The Experts below are selected from a list of 5976 Experts worldwide ranked by ideXlab platform
Eileen Ingham - One of the best experts on this subject based on the ideXlab platform.
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development and characterization of an acellular porcine cartilage bone matrix for use in Tissue engineering
Journal of Biomedical Materials Research Part A, 2011Co-Authors: Ehab Kheir, Thomas Stapleton, J Fisher, David Shaw, Zhongmin Jin, Eileen InghamAbstract:The aim of this study was to develop a technique to decellularize a porcine cartilage bone construct with view to using this as a biological scaffold for cartilage substitution. The decellularization protocol applied freeze/thaw cycles; this was followed by cyclic incubation in hypotonic tris buffer and 0.1% (w/v) sodium dodecyl sulfate in hypotonic buffer plus protease inhibitors. Nucleases (RNase and DNase) were used to digest nucleic acids followed by disinfection using 0.1% (v/v) peracetic acid. Histological analysis confirmed the absence of visible cells within the Decellularized Tissue. DNA analysis revealed the near-complete removal of genomic DNA from the Decellularized Tissues. The decellularization process had minimal effect on the collagen content of the cartilage. However, there was a significant reduction in the glycosaminoglycan content in the Decellularized Tissues. There was no evidence of the expression of the major xenogeneic epitope, galactose-α-1,3-galactose. Biomechanical indentation testing of Decellularized Tissues showed a significant change in comparison to the fresh cartilage. This was presumed to be caused by the reduction in the glycosaminoglycan content. Biocompatibility of the acellular scaffold was determined using contact cytotoxicity assays and a galactosyltransferase knockout mouse model. Decellularized porcine cartilage Tissue was found to exhibit favorable compatibility in both in vitro and in vivo tests. In conclusion, this study has generated data on the production of an acellular cartilage bone matrix scaffold for use in osteochondral defect repair. To our knowledge, this is the first study that has successfully removed whole cells and α-gal from xenogeneic cartilage and bone Tissue.
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development and characterization of an acellular porcine cartilage bone matrix for use in Tissue engineering
Journal of Biomedical Materials Research Part A, 2011Co-Authors: Ehab Kheir, J Fisher, Thomas Stapleton, David Shaw, Eileen InghamAbstract:The aim of this study was to develop a technique to decellularize a porcine cartilage bone construct with view to using this as a biological scaffold for cartilage substitution. The decellularization protocol applied freeze/thaw cycles; this was followed by cyclic incubation in hypotonic tris buffer and 0.1% (w/v) sodium dodecyl sulfate in hypotonic buffer plus protease inhibitors. Nucleases (RNase and DNase) were used to digest nucleic acids followed by disinfection using 0.1% (v/v) peracetic acid. Histological analysis confirmed the absence of visible cells within the Decellularized Tissue. DNA analysis revealed the near-complete removal of genomic DNA from the Decellularized Tissues. The decellularization process had minimal effect on the collagen content of the cartilage. However, there was a significant reduction in the glycosaminoglycan content in the Decellularized Tissues. There was no evidence of the expression of the major xenogeneic epitope, galactose-α-1,3-galactose. Biomechanical indentation testing of Decellularized Tissues showed a significant change in comparison to the fresh cartilage. This was presumed to be caused by the reduction in the glycosaminoglycan content. Biocompatibility of the acellular scaffold was determined using contact cytotoxicity assays and a galactosyltransferase knockout mouse model. Decellularized porcine cartilage Tissue was found to exhibit favorable compatibility in both in vitro and in vivo tests. In conclusion, this study has generated data on the production of an acellular cartilage bone matrix scaffold for use in osteochondral defect repair. To our knowledge, this is the first study that has successfully removed whole cells and α-gal from xenogeneic cartilage and bone Tissue. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
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development and characterization of an acellular porcine medial meniscus for use in Tissue engineering
Tissue Engineering Part A, 2008Co-Authors: Thomas Stapleton, Joanne Ingram, Sotirios Korossis, Jaynath Katta, Richard L Knight, J Fisher, Eileen InghamAbstract:The objectives of this study were to characterize fresh porcine menisci and develop a decellularization protocol with a view to the generation of a biocompatible and biomechanically functional scaffold for use in Tissue engineering/regeneration of the meniscus. Menisci were Decellularized by exposing the Tissue to freeze-thaw cycles, incubation in hypotonic tris buffer, 0.1% (w/v) sodium dodecyl sulfate in hypotonic buffer plus protease inhibitors, nucleases, hypertonic buffer followed by disinfection using 0.1% (v/v) peracetic acid and final washing in phosphate-buffered saline. Histological, immunohistochemical, and biochemical analyses of the Decellularized Tissue confirmed the retention of the major structural proteins. There was, however, a 59.4% loss of glycosaminoglycans. The histoarchitecture was unchanged, and there was no evidence of the expression of the major xenogeneic epitope, galactose-α-1,3-galactose. Biocompatibility of the acellular scaffold was determined by using contact cytotoxicity a...
