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Gang Peng - One of the best experts on this subject based on the ideXlab platform.
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a class ii restricted cd8γ13 t cell clone protects during chlamydia muridarum genital tract infection
The Journal of Infectious Diseases, 2020Co-Authors: Raymond M Johnson, Norma Olivaresstrank, Gang PengAbstract:: The T cell response to chlamydia genital tract infections in humans and mice is unusual because the majority of antigen-specific CD8 T cells are not class I-restricted, referred to as "unrestricted" or "atypical". We previously reported that a subset of unrestricted murine chlamydia-specific CD8 T cells had a Cytokine Polarization pattern that included IFN-ɣ and IL-13. Here we investigated the transcriptome of CD8ɣ13 T cells, comparing them to Tc1 clones using microarray analysis. That study revealed that CD8ɣ13 Polarization included IL-5 in addition to IFN-γ and IL-13. Adoptive transfer studies were performed with Tc1 clones and a CD8ɣ13 T cell clone to determine whether either influenced bacterial clearance or immunopathology during Chlamydia muridarum genital tract infections. To our surprise, an adoptively transferred CD8ɣ13 T cell clone was remarkably proficient at preventing chlamydia immunopathology while the multifunctional Tc1 clone did not enhance clearance or significantly alter immunopathology. Mapping studies with MHC class I- and class II-deficient splenocytes showed our previously published chlamydia-specific CD8 T cell clones are MHC class II-restricted. MHC class II-restricted CD8 T cells may play important roles in protection from intracellular pathogens that limit class I antigen presentation, or diminish CD4 T cell numbers or impair their function. METHODS: In this study, we investigated the transcriptome of CD8γ13 T cells, comparing them to Tc1 clones using microarray analysis. That study revealed that CD8γ13 Polarization included IL-5 in addition to IFN-γ and IL-13. Adoptive transfer studies were performed with Tc1 clones and a CD8γ13 T-cell clone to determine whether either influenced bacterial clearance or immunopathology during Chlamydia muridarum genital tract infections. RESULTS: To our surprise, an adoptively transferred CD8γ13 T-cell clone was remarkably proficient at preventing chlamydia immunopathology, whereas the multifunctional Tc1 clone did not enhance clearance or significantly alter immunopathology. Mapping studies with major histocompatibility complex (MHC) class I- and class II-deficient splenocytes showed our previously published chlamydia-specific CD8 T-cell clones are MHC class II restricted. CONCLUSIONS: The MHC class II-restricted CD8 T cells may play an important role in protection from intracellular pathogens that limit class I antigen presentation or diminish CD4 T-cell numbers or impair their function.
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a class ii restricted cd8γ13 t cell clone protects during chlamydia muridarum genital tract infection
The Journal of Infectious Diseases, 2020Co-Authors: Raymond M Johnson, Norma Olivaresstrank, Gang PengAbstract:BACKGROUND The T-cell response to chlamydia genital tract infections in humans and mice is unusual because the majority of antigen-specific CD8 T cells are not class I restricted (referred to here as "unrestricted" or "atypical"). We previously reported that a subset of unrestricted murine chlamydia-specific CD8 T cells had a Cytokine Polarization pattern that included interferon (IFN)-γ and interleukin (IL)-13. METHODS In this study, we investigated the transcriptome of CD8γ13 T cells, comparing them to Tc1 clones using microarray analysis. That study revealed that CD8γ13 Polarization included IL-5 in addition to IFN-γ and IL-13. Adoptive transfer studies were performed with Tc1 clones and a CD8γ13 T-cell clone to determine whether either influenced bacterial clearance or immunopathology during Chlamydia muridarum genital tract infections. RESULTS To our surprise, an adoptively transferred CD8γ13 T-cell clone was remarkably proficient at preventing chlamydia immunopathology, whereas the multifunctional Tc1 clone did not enhance clearance or significantly alter immunopathology. Mapping studies with major histocompatibility complex (MHC) class I- and class II-deficient splenocytes showed our previously published chlamydia-specific CD8 T-cell clones are MHC class II restricted. CONCLUSIONS The MHC class II-restricted CD8 T cells may play an important role in protection from intracellular pathogens that limit class I antigen presentation or diminish CD4 T-cell numbers or impair their function.
