The Experts below are selected from a list of 45 Experts worldwide ranked by ideXlab platform

Per Åman - One of the best experts on this subject based on the ideXlab platform.

  • Arabinoxylan fractionation on DEAE-Cellulose Chromatography influenced by protease pre-treatment
    Carbohydrate Polymers, 1999
    Co-Authors: Mathias Nilsson, Roger Andersson, Per Åman
    Abstract:

    An arabinoxylan mixture was extracted with saturated barium hydroxide from a water unextractable residue of rye bran. The mixture was fractionated on an anion exchange column which was eluted with water, 0.0025 M sodium borate, 0.025 M sodium borate and 0.4 M sodium hydroxide. It was possible to fractionate the arabinoxylan mixture into fractions with different structural features. The fractionation was repeated with prior protease treatment of the arabinoxylan mixture, but most of the arabinoxylan did not bind to the column by any mechanism that the protease treatment affected, As the largest fraction was still eluted with 0.4 M sodium hydroxide. Protease treatment affected the proportion of disubstituted xylose residues (dXyl) in the water, 0.0025 M sodium borate and 0.025 M sodium borate fractions, indicating that protein associated with arabinoxylans with a high dXyl content is more liable to the protease treatment or that protein is mainly associated with these structures. This study gives indications that protein is involved in the separation mechanism of rye arabinoxylan on a DEAE-Cellulose column.

Jean-louis Azanza - One of the best experts on this subject based on the ideXlab platform.

Luiz Claudio Miletti - One of the best experts on this subject based on the ideXlab platform.

  • Cryopreservation of Trypanosoma evansi after DEAE-Cellulose purification: Evaluation of infective parameters.
    Research in veterinary science, 2010
    Co-Authors: Kaio César Simano Tavares, Aleksandro S. Da Silva, Patrícia Wolkmer, Silvia Gonzalez Monteiro, Luiz Claudio Miletti
    Abstract:

    Cryopreservation is a method of keeping parasites alive in a laboratory. However, this technique may also damage the parasite. Alternatively, parasites may be maintained by in vitro culture. Unfortunately, for Trypanosoma evansi no effective medium that is able to maintain the parasite for more than 4 months has been described. In this study, we examined the effect of purifying trypomastigote through DEAE-Cellulose Chromatography before and after cryopreservation, by analyzing the pre-patent period, longevity, parasitemia, and count of viable parasites. Our results showed a three-times increase in the concentration of viable trypomastigote in DEAE-purified cryopreserved parasites as compared to non-DEAE-purified cryopreserved parasites. This indicates that DEAE-Cellulose Chromatography followed by cryopreservation is an effective method for the storage and preservation of T. evansi, with the advantage that the stocked parasites will be ready to use in molecular biology procedures.

Mathias Nilsson - One of the best experts on this subject based on the ideXlab platform.

  • Arabinoxylan fractionation on DEAE-Cellulose Chromatography influenced by protease pre-treatment
    Carbohydrate Polymers, 1999
    Co-Authors: Mathias Nilsson, Roger Andersson, Per Åman
    Abstract:

    An arabinoxylan mixture was extracted with saturated barium hydroxide from a water unextractable residue of rye bran. The mixture was fractionated on an anion exchange column which was eluted with water, 0.0025 M sodium borate, 0.025 M sodium borate and 0.4 M sodium hydroxide. It was possible to fractionate the arabinoxylan mixture into fractions with different structural features. The fractionation was repeated with prior protease treatment of the arabinoxylan mixture, but most of the arabinoxylan did not bind to the column by any mechanism that the protease treatment affected, As the largest fraction was still eluted with 0.4 M sodium hydroxide. Protease treatment affected the proportion of disubstituted xylose residues (dXyl) in the water, 0.0025 M sodium borate and 0.025 M sodium borate fractions, indicating that protein associated with arabinoxylans with a high dXyl content is more liable to the protease treatment or that protein is mainly associated with these structures. This study gives indications that protein is involved in the separation mechanism of rye arabinoxylan on a DEAE-Cellulose column.

Jacques Raymond - One of the best experts on this subject based on the ideXlab platform.