Thomas Stapleton - One of the best experts on this subject based on the ideXlab platform.
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development and characterization of an acellular porcine cartilage bone matrix for use in Tissue engineering
Journal of Biomedical Materials Research Part A, 2011Co-Authors: Ehab Kheir, Thomas Stapleton, J Fisher, David Shaw, Zhongmin Jin, Eileen InghamAbstract:The aim of this study was to develop a technique to decellularize a porcine cartilage bone construct with view to using this as a biological scaffold for cartilage substitution. The decellularization protocol applied freeze/thaw cycles; this was followed by cyclic incubation in hypotonic tris buffer and 0.1% (w/v) sodium dodecyl sulfate in hypotonic buffer plus protease inhibitors. Nucleases (RNase and DNase) were used to digest nucleic acids followed by disinfection using 0.1% (v/v) peracetic acid. Histological analysis confirmed the absence of visible cells within the Decellularized Tissue. DNA analysis revealed the near-complete removal of genomic DNA from the Decellularized Tissues. The decellularization process had minimal effect on the collagen content of the cartilage. However, there was a significant reduction in the glycosaminoglycan content in the Decellularized Tissues. There was no evidence of the expression of the major xenogeneic epitope, galactose-α-1,3-galactose. Biomechanical indentation testing of Decellularized Tissues showed a significant change in comparison to the fresh cartilage. This was presumed to be caused by the reduction in the glycosaminoglycan content. Biocompatibility of the acellular scaffold was determined using contact cytotoxicity assays and a galactosyltransferase knockout mouse model. Decellularized porcine cartilage Tissue was found to exhibit favorable compatibility in both in vitro and in vivo tests. In conclusion, this study has generated data on the production of an acellular cartilage bone matrix scaffold for use in osteochondral defect repair. To our knowledge, this is the first study that has successfully removed whole cells and α-gal from xenogeneic cartilage and bone Tissue.
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development and characterization of an acellular porcine cartilage bone matrix for use in Tissue engineering
Journal of Biomedical Materials Research Part A, 2011Co-Authors: Ehab Kheir, J Fisher, Thomas Stapleton, David Shaw, Eileen InghamAbstract:The aim of this study was to develop a technique to decellularize a porcine cartilage bone construct with view to using this as a biological scaffold for cartilage substitution. The decellularization protocol applied freeze/thaw cycles; this was followed by cyclic incubation in hypotonic tris buffer and 0.1% (w/v) sodium dodecyl sulfate in hypotonic buffer plus protease inhibitors. Nucleases (RNase and DNase) were used to digest nucleic acids followed by disinfection using 0.1% (v/v) peracetic acid. Histological analysis confirmed the absence of visible cells within the Decellularized Tissue. DNA analysis revealed the near-complete removal of genomic DNA from the Decellularized Tissues. The decellularization process had minimal effect on the collagen content of the cartilage. However, there was a significant reduction in the glycosaminoglycan content in the Decellularized Tissues. There was no evidence of the expression of the major xenogeneic epitope, galactose-α-1,3-galactose. Biomechanical indentation testing of Decellularized Tissues showed a significant change in comparison to the fresh cartilage. This was presumed to be caused by the reduction in the glycosaminoglycan content. Biocompatibility of the acellular scaffold was determined using contact cytotoxicity assays and a galactosyltransferase knockout mouse model. Decellularized porcine cartilage Tissue was found to exhibit favorable compatibility in both in vitro and in vivo tests. In conclusion, this study has generated data on the production of an acellular cartilage bone matrix scaffold for use in osteochondral defect repair. To our knowledge, this is the first study that has successfully removed whole cells and α-gal from xenogeneic cartilage and bone Tissue. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
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development and characterization of an acellular porcine medial meniscus for use in Tissue engineering
Tissue Engineering Part A, 2008Co-Authors: Thomas Stapleton, Joanne Ingram, Sotirios Korossis, Jaynath Katta, Richard L Knight, J Fisher, Eileen InghamAbstract:The objectives of this study were to characterize fresh porcine menisci and develop a decellularization protocol with a view to the generation of a biocompatible and biomechanically functional scaffold for use in Tissue engineering/regeneration of the meniscus. Menisci were Decellularized by exposing the Tissue to freeze-thaw cycles, incubation in hypotonic tris buffer, 0.1% (w/v) sodium dodecyl sulfate in hypotonic buffer plus protease inhibitors, nucleases, hypertonic buffer followed by disinfection using 0.1% (v/v) peracetic acid and final washing in phosphate-buffered saline. Histological, immunohistochemical, and biochemical analyses of the Decellularized Tissue confirmed the retention of the major structural proteins. There was, however, a 59.4% loss of glycosaminoglycans. The histoarchitecture was unchanged, and there was no evidence of the expression of the major xenogeneic epitope, galactose-α-1,3-galactose. Biocompatibility of the acellular scaffold was determined by using contact cytotoxicity a...
