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Nallasivam Palanisamy - One of the best experts on this subject based on the ideXlab platform.
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sclerosing tfeb rearrangement renal cell carcinoma a recurring histologic pattern
Human Pathology, 2017Co-Authors: Sean R Williamson, John N Eble, Nallasivam PalanisamyAbstract:Renal cell carcinoma with TFEB rearrangement (t[6;11][p21;q13]) was initially recognized to be composed of dual populations of large cells with clear cytoplasm and small cells forming rosettes around hyaline material. With increasing awareness, however, the spectrum of described morphology has been found to be more heterogeneous. We report a 54-year-old woman who underwent partial nephrectomy for a 2.4-cm renal mass, composed of fibrosis, hyalinization, calcification, and ossification and a smaller component of epithelioid cells. Immunohistochemical staining revealed diffuse positivity for Cytokeratin AE1/AE3 and PAX8, patchy labeling for melan-A, human melanosome, and cathepsin K, and negative caldesmon, smooth muscle actin, TFE3 protein, carbonic anhydrase IX, CD10, Cytokeratin 7, epithelial membrane antigen, and inhibin. Fluorescence in situ hybridization confirmed rearrangement of TFEB and not TFE3. Together with one recent case in another report, our findings suggest that extensive sclerosis and ossification may be a less common recurring histology of TFEB-rearrangement renal cell carcinoma.
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Sclerosing TFEB-rearrangement renal cell carcinoma: a recurring histologic pattern ☆
Human Pathology, 2016Co-Authors: Sean R Williamson, John N Eble, Nallasivam PalanisamyAbstract:Renal cell carcinoma with TFEB rearrangement (t[6;11][p21;q13]) was initially recognized to be composed of dual populations of large cells with clear cytoplasm and small cells forming rosettes around hyaline material. With increasing awareness, however, the spectrum of described morphology has been found to be more heterogeneous. We report a 54-year-old woman who underwent partial nephrectomy for a 2.4-cm renal mass, composed of fibrosis, hyalinization, calcification, and ossification and a smaller component of epithelioid cells. Immunohistochemical staining revealed diffuse positivity for Cytokeratin AE1/AE3 and PAX8, patchy labeling for melan-A, human melanosome, and cathepsin K, and negative caldesmon, smooth muscle actin, TFE3 protein, carbonic anhydrase IX, CD10, Cytokeratin 7, epithelial membrane antigen, and inhibin. Fluorescence in situ hybridization confirmed rearrangement of TFEB and not TFE3. Together with one recent case in another report, our findings suggest that extensive sclerosis and ossification may be a less common recurring histology of TFEB-rearrangement renal cell carcinoma.
Sean R Williamson - One of the best experts on this subject based on the ideXlab platform.
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sclerosing tfeb rearrangement renal cell carcinoma a recurring histologic pattern
Human Pathology, 2017Co-Authors: Sean R Williamson, John N Eble, Nallasivam PalanisamyAbstract:Renal cell carcinoma with TFEB rearrangement (t[6;11][p21;q13]) was initially recognized to be composed of dual populations of large cells with clear cytoplasm and small cells forming rosettes around hyaline material. With increasing awareness, however, the spectrum of described morphology has been found to be more heterogeneous. We report a 54-year-old woman who underwent partial nephrectomy for a 2.4-cm renal mass, composed of fibrosis, hyalinization, calcification, and ossification and a smaller component of epithelioid cells. Immunohistochemical staining revealed diffuse positivity for Cytokeratin AE1/AE3 and PAX8, patchy labeling for melan-A, human melanosome, and cathepsin K, and negative caldesmon, smooth muscle actin, TFE3 protein, carbonic anhydrase IX, CD10, Cytokeratin 7, epithelial membrane antigen, and inhibin. Fluorescence in situ hybridization confirmed rearrangement of TFEB and not TFE3. Together with one recent case in another report, our findings suggest that extensive sclerosis and ossification may be a less common recurring histology of TFEB-rearrangement renal cell carcinoma.
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Sclerosing TFEB-rearrangement renal cell carcinoma: a recurring histologic pattern ☆
Human Pathology, 2016Co-Authors: Sean R Williamson, John N Eble, Nallasivam PalanisamyAbstract:Renal cell carcinoma with TFEB rearrangement (t[6;11][p21;q13]) was initially recognized to be composed of dual populations of large cells with clear cytoplasm and small cells forming rosettes around hyaline material. With increasing awareness, however, the spectrum of described morphology has been found to be more heterogeneous. We report a 54-year-old woman who underwent partial nephrectomy for a 2.4-cm renal mass, composed of fibrosis, hyalinization, calcification, and ossification and a smaller component of epithelioid cells. Immunohistochemical staining revealed diffuse positivity for Cytokeratin AE1/AE3 and PAX8, patchy labeling for melan-A, human melanosome, and cathepsin K, and negative caldesmon, smooth muscle actin, TFE3 protein, carbonic anhydrase IX, CD10, Cytokeratin 7, epithelial membrane antigen, and inhibin. Fluorescence in situ hybridization confirmed rearrangement of TFEB and not TFE3. Together with one recent case in another report, our findings suggest that extensive sclerosis and ossification may be a less common recurring histology of TFEB-rearrangement renal cell carcinoma.
