The Experts below are selected from a list of 105 Experts worldwide ranked by ideXlab platform
Olivier Vandenberg - One of the best experts on this subject based on the ideXlab platform.
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Observation of a Cytopathogenic Effect on cell lines used for routine viral cultures led to the diagnosis of lymphogranuloma venereum.
Sexually transmitted infections, 2012Co-Authors: Laurent Busson, Tania Crucitti, Marc De Foor, Sigi Van Den Wijngaert, Olivier VandenbergAbstract:Objectives This article reports the fortuitous recovery of nine Chlamydia trachomatis serovar L strains in cell cultures (Vero and LLC-MK 2 cell line) designed for viral culture. Methods Nine ano-genital swabs were inoculated on confluent Vero, MRC5 and LLC-MK 2 cell cultures. They were collected from HIV-positive patients who were primarily men who have sex with men (MSM) presenting ulcerations that mimicked herpes simplex infections. Results A Cytopathogenic Effect was observed on Vero and LLC-MK 2 cells on day 14. The presence of C trachomatis serovar L in the cell lines was confirmed by Real Time–PCR. Conclusions C trachomatis serovar L can grow on Vero and LLC-MK 2 cell lines designed for viral cultures. Lymphogranuloma venereum must be considered as a differential diagnosis for herpes-like lesions, particularly in MSM with high-risk behaviours.
Laurent Busson - One of the best experts on this subject based on the ideXlab platform.
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Observation of a Cytopathogenic Effect on cell lines used for routine viral cultures led to the diagnosis of lymphogranuloma venereum.
Sexually transmitted infections, 2012Co-Authors: Laurent Busson, Tania Crucitti, Marc De Foor, Sigi Van Den Wijngaert, Olivier VandenbergAbstract:Objectives This article reports the fortuitous recovery of nine Chlamydia trachomatis serovar L strains in cell cultures (Vero and LLC-MK 2 cell line) designed for viral culture. Methods Nine ano-genital swabs were inoculated on confluent Vero, MRC5 and LLC-MK 2 cell cultures. They were collected from HIV-positive patients who were primarily men who have sex with men (MSM) presenting ulcerations that mimicked herpes simplex infections. Results A Cytopathogenic Effect was observed on Vero and LLC-MK 2 cells on day 14. The presence of C trachomatis serovar L in the cell lines was confirmed by Real Time–PCR. Conclusions C trachomatis serovar L can grow on Vero and LLC-MK 2 cell lines designed for viral cultures. Lymphogranuloma venereum must be considered as a differential diagnosis for herpes-like lesions, particularly in MSM with high-risk behaviours.
Monica Thelestam - One of the best experts on this subject based on the ideXlab platform.
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ADP-ribosylation in Clostridium difficile toxin-treated cells is not related to Cytopathogenicity of toxin B.
Biochimica et biophysica acta, 1991Co-Authors: Inger Florin, Monica ThelestamAbstract:ADP-ribosylation of a protein in human fibroblasts treated with partially purified Clostridium difficile toxin B was previously reported. Here we show that the same protein was ADP-ribosylated also in human fibroblasts exposed to supernatant from a C. difficile strain producing neither toxin A nor toxin B. Furthermore, in Chinese hamster ovary and in Vero cells, showing toxin B-induced Cytopathogenic Effect, the protein was not significantly ADP-ribosylated. The results indicate that the ADP-ribosylation is unrelated to the Cytopathogenic Effect of toxin B. It appears to be caused by another unidentified factor from C. difficile, and the substrate may correspond to a protein modified endogenously in cells exposed to stressful situations. Cellular actin was not ADP-ribosylated by toxin B.
Sigi Van Den Wijngaert - One of the best experts on this subject based on the ideXlab platform.
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Observation of a Cytopathogenic Effect on cell lines used for routine viral cultures led to the diagnosis of lymphogranuloma venereum.
Sexually transmitted infections, 2012Co-Authors: Laurent Busson, Tania Crucitti, Marc De Foor, Sigi Van Den Wijngaert, Olivier VandenbergAbstract:Objectives This article reports the fortuitous recovery of nine Chlamydia trachomatis serovar L strains in cell cultures (Vero and LLC-MK 2 cell line) designed for viral culture. Methods Nine ano-genital swabs were inoculated on confluent Vero, MRC5 and LLC-MK 2 cell cultures. They were collected from HIV-positive patients who were primarily men who have sex with men (MSM) presenting ulcerations that mimicked herpes simplex infections. Results A Cytopathogenic Effect was observed on Vero and LLC-MK 2 cells on day 14. The presence of C trachomatis serovar L in the cell lines was confirmed by Real Time–PCR. Conclusions C trachomatis serovar L can grow on Vero and LLC-MK 2 cell lines designed for viral cultures. Lymphogranuloma venereum must be considered as a differential diagnosis for herpes-like lesions, particularly in MSM with high-risk behaviours.
Marc De Foor - One of the best experts on this subject based on the ideXlab platform.
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Observation of a Cytopathogenic Effect on cell lines used for routine viral cultures led to the diagnosis of lymphogranuloma venereum.
Sexually transmitted infections, 2012Co-Authors: Laurent Busson, Tania Crucitti, Marc De Foor, Sigi Van Den Wijngaert, Olivier VandenbergAbstract:Objectives This article reports the fortuitous recovery of nine Chlamydia trachomatis serovar L strains in cell cultures (Vero and LLC-MK 2 cell line) designed for viral culture. Methods Nine ano-genital swabs were inoculated on confluent Vero, MRC5 and LLC-MK 2 cell cultures. They were collected from HIV-positive patients who were primarily men who have sex with men (MSM) presenting ulcerations that mimicked herpes simplex infections. Results A Cytopathogenic Effect was observed on Vero and LLC-MK 2 cells on day 14. The presence of C trachomatis serovar L in the cell lines was confirmed by Real Time–PCR. Conclusions C trachomatis serovar L can grow on Vero and LLC-MK 2 cell lines designed for viral cultures. Lymphogranuloma venereum must be considered as a differential diagnosis for herpes-like lesions, particularly in MSM with high-risk behaviours.