Ehab Kheir - One of the best experts on this subject based on the ideXlab platform.
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development and characterization of an acellular porcine cartilage bone matrix for use in Tissue engineering
Journal of Biomedical Materials Research Part A, 2011Co-Authors: Ehab Kheir, J Fisher, Thomas Stapleton, David Shaw, Eileen InghamAbstract:The aim of this study was to develop a technique to decellularize a porcine cartilage bone construct with view to using this as a biological scaffold for cartilage substitution. The decellularization protocol applied freeze/thaw cycles; this was followed by cyclic incubation in hypotonic tris buffer and 0.1% (w/v) sodium dodecyl sulfate in hypotonic buffer plus protease inhibitors. Nucleases (RNase and DNase) were used to digest nucleic acids followed by disinfection using 0.1% (v/v) peracetic acid. Histological analysis confirmed the absence of visible cells within the Decellularized Tissue. DNA analysis revealed the near-complete removal of genomic DNA from the Decellularized Tissues. The decellularization process had minimal effect on the collagen content of the cartilage. However, there was a significant reduction in the glycosaminoglycan content in the Decellularized Tissues. There was no evidence of the expression of the major xenogeneic epitope, galactose-α-1,3-galactose. Biomechanical indentation testing of Decellularized Tissues showed a significant change in comparison to the fresh cartilage. This was presumed to be caused by the reduction in the glycosaminoglycan content. Biocompatibility of the acellular scaffold was determined using contact cytotoxicity assays and a galactosyltransferase knockout mouse model. Decellularized porcine cartilage Tissue was found to exhibit favorable compatibility in both in vitro and in vivo tests. In conclusion, this study has generated data on the production of an acellular cartilage bone matrix scaffold for use in osteochondral defect repair. To our knowledge, this is the first study that has successfully removed whole cells and α-gal from xenogeneic cartilage and bone Tissue. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
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development and characterization of an acellular porcine cartilage bone matrix for use in Tissue engineering
Journal of Biomedical Materials Research Part A, 2011Co-Authors: Ehab Kheir, Thomas Stapleton, J Fisher, David Shaw, Zhongmin Jin, Eileen InghamAbstract:The aim of this study was to develop a technique to decellularize a porcine cartilage bone construct with view to using this as a biological scaffold for cartilage substitution. The decellularization protocol applied freeze/thaw cycles; this was followed by cyclic incubation in hypotonic tris buffer and 0.1% (w/v) sodium dodecyl sulfate in hypotonic buffer plus protease inhibitors. Nucleases (RNase and DNase) were used to digest nucleic acids followed by disinfection using 0.1% (v/v) peracetic acid. Histological analysis confirmed the absence of visible cells within the Decellularized Tissue. DNA analysis revealed the near-complete removal of genomic DNA from the Decellularized Tissues. The decellularization process had minimal effect on the collagen content of the cartilage. However, there was a significant reduction in the glycosaminoglycan content in the Decellularized Tissues. There was no evidence of the expression of the major xenogeneic epitope, galactose-α-1,3-galactose. Biomechanical indentation testing of Decellularized Tissues showed a significant change in comparison to the fresh cartilage. This was presumed to be caused by the reduction in the glycosaminoglycan content. Biocompatibility of the acellular scaffold was determined using contact cytotoxicity assays and a galactosyltransferase knockout mouse model. Decellularized porcine cartilage Tissue was found to exhibit favorable compatibility in both in vitro and in vivo tests. In conclusion, this study has generated data on the production of an acellular cartilage bone matrix scaffold for use in osteochondral defect repair. To our knowledge, this is the first study that has successfully removed whole cells and α-gal from xenogeneic cartilage and bone Tissue.
J Fisher - One of the best experts on this subject based on the ideXlab platform.