John N Eble - One of the best experts on this subject based on the ideXlab platform.
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sclerosing tfeb rearrangement renal cell carcinoma a recurring histologic pattern
Human Pathology, 2017Co-Authors: Sean R Williamson, John N Eble, Nallasivam PalanisamyAbstract:Renal cell carcinoma with TFEB rearrangement (t[6;11][p21;q13]) was initially recognized to be composed of dual populations of large cells with clear cytoplasm and small cells forming rosettes around hyaline material. With increasing awareness, however, the spectrum of described morphology has been found to be more heterogeneous. We report a 54-year-old woman who underwent partial nephrectomy for a 2.4-cm renal mass, composed of fibrosis, hyalinization, calcification, and ossification and a smaller component of epithelioid cells. Immunohistochemical staining revealed diffuse positivity for Cytokeratin AE1/AE3 and PAX8, patchy labeling for melan-A, human melanosome, and cathepsin K, and negative caldesmon, smooth muscle actin, TFE3 protein, carbonic anhydrase IX, CD10, Cytokeratin 7, epithelial membrane antigen, and inhibin. Fluorescence in situ hybridization confirmed rearrangement of TFEB and not TFE3. Together with one recent case in another report, our findings suggest that extensive sclerosis and ossification may be a less common recurring histology of TFEB-rearrangement renal cell carcinoma.
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Sclerosing TFEB-rearrangement renal cell carcinoma: a recurring histologic pattern ☆
Human Pathology, 2016Co-Authors: Sean R Williamson, John N Eble, Nallasivam PalanisamyAbstract:Renal cell carcinoma with TFEB rearrangement (t[6;11][p21;q13]) was initially recognized to be composed of dual populations of large cells with clear cytoplasm and small cells forming rosettes around hyaline material. With increasing awareness, however, the spectrum of described morphology has been found to be more heterogeneous. We report a 54-year-old woman who underwent partial nephrectomy for a 2.4-cm renal mass, composed of fibrosis, hyalinization, calcification, and ossification and a smaller component of epithelioid cells. Immunohistochemical staining revealed diffuse positivity for Cytokeratin AE1/AE3 and PAX8, patchy labeling for melan-A, human melanosome, and cathepsin K, and negative caldesmon, smooth muscle actin, TFE3 protein, carbonic anhydrase IX, CD10, Cytokeratin 7, epithelial membrane antigen, and inhibin. Fluorescence in situ hybridization confirmed rearrangement of TFEB and not TFE3. Together with one recent case in another report, our findings suggest that extensive sclerosis and ossification may be a less common recurring histology of TFEB-rearrangement renal cell carcinoma.
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Immunohistochemical evidence for mesothelial origin of paratesticular adenomatoid tumour.
Histopathology, 2000Co-Authors: Brett Delahunt, John N Eble, John N. Nacey, A. ThorntonAbstract:Aims To investigate the histogenesis of paratesticular adenomatoid tumour by use of immunohistochemical markers for a variety of carcinomas and mesothelioma. Methods and results Immunohistochemical staining of sections from 12 cases of paratesticular adenomatoid tumour was undertaken using primary antibodies to antigens expressed by benign epithelial cells and carcinoma (Cytokeratin AE1/AE3, Cytokeratin 34sE12, epithelial membrane antigen, MOC-31, Ber-EP4, CEA, B72.3, LEA.135, Leu M1), stromal and vascular markers (vimentin, CD34, factor VIII), and mesothelioma-associated antigens (thrombomodulin, HBME-1, OC 125) and p53 protein. There was absence of immunohistochemical expression of epithelial/carcinoma markers MOC-31, Ber-EP4, CEA, B72.3, LEA.135, Leu M1 and to factor VIII and CD34. All tumours expressed Cytokeratin AE1/AE3, epithelial membrane antigen and vimentin, with weak expression of Cytokeratin 34sE12 in 25% of tumours. Each tumour showed expression of thrombomodulin, HBME-1 and OC 125 in a membranous distribution. p53 protein expression was not detected. Conclusions The immunohistochemical profile of paratesticular adenomatoid tumour is strongly supportive of a mesothelial cell origin.
Xianglei He - One of the best experts on this subject based on the ideXlab platform.
