The Experts below are selected from a list of 4701 Experts worldwide ranked by ideXlab platform
Makoto Kato - One of the best experts on this subject based on the ideXlab platform.
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odor guided bee pollinators of two endangered winter early spring blooming orchids Cymbidium kanran and Cymbidium goeringii in japan
Plant Species Biology, 2010Co-Authors: Kaoru Tsuji, Makoto KatoAbstract:We detected the pollinators of two endangered orchid species, Cymbidium kanran and Cymbidium goeringii, in their natural habitats. Cymbidium kanran blooms in early winter and the fragrant nectarless flowers were exclusively visited and pollinated by workers of the Japanese honeybee (Apis cerana japonica). The pollination system seems deceptive and contrasts with the honeybee drone pollination system observed in Cymbidium floribundum. Cymbidium goeringii blooms in early spring, and the faintly fragrant, nectarless flowers were visited and pollinated by males of Anthophora plumipes villosula, suggesting that the labellar papillae of the orchid flowers may be a reward for the bees.
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Odor‐guided bee pollinators of two endangered winter/early spring blooming orchids, Cymbidium kanran and Cymbidium goeringii, in Japan
Plant Species Biology, 2010Co-Authors: Kaoru Tsuji, Makoto KatoAbstract:We detected the pollinators of two endangered orchid species, Cymbidium kanran and Cymbidium goeringii, in their natural habitats. Cymbidium kanran blooms in early winter and the fragrant nectarless flowers were exclusively visited and pollinated by workers of the Japanese honeybee (Apis cerana japonica). The pollination system seems deceptive and contrasts with the honeybee drone pollination system observed in Cymbidium floribundum. Cymbidium goeringii blooms in early spring, and the faintly fragrant, nectarless flowers were visited and pollinated by males of Anthophora plumipes villosula, suggesting that the labellar papillae of the orchid flowers may be a reward for the bees.
Xiaoli Shu - One of the best experts on this subject based on the ideXlab platform.
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Development of Cymbidium ensifolium genic-SSR markers and their utility in genetic diversity and population structure analysis in Cymbidiums.
BMC genetics, 2014Co-Authors: Feng Jin, Liang Jin, Cheng Huang, Aaron Jackson, Xiaoli ShuAbstract:Background Cymbidium is a genus of 68 species in the orchid family, with extremely high ornamental value. Marker-assisted selection has proven to be an effective strategy in accelerating plant breeding for many plant species. Analysis of Cymbidiums genetic background by molecular markers can be of great value in assisting parental selection and breeding strategy design, however, in plants such as Cymbidiums limited genomic resources exist. In order to obtain efficient markers, we deep sequenced the C. ensifolium transcriptome to identify simple sequence repeats derived from gene regions (genic-SSR).
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development of Cymbidium ensifolium genic ssr markers and their utility in genetic diversity and population structure analysis in Cymbidiums
BMC Genetics, 2014Co-Authors: Feng Jin, Liang Jin, Cheng Huang, Aaron Jackson, Xiaoli ShuAbstract:Cymbidium is a genus of 68 species in the orchid family, with extremely high ornamental value. Marker-assisted selection has proven to be an effective strategy in accelerating plant breeding for many plant species. Analysis of Cymbidiums genetic background by molecular markers can be of great value in assisting parental selection and breeding strategy design, however, in plants such as Cymbidiums limited genomic resources exist. In order to obtain efficient markers, we deep sequenced the C. ensifolium transcriptome to identify simple sequence repeats derived from gene regions (genic-SSR). The 7,936 genic-SSR markers were identified. A total of 80 genic-SSRs were selected, and primers were designed according to their flanking sequences. Of the 80 genic-SSR primer sets, 62 were amplified in C. ensifolium successfully, and 55 showed polymorphism when cross-tested among 9 Cymbidium species comprising 59 accessions. Unigenes containing the 62 genic-SSRs were searched against Non-redundant (Nr), Gene Ontology database (GO), eukaryotic orthologous groups (KOGs) and Kyoto Encyclopedia of Genes and Genomes (KEGG) database. The search resulted in 53 matching Nr sequences, of which 39 had GO terms, 18 were assigned to KOGs, and 15 were annotated with KEGG. Genetic diversity and population structure were analyzed based on 55 polymorphic genic-SSR data among 59 accessions. The genetic distance averaged 0.3911, ranging from 0.016 to 0.618. The polymorphic index content (PIC) of 55 polymorphic markers averaged 0.407, ranging from 0.033 to 0.863. A model-based clustering analysis revealed that five genetic groups existed in the collection. Accessions from the same species were typically grouped together; however, C. goeringii accessions did not always form a separate cluster, suggesting that C. goeringii accessions were polyphyletic. The genic-SSR identified in this study constitute a set of markers that can be applied across multiple Cymbidium species and used for the evaluation of genetic relationships as well as qualitative and quantitative trait mapping studies. Genic-SSR’s coupled with the functional annotations provided by the unigenes will aid in mapping candidate genes of specific function.
Kaoru Tsuji - One of the best experts on this subject based on the ideXlab platform.
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odor guided bee pollinators of two endangered winter early spring blooming orchids Cymbidium kanran and Cymbidium goeringii in japan
Plant Species Biology, 2010Co-Authors: Kaoru Tsuji, Makoto KatoAbstract:We detected the pollinators of two endangered orchid species, Cymbidium kanran and Cymbidium goeringii, in their natural habitats. Cymbidium kanran blooms in early winter and the fragrant nectarless flowers were exclusively visited and pollinated by workers of the Japanese honeybee (Apis cerana japonica). The pollination system seems deceptive and contrasts with the honeybee drone pollination system observed in Cymbidium floribundum. Cymbidium goeringii blooms in early spring, and the faintly fragrant, nectarless flowers were visited and pollinated by males of Anthophora plumipes villosula, suggesting that the labellar papillae of the orchid flowers may be a reward for the bees.