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development and characterization of an acellular porcine cartilage bone matrix for use in Tissue engineering
Journal of Biomedical Materials Research Part A, 2011Co-Authors: Ehab Kheir, Thomas Stapleton, J Fisher, David Shaw, Zhongmin Jin, Eileen InghamAbstract:The aim of this study was to develop a technique to decellularize a porcine cartilage bone construct with view to using this as a biological scaffold for cartilage substitution. The decellularization protocol applied freeze/thaw cycles; this was followed by cyclic incubation in hypotonic tris buffer and 0.1% (w/v) sodium dodecyl sulfate in hypotonic buffer plus protease inhibitors. Nucleases (RNase and DNase) were used to digest nucleic acids followed by disinfection using 0.1% (v/v) peracetic acid. Histological analysis confirmed the absence of visible cells within the Decellularized Tissue. DNA analysis revealed the near-complete removal of genomic DNA from the Decellularized Tissues. The decellularization process had minimal effect on the collagen content of the cartilage. However, there was a significant reduction in the glycosaminoglycan content in the Decellularized Tissues. There was no evidence of the expression of the major xenogeneic epitope, galactose-α-1,3-galactose. Biomechanical indentation testing of Decellularized Tissues showed a significant change in comparison to the fresh cartilage. This was presumed to be caused by the reduction in the glycosaminoglycan content. Biocompatibility of the acellular scaffold was determined using contact cytotoxicity assays and a galactosyltransferase knockout mouse model. Decellularized porcine cartilage Tissue was found to exhibit favorable compatibility in both in vitro and in vivo tests. In conclusion, this study has generated data on the production of an acellular cartilage bone matrix scaffold for use in osteochondral defect repair. To our knowledge, this is the first study that has successfully removed whole cells and α-gal from xenogeneic cartilage and bone Tissue.
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development and characterization of an acellular porcine cartilage bone matrix for use in Tissue engineering
Journal of Biomedical Materials Research Part A, 2011Co-Authors: Ehab Kheir, J Fisher, Thomas Stapleton, David Shaw, Eileen InghamAbstract:The aim of this study was to develop a technique to decellularize a porcine cartilage bone construct with view to using this as a biological scaffold for cartilage substitution. The decellularization protocol applied freeze/thaw cycles; this was followed by cyclic incubation in hypotonic tris buffer and 0.1% (w/v) sodium dodecyl sulfate in hypotonic buffer plus protease inhibitors. Nucleases (RNase and DNase) were used to digest nucleic acids followed by disinfection using 0.1% (v/v) peracetic acid. Histological analysis confirmed the absence of visible cells within the Decellularized Tissue. DNA analysis revealed the near-complete removal of genomic DNA from the Decellularized Tissues. The decellularization process had minimal effect on the collagen content of the cartilage. However, there was a significant reduction in the glycosaminoglycan content in the Decellularized Tissues. There was no evidence of the expression of the major xenogeneic epitope, galactose-α-1,3-galactose. Biomechanical indentation testing of Decellularized Tissues showed a significant change in comparison to the fresh cartilage. This was presumed to be caused by the reduction in the glycosaminoglycan content. Biocompatibility of the acellular scaffold was determined using contact cytotoxicity assays and a galactosyltransferase knockout mouse model. Decellularized porcine cartilage Tissue was found to exhibit favorable compatibility in both in vitro and in vivo tests. In conclusion, this study has generated data on the production of an acellular cartilage bone matrix scaffold for use in osteochondral defect repair. To our knowledge, this is the first study that has successfully removed whole cells and α-gal from xenogeneic cartilage and bone Tissue. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
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development and characterization of an acellular porcine medial meniscus for use in Tissue engineering
Tissue Engineering Part A, 2008Co-Authors: Thomas Stapleton, Joanne Ingram, Sotirios Korossis, Jaynath Katta, Richard L Knight, J Fisher, Eileen InghamAbstract:The objectives of this study were to characterize fresh porcine menisci and develop a decellularization protocol with a view to the generation of a biocompatible and biomechanically functional scaffold for use in Tissue engineering/regeneration of the meniscus. Menisci were Decellularized by exposing the Tissue to freeze-thaw cycles, incubation in hypotonic tris buffer, 0.1% (w/v) sodium dodecyl sulfate in hypotonic buffer plus protease inhibitors, nucleases, hypertonic buffer followed by disinfection using 0.1% (v/v) peracetic acid and final washing in phosphate-buffered saline. Histological, immunohistochemical, and biochemical analyses of the Decellularized Tissue confirmed the retention of the major structural proteins. There was, however, a 59.4% loss of glycosaminoglycans. The histoarchitecture was unchanged, and there was no evidence of the expression of the major xenogeneic epitope, galactose-α-1,3-galactose. Biocompatibility of the acellular scaffold was determined by using contact cytotoxicity a...