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a pelvic cellular solitary fibrous tumor with multifocal expression of Cytokeratin AE1 ae3
International Journal of Clinical and Experimental Pathology, 2015Co-Authors: Ming Zhao, Rong Huang, Xianglei HeAbstract:We read with great interest a recently published article by Lecoutere et al [1] entitled “multifocal Cytokeratin expression in pleural and abdominal malignant solitary fibrous tumor: an unusual diagnostic pitfall”. Authors showed three histologically and immunohistochemically well-documented cases of pleural and abdominal malignant solitary fibrous tumor (SFT) with unexpected multifocal expression of Cytokeratin AE1/AE3, which can cause significant diagnostic confusions for practical pathologists. We have recently encountered a case of cellular SFT of pelvic cavity which showed multifocal immunoreaction to AE1/AE3, an unexpected phenomenon for STF which could elicit a broad spectrum of differential diagnostic considerations in this clinical setting. The patient was a previously healthy 53-year-old woman, who was incidentally found to have a pelvic mass measuring 9-cm in greatest diameter by annual physical examination. Tumorectomy was proceeded. Histological examination showed a well-demarcated, encapsulated tumor with a tightly packed, patternless, occasionally hemangiopericytic arrangement of cytologically bland, fusiform to ovoid cells with indistinctive cytoplasmic borders, setting in a fibro-collagenous stroma (Figure 1A and and1B).1B). Mitotic activity was 4/50 high power filed, foci of necrosis were not noted. By immunohistochemistry, the tumor cells showed diffuse and strong positivity for Vimentin, CD34 (Figure 2), and bcl-2, as well as strong and patchy positivity for Cytokeratin AE1/AE3 (Figure 3A and and3B),3B), but no expression of smooth muscle actin, desmin, estrogen receptor, progesterone receptor, CD10, EMA, Cytokeratin 5/6, Cytokeratin 7, calretinin, D2-40, inhibin, or S100 protein. Ki67 stain decorated less than 5% tumor cells. Figure 1 Morphology appearance of a patternless, hemangiopericytic arrangement of tightly packed, cytologically bland tumor cells (A. HE B. H&E × 200). Figure 2 Diffuse strong expression for CD34 (CD34 × 200). Figure 3 Multifocal strong cytoplasmic immunoreactivity for Cytokeratin AE1/AE3 (A and B. Cytokeratin AE1/AE3 × 200). On the basis of the convincing morphologic appearances, together with the immunohistochemical profiles of CD34+, bcl-2+, and vimentin+, a diagnosis of cellular SFT of the pelvic cavity was rendered. With regard to the differential diagnoses of this tumor, the unforeseen strong positivity for Cytokeratin AE1/AE3, despite multifocal, together with the clinical setting of a pelvic tumor may point to sarcomatoid carcinoma, arising either from the urinary tracts or from the genital organs, sarcomatoid mesothelioma origining from the peritoneum, and synovial sarcoma as major differential diagnoses. However, absence of histologically malignant features and no expression of other epithelial markers (EMA, Cytokeratin 5/6, Cytokeratin 7) and mesothelial markers (D2-40, calretinin, Cytokeratin 5/6) in this tumor could readily excluded the possibility of a carcinoma or a mesothelioma. Synovial sarcoma often expressed vimentin and bcl-2, however, it usually lacks the expression of CD34 [2]. Aberrant Cytokeratin expression by fibroblastic/myofibroblastic soft tissue lesions is not uncommon, such as inflammatory myofibroblastic tumors [3], proliferative fasciitis [4], and so on. Anomalous Cytokeratin expression by SFT, usually limited and focal, has also been reported sporadically in the literature, most of which are documented in malignant cases [1,5,6]. Most recently, in a study by Barak et al [4] who investigated the immunoreactivity for calretinin and Cytokeratin in a large cohort of myofibroblastic tumors, 3 out of 27 (11%) SFTs showed focal positivity for Cytokeratin AE1/AE3 and Cytokeratin 18. For practical pathologists, increased awareness of such nonclassical immunophenotype in SFT, together with the application of ancillary studies to exclude other differential diagnoses is crucial in arriving at accurate diagnosis, particularly in needle biopsy specimens providing with tiny materials.