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a class ii restricted cd8ɣ13 t cell clone protects during chlamydia muridarum genital tract infection
The Journal of Infectious Diseases, 2020Co-Authors: Raymond M Johnson, Norma Olivaresstrank, Gang PengAbstract:The T cell response to chlamydia genital tract infections in humans and mice is unusual because the majority of antigen-specific CD8 T cells are not class I-restricted, referred to as "unrestricted" or "atypical". We previously reported that a subset of unrestricted murine chlamydia-specific CD8 T cells had a Cytokine Polarization pattern that included IFN- and IL-13. Here we investigated the transcriptome of CD813 T cells, comparing them to Tc1 clones using microarray analysis. That study revealed that CD813 Polarization included IL-5 in addition to IFN-gamma and IL-13. Adoptive transfer studies were performed with Tc1 clones and a CD813 T cell clone to determine whether either influenced bacterial clearance or immunopathology during Chlamydia muridarum genital tract infections. To our surprise, an adoptively transferred CD813 T cell clone was remarkably proficient at preventing chlamydia immunopathology while the multifunctional Tc1 clone did not enhance clearance or significantly alter immunopathology. Mapping studies with MHC class I- and class II-deficient splenocytes showed our previously published chlamydia-specific CD8 T cell clones are MHC class II-restricted. MHC class II-restricted CD8 T cells may play important roles in protection from intracellular pathogens that limit class I antigen presentation, or diminish CD4 T cell numbers or impair their function.
Raymond M Johnson - One of the best experts on this subject based on the ideXlab platform.
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a class ii restricted cd8γ13 t cell clone protects during chlamydia muridarum genital tract infection
The Journal of Infectious Diseases, 2020Co-Authors: Raymond M Johnson, Norma Olivaresstrank, Gang PengAbstract:: The T cell response to chlamydia genital tract infections in humans and mice is unusual because the majority of antigen-specific CD8 T cells are not class I-restricted, referred to as "unrestricted" or "atypical". We previously reported that a subset of unrestricted murine chlamydia-specific CD8 T cells had a Cytokine Polarization pattern that included IFN-ɣ and IL-13. Here we investigated the transcriptome of CD8ɣ13 T cells, comparing them to Tc1 clones using microarray analysis. That study revealed that CD8ɣ13 Polarization included IL-5 in addition to IFN-γ and IL-13. Adoptive transfer studies were performed with Tc1 clones and a CD8ɣ13 T cell clone to determine whether either influenced bacterial clearance or immunopathology during Chlamydia muridarum genital tract infections. To our surprise, an adoptively transferred CD8ɣ13 T cell clone was remarkably proficient at preventing chlamydia immunopathology while the multifunctional Tc1 clone did not enhance clearance or significantly alter immunopathology. Mapping studies with MHC class I- and class II-deficient splenocytes showed our previously published chlamydia-specific CD8 T cell clones are MHC class II-restricted. MHC class II-restricted CD8 T cells may play important roles in protection from intracellular pathogens that limit class I antigen presentation, or diminish CD4 T cell numbers or impair their function. METHODS: In this study, we investigated the transcriptome of CD8γ13 T cells, comparing them to Tc1 clones using microarray analysis. That study revealed that CD8γ13 Polarization included IL-5 in addition to IFN-γ and IL-13. Adoptive transfer studies were performed with Tc1 clones and a CD8γ13 T-cell clone to determine whether either influenced bacterial clearance or immunopathology during Chlamydia muridarum genital tract infections. RESULTS: To our surprise, an adoptively transferred CD8γ13 T-cell clone was remarkably proficient at preventing chlamydia immunopathology, whereas the multifunctional Tc1 clone did not enhance clearance or significantly alter immunopathology. Mapping studies with major histocompatibility complex (MHC) class I- and class II-deficient splenocytes showed our previously published chlamydia-specific CD8 T-cell clones are MHC class II restricted. CONCLUSIONS: The MHC class II-restricted CD8 T cells may play an important role in protection from intracellular pathogens that limit class I antigen presentation or diminish CD4 T-cell numbers or impair their function.
