isolation of T. gondii from mute swan indicates that the local waters were contaminated by T. gondii oocysts, and that mouse T. gondii virulent strains are circulating in wildlife. Mute swan is a new host record for T. gondii.
Randall M. Mickley - One of the best experts on this subject based on the ideXlab platform.
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Short report: Antibody prevalence of select arboviruses in mute swans (Cygnus Olor) in the Great Lakes region and Atlantic coast of the United States.
The American journal of tropical medicine and hygiene, 2014Co-Authors: Kerri Pedersen, David R. Marks, Randall M. Mickley, Dustin M. Arsnoe, Sarah N. Bevins, Eryu Wang, Scott C. Weaver, Thomas J. DelibertoAbstract:Mute swans (Cygnus Olor) are an invasive species in the United States. The dramatic increase in their populations in localized areas has led to various problems, among them competition with native species and attacks on humans by aggressive swans. However, very little is known about the ability of these swans to transmit pathogens to humans, domestic birds, or wildlife or participate in enzootic maintenance. To learn more about select pathogens that mute swans may harbor, a survey was conducted from April of 2011 to August of 2012 in the Great Lakes region and localized areas of the Atlantic coast, which revealed serologic evidence of arbovirus exposure in mute swans. Of 497 mute swans tested, antibodies were detected for eastern equine encephalitis (4.8%), St. Louis encephalitis (1.4%), West Nile (1.2%), and Turlock (0.6%) viruses. Samples were also tested for evidence of antibodies to La Crosse virus, but none were positive.
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Isolation and genetic characterization of Toxoplasma gondii from mute swan (Cygnus Olor) from the USA
Veterinary Parasitology, 2013Co-Authors: Jitender P. Dubey, S. Choudhary, Oliver C.h. Kwok, L.r. Ferreira, Solange Oliveira, Shiv K. Verma, David R. Marks, Kerri Pedersen, Randall M. Mickley, Adam R. RandallAbstract:a b s t r a c t Little is known of the genetic diversity of Toxoplasma gondii circulating in wildlife. In the present study, antibodies to T. gondii were determined in serum samples from 632 mute swans (Cygnus Olor) collected from different areas of the USA. Sera were tested by T. gondii modified agglutination test; 54 (8.5%) of 632 samples were seropositive with titers of 25 in 28 sera, 50 in 22 sera, 100 in three samples, and 200 or higher in one swan. Hearts from 14 seropositive swans were bioassayed in mice and viable T. gondii (designated TgSwanUs1-3) were isolated from the hearts of three. These three T. gondii isolates were further propa- gated in cell culture, and DNA isolated from culture-derived tachyzoites was characterized using 11 PCR-RFLP markers (SAG1, 5 � - and 3
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Isolation and genetic characterization of Toxoplasma gondii from mute swan (Cygnus Olor) from the USA.
Veterinary parasitology, 2013Co-Authors: Jitender P. Dubey, S. Choudhary, Oliver C.h. Kwok, L.r. Ferreira, Solange Oliveira, Shiv K. Verma, David R. Marks, Kerri Pedersen, Randall M. Mickley, Adam R. RandallAbstract:Little is known of the genetic diversity of Toxoplasma gondii circulating in wildlife. In the present study, antibodies to T. gondii were determined in serum samples from 632 mute swans (Cygnus Olor) collected from different areas of the USA. Sera were tested by T. gondii modified agglutination test; 54 (8.5%) of 632 samples were seropositive with titers of 25 in 28 sera, 50 in 22 sera, 100 in three samples, and 200 or higher in one swan. Hearts from 14 seropositive swans were bioassayed in mice and viable T. gondii (designated TgSwanUs1-3) were isolated from the hearts of three. These three T. gondii isolates were further propagated in cell culture, and DNA isolated from culture-derived tachyzoites was characterized using 11 PCR-RFLP markers (SAG1, 5'- and 3'-SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico). Results of genotyping revealed that two strains (TgSwanUs1, TgSwanUs2) were Type III (ToxoDB PCR-RFLP genotype #2), and TgSwanUs3 was a new genotype designated here as ToxoDB PCR-RFLP genotype #216. Pathogenicity of oocysts derived from these three strains was determined in Swiss Webster (SW) outbred mice. All mice infected with oocysts and tachyzoites of the atypical isolate (TgSwanUs3) died of acute toxoplasmosis, irrespective of the dose. Oocysts of the remaining two isolates were less pathogenic but differed from each other; 10 oocysts of the TgSwanUs1 killed all inoculated mice whereas 1 million oocysts of the TgSwanUs2 were needed to kill all infected SW mice. Isolation of T. gondii from mute swan indicates that the local waters were contaminated by T. gondii oocysts, and that mouse T. gondii virulent strains are circulating in wildlife. Mute swan is a new host record for T. gondii.