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A pelvic cellular solitary fibrous tumor with multifocal expression of Cytokeratin AE1/AE3
International Journal of Clinical and Experimental Pathology, 2015Co-Authors: Ming Zhao, Rong Huang, Xianglei HeAbstract:We read with great interest a recently published article by Lecoutere et al [1] entitled “multifocal Cytokeratin expression in pleural and abdominal malignant solitary fibrous tumor: an unusual diagnostic pitfall”. Authors showed three histologically and immunohistochemically well-documented cases of pleural and abdominal malignant solitary fibrous tumor (SFT) with unexpected multifocal expression of Cytokeratin AE1/AE3, which can cause significant diagnostic confusions for practical pathologists. We have recently encountered a case of cellular SFT of pelvic cavity which showed multifocal immunoreaction to AE1/AE3, an unexpected phenomenon for STF which could elicit a broad spectrum of differential diagnostic considerations in this clinical setting. The patient was a previously healthy 53-year-old woman, who was incidentally found to have a pelvic mass measuring 9-cm in greatest diameter by annual physical examination. Tumorectomy was proceeded. Histological examination showed a well-demarcated, encapsulated tumor with a tightly packed, patternless, occasionally hemangiopericytic arrangement of cytologically bland, fusiform to ovoid cells with indistinctive cytoplasmic borders, setting in a fibro-collagenous stroma (Figure 1A and and1B).1B). Mitotic activity was 4/50 high power filed, foci of necrosis were not noted. By immunohistochemistry, the tumor cells showed diffuse and strong positivity for Vimentin, CD34 (Figure 2), and bcl-2, as well as strong and patchy positivity for Cytokeratin AE1/AE3 (Figure 3A and and3B),3B), but no expression of smooth muscle actin, desmin, estrogen receptor, progesterone receptor, CD10, EMA, Cytokeratin 5/6, Cytokeratin 7, calretinin, D2-40, inhibin, or S100 protein. Ki67 stain decorated less than 5% tumor cells. Figure 1 Morphology appearance of a patternless, hemangiopericytic arrangement of tightly packed, cytologically bland tumor cells (A. HE B. H&E × 200). Figure 2 Diffuse strong expression for CD34 (CD34 × 200). Figure 3 Multifocal strong cytoplasmic immunoreactivity for Cytokeratin AE1/AE3 (A and B. Cytokeratin AE1/AE3 × 200). On the basis of the convincing morphologic appearances, together with the immunohistochemical profiles of CD34+, bcl-2+, and vimentin+, a diagnosis of cellular SFT of the pelvic cavity was rendered. With regard to the differential diagnoses of this tumor, the unforeseen strong positivity for Cytokeratin AE1/AE3, despite multifocal, together with the clinical setting of a pelvic tumor may point to sarcomatoid carcinoma, arising either from the urinary tracts or from the genital organs, sarcomatoid mesothelioma origining from the peritoneum, and synovial sarcoma as major differential diagnoses. However, absence of histologically malignant features and no expression of other epithelial markers (EMA, Cytokeratin 5/6, Cytokeratin 7) and mesothelial markers (D2-40, calretinin, Cytokeratin 5/6) in this tumor could readily excluded the possibility of a carcinoma or a mesothelioma. Synovial sarcoma often expressed vimentin and bcl-2, however, it usually lacks the expression of CD34 [2]. Aberrant Cytokeratin expression by fibroblastic/myofibroblastic soft tissue lesions is not uncommon, such as inflammatory myofibroblastic tumors [3], proliferative fasciitis [4], and so on. Anomalous Cytokeratin expression by SFT, usually limited and focal, has also been reported sporadically in the literature, most of which are documented in malignant cases [1,5,6]. Most recently, in a study by Barak et al [4] who investigated the immunoreactivity for calretinin and Cytokeratin in a large cohort of myofibroblastic tumors, 3 out of 27 (11%) SFTs showed focal positivity for Cytokeratin AE1/AE3 and Cytokeratin 18. For practical pathologists, increased awareness of such nonclassical immunophenotype in SFT, together with the application of ancillary studies to exclude other differential diagnoses is crucial in arriving at accurate diagnosis, particularly in needle biopsy specimens providing with tiny materials.
Alexander Quaas - One of the best experts on this subject based on the ideXlab platform.
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Tumor budding assessed according to the criteria of the International Tumor Budding Consensus Conference determines prognosis in resected esophageal adenocarcinoma
Virchows Archiv, 2020Co-Authors: Philipp Lohneis, Lena Hieggelke, Florian Gebauer, Markus Ball, Christiane Bruns, Reinhard Büttner, Heike Löser, Alexander QuaasAbstract:Only few studies examined the prognostic effect of tumor budding in esophageal adenocarcinomas so far. However, different quantification approaches were used, so results cannot be directly compared. Recently, the International Tumor Budding Consensus Conference (ITBCC) published consensus criteria for the evaluation of tumor budding in colorectal cancer, which we applied in our study. Hematoxylin and eosin (H&E) and Cytokeratin (AE1/AE3) stained whole tissue slides of 104 resected esophageal adenocarcinomas were evaluated. The mean count of tumor buds was analyzed in one high power field according to the ITBCC criteria and assigned to budding groups Bd1-3. Tumor budding was significantly associated with a worse overall survival. Regardless of the quantification approach, an increased number of tumor buds was significantly associated with reduced overall survival (OS) (H&E: HR = 1.05 (95% CI 1.029–1.073), p