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a class ii restricted cd8γ13 t cell clone protects during chlamydia muridarum genital tract infection
The Journal of Infectious Diseases, 2020Co-Authors: Raymond M Johnson, Norma Olivaresstrank, Gang PengAbstract:BACKGROUND The T-cell response to chlamydia genital tract infections in humans and mice is unusual because the majority of antigen-specific CD8 T cells are not class I restricted (referred to here as "unrestricted" or "atypical"). We previously reported that a subset of unrestricted murine chlamydia-specific CD8 T cells had a Cytokine Polarization pattern that included interferon (IFN)-γ and interleukin (IL)-13. METHODS In this study, we investigated the transcriptome of CD8γ13 T cells, comparing them to Tc1 clones using microarray analysis. That study revealed that CD8γ13 Polarization included IL-5 in addition to IFN-γ and IL-13. Adoptive transfer studies were performed with Tc1 clones and a CD8γ13 T-cell clone to determine whether either influenced bacterial clearance or immunopathology during Chlamydia muridarum genital tract infections. RESULTS To our surprise, an adoptively transferred CD8γ13 T-cell clone was remarkably proficient at preventing chlamydia immunopathology, whereas the multifunctional Tc1 clone did not enhance clearance or significantly alter immunopathology. Mapping studies with major histocompatibility complex (MHC) class I- and class II-deficient splenocytes showed our previously published chlamydia-specific CD8 T-cell clones are MHC class II restricted. CONCLUSIONS The MHC class II-restricted CD8 T cells may play an important role in protection from intracellular pathogens that limit class I antigen presentation or diminish CD4 T-cell numbers or impair their function.
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a class ii restricted cd8ɣ13 t cell clone protects during chlamydia muridarum genital tract infection
The Journal of Infectious Diseases, 2020Co-Authors: Raymond M Johnson, Norma Olivaresstrank, Gang PengAbstract:The T cell response to chlamydia genital tract infections in humans and mice is unusual because the majority of antigen-specific CD8 T cells are not class I-restricted, referred to as "unrestricted" or "atypical". We previously reported that a subset of unrestricted murine chlamydia-specific CD8 T cells had a Cytokine Polarization pattern that included IFN- and IL-13. Here we investigated the transcriptome of CD813 T cells, comparing them to Tc1 clones using microarray analysis. That study revealed that CD813 Polarization included IL-5 in addition to IFN-gamma and IL-13. Adoptive transfer studies were performed with Tc1 clones and a CD813 T cell clone to determine whether either influenced bacterial clearance or immunopathology during Chlamydia muridarum genital tract infections. To our surprise, an adoptively transferred CD813 T cell clone was remarkably proficient at preventing chlamydia immunopathology while the multifunctional Tc1 clone did not enhance clearance or significantly alter immunopathology. Mapping studies with MHC class I- and class II-deficient splenocytes showed our previously published chlamydia-specific CD8 T cell clones are MHC class II-restricted. MHC class II-restricted CD8 T cells may play important roles in protection from intracellular pathogens that limit class I antigen presentation, or diminish CD4 T cell numbers or impair their function.
Norma Olivaresstrank - One of the best experts on this subject based on the ideXlab platform.