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Odor‐guided bee pollinators of two endangered winter/early spring blooming orchids, Cymbidium kanran and Cymbidium goeringii, in Japan
Plant Species Biology, 2010Co-Authors: Kaoru Tsuji, Makoto KatoAbstract:We detected the pollinators of two endangered orchid species, Cymbidium kanran and Cymbidium goeringii, in their natural habitats. Cymbidium kanran blooms in early winter and the fragrant nectarless flowers were exclusively visited and pollinated by workers of the Japanese honeybee (Apis cerana japonica). The pollination system seems deceptive and contrasts with the honeybee drone pollination system observed in Cymbidium floribundum. Cymbidium goeringii blooms in early spring, and the faintly fragrant, nectarless flowers were visited and pollinated by males of Anthophora plumipes villosula, suggesting that the labellar papillae of the orchid flowers may be a reward for the bees.
Feng Jin - One of the best experts on this subject based on the ideXlab platform.
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Development of Cymbidium ensifolium genic-SSR markers and their utility in genetic diversity and population structure analysis in Cymbidiums.
BMC genetics, 2014Co-Authors: Feng Jin, Liang Jin, Cheng Huang, Aaron Jackson, Xiaoli ShuAbstract:Background Cymbidium is a genus of 68 species in the orchid family, with extremely high ornamental value. Marker-assisted selection has proven to be an effective strategy in accelerating plant breeding for many plant species. Analysis of Cymbidiums genetic background by molecular markers can be of great value in assisting parental selection and breeding strategy design, however, in plants such as Cymbidiums limited genomic resources exist. In order to obtain efficient markers, we deep sequenced the C. ensifolium transcriptome to identify simple sequence repeats derived from gene regions (genic-SSR).
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development of Cymbidium ensifolium genic ssr markers and their utility in genetic diversity and population structure analysis in Cymbidiums
BMC Genetics, 2014Co-Authors: Feng Jin, Liang Jin, Cheng Huang, Aaron Jackson, Xiaoli ShuAbstract:Cymbidium is a genus of 68 species in the orchid family, with extremely high ornamental value. Marker-assisted selection has proven to be an effective strategy in accelerating plant breeding for many plant species. Analysis of Cymbidiums genetic background by molecular markers can be of great value in assisting parental selection and breeding strategy design, however, in plants such as Cymbidiums limited genomic resources exist. In order to obtain efficient markers, we deep sequenced the C. ensifolium transcriptome to identify simple sequence repeats derived from gene regions (genic-SSR). The 7,936 genic-SSR markers were identified. A total of 80 genic-SSRs were selected, and primers were designed according to their flanking sequences. Of the 80 genic-SSR primer sets, 62 were amplified in C. ensifolium successfully, and 55 showed polymorphism when cross-tested among 9 Cymbidium species comprising 59 accessions. Unigenes containing the 62 genic-SSRs were searched against Non-redundant (Nr), Gene Ontology database (GO), eukaryotic orthologous groups (KOGs) and Kyoto Encyclopedia of Genes and Genomes (KEGG) database. The search resulted in 53 matching Nr sequences, of which 39 had GO terms, 18 were assigned to KOGs, and 15 were annotated with KEGG. Genetic diversity and population structure were analyzed based on 55 polymorphic genic-SSR data among 59 accessions. The genetic distance averaged 0.3911, ranging from 0.016 to 0.618. The polymorphic index content (PIC) of 55 polymorphic markers averaged 0.407, ranging from 0.033 to 0.863. A model-based clustering analysis revealed that five genetic groups existed in the collection. Accessions from the same species were typically grouped together; however, C. goeringii accessions did not always form a separate cluster, suggesting that C. goeringii accessions were polyphyletic. The genic-SSR identified in this study constitute a set of markers that can be applied across multiple Cymbidium species and used for the evaluation of genetic relationships as well as qualitative and quantitative trait mapping studies. Genic-SSR’s coupled with the functional annotations provided by the unigenes will aid in mapping candidate genes of specific function.
Bo Yang - One of the best experts on this subject based on the ideXlab platform.
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genetic diversity of wild Cymbidium goeringii orchidaceae populations from hubei based on inter simple sequence repeats analysis
Frontiers of Biology in China, 2007Co-Authors: Xiaohong Yao, Li Gao, Bo YangAbstract:Cymbidium goeringii is a diploid and nonrewarding, bumblebee-pollinated species, which is distributed in China, Japan and Korea Peninsula. This species is now highly endangered due to the mass collection and forest clearance in China. In the present study, we investigated the distribution of genetic variation within and between eleven populations of Cymbidium goeringii in central China by using Inter-simple sequence repeats (ISSR) markers. Eleven primers produced a total of 127 clear and reproducible bands of which 112 were polymorphic. High genetic diversity was detected in Cymbidium goeringii for both population level (P = 63.1%; He = 0.194 5) and species level (P = 88.2%; He = 0.262 8). A higher level of genetic differentiation was detected among populations (G ST = 0.244 0, F ST = 0.220 7) with Nei’s G ST analysis and analysis of molecular variance (AMOVA), and no correlation was found between geographical and genetic distance. Genetic drift rather than gene flow played an important role in forming the present population structure of Cymbidium goeringii. Limited gene flow among populations and gene drift increase the extinction risk of local populations. Some conservation concerns are therefore discussed together with possible strategies for implementing in situ and ex situ conservation.