Boris Schmitt - One of the best experts on this subject based on the ideXlab platform.
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retraction bruder l et al transcatheter Decellularized Tissue engineered heart valve dtehv grown on polyglycolic acid pga scaffold coated with p4hb shows improved functionality over 52 weeks due to polyether ether ketone peek insert j funct biomater 2018 9 4 64
Journal of Functional Biomaterials, 2019Co-Authors: Leon Bruder, Kerstin Brakmann, Valentin Stegner, Matthias Sigler, Felix Berger, Boris SchmittAbstract:Due to human error, the authors included some of the experimental data in this article [...]
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transcatheter Decellularized Tissue engineered heart valve dtehv grown on polyglycolic acid pga scaffold coated with p4hb shows improved functionality over 52 weeks due to polyether ether ketone peek insert
Journal of Functional Biomaterials, 2018Co-Authors: Leon Bruder, Hendrik Spriestersbach, Kerstin Brakmann, Valentin Stegner, Matthias Sigler, Felix Berger, Boris SchmittAbstract:Many congenital heart defects and degenerative valve diseases require replacement of heart valves in children and young adults. Transcatheter xenografts degenerate over time. Tissue engineering might help to overcome this limitation by providing valves with ability for self-repair. A transcatheter Decellularized Tissue-engineered heart valve (dTEHV) was developed using a polyglycolic acid (PGA) scaffold. A first prototype showed progressive regurgitation after 6 months in-vivo due to a suboptimal design and misguided remodeling process. A new geometry was developed accordingly with computational fluid dynamics (CFD) simulations and implemented by adding a polyether-ether-ketone (PEEK) insert to the bioreactor during cultivation. This lead to more belly-shaped leaflets with higher coaptation areas for this second generation dTEHV. Valve functionality assessed via angiography, intracardiac echocardiography, and MRI proved to be much better when compared the first generation dTEHV, with preserved functionality up to 52 weeks after implantation. Macroscopic findings showed no thrombi or signs of acute inflammation. For the second generation dTEHV, belly-shaped leaflets with soft and agile Tissue-formation were seen after explantation. No excessive leaflet shortening occurred in the second generation dTEHV. Histological analysis showed complete engraftment of the dTEHV, with endothelialization of the leaflets and the graft wall. Leaflets consisted of collagenous Tissue and some elastic fibers. Adaptive leaflet remodeling was visible in all implanted second generation dTEHV, and most importantly no fusion between leaflet and wall was found. Very few remnants of the PGA scaffold were detected even 52 weeks after implantation, with no influence on functionality. By adding a polyether-ether-ketone (PEEK) insert to the bioreactor construct, a new geometry of PGA-scaffold based dTEHV could be implemented. This resulted in very good valve function of the implanted dTEHV over a period of 52 weeks.
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first percutaneous implantation of a completely Tissue engineered self expanding pulmonary heart valve prosthesis using a newly developed delivery system a feasibility study in sheep
Cardiovascular Intervention and Therapeutics, 2017Co-Authors: Hendrik Spriestersbach, Bart Sanders, Frank P T Baaijens, Felix Berger, Antonia Prudlo, Marco Bartosch, Torben Radtke, Simon P Hoerstrup, Boris SchmittAbstract:In a European consortium, a Decellularized Tissue-engineered heart valve (dTEHV) based on vessel-derived cells, a fast-degrading scaffold and a self-expanding stent has been developed. The aim of this study was to demonstrate that percutaneous delivery is feasible. To implant this valve prosthesis transcutaneously into pulmonary position, a catheter delivery system was designed and custom made. Three sheep underwent transjugular prototype implantation. Intracardiac echocardiography (ICE), angiography and computed tomography (CT) were applied to assess the position, morphology, function and dimensions of the stented dTEHV. One animal was killed 3 h after implantation and two animals were followed up for 12 weeks. Explanted valves were analyzed macroscopically and microscopically. In all animals, the percutaneous implantation of the stented dTEHV was successful. The prototype delivery system worked at first attempt in all animals. In the first implantation a 22 F system was used: the valve was slightly damaged during crimping. Loading was difficult due to valve-catheter mismatch in volume. In the second and third implantation a 26 F system was used: the valves fitted adequately and stayed intact. Following implantation, these two valves showed moderate regurgitation due to insufficient coaptation. During follow-up, regurgitation increased due to shortened leaflets. At explantation, macroscopic and microscopic analysis confirmed the second and third valve to be intact. Histology revealed autologous recellularization of the Decellularized valve after 12 weeks in vivo. It was demonstrated that completely in vitro Tissue-engineered heart valves are thin and stable enough to be crimped and implanted transvenously into pulmonary position.