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a class ii restricted cd8γ13 t cell clone protects during chlamydia muridarum genital tract infection
The Journal of Infectious Diseases, 2020Co-Authors: Raymond M Johnson, Norma Olivaresstrank, Gang PengAbstract:: The T cell response to chlamydia genital tract infections in humans and mice is unusual because the majority of antigen-specific CD8 T cells are not class I-restricted, referred to as "unrestricted" or "atypical". We previously reported that a subset of unrestricted murine chlamydia-specific CD8 T cells had a Cytokine Polarization pattern that included IFN-ɣ and IL-13. Here we investigated the transcriptome of CD8ɣ13 T cells, comparing them to Tc1 clones using microarray analysis. That study revealed that CD8ɣ13 Polarization included IL-5 in addition to IFN-γ and IL-13. Adoptive transfer studies were performed with Tc1 clones and a CD8ɣ13 T cell clone to determine whether either influenced bacterial clearance or immunopathology during Chlamydia muridarum genital tract infections. To our surprise, an adoptively transferred CD8ɣ13 T cell clone was remarkably proficient at preventing chlamydia immunopathology while the multifunctional Tc1 clone did not enhance clearance or significantly alter immunopathology. Mapping studies with MHC class I- and class II-deficient splenocytes showed our previously published chlamydia-specific CD8 T cell clones are MHC class II-restricted. MHC class II-restricted CD8 T cells may play important roles in protection from intracellular pathogens that limit class I antigen presentation, or diminish CD4 T cell numbers or impair their function. METHODS: In this study, we investigated the transcriptome of CD8γ13 T cells, comparing them to Tc1 clones using microarray analysis. That study revealed that CD8γ13 Polarization included IL-5 in addition to IFN-γ and IL-13. Adoptive transfer studies were performed with Tc1 clones and a CD8γ13 T-cell clone to determine whether either influenced bacterial clearance or immunopathology during Chlamydia muridarum genital tract infections. RESULTS: To our surprise, an adoptively transferred CD8γ13 T-cell clone was remarkably proficient at preventing chlamydia immunopathology, whereas the multifunctional Tc1 clone did not enhance clearance or significantly alter immunopathology. Mapping studies with major histocompatibility complex (MHC) class I- and class II-deficient splenocytes showed our previously published chlamydia-specific CD8 T-cell clones are MHC class II restricted. CONCLUSIONS: The MHC class II-restricted CD8 T cells may play an important role in protection from intracellular pathogens that limit class I antigen presentation or diminish CD4 T-cell numbers or impair their function.
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a class ii restricted cd8γ13 t cell clone protects during chlamydia muridarum genital tract infection
The Journal of Infectious Diseases, 2020Co-Authors: Raymond M Johnson, Norma Olivaresstrank, Gang PengAbstract:BACKGROUND The T-cell response to chlamydia genital tract infections in humans and mice is unusual because the majority of antigen-specific CD8 T cells are not class I restricted (referred to here as "unrestricted" or "atypical"). We previously reported that a subset of unrestricted murine chlamydia-specific CD8 T cells had a Cytokine Polarization pattern that included interferon (IFN)-γ and interleukin (IL)-13. METHODS In this study, we investigated the transcriptome of CD8γ13 T cells, comparing them to Tc1 clones using microarray analysis. That study revealed that CD8γ13 Polarization included IL-5 in addition to IFN-γ and IL-13. Adoptive transfer studies were performed with Tc1 clones and a CD8γ13 T-cell clone to determine whether either influenced bacterial clearance or immunopathology during Chlamydia muridarum genital tract infections. RESULTS To our surprise, an adoptively transferred CD8γ13 T-cell clone was remarkably proficient at preventing chlamydia immunopathology, whereas the multifunctional Tc1 clone did not enhance clearance or significantly alter immunopathology. Mapping studies with major histocompatibility complex (MHC) class I- and class II-deficient splenocytes showed our previously published chlamydia-specific CD8 T-cell clones are MHC class II restricted. CONCLUSIONS The MHC class II-restricted CD8 T cells may play an important role in protection from intracellular pathogens that limit class I antigen presentation or diminish CD4 T-cell numbers or impair their function.
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a class ii restricted cd8ɣ13 t cell clone protects during chlamydia muridarum genital tract infection
The Journal of Infectious Diseases, 2020Co-Authors: Raymond M Johnson, Norma Olivaresstrank, Gang PengAbstract:The T cell response to chlamydia genital tract infections in humans and mice is unusual because the majority of antigen-specific CD8 T cells are not class I-restricted, referred to as "unrestricted" or "atypical". We previously reported that a subset of unrestricted murine chlamydia-specific CD8 T cells had a Cytokine Polarization pattern that included IFN- and IL-13. Here we investigated the transcriptome of CD813 T cells, comparing them to Tc1 clones using microarray analysis. That study revealed that CD813 Polarization included IL-5 in addition to IFN-gamma and IL-13. Adoptive transfer studies were performed with Tc1 clones and a CD813 T cell clone to determine whether either influenced bacterial clearance or immunopathology during Chlamydia muridarum genital tract infections. To our surprise, an adoptively transferred CD813 T cell clone was remarkably proficient at preventing chlamydia immunopathology while the multifunctional Tc1 clone did not enhance clearance or significantly alter immunopathology. Mapping studies with MHC class I- and class II-deficient splenocytes showed our previously published chlamydia-specific CD8 T cell clones are MHC class II-restricted. MHC class II-restricted CD8 T cells may play important roles in protection from intracellular pathogens that limit class I antigen presentation, or diminish CD4 T cell numbers or impair their function.
