Daidzein

14,000,000 Leading Edge Experts on the ideXlab platform

Scan Science and Technology

Contact Leading Edge Experts & Companies

Scan Science and Technology

Contact Leading Edge Experts & Companies

The Experts below are selected from a list of 7518 Experts worldwide ranked by ideXlab platform

Chengzhi Chai - One of the best experts on this subject based on the ideXlab platform.

  • simultaneous determination of puerarin daidzin Daidzein paeoniflorin albiflorin liquiritin and liquiritigenin in rat plasma and its application to a pharmacokinetic study of ge gen decoction by a liquid chromatography electrospray ionization tandem m
    Journal of Pharmaceutical and Biomedical Analysis, 2014
    Co-Authors: Chengzhi Chai, Dawei Wang, Jie Wu, Honghe Xiao, Boyang Yu
    Abstract:

    Abstract A liquid chromatography–electrospray ionization-tandem mass spectrometry (LC–ESI-MS/MS) method was developed and validated for simultaneous determination of seven constituents including puerarin, daidzin, Daidzein, paeoniflorin, albiflorin, liquiritin and liquiritigenin in rat plasma using schisandrin as the internal standard (IS). The plasma samples were pretreated by a one-step direct protein precipitation with acetonitrile. The chromatographic separation was carried out on a C18 column with a gradient mobile phase consisting of acetonitrile and water (containing 0.1% formic acid and 5 mM ammonium acetate). All analytes and IS were quantitated through electrospray ionization in positive ion multiple reaction monitoring (MRM) mode. The mass transitions were as follows: m/z 417.5 → 297.2 for puerarin, m/z 417.1 → 255.2 for daidzin, m/z 255.2 → 152.4 for Daidzein, m/z 498.1 → 179.3 for paeoniflorin, m/z 481.1 → 197.3 for albiflorin, m/z 436.2 → 257.3 for liquiritin, m/z 257.2 → 137.3 for liquiritigenin and m/z 415.0 → 384.2 for IS, respectively. All calibration curves exhibited good linearity (r > 0.9979) over a wide concentration range for all components. The intra-day and inter-day precisions (RSD) at three different levels were both less than 14.3% and the accuracies (RE) ranged from −13.2% to 14.8%. The extraction recoveries of the seven compounds ranged from 72.9% to 117.4%. The validated method was successfully applied to pharmacokinetic study of the seven components in female rat plasma after oral administration of Ge-Gen Decoction aqueous extract.

  • simultaneous determination of puerarin daidzin Daidzein paeoniflorin albiflorin liquiritin and liquiritigenin in rat plasma and its application to a pharmacokinetic study of ge gen decoction by a liquid chromatography electrospray ionization tandem m
    Journal of Pharmaceutical and Biomedical Analysis, 2014
    Co-Authors: Yan Yan, Chengzhi Chai, Dawei Wang, Honghe Xiao, Lixia Huo, Danni Zhu
    Abstract:

    Abstract A liquid chromatography–electrospray ionization-tandem mass spectrometry (LC–ESI-MS/MS) method was developed and validated for simultaneous determination of seven constituents including puerarin, daidzin, Daidzein, paeoniflorin, albiflorin, liquiritin and liquiritigenin in rat plasma using schisandrin as the internal standard (IS). The plasma samples were pretreated by a one-step direct protein precipitation with acetonitrile. The chromatographic separation was carried out on a C18 column with a gradient mobile phase consisting of acetonitrile and water (containing 0.1% formic acid and 5 mM ammonium acetate). All analytes and IS were quantitated through electrospray ionization in positive ion multiple reaction monitoring (MRM) mode. The mass transitions were as follows: m/z 417.5 → 297.2 for puerarin, m/z 417.1 → 255.2 for daidzin, m/z 255.2 → 152.4 for Daidzein, m/z 498.1 → 179.3 for paeoniflorin, m/z 481.1 → 197.3 for albiflorin, m/z 436.2 → 257.3 for liquiritin, m/z 257.2 → 137.3 for liquiritigenin and m/z 415.0 → 384.2 for IS, respectively. All calibration curves exhibited good linearity (r > 0.9979) over a wide concentration range for all components. The intra-day and inter-day precisions (RSD) at three different levels were both less than 14.3% and the accuracies (RE) ranged from −13.2% to 14.8%. The extraction recoveries of the seven compounds ranged from 72.9% to 117.4%. The validated method was successfully applied to pharmacokinetic study of the seven components in female rat plasma after oral administration of Ge-Gen Decoction aqueous extract.

Fuquan Yang - One of the best experts on this subject based on the ideXlab platform.

  • separation and purification of isoflavones from a crude soybean extract by high speed counter current chromatography
    Journal of Chromatography A, 2001
    Co-Authors: Fuquan Yang
    Abstract:

    A set of isoflavones with a broad range of polarity including daidzin, glycitin, genistin, acetyldaidzin, glycitein, acetylgenistin and Daidzein was separated from a crude soybean extract by high-speed counter-current chromatography using a two-step operation. Three solvent systems were used: chloroform–methanol–water (4:3:2, v/v); chloroform–methanol–n-butanol–water (4:3:0.5:2, v/v); and methyl tert.-butyl ether–tetrahydrofuran–0.5% aqueous trifluoroacetic acid (2:2:0.15:4, v/v). The first solvent system was used for separating less polar isoflavones and the second for more polar isoflavones by eluting the lower organic phase. Genistin and glycitin, which were only partially resolved in the chloroform system, were separated by the third solvent system. Each isolated component showed 98–99% purity as determined by high-performance liquid chromatography analysis. Their structures were identified by LC–MS.

  • separation and purification of isoflavones from a crude soybean extract by high speed counter current chromatography
    Journal of Chromatography A, 2001
    Co-Authors: Fuquan Yang
    Abstract:

    A set of isoflavones with a broad range of polarity including daidzin, glycitin, genistin, acetyldaidzin, glycitein, acetylgenistin and Daidzein was separated from a crude soybean extract by high-speed counter-current chromatography using a two-step operation. Three solvent systems were used: chloroform–methanol–water (4:3:2, v/v); chloroform–methanol–n-butanol–water (4:3:0.5:2, v/v); and methyl tert.-butyl ether–tetrahydrofuran–0.5% aqueous trifluoroacetic acid (2:2:0.15:4, v/v). The first solvent system was used for separating less polar isoflavones and the second for more polar isoflavones by eluting the lower organic phase. Genistin and glycitin, which were only partially resolved in the chloroform system, were separated by the third solvent system. Each isolated component showed 98–99% purity as determined by high-performance liquid chromatography analysis. Their structures were identified by LC–MS.

Danni Zhu - One of the best experts on this subject based on the ideXlab platform.

  • simultaneous determination of puerarin daidzin Daidzein paeoniflorin albiflorin liquiritin and liquiritigenin in rat plasma and its application to a pharmacokinetic study of ge gen decoction by a liquid chromatography electrospray ionization tandem m
    Journal of Pharmaceutical and Biomedical Analysis, 2014
    Co-Authors: Yan Yan, Chengzhi Chai, Dawei Wang, Honghe Xiao, Lixia Huo, Danni Zhu
    Abstract:

    Abstract A liquid chromatography–electrospray ionization-tandem mass spectrometry (LC–ESI-MS/MS) method was developed and validated for simultaneous determination of seven constituents including puerarin, daidzin, Daidzein, paeoniflorin, albiflorin, liquiritin and liquiritigenin in rat plasma using schisandrin as the internal standard (IS). The plasma samples were pretreated by a one-step direct protein precipitation with acetonitrile. The chromatographic separation was carried out on a C18 column with a gradient mobile phase consisting of acetonitrile and water (containing 0.1% formic acid and 5 mM ammonium acetate). All analytes and IS were quantitated through electrospray ionization in positive ion multiple reaction monitoring (MRM) mode. The mass transitions were as follows: m/z 417.5 → 297.2 for puerarin, m/z 417.1 → 255.2 for daidzin, m/z 255.2 → 152.4 for Daidzein, m/z 498.1 → 179.3 for paeoniflorin, m/z 481.1 → 197.3 for albiflorin, m/z 436.2 → 257.3 for liquiritin, m/z 257.2 → 137.3 for liquiritigenin and m/z 415.0 → 384.2 for IS, respectively. All calibration curves exhibited good linearity (r > 0.9979) over a wide concentration range for all components. The intra-day and inter-day precisions (RSD) at three different levels were both less than 14.3% and the accuracies (RE) ranged from −13.2% to 14.8%. The extraction recoveries of the seven compounds ranged from 72.9% to 117.4%. The validated method was successfully applied to pharmacokinetic study of the seven components in female rat plasma after oral administration of Ge-Gen Decoction aqueous extract.

Boyang Yu - One of the best experts on this subject based on the ideXlab platform.

  • simultaneous determination of puerarin daidzin Daidzein paeoniflorin albiflorin liquiritin and liquiritigenin in rat plasma and its application to a pharmacokinetic study of ge gen decoction by a liquid chromatography electrospray ionization tandem m
    Journal of Pharmaceutical and Biomedical Analysis, 2014
    Co-Authors: Chengzhi Chai, Dawei Wang, Jie Wu, Honghe Xiao, Boyang Yu
    Abstract:

    Abstract A liquid chromatography–electrospray ionization-tandem mass spectrometry (LC–ESI-MS/MS) method was developed and validated for simultaneous determination of seven constituents including puerarin, daidzin, Daidzein, paeoniflorin, albiflorin, liquiritin and liquiritigenin in rat plasma using schisandrin as the internal standard (IS). The plasma samples were pretreated by a one-step direct protein precipitation with acetonitrile. The chromatographic separation was carried out on a C18 column with a gradient mobile phase consisting of acetonitrile and water (containing 0.1% formic acid and 5 mM ammonium acetate). All analytes and IS were quantitated through electrospray ionization in positive ion multiple reaction monitoring (MRM) mode. The mass transitions were as follows: m/z 417.5 → 297.2 for puerarin, m/z 417.1 → 255.2 for daidzin, m/z 255.2 → 152.4 for Daidzein, m/z 498.1 → 179.3 for paeoniflorin, m/z 481.1 → 197.3 for albiflorin, m/z 436.2 → 257.3 for liquiritin, m/z 257.2 → 137.3 for liquiritigenin and m/z 415.0 → 384.2 for IS, respectively. All calibration curves exhibited good linearity (r > 0.9979) over a wide concentration range for all components. The intra-day and inter-day precisions (RSD) at three different levels were both less than 14.3% and the accuracies (RE) ranged from −13.2% to 14.8%. The extraction recoveries of the seven compounds ranged from 72.9% to 117.4%. The validated method was successfully applied to pharmacokinetic study of the seven components in female rat plasma after oral administration of Ge-Gen Decoction aqueous extract.

Honghe Xiao - One of the best experts on this subject based on the ideXlab platform.

  • simultaneous determination of puerarin daidzin Daidzein paeoniflorin albiflorin liquiritin and liquiritigenin in rat plasma and its application to a pharmacokinetic study of ge gen decoction by a liquid chromatography electrospray ionization tandem m
    Journal of Pharmaceutical and Biomedical Analysis, 2014
    Co-Authors: Chengzhi Chai, Dawei Wang, Jie Wu, Honghe Xiao, Boyang Yu
    Abstract:

    Abstract A liquid chromatography–electrospray ionization-tandem mass spectrometry (LC–ESI-MS/MS) method was developed and validated for simultaneous determination of seven constituents including puerarin, daidzin, Daidzein, paeoniflorin, albiflorin, liquiritin and liquiritigenin in rat plasma using schisandrin as the internal standard (IS). The plasma samples were pretreated by a one-step direct protein precipitation with acetonitrile. The chromatographic separation was carried out on a C18 column with a gradient mobile phase consisting of acetonitrile and water (containing 0.1% formic acid and 5 mM ammonium acetate). All analytes and IS were quantitated through electrospray ionization in positive ion multiple reaction monitoring (MRM) mode. The mass transitions were as follows: m/z 417.5 → 297.2 for puerarin, m/z 417.1 → 255.2 for daidzin, m/z 255.2 → 152.4 for Daidzein, m/z 498.1 → 179.3 for paeoniflorin, m/z 481.1 → 197.3 for albiflorin, m/z 436.2 → 257.3 for liquiritin, m/z 257.2 → 137.3 for liquiritigenin and m/z 415.0 → 384.2 for IS, respectively. All calibration curves exhibited good linearity (r > 0.9979) over a wide concentration range for all components. The intra-day and inter-day precisions (RSD) at three different levels were both less than 14.3% and the accuracies (RE) ranged from −13.2% to 14.8%. The extraction recoveries of the seven compounds ranged from 72.9% to 117.4%. The validated method was successfully applied to pharmacokinetic study of the seven components in female rat plasma after oral administration of Ge-Gen Decoction aqueous extract.

  • simultaneous determination of puerarin daidzin Daidzein paeoniflorin albiflorin liquiritin and liquiritigenin in rat plasma and its application to a pharmacokinetic study of ge gen decoction by a liquid chromatography electrospray ionization tandem m
    Journal of Pharmaceutical and Biomedical Analysis, 2014
    Co-Authors: Yan Yan, Chengzhi Chai, Dawei Wang, Honghe Xiao, Lixia Huo, Danni Zhu
    Abstract:

    Abstract A liquid chromatography–electrospray ionization-tandem mass spectrometry (LC–ESI-MS/MS) method was developed and validated for simultaneous determination of seven constituents including puerarin, daidzin, Daidzein, paeoniflorin, albiflorin, liquiritin and liquiritigenin in rat plasma using schisandrin as the internal standard (IS). The plasma samples were pretreated by a one-step direct protein precipitation with acetonitrile. The chromatographic separation was carried out on a C18 column with a gradient mobile phase consisting of acetonitrile and water (containing 0.1% formic acid and 5 mM ammonium acetate). All analytes and IS were quantitated through electrospray ionization in positive ion multiple reaction monitoring (MRM) mode. The mass transitions were as follows: m/z 417.5 → 297.2 for puerarin, m/z 417.1 → 255.2 for daidzin, m/z 255.2 → 152.4 for Daidzein, m/z 498.1 → 179.3 for paeoniflorin, m/z 481.1 → 197.3 for albiflorin, m/z 436.2 → 257.3 for liquiritin, m/z 257.2 → 137.3 for liquiritigenin and m/z 415.0 → 384.2 for IS, respectively. All calibration curves exhibited good linearity (r > 0.9979) over a wide concentration range for all components. The intra-day and inter-day precisions (RSD) at three different levels were both less than 14.3% and the accuracies (RE) ranged from −13.2% to 14.8%. The extraction recoveries of the seven compounds ranged from 72.9% to 117.4%. The validated method was successfully applied to pharmacokinetic study of the seven components in female rat plasma after oral administration of Ge-Gen Decoction aqueous extract.