The Experts below are selected from a list of 186 Experts worldwide ranked by ideXlab platform
Christopher J. Logan - One of the best experts on this subject based on the ideXlab platform.
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Structure–Activity Studies of Glucose Transfer: Determination of the Spontaneous Rates of Hydrolysis of Uridine 5′-Diphospho-α-d-glucose (UDPG) and Uridine 5′-diphospho-α-D-Glucuronic Acid (UDPGA)
Bioorganic & medicinal chemistry, 2003Co-Authors: Colin T. Bedford, Alan D Hickman, Christopher J. LoganAbstract:Abstract The pH-rate profiles for the hydrolysis of uridine 5′-diphospho-α- d -glucose (UDPG) and uridine 5′-diphospho-α- d -glucuronic Acid (UDPGA) in aqueous solution have been measured. The results obtained and a comparison with other data suggests that the mechanism of hydrolysis of each activated glycosyl-donor at pH 1–4 probably involves the slow ionisation, via an S N 1 process, of the neutral molecule to a glycosyl ion and UDP. From these data, the catalytic power ( k cat / k uncat ) of the glycosyltransferases has been estimated for the first time to be in the order of 10 11–13 .
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Mixed ion-pair high-performance liquid chromatography of uridine 5′-diphospho-α-D-Glucuronic Acid and its hydrolysis products
Journal of Chromatography A, 1995Co-Authors: Colin T. Bedford, Alan D Hickman, Christopher J. LoganAbstract:Abstract Although conventional ion-exchange HPLC and ion-pair reversed phase HPLC using tetrabutylammonium hydroxide allowed ready determination of uridine 5′-diphospho-α- d -glucose and its hydrolysis products, neither method was suitable for the determination of uridine 5′-diphospho-α- d -glucuronic Acid (α-UDPGA). However, mixed ion-pair reversed-phase HPLC, using a combination of tetrabutylammonium hydroxide and tetraethylammonium hydroxide (1:1), has been found to offer a convenient and effective means of simultaneously determining α-UDPGA and its hydrolysis products, UDP, UMP and cUMP.
Colin T. Bedford - One of the best experts on this subject based on the ideXlab platform.
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Structure–Activity Studies of Glucose Transfer: Determination of the Spontaneous Rates of Hydrolysis of Uridine 5′-Diphospho-α-d-glucose (UDPG) and Uridine 5′-diphospho-α-D-Glucuronic Acid (UDPGA)
Bioorganic & medicinal chemistry, 2003Co-Authors: Colin T. Bedford, Alan D Hickman, Christopher J. LoganAbstract:Abstract The pH-rate profiles for the hydrolysis of uridine 5′-diphospho-α- d -glucose (UDPG) and uridine 5′-diphospho-α- d -glucuronic Acid (UDPGA) in aqueous solution have been measured. The results obtained and a comparison with other data suggests that the mechanism of hydrolysis of each activated glycosyl-donor at pH 1–4 probably involves the slow ionisation, via an S N 1 process, of the neutral molecule to a glycosyl ion and UDP. From these data, the catalytic power ( k cat / k uncat ) of the glycosyltransferases has been estimated for the first time to be in the order of 10 11–13 .
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Mixed ion-pair high-performance liquid chromatography of uridine 5′-diphospho-α-D-Glucuronic Acid and its hydrolysis products
Journal of Chromatography A, 1995Co-Authors: Colin T. Bedford, Alan D Hickman, Christopher J. LoganAbstract:Abstract Although conventional ion-exchange HPLC and ion-pair reversed phase HPLC using tetrabutylammonium hydroxide allowed ready determination of uridine 5′-diphospho-α- d -glucose and its hydrolysis products, neither method was suitable for the determination of uridine 5′-diphospho-α- d -glucuronic Acid (α-UDPGA). However, mixed ion-pair reversed-phase HPLC, using a combination of tetrabutylammonium hydroxide and tetraethylammonium hydroxide (1:1), has been found to offer a convenient and effective means of simultaneously determining α-UDPGA and its hydrolysis products, UDP, UMP and cUMP.
Alan D Hickman - One of the best experts on this subject based on the ideXlab platform.