Athanasia Mouzaki - One of the best experts on this subject based on the ideXlab platform.
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citrullination of linear and cyclic altered peptide ligands from myelin basic protein mbp87 99 epitope elicits a th1 polarized response by t cells isolated from multiple sclerosis patients implications in triggering disease
Journal of Medicinal Chemistry, 2008Co-Authors: George Deraos, Kokona Chatzantoni, Minostimotheos Matsoukas, Theodore Tselios, Spyros Deraos, Maria Katsara, Panagiotis Papathanasopoulos, Demitrios Vynios, Vasso Apostolopoulos, Athanasia MouzakiAbstract:Derangement of cellular immunity is central in the pathophysiology of multiple sclerosis (MS) and is often manifested by abnormal Cytokine production. We investigated Cytokine secretion in peripheral blood mononuclear cells (PBMC) of 18 MS patients and 15 controls and correlated Cytokine Polarization with the nature of antigenic stimulus. We synthesized two novel citrullinated peptides, linear [Cit91, Ala96, Cit97]MBP87−99 and cyclo(87−99)[Cit91, Ala96, Cit97]MBP87−99 that resulted from citrullination of 91,97 Arg residues in antagonists, linear [Arg91, Ala96]MBP87−99 and cyclo(87−99)[Arg91, Ala96]MBP87−99 peptides. PBMC from MS patients and controls were cultured with citrullinated peptides, and both peptides caused a Th1 Polarization in all MS patients studied. In contrast, culture with noncitrullinated MBP peptides resulted in heterogeneous Cytokine secretion that differed between individual patients. Thus, citrullination of self-antigens may potentially trigger disease in susceptible individuals. This...
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adult chronic idiopathic thrombocytopenic purpura itp is the manifestation of a type 1 polarized immune response
Blood, 2004Co-Authors: Fotios Panitsas, Maria Theodoropoulou, Alexandra Kouraklis, Marina Karakantza, Georgios L Theodorou, N Zoumbos, Alice Maniatis, Athanasia MouzakiAbstract:Derangement of cellular immunity is central in the pathophysiology of adult autoimmune/idiopathic thrombocytopenic purpura (ITP). Herein we investigated Cytokine gene expression in peripheral blood mononuclear cells (PBMCs) of adult chronic ITP patients and attempted to correlate Cytokine Polarization with the degree of thrombocytopenia. We used semiquantitative reverse-transcriptase–polymerase chain reaction (RT-PCR) to measure the expression of type-1 (interleukin-2 [IL-2], interferon γ [IFN-γ]) and type-2 (IL-4, IL-5, IL-10, IL-3, IL-13) Cytokines by PBMCs from 21 patients and 11 controls. Plasma transforming growth factor β1 (TGF-β1) levels were measured by enzyme-linked immunoassay (ELISA). T helper 1 (Th1)/Th2 ([IL-2 + IFN-γ]/[IL-4 + IL-5]) Cytokine mRNA ratios, thought to reflect the Th deviation of the pathogenic disease-specific T cells, and type-1/type-2 mRNA ratios, thought to reflect the overall immune response Polarization, were significantly increased in ITP patients. The Th1/Th2 ratio was inversely correlated with platelet counts. TGF-β1 levels appeared suppressed in patients with active disease, though not significantly. Our findings show a clear type-1 Cytokine Polarization of the autoimmune response in adult ITP that persists irrespective of disease status.
