The Experts below are selected from a list of 192 Experts worldwide ranked by ideXlab platform
Zhensheng Kang - One of the best experts on this subject based on the ideXlab platform.
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identification of expressed genes during compatible interaction between stripe rust puccinia striiformis and wheat using a cdna library
BMC Genomics, 2009Co-Authors: Xueling Huang, Xiaojie Wang, Xianming Chen, Zhipeng Qu, Lili Huang, Zhensheng KangAbstract:Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat worldwide. To establish compatibility with the host, Pst forms special infection structures to invade the plant with minimal damage to host cells. Although compatible interaction between wheat and Pst has been studied using various approaches, research on molecular mechanisms of the interaction is limited. The aim of this study was to develop an EST Database of wheat infected by Pst in order to determine transcription profiles of genes involved in compatible wheat-Pst interaction. Total RNA, extracted from susceptible infected wheat leaves harvested at 3, 5 and 8 days post inoculation (dpi), was used to create a cDNA library, from which 5,793 ESTs with high quality were obtained and clustered into 583 contigs and 2,160 singletons to give a set of 2,743 unisequences (GenBank accessions: GR302385 to GR305127). The BLASTx program was used to search for homologous genes of the unisequences in the GenBank non-redundant protein Database. Of the 2,743 unisequences, 52.8% (the largest category) were highly homologous to plant genes; 16.3% to fungal genes and 30% of no-hit. The functional classification of all ESTs was established based on the Database Entry giving the best E-value using the Bevan's classification categories. About 50% of the ESTs were significantly homologous to genes encoding proteins with known functions; 20% were similar to genes encoding proteins with unknown functions and 30% did not have significant homology to any sequence in the Database. The quantitative real-time PCR (qRT-PCR) analysis determined the transcription profiles and their involvement in the wheat-Pst interaction for seven of the gene. The cDNA library is useful for identifying the functional genes involved in the wheat-Pst compatible interaction, and established a new Database for studying Pst pathogenesis genes and wheat defense genes. The transcription patterns of seven genes were confirmed by the qRT-PCR assay to be differentially expressed in wheat-Pst compatible and incompatible interaction.
T.j. Jankun-kelly - One of the best experts on this subject based on the ideXlab platform.
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INFOVIS - Visualizing and Interacting with Multi-Tree Hierarchical Data
2004Co-Authors: M.j. Mohammadi-aragh, T.j. Jankun-kellyAbstract:This work focuses on visualizing highly cyclic hierarchical data. A user interface is discussed and its interaction is illustrated using a recipe Database example. This example showcases a Database with multiple categories for each recipe (Database Entry).
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Visualizing and Interacting with Multi-Tree Hierarchical Data
IEEE Symposium on Information Visualization, 2004Co-Authors: M.j. Mohammadi-aragh, T.j. Jankun-kellyAbstract:This work focuses on visualizing highly cyclic hierarchical data. A user interface is discussed and its interaction is illustrated using a recipe Database example. This example showcases a Database with multiple categories for each recipe (Database Entry).
M.j. Mohammadi-aragh - One of the best experts on this subject based on the ideXlab platform.
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INFOVIS - Visualizing and Interacting with Multi-Tree Hierarchical Data
2004Co-Authors: M.j. Mohammadi-aragh, T.j. Jankun-kellyAbstract:This work focuses on visualizing highly cyclic hierarchical data. A user interface is discussed and its interaction is illustrated using a recipe Database example. This example showcases a Database with multiple categories for each recipe (Database Entry).
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Visualizing and Interacting with Multi-Tree Hierarchical Data
IEEE Symposium on Information Visualization, 2004Co-Authors: M.j. Mohammadi-aragh, T.j. Jankun-kellyAbstract:This work focuses on visualizing highly cyclic hierarchical data. A user interface is discussed and its interaction is illustrated using a recipe Database example. This example showcases a Database with multiple categories for each recipe (Database Entry).
Michael D Ezekowitz - One of the best experts on this subject based on the ideXlab platform.
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a clinical computer Database Entry form for an intraoperative anesthesiology based transesophageal echocardiography monitoring service
Journal of Clinical Monitoring and Computing, 1993Co-Authors: Terence D Rafferty, E Davis, H Lippmann, Albert C Perrino, Stephen N Harris, Jane Carter, E K Prokop, Ira S Cohen, Michael D EzekowitzAbstract:Transesophageal echocardiography (TEE) has been increasingly applied to supplement and, in instances, to supplant conventional intraoperative cardiac monitoring. Our body of experience (>1600 intraoperative TEE procedures), combined with insights gleaned from an intramural quality assurance study, and clinical implications of certain recent advances in the field, led us to develop the following TEE computer Database Entry form. The form, completed intraoperatively, consists of a patient and surgical procedure demographics section, followed by fields based on the TEE examination. The scans encompass transverse plane basal short axis, long axis, and transgastric views of the heart and great vessels. The two-dimensional echocardiographic, saline-contrast, color flow and pulsed Doppler data represent both right and left ventricular performance, valvular function and specific lesions. This Database Entry form is intended to serve as a guide for performance of a nominally complete intraoperative study and facilitate maintenance of a TEE archive consistent with current advances.
Karl Reich - One of the best experts on this subject based on the ideXlab platform.
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automated forensic dna purification optimized for fta card punches and identifiler str based pcr analysis
Clinics in Laboratory Medicine, 2007Co-Authors: Lois C Tack, Michelle Thomas, Karl ReichAbstract:Forensic labs globally face the same problem—a growing need to process a greater number and wider variety of samples for DNA analysis. The same forensic lab can be tasked all at once with processing mixed casework samples from crime scenes, convicted offender samples for Database Entry, and tissue from tsunami victims for identification. Besides flexibility in the robotic system chosen for forensic automation, there is a need, for each sample type, to develop new methodology that is not only faster but also more reliable than past procedures. FTA is a chemical treatment of paper, unique to Whatman Bioscience, and is used for the stabilization and storage of biological samples. Here, the authors describe optimization of the Whatman FTA Purification Kit protocol for use with the AmpFlSTR Identifiler PCR Amplification Kit.
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automated forensic dna purification optimized for fta card punches and identifiler str based pcr analysis
Journal of Laboratory Automation, 2005Co-Authors: Lois C Tack, Michelle Thomas, Karl ReichAbstract:Forensic labs globally face the same problem—a growing need to process a greater number and wider variety of samples for DNA analysis. The same forensic lab can be tasked all at once with processing mixed casework samples from crime scenes, convicted offender samples for Database Entry, and tissue from tsunami victims for identification. Besides flexibility in the robotic system chosen for forensic automation, there is a need, for each sample type, to develop new methodology that is not only faster but also more reliable than past procedures. FTA is a chemical treatment of paper, unique to whatman Bioscience, and is used for the stabilization and storage of biological samples. Here, we describe optimization of the Whatman FTA Purification Kit protocol for use with the AmpFlSTR Identifiler PCR Amplification Kit. The conditions giving the best quality Identifiler results were used to automate a simple rapid method that processed forensic samples applied to FTA-treated paper punches in a 96-well plate in ∼30...