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Structure–Activity Studies of Glucose Transfer: Determination of the Spontaneous Rates of Hydrolysis of Uridine 5′-Diphospho-α-d-glucose (UDPG) and Uridine 5′-diphospho-α-D-Glucuronic Acid (UDPGA)
Bioorganic & medicinal chemistry, 2003Co-Authors: Colin T. Bedford, Alan D Hickman, Christopher J. LoganAbstract:Abstract The pH-rate profiles for the hydrolysis of uridine 5′-diphospho-α- d -glucose (UDPG) and uridine 5′-diphospho-α- d -glucuronic Acid (UDPGA) in aqueous solution have been measured. The results obtained and a comparison with other data suggests that the mechanism of hydrolysis of each activated glycosyl-donor at pH 1–4 probably involves the slow ionisation, via an S N 1 process, of the neutral molecule to a glycosyl ion and UDP. From these data, the catalytic power ( k cat / k uncat ) of the glycosyltransferases has been estimated for the first time to be in the order of 10 11–13 .
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Mixed ion-pair high-performance liquid chromatography of uridine 5′-diphospho-α-D-Glucuronic Acid and its hydrolysis products
Journal of Chromatography A, 1995Co-Authors: Colin T. Bedford, Alan D Hickman, Christopher J. LoganAbstract:Abstract Although conventional ion-exchange HPLC and ion-pair reversed phase HPLC using tetrabutylammonium hydroxide allowed ready determination of uridine 5′-diphospho-α- d -glucose and its hydrolysis products, neither method was suitable for the determination of uridine 5′-diphospho-α- d -glucuronic Acid (α-UDPGA). However, mixed ion-pair reversed-phase HPLC, using a combination of tetrabutylammonium hydroxide and tetraethylammonium hydroxide (1:1), has been found to offer a convenient and effective means of simultaneously determining α-UDPGA and its hydrolysis products, UDP, UMP and cUMP.
Lothar Elling - One of the best experts on this subject based on the ideXlab platform.
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Enzyme Module Systems for the Synthesis of Uridine 5′‐Diphospho‐α‐D‐glucuronic Acid and Non‐Sulfated Human Natural Killer Cell‐1 (HNK‐1) Epitope
Advanced Synthesis & Catalysis, 2015Co-Authors: Leonie Engels, Manja Henze, Werner Hummel, Lothar EllingAbstract:Tailor-made strategies for the stereo- and regioselective multi-step enzymatic synthesis of glycoconjugates require well characterized glycosyltransferases and carbohydrate modifying enzymes. We here report on a novel enzyme cascade for the synthesis of uridine 5'-diphospho-alpha-D-Glucuronic Acid (UDP-GlcA) and the non-sulfated human natural killer cell-1 (HNK-1) epitope including in situ regeneration of UDP-GlcA and the cofactor nicotinamide adenine dinucleotide NAD(+) by the combination of four enzymes in one-pot. In the first enzyme module sucrose synthase 1 (SuSy1) is used to produce uridine 5'-diphospho-alpha-D-glucose (UDP-Glc) from sucrose and uridine 5'-diphosphate (UDP). The combination with UDP-Glc dehydrogenase in the second enzyme module leads to the synthesis of UDP-GlcA with concomitant in situ regeneration of the cofactor NAD(+) by nicotinamide adenine dinucleotide hydride (NADH)-oxidase. In the third enzyme module the mammalian glucuronyltransferase GlcAT-P catalyzes the synthesis of the non-sulfated HNK-1 epitope by regioselective transfer of GlcA onto N-acetyllactosamine type 2 (LacNAc type 2). We present a comprehensive study on substrate kinetics, substrate specificities, variation and relation of enzyme activities as well as cross inhibition of intermediate products. With optimized reaction conditions we obtain superior product yields with streamlined synthesis costs for the expensive nucleotide sugar UDP-GlcA and cofactor NAD(+).
Tim Bowden - One of the best experts on this subject based on the ideXlab platform.
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selective michael type addition of a d glucuronic Acid derivative in the synthesis of model substances for uronic Acid containing polysaccharides
Express Polymer Letters, 2008Co-Authors: Kristoffer Bergman, Jons Hilborn, Tim BowdenAbstract:A flexible protocol for the preparation of model substances for uronic Acid containing polysaccharides is presented.We have synthesized a D-Glucuronic Acid derivative which is designed so that it e ...