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adult chronic idiopathic thrombocytopenic purpura itp is the manifestation of a type 1 polarized immune response
Blood, 2004Co-Authors: Fotios Panitsas, Maria Theodoropoulou, Alexandra Kouraklis, Marina Karakantza, Georgios L Theodorou, N Zoumbos, Alice Maniatis, Athanasia MouzakiAbstract:Derangement of cellular immunity is central in the pathophysiology of adult autoimmune/idiopathic thrombocytopenic purpura (ITP). Herein we investigated Cytokine gene expression in peripheral blood mononuclear cells (PBMCs) of adult chronic ITP patients and attempted to correlate Cytokine Polarization with the degree of thrombocytopenia. We used semiquantitative reverse-transcriptase-polymerase chain reaction (RT-PCR) to measure the expression of type-1 (interleukin-2 [IL-2], interferon gamma [IFN-gamma]) and type-2 (IL-4, IL-5, IL-10, IL-3, IL-13) Cytokines by PBMCs from 21 patients and 11 controls. Plasma transforming growth factor beta1 (TGF-beta1) levels were measured by enzyme-linked immunoassay (ELISA). T helper 1 (Th1)/Th2 ([IL-2 + IFN-gamma]/[IL-4 + IL-5]) Cytokine mRNA ratios, thought to reflect the Th deviation of the pathogenic disease-specific T cells, and type-1/type-2 mRNA ratios, thought to reflect the overall immune response Polarization, were significantly increased in ITP patients. The Th1/Th2 ratio was inversely correlated with platelet counts. TGF-beta1 levels appeared suppressed in patients with active disease, though not significantly. Our findings show a clear type-1 Cytokine Polarization of the autoimmune response in adult ITP that persists irrespective of disease status.
Helene Merleberal - One of the best experts on this subject based on the ideXlab platform.
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impaired th1 tc1 Cytokine production of tumor infiltrating lymphocytes in a model of primary intraocular b cell lymphoma
Investigative Ophthalmology & Visual Science, 2007Co-Authors: Valerie Touitou, Cecile Daussy, B Bodaghi, Serge Camelo, Yvonne De Kozak, P Lehoang, Mariechristine Naud, Audrey Varin, Brigitte Thillayegoldenberg, Helene MerleberalAbstract:PURPOSE. Primary intraocular lymphoma is a high-grade nonHodgkin lymphoma with a pathogenesis that is still unclear. Microenvironment is known to be crucial in controlling tumor growth and maintenance. To study the immune microenvironment in intraocular lymphomas and to characterize the Cytokine Polarization of infiltrating T-lymphocytes, a new murine model of intraocular B-cell lymphoma was developed. METHODS. Immunocompetent adult mice were injected intravitreally with a syngeneic lymphomatous B-cell line. Clinical, histologic, and flow cytometric analyses were performed to characterize the tumoral invasion and the immune infiltration. Cytokine production of ocular cells was investigated by RTPCR and fluorescent immunoassay, with or without stimulation by anti-CD3 anti-CD28 antibodies. RESULTS. Intraocular lymphoma developed in eyes injected by lymphomatous B-cells. At day 19, the retina and the vitreous cavity were infiltrated by tumor cells. Up to 15% of living cells were T-lymphocytes. Cytokine profile analysis of the supernatant of ocular cells cultured ex vivo demonstrated the presence of IL10, IL6, IFN, and TNF. Stimulation of ocular cells with anti-CD3 anti-CD28 antibodies increased the IFN level and led to the induction of IL2 production, completing the type 1 (Th1/Tc1-like) pattern of Cytokine expression observed. IL12p70 and IL4, potent Th1 or Th2 differentiating factors, were undetectable, even after stimulation. CONCLUSIONS. The results suggest that T-cells from intraocular B-lymphomas are characterized by a Th1/Tc1-like profile that could be partially inhibited in vivo. These data raise the possibility of a T-cell immunostimulation to reactivate the Th1/Tc1lymphocytes and improve intraocular antitumoral immunity. (Invest Ophthalmol Vis Sci. 2007;48:3223‐3229) DOI:
